IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. Dev. Cell (1534-5807) 2004 Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Dev. Cell (1534-5807) 2004 Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. Dev. Cell (1534-5807) 2004 Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp drome-ovary Drosophila melanogaster ovary ovary Ovary coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Dev. Cell (1534-5807) 2004 Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis. Dev. Cell (1534-5807) 2004 Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp in vitro In vitro pull down pull down predetermined predetermined participant predetermined Nakamura A., Sato K., Hanyu-Nakamura K. akiran@cdb.riken.jp Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Dev. Cell (1534-5807) 2004 cup_drome Protein cup cup fs(2)cup Oskar ribonucleoprotein complex 147 kDa subunit CG11181 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQMAEAEQENGAGALKIATNAGATDRPAHQQLPLPVEDQQDEVLTPAEKGKFEYPPPPPPPTPVQAPLATKATALNASQEHDDDEANSEKWEDPCAPPPPPPLPTSAFLATGLGYLKLPAFKLKDALEKAITKLEANKRTLKASPESSRSIKNKNVVALEMLPRRSNPETIGDGSMLASTSTAVMLQTKKPAVIVEMERRCKIINLLAKQNQILESISGEAIPMHGPSKHLHEDEGLTLQVLSARASTPYTQPSSMLSCTAVSCDLEHDSPRKQVASKEAVPEQQSSQVQQKRPPSTGIHKPGSLRAPKAVRPTTAPVVSSKPVKSYTRSRLMDIRNGMFNALMHRSKESFVMPRIATCDDIELEGRLRRMNIWRTSDGTRFRTRSTTANLNMNNNNNNECMPAFFKNKNKPNLISDESIIQSQPPQPQTEFQDPAIVNQRRIGSGRLNHSKWGYNDEDYHSYHNGKSQHMEEVNSKNSKNMTVLQFFDNGEISSQPQRRPNTPVMGMSINRSENDTLHSNESSEDLSRANENYVKRVMSGFLVVSKPKSRDVEDRHHRRYRNQNEEPEWFSCGPTSRLDTIELCGFDEDEEKMLKEGNKNHGLGETERETSKQKMDHKYKWTHAEPMGRSKYMPKHDTNNNHNVENMNNVMATEHQQQKEEKRPGSGRSFQFDKFNQSQQNYESSSYVNHQQPPQTQPQQMQQQSNTNTNNSKFMSFFANEGNSSSSSLNEFFKQAINQGHGNNPEQPKSLGHIGQMPSVDQLEAKWRRNSLNNVGETANKQTDNFQKLIGSLSSAKPQSQAVGYDAISNFIMQQQQYQQQQQKQHLIIQQQQQHTAFLASLQLKAILGRADTQLLLLRLTKGEISKHGLLVQLANPRLTDMDREAITAVLQFTNTQQQQQQHKQQLDMLSSTVIASQLQNLHNLAIVQQTLAARQQPQHNPQTQAPHQLSQEDLQAHANVIMRNAVMKRKIEEQTSKLINGGAKHQAQQQYLNRGQQRQARPDANSNALLHALISGGGNNHASGYPMNGQPQKHHSNLRFGDNQNFQSFESNQPHFATQYKQQYQQSQQQHPHQQPQQLNSLHQNNAGAVNSFNKAQMQAQSAISMLPNSGDEFH 91274997F199EFD3 if4e_drome Eukaryotic translation initiation factor 4E1 eIF4E1 Eif4e eIF-4E eIF-4F 25 kDa subunit mRNA cap-binding protein CG4035 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSDFHRMKNFANPKSMFKTSAPSTEQGRPEPPTSAAAPAEAKDVKPKEDPQETGEPAGNTATTTAPAGDDAVRTEHLYKHPLMNVWTLWYLENDRSKSWEDMQNEITSFDTVEDFWSLYNHIKPPSEIKLGSDYSLFKKNIRPMWEDAANKQGGRWVITLNKSSKTDLDNLWLDVLLCLIGEAFDHSDQICGAVINIRGKSNKISIWTADGNNEEAALEIGHKLRDALRLGRNNSLQYQLHKDTMVKQGSNVKSIYTL DIP protein P48598 original sequence version: 96 B844B2DD5738758E o18409_drome bru1 aret bruno CG31762 