IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Biochemistry (0006-2960) 2005 Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom monoclonal western monoclonal antibody western blot monoclonal western Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. Biochemistry (0006-2960) 2005 Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Biochemistry (0006-2960) 2005 Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip monoclonal western monoclonal antibody western blot monoclonal western Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. Biochemistry (0006-2960) 2005 Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Biochemistry (0006-2960) 2005 Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. Biochemistry (0006-2960) 2005 Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation. Biochemistry (0006-2960) 2005 Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. imaging technique imaging technique microscopy immunostaining immunostaining Lan Z., Kurata WE., Martyn KD., Jin C., Lau AF. Confocal microscopy was used. Biochemistry (0006-2960) 2005 sgsm3_mouse Small G protein signaling modulator 3 Rutbc3 RUN and TBC1 domain-containing protein 3 Sgsm3 Cip85 protein protein mouse Mus musculus Mouse MSGNHTPSASGPFSALTPSIWPQEILAKYSQKEESSEQPELCYDEFGFRVDKEGSEPGCSQMTGSPLVEDPPQRLRWQAHLEFTHNHDVGDLTWDKIAVSLPRSEKLRSLVLAGIPHGMRPQLWMRLSGALQKKKNSELSYREIIKNSSNDETIAAKQIEKDLLRTMPSNACFANVNSIGVPRLRRVLRALAWLYPEIGYCQGTGMVAACLLLFLEEEDAFWMMCAIIEDLLPASYFSTTLLGVQTDQRVLRHLIVQYLPRLDKLLQEHDIELSLITLHWFLTAFASVVHIRLLLRIWDLFFYEGSLVLFQTTLGMLRLKEEELIQSENSASIFNTLSDIPAQMDDAELLLGEAMRLAGSLTDVAVETQRRKHLAYLIADQGQTLGTGTTTNLSQVVRRRTQRRKSGITSLLFGEDDLEALKAKNIKQTELVADLREAILRVARHFQCTDPKNCSVELTPDYSMESHQRDHENYVACLRSHRRRAKALLDFERHDDDELGFRKNDIITIISQKDEHCWVGELNGLRGWFPAKFVEVLDERSKEYSIAGDDSVTEGVTDLVRGTLCPALKALFEHGLKKPSLLGGACHPWLFIEEAAGREVERDFDSVYSRLVLCKTYRLDEDGKVLTPEELLYRAVQSVNVTHDAAHAQMDVKLRSLICVGLNEQVLHLWLEVLCSSLPTVEKWYQPWSFLRSPGWVQIKCELRVLCCFAFSLSQDWELPARREEEKQPLKEGVQDMLVKHHLFSWDIDG C0306BE346808B07 cxa1_rat Gap junction alpha-1 protein Gja1 Cxn-43 Connexin-43 Gap junction 43 kDa heart protein protein protein rat Rattus norvegicus (Rat) Rat MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI DIP protein P08050 original sequence version: 110 DIP protein P08050 original sequence version: 147 0196416EA6A69490 gja1-rutbc3-1 3 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait cytoplasmic region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association gja1-rutbc3-2 1 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3 domain binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association gja1-rutbc3-3 2 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey proline rich region binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P08050:p.[Pro253Ala;Pro256Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Fri Jul 22 11:03:48 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro253ala-pro256ala' Wed May 02 15:07:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro253ala,pro256ala' Tue Jun 30 18:35:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro253Ala;Pro256Ala]' physical association physical association gja1-rutbc3-4 4 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization