IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. Genome Res. (1088-9051) 2003 Zhong J., Zhang H., Stanyon CA., Tromp G., Finley RL. A modification of the pooling method and array method was used in which a small pool of AD constructs (96) is mated with an array of BD constructs and then the BD which shows interaction is mated with an array of the AD constructs to identify the interacting partners. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zhong J., Zhang H., Stanyon CA., Tromp G., Finley RL. A modification of the pooling method and array method was used in which a small pool of AD constructs (96) is mated with an array of BD constructs and then the BD which shows interaction is mated with an array of the AD constructs to identify the interacting partners. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Genome Res. (1088-9051) 2003 q9gtq8_drome CycD CG9096 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MADIMDLLCSEIIVYDSDPSLYRLNKRQQQMSSPAIIRDQSSICSSLGDAESDSHPDAQNNAVVCNMKELVYIYASVDSAAKNPEQLEPPPPPPPPPPPPPTATQSIQSYPRYISQEPPTSHCQRLDERLTTTADPPATDNVNTAIGDPTLYSDRCLENFLKVEEKHHKIPDTYFSIQKDITPPMRKIVAEWMMEVCAEENCQEEVVLLALNYMDRFLSSKSVRKTQLQILAAACLLLASKLREPSCRALSVDLLVVYTDNSIYKDDLIKWELYVLSRLGWDLSSVTPLDFLELLMMRLPIGSKNFPDINIGKVRGHAQAFISLAAKEHKFAKFSASTIAASSIAASMNGLKWHLRSGHNLHFLLSLMTDLTSVEQAQVRDCMLHMEDIFKEHSRNLEPFLVNIDPKEMSTLYYKRRFQIHQSQHLSQISIRPLPLPKAPAECVEQHQQQHNFGSAAPHRTHTCKMQAQAQAQNEIQDVTF CD91A6F1FFE94133 q8ms31_drome mute CG15610 CG33455 CG33456 Dmel\CG34415 Mute CG34415 Dmel_CG34415 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFCSNTNSKTAAQPKRSRSDDEAAKLRRANRQEEMLRQMLQPDTTEERNRKDAIFAMNFYEKVEEALLSANRLDDCKKFNSILQNFDPRQEKVSSLYLKVEEILLPTHPELAEVFLNFLLPAEAAEMGKFFEHFMICNATNFINKLNIYFSKQPAQIRKIYACLNELAEMPNVSMKKVENKIMPLLKGNQFLIDWFVQQFPQGKPPKRILSPVETINLHDVQNNTTGEYTETIQEFTDVPTPSKPGCHLKYINGRIFYGAKILLPAKLSFMAASIYNEQSHDVPPVSSDSINCAHGIRAQGEKLLSMASNTDSDDGADRSEEDDASRALIIDTTSDEQNSCSSIEMCDETGLRAHAIRLNPGLYTNSSFNNAATSTPNLGNSHQSHHPHAKKTAINFGEISPRKQSAFNNSGLSPLSAAPNSSPQVDKRKSPTKKIRSPLNQTQSRQRANNQPMVVIHESQPSSSPAIECAKRLRTLIDQESVRDAADTKAGIRIIAQAKQQPPNQCSSEAITKVEVVSLDESAENMPFCTNLKMDTDQSEEELEPLQLSAGNTPQHVITTQFDSDEDCKLDVVATLANCTTTEDRSESAAALPATTSHSESGSSHSAPWTREEDKVILIEMKIGARDREQLIRRMRAKLKNRSIEELRSRHQFLMDFLSKLQGK 1DD178D4705918CB q24159_drome CycJ CG10308 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly FNFDTMEQKVAAEQNIFVVDRKLKKTCPQADVERLAKTHWLTDYARDIFLTMREQELSRRPLFYLSPQLNERRRMLQLLKLATSAHKLSRLALHLAVYYMDRFVDYYKIRPDKLLLVAITCLHIAAQIENTDAFIPRYSEMNRLVKNAYTAFEYKAVERKILCFLNFELIRPTTASFVELFACSFLTRSDFKNYIEMLDEYERIHHTQPYQRYISFEEMLSILAQLLLRMADYTLYISRFANDLPSLLAAACIAAVRQVSGVRRWSEYLVGLTSYTEAHVEPYMNVLTDYHYYHVIQADYGSPSVQTNQSLASPDSGFEESFTENTNLVVSDEVVTVETYNIITVQLQDPSPHSSTFLPKEQTNLKRSRFEDDTENQHPLKHAKVESVAKD DFCD3FEF0EE56F44 cdk2_drome Cyclin-dependent kinase 2 Cdk2 Cell division control protein 2 cognate cdc2c CG10498 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPSTAIREISLLKNLKHPNVVQLFDVVISGNNLYMIFEYLNMDLKKLMDKKKDVFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFGLARAFNVPMRAYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMIMRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFKTKFPRWEGTNMPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPVDPNAGSASRLTRLV DIP protein P23573 original sequence version: 103 576A88767F9D35C0 cg15610-cycd-1 1 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Interaction scored as growth on LEU and X-Gal activity. cdc2c-cyc5-1 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Interaction scored as growth on LEU and X-Gal activity.