IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system. Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1980 Vora S., Seaman C., Durham S., Piomelli S. Complex identity made by antibody precipitation. Tetramers separated by affinity chromatography then subunit identity made by comparison to fractionation pattern of homotetramers. human-erythrocyte Homo sapiens erythrocyte Erythrocyte, Red blood cell; Corpuscle; RBC affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom polyclonal western polyclonal antibody western blot polyclonal western Vora S., Seaman C., Durham S., Piomelli S. Complex identity made by antibody precipitation. Tetramers separated by affinity chromatography then subunit identity made by comparison to fractionation pattern of homotetramers. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1980 Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system. Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1980 Vora S., Seaman C., Durham S., Piomelli S. Complex identity made by antibody precipitation. Tetramers separated by affinity chromatography then subunit identity made by comparison to fractionation pattern of homotetramers. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom polyclonal western polyclonal antibody western blot polyclonal western Vora S., Seaman C., Durham S., Piomelli S. Complex identity made by antibody precipitation. Tetramers separated by affinity chromatography then subunit identity made by comparison to fractionation pattern of homotetramers. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1980 pfkam_human ATP-dependent 6-phosphofructokinase, muscle type Phosphohexokinase Phosphofructo-1-kinase isozyme A PFKM 6-phosphofructokinase type A PFKX protein protein human Homo sapiens Human MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV 769A2C01F97D1122 pfkal_human ATP-dependent 6-phosphofructokinase, liver type PFKL Phosphohexokinase Phosphofructo-1-kinase isozyme B 6-phosphofructokinase type B protein protein human Homo sapiens Human MAAVDLEKLRASGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTIIGSARCKAFTTREGRRAAAYNLVQHGITNLCVIGGDGSLTGANIFRSEWGSLLEELVAEGKISETTARTYSHLNIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVTLSGNQSVRLPLMECVQMTKEVQKAMDDKRFDEATQLRGGSFENNWNIYKLLAHQKPPKEKSNFSLAILNVGAPAAGMNAAVRSAVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIHALLVVGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNEKCHDYYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAVAFSPVTELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF 0D686CE074E9626D pfkl-pfkl-3 1 4 unspecified role unspecified role neutral component neutral component physical association physical association pfkl-pfkm-1 1 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component physical association physical association pfkl-pfkm-2 1 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component physical association physical association pfkl-pfkm-3 1 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component physical association physical association pfkm-pfkm-3 1 3 unspecified role unspecified role neutral component neutral component physical association physical association pfkl-pfkl-2 2 4 unspecified role unspecified role neutral component neutral component human-liver Homo sapiens liver liver liver physical association physical association pfkl-pfkm-4 2 4 unspecified role unspecified role neutral component neutral component human-liver Homo sapiens liver liver liver 3 unspecified role unspecified role neutral component neutral component human-skeletal-muscle Homo sapiens skeletal muscle. skeletal_muscle Skeletal muscle physical association physical association pfkl-pfkm-5 2 3 unspecified role unspecified role neutral component neutral component human-skeletal-muscle Homo sapiens skeletal muscle. skeletal_muscle Skeletal muscle 4 unspecified role unspecified role neutral component neutral component human-liver Homo sapiens liver liver liver physical association physical association pfkm-pfkm-2 2 3 unspecified role unspecified role neutral component neutral component human-skeletal-muscle Homo sapiens skeletal muscle. skeletal_muscle Skeletal muscle physical association physical association