IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. EMBO Rep. (1469-221X) 2003 imex curation Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c esztul@uab.edu Only protein-protein interactions rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c estultz@uab.edu esztul@uab.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. EMBO Rep. (1469-221X) 2003 imex curation The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. EMBO Rep. (1469-221X) 2003 imex curation Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c esztul@uab.edu Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Garcia-Mata R., Sztul E. fig 1c estultz@uab.edu esztul@uab.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. EMBO Rep. (1469-221X) 2003 imex curation The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. EMBO Rep. (1469-221X) 2003 imex curation Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c esztul@uab.edu Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Garcia-Mata R., Sztul E. Rat liver cytosol was used for pulldown fig 1c estultz@uab.edu esztul@uab.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. EMBO Rep. (1469-221X) 2003 imex curation The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. EMBO Rep. (1469-221X) 2003 imex curation Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c esztul@uab.edu Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c estultz@uab.edu esztul@uab.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. EMBO Rep. (1469-221X) 2003 imex curation The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor. EMBO Rep. (1469-221X) 2003 imex curation Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c esztul@uab.edu Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Garcia-Mata R., Sztul E. Rat liver cytosol was used for co-immunoprecipitation fig 1c estultz@uab.edu esztul@uab.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. EMBO Rep. (1469-221X) 2003 imex curation uso1_human General vesicular transport factor p115 USO1 VDP Protein USO1 homolog Transcytosis-associated protein Vesicle-docking protein protein protein human Homo sapiens Human MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI C963652209031008 uso1_rat General vesicular transport factor p115 Uso1 Vdp Protein USO1 homolog Transcytosis-associated protein Vesicle-docking protein protein protein rat Rattus norvegicus (Rat) Rat MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIAYALDTLYNIISNDEEEEVEENSTRQSEDLGSQFTEIFIKQPENVTLLLSLLEEFDFHVRWPGVRLLTSLLKQLGPPVQQIILVSPMGVSKLMDLLADSREIIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIITEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKAWFEVGDENPGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGIPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNEKGQGEIVATLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQGNATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLENYTKEKLKQLIEKRIGKENYIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELKQQVSTLKCQNEQLQTAVTQQASQIQQHKDQYNLLKVQLGKDNHHQGSHSDGAQVNGIQPEEISRLREEIEELRSHQVLLQSQLAEKDTVIENLRSSQVSGMSEQALATCSPRDAEQVAELKQELSALKSQLCSQSLEITRLQTENSELQQRAETLAKSVPVEGESELVTAAKTTDVEGRLSALLQETKELKNEIKALSEERTAIQKQLDSSNSTIAILQTEKDKLYLEVTDSKKEQDDLLVLLADQDQKILSLKSKLKDLGHPVEEEDESGDQEDDDDELDDGDRDQDI 356394B48C7E003B goga2_mouse Golgin subfamily A member 2 Golga2 130 kDa cis-Golgi matrix protein protein protein mouse Mus musculus Mouse MWPPRFPPPRPGMSEETRQSKLAAAKKKLREYQQKNSPGVPAGAKKKKKIKNGHSPERPTASDCQSPENVPTDHIAPAPPTAATDTMFLGVTPSPDADLTQSHDAGNCSNLMEETKTFSSTESLRQLSQQLNGLVSESTSYINGEGLTSSNMKELENRYQELAVALDSSYVTNKQLSSTIEELKQQNQDTLDQLEKEKKDYQQKLAKEQGSLREQLQVHIQTIGILVSEKAELQTALAHTQQAARQKAGESEDLASRLQSSRQRVGELERTLSTVSTQQKQADRYNKDLTKERDALKLELYKNSKSNEDLRQQNSELEEKLRVLVAEKAAAQLGVEELQKKLEMSELLLQQFSSQSSAAGGNEQLQHAMEERAQLETHVSQLMESLKQLQVERDQYAENLKGESAMWQQRVQQMAEQVHTLKEEKEHRERQVQELETSLAALRSQMEEPPPPEPPAGPSEAEEQLQGEVEQLHKELERLTGQLRAQVQDNESLSHLNREQEGRLLELEREAQRWSEQAEERKQILESMQSDRTTISRALSQNRELKEQLAELQNGFVRLTNENMEITSALQSEQHVKKELARKLGELQERLGELKETVELKSQEAQGLQEQRDQCLSHLQQYAAAYQQHLAAYEQLTSEKEAIHKQLLLQTQLMDQLQHEEVQGKMAAELARQELQEAQERLKATSQENQQLQAQLSLLVLPGEGDVDQEEEDEEVPQSSLAIPEDLDSREAMVAFFNAAIARAEEEQARLRVQLKEQKARCRSLSHLAAPVQSKLEKEAVVPRNVDDSASEESNQALHVAMEKLQSRFLEVMQEKVELKERVEELEHCCIQLSGETDTIGEYIALYQNQRAVLKARHLEKEEYISRLAQDKEEMKVKLLELQELVLRLVNERNEWQGKFLAVSQNPGDVLTPVPTGSQEFGAADQQDDLREVSLADDIEPAQGEAGVPAPHENPTAQQIMQLLREIQNPRERPGLGSNPCIPFFYRADENDEVKIMVV 