IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Subunit composition of NDH-1 complexes of Synechocystis sp. PCC 6803: identification of two new ndh gene products with nuclear-encoded homologues in the chloroplast Ndh complex. Subunit composition of NDH-1 complexes of Synechocystis sp. PCC 6803: identification of two new ndh gene products with nuclear-encoded homologues in the chloroplast Ndh complex. J. Biol. Chem. (0021-9258) 2004 Prommeenate P., Lennon AM., Markert C., Hippler M., Nixon PJ. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species NDH-1 complex was purified from Synechocystis sp. PCC 6803 in which NDhJ subunit was His-tagged. Pull-downed proteins were subjected to SDS-PAGE and coomassie blue staining and identified by N-terminal sequencing and mass spectrometry. The complex could be further fragmented in sub-complexes by pre-treatment with Triton X-100. syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) biochemical biochemical protein sequence protein sequence identification protein sequence Prommeenate P., Lennon AM., Markert C., Hippler M., Nixon PJ. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species NDH-1 complex was purified from Synechocystis sp. PCC 6803 in which NDhJ subunit was His-tagged. Pull-downed proteins were subjected to SDS-PAGE and coomassie blue staining and identified by N-terminal sequencing and mass spectrometry. The complex could be further fragmented in sub-complexes by pre-treatment with Triton X-100. J. Biol. Chem. (0021-9258) 2004 nu2c_syny3 NAD(P)H-quinone oxidoreductase subunit 2 NAD(P)H dehydrogenase subunit 2 NADH-plastoquinone oxidoreductase subunit 2 ndhB sll0223 NDH-1, subunit 2 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MDFSSNVAAQLNAGTILPEGIVIVTLLLVLIVDLIGGRKVALALPYLAIAGLLVSVGLLVTSWSMADPIGFIGAFNGDNLSIIFRAIIALSTVVTILMSVRYVQQTGTSLAEFIAILLTATLGGMFLSAANELVMVFISLEMLSISSYLMTGYMKRDPRSNEAALKYLLIGASSSAIFLYGLSLLYGLSGGETQLVLIAEKLVNADTVGQSLGLAIALVFVIAGIAFKISAVPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFAVAIRLLVTAFGGITDEWHVIFTALAVLSMVLGNVVALAQTSMKRMLAYSSIGQAGFVMIGLVAGSEDGYASMVFYMLIYLFMNLGAFSCIILFTLRTGSDQISDYAGLYHKDPLLTLGLSICLLSLGGIPPLAGFFGKIYIFWAGWQSGLYGLVLLGLVTSVVSIYYYIRVVKMMVVKEPQEMSEVIKNYPAIKWNLPGMRPLQVGIVATLVATSLAGILANPLFNLATDSVVSTKMLQTALQQTGETPAIAISHDLP C18C06704C277081 ndhh_syny3 NAD(P)H-quinone oxidoreductase subunit H NAD(P)H dehydrogenase subunit H NADH-plastoquinone oxidoreductase subunit H ndhH slr0261 NDH-1 subunit H protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MTKIETRTEPMVLNMGPHHPSMHGVLRLIVTLDGEDVVDCEPVIGYLHRGMEKIAESRTNIMYVPYVSRWDYAAGMFNEAITVNAPEKLADIEVPKRAQYIRVIMLELNRIANHLLWLGPFMADVGAQTPFFYIFREREMIYDLWEAASGMRLINNNYFRVGGVAVDLPYGWNDKCEDFCDYFLPKVDEYEKLITNNPIFRRRVEGVGTVTREEAINWGLSGPMLRGSGVKWDLRKVDHYECYDELDWEVQYETAGDCFARYLVRIREMRESVKIIRQALKAMPGGPYENLEAKRLQEGKKSEWNDFQYQYIAKKVAPTFKIPAGEHYVRLESGKGELGIFIQGNDDVFPWRWKIRSADFNNLQILPHILKGVKVADIMAILGSIDIIMGSVDR