IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Neuron (0896-6273) 1998 Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. Drosophila head cDNA library used for screening. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. cell lysates used. cells cotransfected with both the constructs Drosophila head cDNA library used for screening. Neuron (0896-6273) 1998 Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Neuron (0896-6273) 1998 Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. Drosophila head cDNA library used for screening. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. Drosophila head cDNA library used for screening. Neuron (0896-6273) 1998 Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Neuron (0896-6273) 1998 Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. Drosophila head cDNA library used for screening. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. Slob-GFP fusion and anti dSlo antibodies were used for immunolocalisation. constructs were co transfected in cells. Drosophila head cDNA library used for screening. Neuron (0896-6273) 1998 Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Slob, a novel protein that interacts with the Slowpoke calcium-dependent potassium channel. Neuron (0896-6273) 1998 Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. Drosophila head cDNA library used for screening. drome-head Drosophila melanogaster head anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Schopperle WM., Holmqvist MH., Zhou Y., Wang J., Wang Z., Griffith LC., Keselman I., Kusinitz F., Dagan D., Levitan IB. Drosophila head lysate used for the experiment. Drosophila head cDNA library used for screening. Neuron (0896-6273) 1998 slob_drome Slowpoke-binding protein Slob CG43756 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFKFNKAAQQQRIDNRNSAVTGHDPFVRPPVPEKKVRNIMKKLHKANGLKRSNSAIEFDVSALTAANRRQIYSSNRSASSEQDNSDLSEHSEKSPLVSARLDNLARLFFSKSMPAETGSRDTIDSVLTTRPNIKYQYSALDSGNGIVERSPRERAQREKALNATQEWIQGANGRYEVIAHLDEIGSRHGKNWFLVTDASVRTDRLQTLLPLPPDCVAFEDLPPNECAREILMELLGSLHHPYIYPVLDLGFLRNSSYNYACLVTPFNSRGSLKDLIYKAQWNEPWARKYTRKPNGLPVSQVQRLGRQILEALLFLKERGFPLHGHLHSGNVILQNGAARLSGLENGLLGLSSRINAVMWSRSVTEIENVDIVCFGHLLYEMCTGQELTTPKPSMRVLEMELQHYPQIGQTRKQILEILGLIFEPPSGVCPSVEDLVLCDLFRSIDLRELRGPCFSTIKPSLSRSTLNLLQAVKKRQCASLGHSLSEANSPCTPPSTPHDRRTGVLPAPYEVFRMY 1B4D86959CCAFAC1 kcnae_drome Potassium voltage-gated channel protein eag eag Ether-a-go-go protein CG10952 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPGGRRGLVAPQNTFLENIIRRSNSQPDSSFLLANAQIVDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDITALKQPIDSEDTKGVLGLSKFAKLARSVTRSRQFSAHLPTLKDPTKQSNLAHMMSLSADIMPQYRQEAPKTPPHILLHYCAFKAIWDWVILCLTFYTAIMVPYNVAFKNKTSEDVSLLVVDSIVDVIFFIDIVLNFHTTFVGPGGEVVSDPKVIRMNYLKSWFIIDLLSCLPYDVFNAFDRDEDGIGSLFSALKVVRLLRLGRVVRKLDRYLEYGAAMLILLLCFYMLVAHWLACIWYSIGRSDADNGIQYSWLWKLANVTQSPYSYIWSNDTGPELVNGPSRKSMYVTALYFTMTCMTSVGFGNVAAETDNEKVFTICMMIIAALLYATIFGHVTTIIQQMTSATAKYHDMLNNVREFMKLHEVPKALSERVMDYVVSTWAMTKGLDTEKVLNYCPKDMKADICVHLNRKVFNEHPAFRLASDGCLRALAMHFMMSHSAPGDLLYHTGESIDSLCFIVTGSLEVIQDDEVVAILGKGDVFGDQFWKDSAVGQSAANVRALTYCDLHAIKRDKLLEVLDFYSAFANSFARNLVLTYNLRHRLIFRKVADVKREKELAERRKNEPQLPQNQDHLVRKIFSKFRRTPQVQAGSKELVGGSGQSDVEKGDGEVERTKVFPKAPKLQASQATLARQDTIDEGGEVDSSPPSRDSRVVIEGAAVSSATVGPSPPVATTSSAAAGAGVSGGPGSGGTVVAIVTKADRNLALERERQIEMASSRATTSDTYDTGLRETPPTLAQRDLIATVLDMKVDVRLELQRMQQRIGRIEDLLGELVKRLAPGAGSGGNAPDNSSGQTTPGDEICAGCGAGGGGTPTTQAPPTSAVTSPVDTVITISSPGASGSGSGTGAGAGSAVAGAGGAGLLNPGATVVSSAGGNGLGPLMLKKRRSKSRKAPAPPKQTLASTAGTATAAPAGVAGSGMTSSAPASADQQQQHQSTADQSPTTPGAELLHLRLLEEDFTAAQLPSTSSGGAGGGGGSGSGATPTTPPPTTAGGSGSGTPTSTTATTTPTGSGTATRGKLDFL