IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science (0036-8075) 2005 Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 2005 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science (0036-8075) 2005 Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 2005 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science (0036-8075) 2005 Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 2005 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science (0036-8075) 2005 Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 2005 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science (0036-8075) 2005 Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 2005 PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science (0036-8075) 2005 Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chipuk JE., Bouchier-Hayes L., Kuwana T., Newmeyer DD., Green DR. Apoptosis - Interactions involving proteins with a function related to apoptosis Prey immunoprecipitated using an agarose-conjugated antibody to Bcl-xL. Complex formed in response to DNA damage�induced apoptosis caused by 50 mJcmEXp-2 UV light 1A dgreen5240@aol.com Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 2005 bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG DIP protein Q07812 original sequence version: 73 DIP protein Q07812 original sequence version: 156 DIP protein Q07812 original sequence version: 92 DIP protein Q07812 original sequence version: 94 DIP protein Q07812-1 original sequence version: 93 DIP protein Q07812 original sequence version: 95 DIP protein Q07812 original sequence version: 120 DIP protein Q07812 original sequence version: 126 DIP protein Q07812 original sequence version: 143 DIP protein Q07812 original sequence version: 172 DIP protein Q07812 original sequence version: 175 6C0CDB0A7DEE4994 bbc3_human Bcl-2-binding component 3, isoforms 1/2 p53 up-regulated modulator of apoptosis JFY-1 BBC3 PUMA protein protein human Homo sapiens Human MARARQEGSSPEPVEGLARDGPRPFPLGRLVPSAVSCGLCEPGLAAAPAAPTLLPAAYLCAPTAPPAVTAALGGSRWPGGPRSRPRGPRPDGPQPSLSLAEQHLESPVPSAPGALAGGPTQAAPGVRGEEEQWAREIGAQLRRMADDLNAQYERRRQEEQQRHRPSPWRVLYNLIMGLLPLPRGHRAPEMEPN DIP protein Q9BXH1 original sequence version: 32 3585527F1858A4FA p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 bbc3_mouse Bcl-2-binding component 3 Bbc3 Puma p53 up-regulated modulator of apoptosis protein protein mouse Mus musculus Mouse MARARQEGSSPEPVEGLARDSPRPFPLGRLMPSAVSCSLCEPGLPAAPAAPALLPAAYLCAPTAPPAVTAALGGPRWPGGHRSRPRGPRPDGPQPSLSPAQQHLESPVPSAPEALAGGPTQAAPGVRVEEEEWAREIGAQLRRMADDLNAQYERRRQEEQHRHRPSPWRVMYNLFMGLLPLPRDPGAPEMEPN DIP protein Q99ML1 original sequence version: 37 39A98487FA86D226 q07817-1 Bcl-2-like protein 1 BCLX Apoptosis regulator Bcl-X BCL2L1 BCL2L Bcl-xL protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK DIP protein Q07817 original sequence version: 82 DIP protein Q07817 original sequence version: 86 DIP protein Q07817-1 original sequence version: 100 DIP protein Q07817-1 original sequence version: 108 DIP protein Q07817 original sequence version: 131 DIP protein Q07817-1 original sequence version: 110 DIP protein Q07817-1 original sequence version: 135 DIP protein Q07817-1 original sequence version: 131 DIP protein Q07817-1 original sequence version: 172 DIP protein Q07817-1 original sequence version: 174 DIP protein Q07817 original sequence version: 174 DIP protein Q07817-1 original sequence version: 189 DIP protein Q07817-1 original sequence version: 192 DIP protein Q07817 original sequence version: 190 E09D3CDD851AE9BE q64373-1 Bcl-2-like protein 1 Bcl2l1 Bcl2l Bclx Apoptosis regulator Bcl-X Bcl-xL protein protein mouse Mus musculus Mouse MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK 24D2AC79887E072E p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 tp53-bbc3-1 4 12 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association tp53-bbc3-2 5 12 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association tp53-bbc3-3 6 11 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey physical association physical association BBC3+/+ cells tp53-bclxl-1 6 11 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey physical association physical association BBC3 (PUMA)-/- cells tp53-bclxl-2 3 11 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association BBC3(PUMA) BH3 peptide bbc3-bclxl-4 2 11 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey direct interaction direct interaction tp53-bclxl-3 2 11 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey direct interaction direct interaction bbc3-bclxl-5 1 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A Wed May 02 14:59:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly138ala' Tue Jun 30 15:35:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly138Ala' direct interaction direct interaction bax-bclxl-1 1 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A Wed May 02 19:58:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly138ala' Tue Jun 30 16:52:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly138Ala' direct interaction direct interaction tp53-bclxl-4 3 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A Wed May 02 18:20:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly138ala' Tue Jun 30 15:35:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly138Ala' physical association physical association tp53-bbc3-4 5 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association tp53-bclxl-5 5 11 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association BBC3 -/- cells used as host organism tp53-bbc3-5 5 11 unspecified role unspecified role bait bait Q07817-1:p.Gly138Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G A Wed May 02 14:58:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly138ala' Tue Jun 30 20:11:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly138Ala' 10 unspecified role unspecified role prey prey physical association physical association