C75A5CBDB502391F ndhj_syny3 NAD(P)H-quinone oxidoreductase subunit J NAD(P)H dehydrogenase subunit J NADH-plastoquinone oxidoreductase subunit J ORF 155 ndhJ slr1281 NDH-1 subunit J protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MAEEVNSPNEAVNLQEETAIAPVGPVSTWLTTNGFEHQSLTADHLGVEMVQVEADLLLPLCTALYAYGFNYLQCQGAYDEGPGKSLVSFYHLVKLTEDTRNPEEVRLKVFLPRENPVVPSVYWIWKAADWQERECYDMFGIVYEGHPNLKRILMPEDWVGWPLRKDYISPDFYELQDAY 3EE18D5E727F8BCC nu3c_syny3 NAD(P)H-quinone oxidoreductase subunit 3 NAD(P)H dehydrogenase subunit 3 NADH-plastoquinone oxidoreductase subunit 3 ndhC slr1279 NDH-1 subunit 3 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MFVLTGYEYFLGFLFICSLVPVLALTASKLLRPRDGGPERQTTYESGMEPIGGAWIQFNIRYYMFALVFVVFDVETVFLYPWAVAFNQLGLLAFVEALIFIAILVVALVYAWRKGALEWS 39343E8AFEAD8184 ndhi_syny3 NAD(P)H-quinone oxidoreductase subunit I NAD(P)H dehydrogenase I subunit I NDH-1 subunit I ndhI sll0520 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MFNNILKQVGDYAKESLQAAKYIGQGLAVTFDHMSRRPITVQYPYEKLIPSERFRGRIHFEFDKCIACEVCVRVCPINLPVVDWEFNKAVKKKELKHYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELAAYDRHDLNYDNVALGRLPYKVTEDPMVTPLRELGYLPKGVIEPHNLPKGSQRAGQHPEDLVKAE C67389694E391B0E ndhm_syny3 NAD(P)H-quinone oxidoreductase subunit M slr1623 ndhM NAD(P)H dehydrogenase I subunit M protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MLVKSTTRHVRIFSAEVQGNELIPSNNVLTMDVDPDNEFVWNEDALQQVYRRFDELVESYSGEDLTDYNLRRIGSDLEHFIRDLLQAGKVSYNLDCRVLNYSMGLPKVENQETAGKYWLDN 857E12852DE8656A ndhn_syny3 NAD(P)H-quinone oxidoreductase subunit N sll1262 ndhN NAD(P)H dehydrogenase I subunit N protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MLPLPLIANGKGFIRALENDGALAVYAPLEGGYEGRYQRRLRANGYASISLSARGLGDVEAYLMQVHGVRPAHLGKKNIAQEGAVGPIYFAQPIAGYQLENLPAQSKGLVLWILEGYILSQTEIQDLISLTKRVPKLKVVLEMGGDRVFRWQPLLDCLQAA 92E8953D18192973 ndhk1_syny3 NAD(P)H-quinone oxidoreductase subunit K 1 ndhK1 NAD(P)H dehydrogenase I subunit K 1 NDH-1 subunit K 1 psbG slr1280 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MSPNPANPTDLERVATAKILNPASRSQVTQDLSENVILTTVDDLYNWAKLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGLVPRSSPRQADLIITAGTITMKMAPALVRLYEEMPEPKYVIAMGACTITGGMFSSDSTTAVRGVDKLIPVDVYIPGCPPRPEAIFDAIIKLRKKVANESIQERAITQQTHRYYSTSHQMKVVAPILDGKYLQQGTRSAPPRELQEAMGMPVPPALTTSQQKEQLNRG FF68BEA64B92F6E7 nu5c_syny3 NAD(P)H-quinone oxidoreductase chain 5 NAD(P)H dehydrogenase I, chain 5 NDH-1, chain 5 ndhF slr0844 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MELLYQLAWLIPVLPLFGATVVGIGLISFNQATNKLRQINAVFIISCLGAALVMSGALLWDQIQGHASYAQMIEWASAGSFHLEMGYVIDHLSALMLVIVTSVALLVMIYTDGYMAHDPGYVRFYAYLSLFASSMLGLVISPNLVQVYIFWELVGMCSYLLIGFWYDRKAAADACQKAFVTNRVGDFGLLLGILGLYWATGSFDFGTIGERLEGLVSSGVLSGAIAAILAILVFLGPVAKSAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARMYPVFEPIPVVMNTIAFTGCFTAFLGATIALTQNDIKKGLAYSTISQLGYMVMAMGIGAYSAGLFHLMTHAYFKAMLFLCSGSVIHGMEGVVGHDPILAQDMRIMGGLRKYMPITATCFLIGTLAICGIPPFAGFWSKDEILGLAFQANPLLWFVGWATAGMTAFYMFRMYFMTFEGGFRGNDQEAKDGVLQFYGLLPNFGPGAMNVKELDHEAGHDDHGHSSEPHESPLTMTFPLMALAVPSVLIGLLGRPWANQFEAFIHAPGEVVEHAAEFEWGEFYVMAGNSIGIALIGITVASLMYWQHKFDPKVLAEKFPSLYQLSLNKWYFDDLYDKLFVQGSRRVARQIMEVDYKVIDGAVNLTGLVTLVSGEGLKYLENGRAQFYALIVFGAVLGFVIVFSLT