<?xml version='1.0' encoding='UTF-8'?>
<entrySet xmlns="http://psi.hupo.org/mi/mif300" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/mi/mif300 https://raw.githubusercontent.com/HUPO-PSI/miXML/master/3.0/src/MIF300.xsd" level="3" version="0" minorVersion="0">
  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
      </bibref>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0469" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-10" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="22121220" refType="method reference" refTypeAc="MI:0357"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="19850723" refType="method reference" refTypeAc="MI:0357"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Neurofilament protein heterotetramers as assembly intermediates.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="7721855" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1992850" refType="identity" refTypeAc="MI:0356"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Neurofilament protein heterotetramers as assembly intermediates.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
            <attribute name="publication year" nameAc="MI:0886">1995</attribute>
            <attribute name="author-list" nameAc="MI:0636">Cohlberg JA., Hajarian H., Tran T., Alipourjeddi P., Noveen A.</attribute>
            <attribute name="exp-modification" nameAc="MI:0627">The components of the interaction (complex) were separated by electrophoretic native gel in 2M urea</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="7721855" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>affinity chrom</shortLabel>
            <fullName>affinity chromatography technology</fullName>
            <alias type="go synonym" typeAc="MI:0303">Affinity purification</alias>
            <alias type="synonym" typeAc="MI:1041">Affinity purification</alias>
            <alias type="synonym" typeAc="MI:1041">affinity chrom</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0004" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-86" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="7708014" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Cohlberg JA., Hajarian H., Tran T., Alipourjeddi P., Noveen A.</attribute>
          <attribute name="exp-modification" nameAc="MI:0627">The components of the interaction (complex) were separated by electrophoretic native gel in 2M urea</attribute>
          <attribute name="accepted"/>
          <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
          <attribute name="publication year" nameAc="MI:0886">1995</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>nfm_bovin</shortLabel>
          <fullName>Neurofilament medium polypeptide</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Neurofilament triplet M protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">160 kDa neurofilament protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Neurofilament 3</alias>
          <alias type="gene name" typeAc="MI:0301">NEFM</alias>
          <alias type="gene name synonym" typeAc="MI:0302">NEF3</alias>
          <alias type="gene name synonym" typeAc="MI:0302">NFM</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="O77788" version="SP_54" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-444343" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005198" version="SP_130"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005883" version="SP_130"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030424" version="SP_130"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0033693" version="SP_130"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR006821"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR018039"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR002957"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR039008"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027697"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9913">
          <names>
            <shortLabel>bovin</shortLabel>
            <fullName>Bos taurus (Bovine)</fullName>
            <alias type="synonym" typeAc="MI:1041">Bovine</alias>
          </names>
        </organism>
        <sequence>MSYTLDSLGNPSAYRRVTETRSSFSRISGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLTYSSAMLSSAESSLDFSQSSSLLDGGSGPGGDYKLSRSNEKEQIQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPEAKSPTAKSPTAKSPVAKSPTAKSPEAKSPEAKSPTAKSPTAKSPAAKSPAPKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKPKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD</sequence>
        <attributeList>
          <attribute name="crc64">B1C2FA96E31793C0</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>nfl_bovin</shortLabel>
          <fullName>Neurofilament light polypeptide</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Neurofilament triplet L protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">68 kDa neurofilament protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Micro glutamic acid-rich protein</alias>
          <alias type="gene name" typeAc="MI:0301">NEFL</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P02548" version="SP_68" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAP00000029264" version="SP_171" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P79127" version="SP_68" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q17QQ0" version="SP_68" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-444335" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAG00000021949" version="SP_171" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAT00000029264" version="SP_171" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000226" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005200" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005882" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005883" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008022" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008089" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008090" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0014012" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019896" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030424" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030674" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0031133" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0031594" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0033693" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0040011" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043524" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0045109" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0045110" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0048812" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0050772" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0050885" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0060052" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0060074" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0098981" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0099160" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0099182" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0099184" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1904115" version="SP_171"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR006821" version="SP_171"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR018039" version="SP_171"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027692" version="SP_171"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR039008" version="SP_171"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0098685" version="SP_171"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_776546.1"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9913">
          <names>
            <shortLabel>bovin</shortLabel>
            <fullName>Bos taurus (Bovine)</fullName>
            <alias type="synonym" typeAc="MI:1041">Bovine</alias>
          </names>
        </organism>
        <sequence>MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLESLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSLTTGYTQSSQVFGRSAYGGLQTSSYLMSARSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKEKEEAEAEAEAEAEAEAEEEEGAQEEEAAKEDAEEAKEEEGGEGEEAEETKEAEEEEKKDEGAGEEQATKKKD</sequence>
        <attributeList>
          <attribute name="crc64">D772A27EE1F1DCD3</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>nfl-nfm-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-444354" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="6">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">NFL also form a complex with NFH</attribute>
          <attribute name="remark-internal">add interaction when bovin NFH available</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>