IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cloning and expression pattern of a novel PEBP2 beta-binding protein (charged amino acid rich leucine zipper-1[Crl-1]) in the mouse. Cloning and expression pattern of a novel PEBP2 beta-binding protein (charged amino acid rich leucine zipper-1[Crl-1]) in the mouse. Mech. Dev. (0925-4773) 2001 Sakuma T., Li QL., Jin Y., Choi LB., Kim EG., Ito K., Ito Y., Nomura S., Bae SC. A mouse brain cDNA library was used in the detection of Crlz1. Two proteins involved: Crlz1 and Cbfb. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Sakuma T., Li QL., Jin Y., Choi LB., Kim EG., Ito K., Ito Y., Nomura S., Bae SC. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). A mouse brain cDNA library was used in the detection of Crlz1. Two proteins involved: Crlz1 and Cbfb. Mech. Dev. (0925-4773) 2001 sas10_mouse Something about silencing protein 10 Crlz1 Disrupter of silencing SAS10 UTP3 homolog Charged amino acid-rich leucine zipper 1 Utp3 Sas10 Crl1 protein protein mouse Mus musculus Mouse MVKKSRRRGAAQWAAVRAQAGLTATDENEDDLGLPPSPGDSSYYQDQVDEFHEARSRAVLAKGWNEVESGEEDGDEEEEVLPLDIDDGDDEDGESSEEEEVGEDDDGGSSVQSEAEASVDPSLSWGQRKKLYYDTDYGSKSRGRQSQQEVEEEEREEEEEAQIIQRRLAQALQEDDFGVAWVEAFAKPVPQVDEAETRVVKDLAKVSVKEKLKMLKKESPELLELIEDLQAKLTEVKDELEPLLQLVEKGVIPTGRGSEYLKTKYNLYLNYCANISFYLILKARRVPAHGHPVIERLVTYRNLINKLSVVDQKLSSEIRHLLTAKDGAVKKEMTPKAKLTKTKPKSVKQAAAVALTDEPDFDGAALKYYKEMEDRQELKRKKEENSAEEQALEEQNAKRAITYQIAKNRGLTPRRKKIDRNPRVKHREKFRKAKIRRRGQVREVRREEQRYSGELSGIRAGVKKSIKLK D469094FF0AB9632 pebb_mouse Core-binding factor subunit beta Polyomavirus enhancer-binding protein 2 beta subunit SL3-3 enhancer factor 1 subunit beta SL3/AKV core-binding factor beta subunit Cbfb Pebp2b Pebpb2 protein protein mouse Mus musculus Mouse MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGGGDDLKLR 0B2E6101A35D0FD8 crlz1-cbfb-1 1 2 unspecified role unspecified role prey prey in vitro In vitro 3 unspecified role unspecified role bait bait crlz1 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association The Y2H beta-galactosidase activity assay was used to determine the region of interaction between the three alternate splice variants of cbfb and crlz1. This showed that crlz1 interacts with a 17aa region unique only to the c-terminus of the second alternate splice variant of cbfb.