IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol. Biol. Cell (1059-1524) 2004 Eugster A., Frigerio G., Dale M., Duden R. Superose 6 gel filtration of cytosolic proteins: purification of COPI complex (670 kDa) fig 4d rd217@cam.ac.uk yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Eugster A., Frigerio G., Dale M., Duden R. Superose 6 gel filtration of cytosolic proteins: purification of COPI complex (670 kDa) fig 4d rd217@cam.ac.uk Mol. Biol. Cell (1059-1524) 2004 The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol. Biol. Cell (1059-1524) 2004 Eugster A., Frigerio G., Dale M., Duden R. Superose 6 gel filtration of cytosolic proteins: purification of COPI complex (670 kDa) fig 4d rd217@cam.ac.uk yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Eugster A., Frigerio G., Dale M., Duden R. Superose 6 gel filtration of cytosolic proteins: purification of COPI complex (670 kDa) fig 4d rd217@cam.ac.uk Mol. Biol. Cell (1059-1524) 2004 The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol. Biol. Cell (1059-1524) 2004 Eugster A., Frigerio G., Dale M., Duden R. Superose 6 gel filtration of cytosolic proteins: purification of COPI complex (670 kDa) fig 4d rd217@cam.ac.uk in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Eugster A., Frigerio G., Dale M., Duden R. Superose 6 gel filtration of cytosolic proteins: purification of COPI complex (670 kDa) fig 4d rd217@cam.ac.uk Mol. Biol. Cell (1059-1524) 2004 copd_yeast Coatomer subunit delta RET2 YFR051C Delta-coat protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVVLAASITTRQGKPLLSRQFKDLSKDRVLELLSNFQNLVSEISSDHTFVEDKHVRYVYRPFDNYYIILITNRQSNIIKDLATLNLFSQTINSYLSSFQDQEIFHNAFEILSSFDEIVSMGGYKENLSFTQVQTYLSMESHEERIQEIIERNKEIEATEERKRRAKEIARKEHERKHGFMSSNGDYDGANRFMGSKDPNVTNAINSYYSHASPAAQQSYLQSSHAAAAEVAPVASPMATSQRAGHSATGGMKLGGGAGRRAGAAPRPSAISSASSGTPPPPEEDVPENNGILISIKEVINAEFSRDGTIHSSELKGVLELRINDHDLSHSNLKLADSIDVRDKSFQFKTHPNIDKQSFLSTKLISLRDKSKAFPANDQSLGVLRWRKVAPAEDDSLIPLTLTTWVSPSESQQGFDVIIEYESVLETELADVIFTIPVFPQEPVDINTESSTCSDAEVVNMDQEMGTSIKISKIAANDAGALAFTIEAPYEDALYPMTVSFQESTRDKLAKSFTGMAIQSVVMANDHDQELPYDVITSLKSDEYLVQ AA730F046655EE5A AA730F046655EE5A ostb_yeast Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 WBP1 Oligosaccharyl transferase subunit beta YEL002C protein protein yeast Saccharomyces cerevisiae Baker's yeast MRTDWNFFFCILLQAIFVVGTQTSRTLVLYDQSTEPLEEYSVYLKDLEQRNYKLEYLDINSTSTTVDLYDKEQRLFDNIIVFPTKGGKNLARQIPVKQLIKFFENEGNILCMSSPGAVPNTIRLFLNELGIYPSPKGHVIRDYFSPSSEELVVSSNHLLNKYVYNARKSEDFVFGESSAALLENREQIVPILNAPRTSFTESKGKCNSWTSGSQGFLVVGFQNLNNARLVWIGSSDFLKNKNQDSNQEFAKELLKWTFNEKSVIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGEEWLPHIADDIQFELRQVDPYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIGLSFTTDKDVKAIRHLANDEYPRSWEISNSWVYISAICGVIVAWIFFVVSFVTTSSVGKKLETFKKTN 495DA76BE8A9B29A 495DA76BE8A9B29A emp47_yeast Protein EMP47 YFL048C EMP47 47 kDa endomembrane protein Endosomal P44 protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MMMLITMKSTVLLSVFTVLATWAGLLEAHPLGDTSDASKLSSDYSLPDLINARKVPNNWQTGEQASLEEGRIVLTSKQNSKGSLWLKQGFDLKDSFTMEWTFRSVGYSGQTDGGISFWFVQDSNVPRDKQLYNGPVNYDGLQLLVDNNGPLGPTLRGQLNDGQKPVDKTKIYDQSFASCLMGYQDSSVPSTIRVTYDLEDDNLLKVQVDNKVCFQTRKVRFPSGSYRIGVTAQNGAVNNNAESFEIFKMQFFNGVIEDSLIPNVNAMGQPKLITKYIDQQTGKEKLIEKTAFDADKDKITNYELYKKLDRVEGKILANDINALETKLNDVIKVQQELLSFMTTITKQLSSKPPANNEKGTSTDDAIAEDKENFKDFLSINQKLEKVLVEQEKYREATKRHGQDGPQVDEIARKLMIWLLPLIFIMLVMAYYTFRIRQEIIKTKLL 3978462BC2AFCD8A cope_yeast Coatomer subunit epsilon SEC28 YIL076W Epsilon-coat protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYFNIKQNYYTGNFVQCLQEIEKFSKVTDNTLLFYKAKTLLALGQYQSQDPTSKLGKVLDLYVQFLDTKNIEELENLLKDKQNSPYELYLLATAQAILGDLDKSLETCVEGIDNDEAEGTTELLLLAIEVALLNNNVSTASTIFDNYTNAIEDTVSGDNEMILNLAESYIKFATNKETATSNFYYYEELSQTFPTWKTQLGLLNLHLQQRNIAEAQGIVELLLSDYYSVEQKENAVLYKPTFLANQITLALMQGLDTEDLTNQLVKLDHEHAFIKHHQEIDAKFDELVRKYDTSN 95ACA8BB647817DB 95ACA8BB647817DB copb2_yeast Coatomer subunit beta' SEC27 YGL137W G2827 Beta'-coat protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLDIKKTFSNRSDRVKGIDFHPTEPWVLTTLYSGRVELWNYETQVEVRSIQVTETPVRAGKFIARKNWIIVGSDDFRIRVFNYNTGEKVVDFEAHPDYIRSIAVHPTKPYVLSGSDDLTVKLWNWENNWALEQTFEGHEHFVMCVAFNPKDPSTFASGCLDRTVKVWSLGQSTPNFTLTTGQERGVNYVDYYPLPDKPYMITASDDLTIKIWDYQTKSCVATLEGHMSNVSFAVFHPTLPIIISGSEDGTLKIWNSSTYKVEKTLNVGLERSWCIATHPTGRKNYIASGFDNGFTVLSLGNDEPTLSLDPVGKLVWSGGKNAAASDIFTAVIRGNEEVEQDEPLSLQTKELGSVDVFPQSLAHSPNGRFVTVVGDGEYVIYTALAWRNKAFGKCQDFVWGPDSNSYALIDETGQIKYYKNFKEVTSWSVPMHSAIDRLFSGALLGVKSDGFVYFFDWDNGTLVRRIDVNAKDVIWSDNGELVMIVNTNSNGDEASGYTLLFNKDAYLEAANNGNIDDSEGVDEAFDVLYELSESITSGKWVGDVFIFTTATNRLNYFVGGKTYNLAHYTKEMYLLGYLARDNKVYLADREVHVYGYEISLEVLEFQTLTLRGEIEEAIENVLPNVEGKDSLTKIARFLEGQEYYEEALNISPDQDQKFELALKVGQLTLARDLLTDESAEMKWRALGDASLQRFNFKLAVEAFTNAHDLESLFLLHSSFNNKEGLVTLAKDAERAGKFNLAFNAYWIAGDIQGAKDLLIKSQRFSEAAFLGSTYGLGDDAVNDIVTKWKENLILNGKNTVSERVCGAEGLPGSSSSGDAQPLIDLDSTPAPEQADENKEAEVEDSEFKESNSEAVEAEKKEEEAPQQQQSEQQPEQGEAVPEPVEEES 6A5E50BBEB02CB58 6A5E50BBEB02CB58 copb_yeast Coatomer subunit beta SEC26 YDR238C YD8419.05C Beta-coat protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSLSSQPAYTLVFDPSPSMETYSSTDFQKALEKGSDEQKIDTMKSILVTMLEGNPMPELLMHIIRFVMPSKNKELKKLLYFYWEIVPKLAEDGKLRHEMILVCNAIQHDLQHPNEYIRGNTLRFLTKLREAELLEQMVPSVLACLEYRHAYVRKYAILAVFSIFKVSEHLLPDAKEIINSFIVAETDPICKRNAFIGLAELDRENALHYLENNIADIENLDPLLQAVFVQFIRQDANRTPALKAQYIELLMELLSTTTSDEVIFETALALTVLSANPNVLVPAVNKLIDLAVKVSDNNIKLIVLDRIQDINANNVGALEELTLDILRVLNAEDLDVRSKALDISMDLATSRNAEDVVQLLKKELQTTVNNPDQDKAMQYRQLLIKTIRTVAVNFVEMAASVVSLLLDFIGDLNSVAASGIIAFIKEVIEKYPQLRANILENMVQTLDKVRSAKAYRGALWIMGEYAEGESEIQHCWKHIRNSVGEVPILQSEIKKLTQNQEHTEENEVDATAKPTGPVILPDGTYATESAFDVKTSQKSVTDEERDSRPPIRRFVLSGDFYTAAILANTIIKLVLKFENVSKNKTVINALKAEALLILVSIVRVGQSSLVEKKIDEDSLERVMTSISILLDEVNPEEKKEEVKLLEVAFLDTTKSSFKRQIEIAKKNKHKRALKDSCKNIEPIDTPISFRQFAGVDSTNVQKDSIEEDLQLAMKGDAIHATSSSSISKLKKIVPLCGFSDPVYAEACITNNQFDVVLDVLLVNQTKETLKNLHVQFATLGDLKIIDTPQKTNVIPHGFHKFTVTVKVSSADTGVIFGNIIYDGAHGEDARYVILNDVHVDIMDYIKPATADDEHFRTMWNAFEWENKISVKSQLPTLHAYLRELVKGTNMGILTPSESLGEDDCRFLSCNLYAKSSFGEDALANLCIEKDSKTNDVIGYVRIRSKGQGLALSLGDRVALIAKKTNKLALTHV 