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLSSLDALAGKIATATPGTGAVTADSKNTQSLHHHHRLDYDYSAVEIEPPAEQLANSPRSERERLQQAQQAYEQQAQAELEFGLAEVDAKLTQLKSSHSLQHHHSHHHQLQRHTALHQQQQQLHHHQPHHNHHHHHPYQRPRPTSPPQQQTSTYQQQLSQLAALQDHHDLLQTQQELFHKQLATMQEYQRERERERERERERERERERDRSSFIDNSDYDSQTEFKICLRDSAFGMSDKSSSATNSLPNSPIHSNNNNPSLLNNNNNNSNGTSSNNSLNVGNNSSNPSLGGTNSNALVSVGSNGIMSAAGLVNNNNNPCSANRNVVAMVDDDACFRLDTDATVTYGEKEPDPDNIKMFVGQVPKSMDESQLREMFEEYGAVHSINVLRDKATGISKGCCFVTFYTRRAALKAQDALHNVKTLNGMYHPIQMKPADSENRNERKLFVGMLNKKLNENDVRKLFEVHGAIEECTVLRDQNGQSKGCAFVTFATKHAAISAIKVTLSQNKIMEGCTSPLVVKFADTQKEKEQKKIQQIQANLWNLASNINIPLGQTATSVTTPILPNPAQQPSPVLGADAITPASIQLLQQLQAVGLQHQLLQALTGLGAQQSSSATDTSAVAAAGLLTPMTVQNLAAIAAMTQPSLSNAAAAAARATSPGSAQLTNTAALLWSDPNPMASAYMSTAAGLPQFGSASALSTSPLASVALSAAAAAAAGKQIEGPEGCNLFIYHLPQEFTDTDLASTFLPFGNVISAKVFIDKQTSLSKCFGFVSFDNPDSAQVAIKAMNGFQVGTKRLKVQLKKPKDSKPY 55DAD7C50F9BADC6 ddx6_drome ATP-dependent RNA helicase me31b me31B CG4916 Maternal expression at 31B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMTEKLNSGHTNLTSKGIINDLQIAGNTSDDMGWKSKLKLPPKDNRFKTTDVTDTRGNEFEEFCLKRELLMGIFEKGWERPSPIQEAAIPIALSGKDVLARAKNGTGKTGAYCIPVLEQIDPTKDYIQALVMVPTRELALQTSQICIELAKHLDIRVMVTTGGTILKDDILRIYQKVQLIIATPGRILDLMDKKVADMSHCRILVLDEADKLLSLDFQGMLDHVILKLPKDPQILLFSATFPLTVKNFMEKHLREPYEINLMEELTLKGVTQYYAFVQERQKVHCLNTLFSKLQINQSIIFCNSTQRVELLAKKITELGYCCYYIHAKMAQAHRNRVFHDFRQGLCRNLVCSDLFTRGIDVQAVNVVINFDFPRMAETYLHRIGRSGRFGHLGIAINLITYEDRFDLHRIEKELGTEIKPIPKVIDPALYVANVGASVGDTCNNSDLNNSANEEGNVSK B783E8185EF11E86 cup-aret-1 1 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association AA 821-1132 of Cup used as bait. AA 320-520 of Bru used as prey. Revisit when Protein Editor in place cup-eif4e-1 3 4 unspecified role unspecified role prey prey in vitro In vitro 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Full length cup cDNA was used to synthesize Cup protein in vitro using TNT T7 Quick kit. GST-EIF4E were expressed in E. coli. The interaction was RNase resistant. cup-eif4e-2 2 7 unspecified role unspecified role bait bait in vitro In vitro 5 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary 4 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary association association Similar interaction was observed using anti eIF4E. The interaction was RNase sensitive. cup-eif4e-3 2 6 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary 7 unspecified role unspecified role bait bait in vitro In vitro 4 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary 5 unspecified role unspecified role prey prey drome-ovary Drosophila melanogaster ovary ovary Ovary association association This complex was RNase sensitive. However, the complex between eIF4E-Cup-Bru is RNase resistant