DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies. The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies. Structure (0969-2126) 1996 imex curation Hasemann CA., Istvan ES., Uyeda K., Deisenhofer J. hasemann@howie.swmed.edu Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hasemann CA., Istvan ES., Uyeda K., Deisenhofer J. hasemann@howie.swmed.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Structure (0969-2126) 1996 IMEx f264_rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 Pfkfb4 6PF-2-K/Fru-2,6-P2ase testis-type isozyme protein protein rat Rattus norvegicus (Rat) Rat MASPRELTQNPLKKIWMPYSNGRPALHASQRGVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ E3D0AF34D3A09CA6 pfkfb4-1 1 2 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction working 18.3%, free 25.0% 2.00� Monomers associate in a head-to-head fashion with the 6-phosphofructo-2-kinase domain making contact. Sequence has all trytophans mutated to phenylalanine. Also His256Ala results in enzyme being catalytically inactive. Structure also shows position of ATPgammaS and inorganic phosphate in the molecule Revisit when Small Molecule Editor in place.