885420DB026BCFA3 885420DB026BCFA3 copa_yeast Coatomer subunit alpha COP1 RET1 SEC33 YDL145C D1578 SOO1 Alpha-coat protein Retrieval from endoplasmic reticulum protein 1 Secretory protein 22 Suppressor of osmo-sensitivity 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKMLTKFESKSTRAKGIAFHPSRPWVLVALFSSTIQLWDYRMGTLLHRFEDHEGPVRGLDFHPTQPIFVSAGDDYTIKVWSLDTNKCLYTLTGHLDYVRTVFFHRELPWIISASDDQTIRIWNWQNRKEIACLTGHNHFVMCAQFHPTDDLIVSASLDETIRIWDISGLRKRHSAPGTSSFEEQMSAQQNLLDGSLGDCVVKFILEGHTRGVNWASFHPTLPLIVSGSDDRQVKLWRMSATKAWEVDTCRGHTNNVDSVIFHPHQNLIISVGEDKTLRVWDLDKRTPVKQFKRENDRFWLIAAHPHINLFGAAHDSGIMVFKLDRERPCSFIHQNQLFFVNAEKQIQSFNFQKRVASLPYASLKGIGQPWDAFRSISYNPSQHSVLVNEANGKFALVILPKQPVGAVEPTSVTQDTGNFATFVGRNRFVVYNKNTESVEVRSLENKVTRNIKVEETVRTIVAAGPGSVLVIHPREVILYDVQQGKKVSQLAVKNVKYVSWSLDGQYVALMSKHTITLATKKLELINSMHETIRIKSAAWDETGVLIYSTLNHIRYSLLNGDRGIIKTLEKTLYITKVQGKLVYCLNREGEIEILTIDPTEYRFKKALVNKNFPEVLRLIKDSNLVGQNIISYLQKSGYPEIALQFVQDPHIRFDLALEYGNLDVALDEAKKLNDSSTWERLIQEALAQGNASLAEMIYQTQHSFDKLSFLYLVTGDVNKLSKMQNIAQTREDFGSMLLNTFYNNSTKERSSIFAEGGSLPLAYAVAKANGDEAAASAFLEQAEVDEQDVTLPDQMDASNFVQRPVISKPLEKWPLKEAELSYFEKAVLGQIDDLTIDDETPAVNTTQEQEEPLGEENFNDEDIGEDEGAWDLGDEDLDVGEELPEEVEQGEITSPAQEVETAIWIKNSKLPAVLVAAGAFDAAVQALSKQVGVVKLEPLKKYFTNIYEGCRTYIPSTPCELPAQLGYVRAYDDTVSEDQILPYVPGLDVVNEKMNEGYKNFKLNKPDIAIECFREAIYRITLLMVDDAEDEKLAHKILETAREYILGLSIELERRSLKEGNTVRMLELAAYFTKAKLSPIHRTNALQVAMSQHFKHKNFLQASYFAGEFLKIISSGPRAEQARKIKNKADSMASDAIPIDFDPYAKFDICAATYKPIYEDTPSVSDPLTGSKYVITEKDKIDRIAMISKIGAPASGLRIRV D3589073106F5570 D3589073106F5570 copg_yeast Coatomer subunit gamma SEC21 YNL287W N0543 Gamma-coat protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAHTYKKFENSTSGDLPDKMTIYQDCMNTFNESPVNSKRCRLLISRLLRLLAQGETFPQNEATALFFSISKLFQHQNDPLRQAVYLAIKELSGISEDVLMATSSIMKDVQNGSDLIKPDAIRSLTYVLDESTAFSAERLLKSAVVSRHPSISSAALCTSYHLLPISEVTIRRFTNETQEAVLDLKQFPNQHGNSEYYPNSTYISQYHALGLLYQLKKTDKMALLKLVRHFSENNSMKNQLAKVELVKIVNDLIYRDPQLFSQFRPLLSDWLSNKFESVQLETAKLITSFATRNSRLVAPELYAAAISALQSLLTVPRVSSRFAALRILNRISMVSPEKIVVCNPELESLINDSNRNISTYAITTLLKTGTSKNISSLISTITNFIHDVSDDFKIIIIDAVRTLSLNFPQEWKSILNFLIDVLKNSEGGFKFKNSIVEALIDIVSFVPQSKELALENLCDFIEDCEFNEILVRILHLLGKEGPSAPNPSLYVRHIYNRVVLENSIIRSAAVVALSKFALTKNDPTLYESIISLLKRIANDKDDEVRDRATIALEFIDSARNKDDVIAQNLIESKYFYDIPSLESKLSSYISSNTDSFATAFDVNQVRKFTEDEMKAINLKRKQEQIFNQKSETTLDTTPEAESVPEKRADANSFAGPNLDDHQEDLLATKYADELLSIEQIKPFGQLVNSSRAISLTEPEAEFVVRGVKHLFKDNVVLQFNITNTLTDIALDNVSVVCTPEISDEAELEELFTLQVDRLLPSEEAACYVAFKKLDEIVMEGFLNNLTFTTKEINPDTNEPFDGDEGFQDEYEIDSIFLNAGDYVKSSFTGNFSATFDELPCEEVAVFNIQEDLSIQEVVDKIILNSSCLPVESTQFAPSDSNSHTLKLFGKSALTGSKVALQIKMIKSSKGLALKVHGKGEDSLLCSDLVNGLMQ 99DC7D737D4EF761 99DC7D737D4EF761 ret1-wbp1-1 2 5 unspecified role unspecified role bait bait di-lysine motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-619799]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 431-431, sequence length 430 [EBI-619799]416-431 P33767:p.