B8F86ACBB5627FD3 slo_drome Calcium-activated potassium channel slowpoke Maxi K channel BK channel slo CG10693 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASGLIDTNFSSTLANGMSGCDQSTVESLADDPTDSPFDADDCLKVRKYWCFLLSSIFTFLAGLLVVLLWRAFAFVCCRKEPDLGPNDPKQKEQKASRNKQEFEGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFVDASSEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLDFNNAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPDMQSWTNDYLRGTGMEMYTETLSPTFIGIPFAQATELCFSKLKLLLLAIEIKGAEEGADSKISINPRGAKIQANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKNLTVQPRSKFDDLDEHHPAPTFTPPELPKRVHVRGSVSGDITRDREDTNLLNRNVRRPNGTGNGTGGMHHMNNTAAAAAAAAAAGKQVNKVKPTVNVSRQVEGQVISPSQYNRPTSRSSGTGTQNQNGGVSLPAGIADDQSKDFDFEKTEMKYDSTGMFHWSPAKSLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPTLADKEAILASLNIKAMTFDDTIGVLSQRGPEFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTVESLSNRDRCRVGQISLYDGPLAQFGECGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQFDPGLEYKPPAVRAPAGGRGTNTQGSGVGGGGSNKDDNS 306A12234C63AD0D q8iph9-2 Slowpoke-binding protein Slob CG43756 D protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFKFNKAAQQQRIDNRNSAVTGHDPFVRPPVPEKKVRNIMKKLHKANGLKRSNSAIEFDVSALTAANRRQIYSSNRSASSEQDNSDLSEHSEKSPLVSARLDNLARLFFSKSMPAETGSRDTIDSVLTTRPNIKYQYSALDSGNGIVERSPRERAQREKALNATQEWIQGANGRYEVIAHLDEIGSRHGKNWFLVTDASVRTDRLQTLLPLPPDCVAFEDLPPNECAREILMELLGSLHHPYIYPVLDLGFLRNSSYNYACLVTPFNSRGSLKDLIYKAQWNEPWARKYTRKPNGLPVSQVQRLGRQILEALLFLKERGFPLHGHLHSGNVILQNGAARLSGLENGLLGLSSRINAVMWSRSVTEIENVDIVCFGHLLYEMCTGQELTTPKPSMRVLEMELQHYPQIGQILEILGLIFEPPSGVCPSVEDLVLCDLFRSIDLRELRGPCFSTIKPSLSRSTLNLLQAVKKRQCASLGHSLSEANSPCTPPSTPHDRRTGVLPAPYEVFRMY FC9E6BE61A8B6A9B q8iph9-1 Slowpoke-binding protein Slob CG43756 A protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFKFNKAAQQQRIDNRNSAVTGHDPFVRPPVPEKKVRNIMKKLHKANGLKRSNSAIEFDVSALTAANRRQIYSSNRSASSEQDNSDLSEHSEKSPLVSARLDNLARLFFSKSMPAETGSRDTIDSVLTTRPNIKYQYSALDSGNGIVERSPRERAQREKALNATQEWIQGANGRYEVIAHLDEIGSRHGKNWFLVTDASVRTDRLQTLLPLPPDCVAFEDLPPNECAREILMELLGSLHHPYIYPVLDLGFLRNSSYNYACLVTPFNSRGSLKDLIYKAQWNEPWARKYTRKPNGLPVSQVQRLGRQILEALLFLKERGFPLHGHLHSGNVILQNGAARLSGLENGLLGLSSRINAVMWSRSVTEIENVDIVCFGHLLYEMCTGQELTTPKPSMRVLEMELQHYPQIGQTRKQILEILGLIFEPPSGVCPSVEDLVLCDLFRSIDLRELRGPCFSTIKPSLSRSTLNLLQAVKKRQCASLGHSLSEANSPCTPPSTPHDRRTGVLPAPYEVFRMY 1B4D86959CCAFAC1 slo-slobr 2 7 unspecified role unspecified role bait bait c terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey physical association physical association slo-slob-1 2 7 unspecified role unspecified role bait bait c terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association slo-slob-2 4 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association slo-slob-3 1 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association eag-slob 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association slo-slob-4 3 5 unspecified role unspecified role neutral component neutral component gfp tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component colocalization colocalization