IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Brower CS., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R., Klausner RD., Malik S., Lane WS., Sorokina I., Roeder RG., Conaway JW., Conaway RC. The yeast strain used for this study constitutively expresses Elongin C. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 elob_human Elongin-B ELOB Elongin 18 kDa subunit RNA polymerase II transcription factor SIII subunit B SIII p18 TCEB2 Transcription elongation factor B polypeptide 2 protein protein human Homo sapiens Human MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVQ C045F58FBED0EC47 med10_human Mediator of RNA polymerase II transcription subunit 10 MED10 L6 TRG17 Mediator complex subunit 10 Transformation-related gene 17 protein Transformation-related gene 20 protein TRG20 protein protein human Homo sapiens Human MAEKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIRGEDHPPS DIP protein Q9BTT4 original sequence version: 95 F804CD76770149E1 rbx1_human E3 ubiquitin-protein ligase RBX1 RBX1 RNF75 ROC1 Regulator of cullins 1 RING finger protein 75 Protein ZYP RING-box protein 1 E3 ubiquitin-protein transferase RBX1 protein protein human Homo sapiens Human MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH DIP protein P62877 original sequence version: 120 DIP protein P62877 original sequence version: 115 DIP protein P62877 original sequence version: 78 DIP protein P62877 original sequence version: 113 DIP protein P62877 original sequence version: 75 DIP protein P62877 original sequence version: 86 DIP protein P62877 original sequence version: 136 DIP protein P62877 original sequence version: 126 DIP protein P62877 original sequence version: 90 DIP protein P62877 original sequence version: 114 30FC5ADF66096C0E med17_human Mediator of RNA polymerase II transcription subunit 17 TRAP80 MED17 ARC77 DRIP80 Mediator complex subunit 17 Cofactor required for Sp1 transcriptional activation subunit 6 Transcriptional coactivator CRSP77 Vitamin D3 receptor-interacting protein complex 80 kDa component Thyroid hormone receptor-associated protein complex 80 kDa component Activator-recruited cofactor 77 kDa component CRSP6 DRIP77 protein protein human Homo sapiens Human MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQEWPGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQNPQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL DIP protein Q9NVC6 original sequence version: 120 DIP protein Q9NVC6 original sequence version: 114 DIP protein Q9NVC6-1 original sequence version: 77 DIP protein Q9NVC6 original sequence version: 74 47011210952D287B cul2_mouse Cullin-2 Cul2 protein protein mouse Mus musculus Mouse MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHVRHLYKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQNILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHSECHSIIQQERKNDMANMYVLLRAVSSGLPHMIEELQKHIHDEGLRATSNLTQEHMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDKLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIRNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMSFSSKRTKFKITTSMQKDTPQELEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA 311C05CC262692E0 eloc_human Elongin-C ELOC Elongin 15 kDa subunit RNA polymerase II transcription factor SIII subunit C SIII p15 TCEB1 Transcription elongation factor B polypeptide 1 protein protein human Homo sapiens Human MDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC 98D88696E883538B med8_human Mediator of RNA polymerase II transcription subunit 8 MED8 Mediator complex subunit 8 Activator-recruited cofactor 32 kDa component protein protein human Homo sapiens Human MQREEKQLEASLDALLSQVADLKNSLGSFICKLENEYGRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGLSNWRPSGSSGPGQAGQPGAGTILAGTSGLQQVQMAGAPSQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR 6C186A4CECD1AFB3 tceb2-med8-1 1 5 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association EloC: Q15369 essential for this interaction. med8-nut2-1 3 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association tceb1-med8-1 1 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association EloB: Q15370 essential for this interaction. tceb2-med8-2 1 11 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association EloC: Q15369 essential for this interaction. tceb1-tceb2-1 2 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association Epitope tagged T7-MED8, myc-EloB, HSV-EloC proteins used in the study Revisit when protien Editor in place. The Elongin BC-box motif SLNKMCSNLL located between AA 142-151 important for this interaction. tceb1-tceb2-2 2 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association Epitope tagged T7-MED8, myc-EloB, HSV-EloC proteins used in the study Revisit when protien Editor in place. The Elongin BC-box motif SLNKMCSNLL located between AA 142-151 important for this interaction. tceb1-tceb2-3 4 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Revisit when protien Editor in place. The Elongin BC-box motif SLNKMCSNLL located between AA 142-151 important for this interaction. Epitope tagged T7-MED8, HA-Cul2, HPC4-EloB, HSV-EloC, Myc-Rbx1 proteins used in the study tceb1-tceb2-4 4 11 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Revisit when protien Editor in place. The Elongin BC-box motif SLNKMCSNLL located between AA 142-151 important for this interaction. Epitope tagged T7-MED8, HA-Cul2, HPC4-EloB, HSV-EloC, Myc-Rbx1 proteins used in the study tceb1-tceb2-5 4 11 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association Revisit when protien Editor in place. The Elongin BC-box motif SLNKMCSNLL located between AA 142-151 important for this interaction. Epitope tagged T7-MED8, HA-Cul2, HPC4-EloB, HSV-EloC, Myc-Rbx1 proteins used in the study