[Lys427Ala;Lys428Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:11:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys427ala,lys428ala' Tue Jun 30 16:29:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys427Ala;Lys428Ala]' 10 unspecified role unspecified role prey prey physical association physical association ret1-sec27-1 2 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association sec27-emp47-1 2 6 unspecified role unspecified role bait bait di-lysine motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-619861]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 446-446, sequence length 445 [EBI-619861]436-446 P43555:p.[Lys441Ser;Lys443Ser] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain K S Wed May 02 19:31:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys441ser,lys443ser' Tue Jun 30 18:27:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys441Ser;Lys443Ser]' 8 unspecified role unspecified role prey prey wd40 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association ret1-wbp1-2 3 10 unspecified role unspecified role prey prey P53622:p.Gly227Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G E Wed May 02 19:20:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly227glu' Tue Jun 30 19:38:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly227Glu' wd40 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait di-lysine motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-619987]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 431-431, sequence length 430 [EBI-619987]416-431 P33767:p.[Lys427Ser;Lys428Ser] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain K S Wed May 02 20:24:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys427ser,lys428ser' Tue Jun 30 17:24:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys427Ser;Lys428Ser]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association Deletion of WD40 domain or Ser114Tyr mutation of COP beta 2 impairs COP alpha and epsilon pull-down with the KKTN motif ret1-emp47-1 3 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined di-lysine motif binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-620038]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 446-446, sequence length 445 [EBI-620038]436-446 P43555:p.[Lys441Ser;Lys443Ser] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K S certain certain sequence position certain certain certain sequence position certain K S Wed May 02 17:21:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys441ser,lys443ser' Tue Jun 30 20:07:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys441Ser;Lys443Ser]' ecobb Escherichia coli (strain B / BL21) 10 unspecified role unspecified role prey prey wd40 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association WD40 domain of COP beta 2 is necessary for COP alpha and epsilon interaction with the KTKLL motif ret1-sec28-1 2 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association sec27-sec28-2 2 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association copa-cope-5 1 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association