IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Hum. Mol. Genet. (0964-6906) 2004 Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu human-va13_2ra Homo sapiens Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) va13_2ra Human Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Hum. Mol. Genet. (0964-6906) 2004 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Hum. Mol. Genet. (0964-6906) 2004 Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Hum. Mol. Genet. (0964-6906) 2004 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Hum. Mol. Genet. (0964-6906) 2004 Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma fluorescence imaging fluorescence microscopy fluorescence imaging western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Hum. Mol. Genet. (0964-6906) 2004 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Hum. Mol. Genet. (0964-6906) 2004 Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Hum. Mol. Genet. (0964-6906) 2004 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Hum. Mol. Genet. (0964-6906) 2004 Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Hum. Mol. Genet. (0964-6906) 2004 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Hum. Mol. Genet. (0964-6906) 2004 Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu human-va13_2ra Homo sapiens Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) va13_2ra Human Caucasian fetal lung, SV40 transformed (WI 38VA13 Subline 2RA cell line) fluorescence imaging fluorescence microscopy fluorescence imaging western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Hum. Mol. Genet. (0964-6906) 2004 Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Association and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2. Hum. Mol. Genet. (0964-6906) 2004 Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lillard-Wetherell K., Machwe A., Langland GT., Combs KA., Behbehani GK., Schonberg SA., German J., Turchi JJ., Orren DK., Groden J. nuclear extracts from human Saos2 cells were used. fig 2D joanna.groden@uc.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Hum. Mol. Genet. (0964-6906) 2004 blm_human Bloom syndrome protein RecQ protein-like 3 DNA helicase, RecQ-like type 2 BLM RECQ2 RECQL3 protein protein human Homo sapiens Human MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS DIP protein P54132 original sequence version: 155 DIP protein P54132 original sequence version: 109 DIP protein P54132 original sequence version: 146 DIP protein P54132 original sequence version: 111 423DF5F381194E11 terf1_human Telomeric repeat-binding factor 1 TTAGGG repeat-binding factor 1 NIMA-interacting protein 2 Telomeric protein Pin2/TRF1 TERF1 PIN2 TRF TRF1 TRBF1 protein protein human Homo sapiens Human MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED DIP protein P54274 original sequence version: 121 DIP protein P54274-1 original sequence version: 81 DIP protein P54274 original sequence version: 168 AB548E7D3124A211 terf2_human Telomeric repeat-binding factor 2 TTAGGG repeat-binding factor 2 Telomeric DNA-binding protein TERF2 TRF2 TRBF2 protein protein human Homo sapiens Human MAAGAGTAGPASGPGVVRDPAASQPRKRPGREGGEGARRSDTMAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAGEARLEEAVNRWVLKFYFHEALRAFRGSRYGDFRQIRDIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNIIREKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTMAKKALKSESAASSTGKEDKQPAPGPVEKPPREPARQLRNPPTTIGMMTLKAAFKTLSGAQDSEAAFAKLDQKDLVLPTQALPASPALKNKRPRKDENESSAPADGEGGSELQPKNKRMTISRLVLEEDSQSTEPSAGLNSSQEAASAPPSKPTVLNQPLPGEKNPKVPKGKWNSSNGVEEKETWVEEDELFQVQAAPDEDSTTNITKKQKWTVEESEWVKAGVQKYGEGNWAAISKNYPFVNRTAVMIKDRWRTMKRLGMN DIP protein Q15554 original sequence version: 107 DIP protein Q15554 original sequence version: 109 DIP protein Q15554 original sequence version: 121 DIP protein Q15554 original sequence version: 157 DIP protein Q15554-3 original sequence version: 115 3A278AC6B594C43A blm-terf2-1 4 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association blm-terf2-2 1 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association blm-terf2-4 3 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization BLM and TRF2 co-localize with foci of DNA synthesis Co-localization is enriched in late S amd G2/M phases. BLM and TRF2 co-localize in telomerase-negative immortalized cells using ALT (Saos2, VA13/2R) but does not colocalize in telomerase-positive cells (MCF7) and primary cells (WI-38). blm-terf2-5 6 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization BLM and TRF2 co-localize with foci of DNA synthesis Co-localization is enriched in late S amd G2/M phases. BLM and TRF2 co-localize in telomerase-negative immortalized cells using ALT (Saos2, VA13/2R) but does not colocalize in telomerase-positive cells (MCF7) and primary cells (WI-38). blm-terf1-6 5 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. direct interaction direct interaction blm-terf1-7 2 8 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction blm-terf2-6 7 8 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction blm-terf1-8 2 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined helicase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction blm-terf2-9 2 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined helicase domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 10 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction blm-terf2-10 2 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 10 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction