IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ LdpA: a component of the circadian clock senses redox state of the cell. LdpA: a component of the circadian clock senses redox state of the cell. EMBO J. (0261-4189) 2005 Ivleva NB., Bramlett MR., Lindahl PA., Golden SS. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species strain AMC1239 overexpressing His-tagged LdpA were used fig 3, fig S1, fig S2 sgolden@tamu.edu syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 pull down pull down western blot western blot Immuno blot Immuno blot Ivleva NB., Bramlett MR., Lindahl PA., Golden SS. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species strain AMC1239 overexpressing His-tagged LdpA were used fig 3, fig S1, fig S2 sgolden@tamu.edu EMBO J. (0261-4189) 2005 sasa_syne7 Adaptive-response sensory-kinase SasA Synechococcus adaptive sensor protein A sasA sarS Synpcc7942_2114 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MGESLSPQALAQPLLLQLFVDTRPLSQHIVQRVKNILAAVEATVPISLQVINVADQPQLVEYYRLVVTPALVKIGPGSRQVLSGIDLTDQLANQLPQWLVQQEAFFADREPPEVNIPFTELGQPETPALQQADAFFQLQQQYADLSERTKFLEQVIALVAHDLRNPLTAALLAVDTIQIRSQSFSVATAKEMQGLCSLFDQARSQLREIERMIAEILEATRHSGESLRINPREVVFEPLLQQVLEQLHERWRSKQQQLITDVPGDLPTLYADPDRLRQVLVNLLDNAIKYTPPGGTITIAALHRTSQKVQISISDTGSGIPRDQLSVIFKNLVRLSRDSSQEGYGIGLSVCQRIVQAHFGRIWVASELGQGSTFHFTMPVYRYTMPC 04A0A097C3EE0438 q9khi5_syne7 cikA Synpcc7942_0644 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MLAPSSNCSLASQRLTPEGFAQLQSALQDFVATLPQAFYWDSRSLHTHLRTQTGDCAIAIAAGFQLLLLGRTAAEYCQPHPLSEPHHVSVQFGADSIQRYCQATNLPVEYQPALAQLGDLSLNPDLISQFSNLLIAAIAADRAPLAAQYPAVSVCQPLEQALHWQEEQDRLISQVSAQIRLSLDLSEILTTTIREIRQLLNADRAIIYQFKPQCLDAGLDQRWPLYIPSQSYITYEDRRNEALLSVIDPLVQPGLLITTEEWQRFQQGETLLIDSVGFYKERLPEQYSFYERVQVRSVCKIPILVQGRIWGLLVAHQCQQDHRWQPRERDILQHLAEHLSIAIYQAQLYGQLQDQTQTLENRVLERTQELIDALALAQAANAAKGEFLATMSHELRTPLTCVIGMSSTLLRWAFGPLTERQREYIKAIHDSGEHLLELINDILDLSQIEAGKAALQVRPFSLSRLATQTLNTLQEKARLGEIQLMLDLQLNNRVDVFRADPKRLRQILINLLSNAVKFTEPQGTVFLRVWREGDRAIFQVSDTGIGIPESEQAQLFQKFQQLDTSIRRQYGGTGLGLALTKQLVELHGGHIQIESTVGQGSTFTVWIPEQTLIEPVEPRPSIDNLPAGHILLLEEEDEAATVVCEMLTAAGFKVIWLVDGSTALDQLDLLQPIVILMAWPPPDQSCLLLLQHLREHQADPHPPLVLFLGEPPVDPLLTAQASAILSKPLDPQLLLTTLQGLCPPNLSEGDRPSS F931DCB03F6FAF68 kaia_syne7 Circadian clock protein KaiA see0009 kaiA Synpcc7942_1218 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MLSQIAICIWVESTAILQDCQRALSADRYQLQVCESGEMLLEYAQTHRDQIDCLILVAANPSFRAVVQQLCFEGVVVPAIVVGDRDSEDPDEPAKEQLYHSAELHLGIHQLEQLPYQVDAALAEFLRLAPVETMADHIMLMGANHDPELSSQQRDLAQRLQERLGYLGVYYKRDPDRFLRNLPAYESQKLHQAMQTSYREIVLSYFSPNSNLNQSIDNFVNMAFFADVPVTKVVEIHMELMDEFAKKLRVEGRSEDILLDYRLTLIDVIAHLCEMYRRSIPRET DIP protein Q79PF6 original sequence version: 52 DIP protein Q79PF6 original sequence version: 58 DIP protein Q79PF6 original sequence version: 81 7C4F3B79FFDF59A2 q8gli4_syne7 ldpA Synpcc7942_0624 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MSASFPLEALQAGHWFKLICGASYQHLPVIRNLALTYALAGADCVDVAAEPAVVRSALAGLAAYERLTGRDRPWLMVSLNDGEDLHFRRAWFDPDRCPTDCPRPCERVCPTDAITSTGVQRDRCYGCGRCLPICPLGLIEAQAWQVDAAALLPELLDLGINAIEIHTQVGHQKEFQQLWQQLQPWLPQLQAIAISCPAHPEAIAYLWDLQELVGNTVETVIWQTDGRPMSGDIGAGTTHAAVRFAQAMLTEGPPGHVQLAGGTNAHTVAKLQELKLLAPQVSRFVAGIACGGAARTPLAELLEPLAEQQRSLEAHPEVLQAAVTTAIALVGPLKQAVGGATRSIPAFLLRTP 33614612158F2936 ldpa-cika-1 1 4 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association LdpA interacts with CikA independently of clock proteins as shown in kaiA, kaiB or kaiC mutant strains. LdpA interacts with KaiA independently of other clock proteins as shown in kaiB or kaiC mutant strains. ldpa-kaia-1 1 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association LdpA copurifies with circadian clock protein kaiA in a circadian fashion. KaiA is detected in pull-down 2 hours after the cyanbacteria are realeased in continuous light. LdpA interacts with KaiA independently of other clock proteins as shown in kaiB or kaiC mutant strains.