IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-mnt-1 Homo sapiens melanoma cell line mnt-1 MNT human melanoma cells density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-mnt-1 Homo sapiens melanoma cell line mnt-1 MNT human melanoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-mnt-1 Homo sapiens melanoma cell line mnt-1 MNT human melanoma cells density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J. Biol. Chem. (0021-9258) 2003 Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Martina JA., Moriyama K., Bonifacino JS. Cytosol from stably transfected cells was fractionated by size exclusion on superdex 200HR column, The fractions were analyzed vy western blot. fig 6a juan@helix.nih.gov J. Biol. Chem. (0021-9258) 2003 hps1_human Hermansky-Pudlak syndrome 1 protein HPS1 HPS protein protein human Homo sapiens Human MKCVLVATEGAEVLFYWTDQEFEESLRLKFGQSENEEEELPALEDQLSTLLAPVIISSMTMLEKLSDTYTCFSTENGNFLYVLHLFGECLFIAINGDHTESEGDLRRKLYVLKYLFEVHFGLVTVDGHLIRKELRPPDLAQRVQLWEHFQSLLWTYSRLREQEQCFAVEALERLIHPQLCELCIEALERHVIQAVNTSPERGGEEALHAFLLVHSKLLAFYSSHSASSLRPADLLALILLVQDLYPSESTAEDDIQPSPRRARSSQNIPVQQAWSPHSTGPTGGSSAETETDSFSLPEEYFTPAPSPGDQSSGSTIWLEGGTPPMDALQIAEDTLQTLVPHCPVPSGPRRIFLDANVKESYCPLVPHTMYCLPLWQGINLVLLTRSPSAPLALVLSQLMDGFSMLEKKLKEGPEPGASLRSQPLVGDLRQRMDKFVKNRGAQEIQSTWLEFKAKAFSKSEPGSSWELLQACGKLKRQLCAIYRLNFLTTAPSRGGPHLPQHLQDQVQRLMREKLTDWKDFLLVKSRRNITMVSYLEDFPGLVHFIYVDRTTGQMVAPSLNCSQKTSSELGKGPLAAFVKTKVWSLIQLARRYLQKGYTTLLFQEGDFYCSYFLWFENDMGYKLQMIEVPVLSDDSVPIGMLGGDYYRKLLRYYSKNRPTEAVRCYELLALHLSVIPTDLLVQQAGQLARRLWEASRIPLL 0885D3E2D64B3248 hps4_human Hermansky-Pudlak syndrome 4 protein Light-ear protein homolog HPS4 KIAA1667 protein protein human Homo sapiens Human MATSTSTEAKSASWWNYFFLYDGSKVKEEGDPTRAGICYFYPSQTLLDQQELLCGQIAGVVRCVSDISDSPPTLVRLRKLKFAIKVDGDYLWVLGCAVELPDVSCKRFLDQLVGFFNFYNGPVSLAYENCSQEELSTEWDTFIEQILKNTSDLHKIFNSLWNLDQTKVEPLLLLKAARILQTCQRSPHILAGCILYKGLIVSTQLPPSLTAKVLLHRTAPQEQRLPTGEDAPQEHGAALPPNVQIIPVFVTKEEAISLHEFPVEQMTRSLASPAGLQDGSAQHHPKGGSTSALKENATGHVESMAWTTPDPTSPDEACPDGRKENGCLSGHDLESIRPAGLHNSARGEVLGLSSSLGKELVFLQEELDLSEIHIPEAQEVEMASGHFAFLHVPVPDGRAPYCKASLSASSSLEPTPPEDTAISSLRPPSAPEMLTQHGAQEQLEDHPGHSSQAPIPRADPLPRRTRRPLLLPRLDPGQRGNKLPTGEQGLDEDVDGVCESHAAPGLECSSGSANCQGAGPSADGISSRLTPAESCMGLVRMNLYTHCVKGLVLSLLAEEPLLGDSAAIEEVYHSSLASLNGLEVHLKETLPRDEAASTSSTYNFTHYDRIQSLLMANLPQVATPQDRRFLQAVSLMHSEFAQLPALYEMTVRNASTAVYACCNPIQETYFQQLAPAARSSGFPNPQDGAFSLSGKAKQKLLKHGVNLL E47BCF49FC78830A hsp1-hsp4-1 9 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait physical association physical association Cells are stably transfected with HA-HPS1 hsp1-hsp4-2 9 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are stably transfected with myc-HPS4 hsp1-hsp4-3 1 11 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are stably transfected with HA-HPS1 and myc-HPS4 hsp1-hsp4-4 1 10 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are stably transfected with HA-HPS1 and myc-HPS4 hsp1-hsp4-5 5 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Proteins are endogenous hsp1-hsp4-6 5 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Proteins are endogenous hsp1-hsp4-7 2 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are transciently transfected with HA-HPS1 and myc-HPS4 The velocity fractions were analyzed first by immunoprecipitation against myc-HPS4 and second by western blotting analysis of HA-HPS1 The sedimentation coefficient is 6.3 S hsp1-hsp4-8 4 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are transciently transfected with HA-HPS1 and myc-HPS4 The velocity fractions were analyzed first by immunoprecipitation against myc-HPS4 and second by western blotting analysis of HA-HPS1 hsp1-hsp4-9 7 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are stably transfected with HA-HPS1 and myc-HPS4 The calculated molecular mass of the complex is ~175 kDa, which approximates the sum of the molecular maasses of the HSP1 and HSP4 polypeptides. the frictional ratio of ~1.8 indicates that is a moderately asymmetric complex. hsp1-hsp4-10 4 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are transciently transfected with HA-HPS1 and myc-HPS4 The velocity fractions were analyzed first by immunoprecipitation against HA-HPS1 and second by western blotting analysis of Myc-HPS4 hsp1-hsp4-11 6 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are stably transfected with HA-HPS1 and myc-HPS4 The size exclusion fractions were analyzed first by immunoprecipitation against myc-HPS4 and second by western blotting analysis of HA-HPS-1 hsp1-hsp4-12 6 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are stably transfected with HA-HPS1 and myc-HPS4 The size exlusion fractions were analyzed first by immunoprecipitation against myc-HPS1 and second by western blotting analysis of HA-HPS4 hsp1-hsp4-13 2 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are transciently transfected with HA-HPS1 and myc-HPS4 The velocity fractions were analyzed first by immunoprecipitation against HA-HPS1 and second by western blotting analysis of myc-HPS4 The sedimentation coefficient is 6.3 S hsp1-hsp4-14 3 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are transciently transfected with HA-HPS1 and myc-HPS4 hsp1-hsp4-15 8 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Cells are transciently transfected with HA-HPS1 and myc-HPS4