IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain. The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain. J. Cell. Sci. (0021-9533) 2004 Finnerty CM., Chambers D., Ingraffea J., Faber HR., Karplus PA., Bretscher A. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight by comassie molecular weight estimation by coomasie staining weight by comassie Finnerty CM., Chambers D., Ingraffea J., Faber HR., Karplus PA., Bretscher A. The purified bait was coupled to cyanogen-activated Sepharose beads. A cell lysate expressing the prey was then added. After washes, analysis was done by SDS-PAGE and coomassie-blue staining. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Cell. Sci. (0021-9533) 2004 The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain. The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain. J. Cell. Sci. (0021-9533) 2004 Finnerty CM., Chambers D., Ingraffea J., Faber HR., Karplus PA., Bretscher A. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Finnerty CM., Chambers D., Ingraffea J., Faber HR., Karplus PA., Bretscher A. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Cell. Sci. (0021-9533) 2004 ezri_human Ezrin EZR VIL2 p81 Cytovillin Villin-2 protein protein human Homo sapiens Human MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL DIP protein P15311 original sequence version: 170 DIP protein P15311 original sequence version: 142 F1B592CF49A7CC46 moes_human Moesin Membrane-organizing extension spike protein MSN protein protein human Homo sapiens Human MPKTISVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWFFGLQYQDTKGFSTWLKLNKKVTAQDVRKESPLLFKFRAKFYPEDVSEELIQDITQRLFFLQVKEGILNDDIYCPPETAVLLASYAVQSKYGDFNKEVHKSGYLAGDKLLPQRVLEQHKLNKDQWEERIQVWHEEHRGMLREDAVLEYLKIAQDLEMYGVNYFSIKNKKGSELWLGVDALGLNIYEQNDRLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQMERAMLENEKKKREMAEKEKEKIEREKEELMERLKQIEEQTKKAQQELEEQTRRALELEQERKRAQSEAEKLAKERQEAEEAKEALLQASRDQKKTQEQLALEMAELTARISQLEMARQKKESEAVEWQQKAQMVQEDLEKTRAELKTAMSTPHVAEPAENEQDEQDENGAEASADLRADAMAKDRSEEERTTEAEKNERVQKHLKALTSELANARDESKKTANDMIHAENMRLGRDKYKTLRQIRQGNTKQRIDEFESM DIP protein P26038 original sequence version: 168 865A6C5CB14AE586 nhrf1_human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 SLC9A3R1 NHERF NHERF1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 protein protein human Homo sapiens Human MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL E33AF87016D37A65 msn-nherf-1 2 4 unspecified role unspecified role neutral component neutral component ferm domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component c-terminus binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-528787]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 359-359, sequence length 358 [EBI-528787]322-359 in vitro In vitro direct interaction direct interaction 3.50 working 33.8%, free 40.1% msn-nherf-2 1 4 unspecified role unspecified role bait bait ferm domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-528799]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 359-359, sequence length 358 [EBI-528799]322-359 O14745:p.Phe355Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F R Wed May 02 16:13:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe355arg' Tue Jun 30 16:35:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe355Arg' delta leu358 binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-528803]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 359-359, sequence length 358 [EBI-528803]339-359 mbp tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association msn-nherf-3 1 3 unspecified role unspecified role neutral component neutral component ferm domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P15311:p.[Asn210Phe;Thr214Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N F certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:29:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn210phe,thr214ala' Tue Jun 30 18:53:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn210Phe;Thr214Ala]' ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component c-terminus binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-528810]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 359-359, sequence length 358 [EBI-528810]322-359 in vitro In vitro physical association physical association