IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. Oncogene (0950-9232) 2006 imex curation Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Only protein-protein interactions human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. Oncogene (0950-9232) 2006 imex curation Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. Oncogene (0950-9232) 2006 imex curation Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Only protein-protein interactions human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. Oncogene (0950-9232) 2006 imex curation Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. Oncogene (0950-9232) 2006 imex curation Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. Oncogene (0950-9232) 2006 imex curation Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase Etk/BMX and protects human prostate cancer cells from apoptosis induced by chemotherapeutic drugs. Oncogene (0950-9232) 2006 imex curation Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Only protein-protein interactions human-22rv1 Homo sapiens 22rv1 Human prostate adenocarcinoma cell line Also known as CWR22Rv1 or Rv1. Cells dsplay a NE (neuroendocrine phenotype). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xie Y., Xu K., Dai B., Guo Z., Jiang T., Chen H., Qiu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cos-1 cells transfected and at 24 h post-transfection the cells were serum starved overnight. Participant detected by both anti-Flag and anti-phosphotyrosine blotting. 4a yqiu@som.umaryland.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Oncogene (0950-9232) 2006 Only protein-protein interactions imex curation bmx_human Cytoplasmic tyrosine-protein kinase BMX Bone marrow tyrosine kinase gene in chromosome X protein Epithelial and endothelial tyrosine kinase NTK38 BMX protein protein human Homo sapiens Human MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRSQWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVPDSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH CB22382A3BD02599 pim1_human Serine/threonine-protein kinase pim-1 PIM1 protein protein human Homo sapiens Human MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK 35BA76D3668E69A3 stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D DIP protein P40763 original sequence version: 157 DIP protein P40763-1 original sequence version: 147 DIP protein P40763-1 original sequence version: 135 DIP protein P40763-1 original sequence version: 157 DIP protein P40763 original sequence version: 165 DIP protein P40763 original sequence version: 182 DIP protein P40763 original sequence version: 135 6C00632211C8012D p11309-2 Serine/threonine-protein kinase pim-1 Pim-1L PIM1 protein protein human Homo sapiens Human MPHEPHEPLTPPFSALPDPAGAPSRRQSRQRPQLSSDSPSAFRASRSHSRNATRSHSHSHSPRHSLRHSPGSGSCGSSSGHRPCADILEVGMLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK Initiates from CTG codon. 18C421B7CFF87818 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 pim1-bmx-1 2 11 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4a, 4b BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). Author also performs experiments (Fig. 4B) to demonstrate binding of SH3 domain of BMX (ETK) to PIM1L. However, BMX does not contain an SH3 domain and sequence co-ordinates are not given, so this information has not been added. pim1-bmx-2 7 8 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4a BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). pim1-bmx-3 7 8 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, produced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). pim1-bmx-4 3 9 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association pim1-bmx-5 4 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association pim1-bmx-6 6 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey association association 4e Phosphorylation of BMX and Tyr-705 of STAT3 pim1-bmx-7 1 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain association association 4e Phosphorylation of BMX and Tyr-705 of STAT3 pim1-bmx-11 5 8 unspecified role unspecified role neutral component neutral component region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 3b BMX associates with PIM1L (UniProt identifier /FTId=PRO_0000043349) but not the shorter isoform PIM1S, prodiced by alternative promoter usage (UniProt identifier /FTId=PRO_0000043350). bmx-tp53-1 6 8 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association This interaction is disrupted by an assocation between PIM-1(L) and ETK