D110C883B722FDAE nu1c_syny3 NAD(P)H-quinone oxidoreductase subunit 1 NAD(P)H dehydrogenase I subunit 1 NDH-1 subunit 1 NDH-A ndhA sll0519 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MTSGIDLQNSFLQSLQGFGLPPGLAKLFWIPLPSILMIIGATVGVLVVVWLERKISAAAQQRIGPEYAGPLGVLQPVADGIKLVFKEDVVPAKADPWLFTLGPVLVVLPVFLSYLIVPFGQNLVITDINVGIFLWIALSSIAPIGLLMSGYASNNKYSLLGGLRAAAQSISYEIPLSLAVLAIVMMSNSLSTIDIVDQQSGYGILGWNIWRQPVGFLIFWIAALAECERLPFDLPEAEEELVAGYQTEYAGMKFALFYLGSYVNLVLSALVFSVLYLGGWDFPIPLENVANWLGVAPTTSWLQVLMAALGITMTVLKSYFLIFIAILLRWTVPRVRIDQLLNLGWKFLLPVALANLLITAALKLTFPMAFGG 4D9E02576825E990 nu4c1_syny3 NAD(P)H-quinone oxidoreductase chain 4-1 NAD(P)H dehydrogenase I, subunit D-1 NDH-1, chain 4-1 ndhD1 ndhD slr0331 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MNTFPWLTTIILLPIVAALFIPIIPDKDGKTVRWYSLAVGLVDFALIVYAFYSGFDLSEPGLQLVESYTWLPQIDLKWSVGADGLSMPLIILTGFITTLATMAAWPVTLKPKLFYFLMLLMYGGQIAVFAVQDILLFFLVWELELVPVYLILSIWGGKKRLYAATKFILYTAGGSLFILLAGLTLAFYGDVNTFDMSAIAAKDIPVNLQLLLYAGFLIAYGVKLPIFPLHTWLPDAHGEATAPAHMLLAGILLKMGGYALLRMNVGMLPDAHAVFAPVLVILGVVNIIYAAFTSFAQRNLKRKIAYSSISHMGFVLIGLASFTDLGMSGAMLQMISHGLIGASLFFMVGATYDRTHTLMLDEMGGIGQKMKKGFAMWTACSLASLALPGMSGFVAELMVFVGFATSDAYNLVFRTIVVVLMGVGVILTPIYLLSMLREMLYGPENEELVNHTNLVDVEPREVFIIGCLLVPIIGIGFYPKLITQIYDPTINQLVQTARRSVPSLVQQANLSPLEVTALRPPTIGF CC478354466E7DF9 nu6c_syny3 NAD(P)H-quinone oxidoreductase chain 6 NAD(P)H dehydrogenase I, chain 6 NDH-1, chain 6 ndhG sll0521 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MNLAEGVQYISFLILAFLVIGAALGVVLLSNIVYSAFLLGGVFLSISGIYILLNADFVAAAQVLVYVGAVSVLILFAIMLVNKREDFSKIPGRWLRNVSTALVCTGIFALLSTMVLITPWQINETGPFVENTLVTIGKHFFSDYLLPFELASVLLLMAMVGAIILARRDLIPELSEENKTATALTLPERPRELTSASK A92685FB148C5FEE ndhj-ndhi-1 1 13 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component physical association physical association ndhj-ndhi-2 1 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey short fragment binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association all stoichiometry is 1 ndhj-ndhh-3 1 4 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey physical association physical association all stoichiometry is 1 ndhj-ndhi-4 1 5 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 2 unspecified role unspecified role neutral component neutral component physical association physical association ndhj-ndhh-1 1 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey physical association physical association all stoichiometry is 1 ndhj-ndhi-5 1 8 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey short fragment binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association