0D84161E727EA9F5 gbf1_human Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 GBF1 KIAA0248 protein protein human Homo sapiens Human MVDKNIYIIQGEINIVVGAIKRNARWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSHKYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN 5015D2BD70009CFA f1m8x9_rat Gbf1 protein protein rat Rattus norvegicus (Rat) Rat MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSHLKEVLNSGTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSCALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNLKKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVMPGSELPTPNGAALSSNLTGGMPFIEAPTSISSASSEAASTVVSPCTDSGLELSSQATSKEDLTDLEQAGSPREITTTEPGSTEIGVSDQLDPQEGAHVEKAQSASVESIPEVLEECTSPTDHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNTLNQQEKKETARPGFEAVDGSPDTYKSERAASDGKATGVPSDAPGLHFSSGGWLSTEHGKPGCSDLEEAGDSGVDKKTTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADMANSGWLVVGKDDIDNSKPAAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRGKSHKYDSKGNRFKKKPKEGSMLRRPRASSQHATRAGHSDEEEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLETGGRKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAVPESLKNMLLVMDTAEIFHSADARGGSPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPTEPHSQKPLASTHLTPAAGDPRMPGHPPPPEIPSEVGACDPEKPESTRAASSSSPGSPVASSPSKQSPAPEGPPPLAQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN 6D8182D34596497B q6dfz1_mouse Gbf1 protein protein mouse Mus musculus Mouse MVDKNIYIIQGEINIVVGAIKRNARWSTHIPLDEERDPLLHSFSNLKEVLNSVTELSEIEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHTTKVTPGSELPTPNGATLPSNLTGGVPFIDAPTSISSASSEAASTVVSPCTDSGLELSSQTTSKEDLTDLEQAGSPREITTSEPGSSEMGASDQLDPQEGAHVEKAQSASVESIPEVLEECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLNLYAASLRVCFLLFESMREHLKFQLEMYIKKLMEIITVENPKMPYEMKEMALEAVVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTESHCQAKVLNTLNQQEKKETARPGFEAVDGNPETNKSERATSDGKSTGVALDARGLHFPSGGWLSTEHGKPGCRDLEEAGDSGADKKFTRKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNMDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEVFTEHWRSCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVWSVLLHRGASPEGVFLRVPPGSYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSVRGPSTENQEAKRVALDCIKQCDPEKMITESKFLQLESLQELMKALVSVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPDALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDSEVYTDHGRPGKIHRSATDADMVNSGWLVVGKDDIDNSKPGAGLSRPGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQDKRSKSHKYDSKGNRFKKKPKEGSMLRRPRGSNQHATRGGHSDEEEDEGVPASYHTVSLQVSQDLLDLMHTLHTRAASIYSSWAEEQRHLEGGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPAEPHSQKPLASTHLTSAAGDPRMPGHPPLPEIPSEMGVCDSEKPESTRAPSSSSPGSPMASSPSKLSPAQEGPPPLTQPPLILQPLTSPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN 5E6FFE749D61938E uso1-gbf1-1 2 7 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 20 out of the 40 positive clones in the Y2H screening were identified as GBF1 ORFs. uso1-golga2-2 2 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 3 out of the 40 positive clones in the Y2H screening were identified as GM130 ORFs. uso1-gbf1-3 3 11 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey rat-liver rattus norvegicus liver Rat liver liver physical association physical association Fig. 1B uso1-gbf1-4 1 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig. 1C argef1-gbf1-5 5 7 unspecified role unspecified role bait bait n-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2 argef1-gbf1-6 4 6 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 3B GBF1 and p115 colocalizes extensively in the golgi and in periphericalvesicular tubular clusters (VTCs).