MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Genes Dev. (0890-9369) 2002 imex curation Holm M., Ma LG., Qu LJ., Deng XW. xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Holm M., Ma LG., Qu LJ., Deng XW. A directed yeast two hybrid assay was used in this study. Figure 1 (c). xingwang.deng@yale.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Genes Dev. (0890-9369) 2002 imex curation Holm M., Ma LG., Qu LJ., Deng XW. xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Holm M., Ma LG., Qu LJ., Deng XW. Figure 8 (b). xingwang.deng@yale.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Genes Dev. (0890-9369) 2002 imex curation Holm M., Ma LG., Qu LJ., Deng XW. xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Holm M., Ma LG., Qu LJ., Deng XW. xingwang.deng@yale.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis. Genes Dev. (0890-9369) 2002 imex curation Holm M., Ma LG., Qu LJ., Deng XW. xingwang.deng@yale.edu An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Only protein-protein interactions imex curation allce Allium cepa (Onion) Onion fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Holm M., Ma LG., Qu LJ., Deng XW. The FRET analysis was carried out in onion epidermis cells. Constructs were introduced into the cells by partical bombardment. Figure 2 (f). xingwang.deng@yale.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). An arabidopsis lambda ACT expression cDNA library was obtained from the Arabidopsis Biological Resource Center and converted to a pACT library prior to screening. Genes Dev. (0890-9369) 2002 IMEx hy5_arath Transcription factor HY5 HY5 BZIP56 At5g11260 F2I11_150 Protein LONG HYPOCOTYL 5 bZIP transcription factor 56 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL 1E3C4672B192D8A9 q8w191-1 Transcription factor HY5-like HYH BZIP64 HY5 homolog bZIP transcription factor 64 MKP6.27 At3g17609 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLQRPNGNSSSSSSHKKHKTEESDEELLMVPDMEAAGSTCVLSSSADDGVNNPELDQTQNGVSTAKRRRGRNPVDKEYRSLKRLLRNRVSAQQARERKKVYVSDLESRANELQNNNDQLEEKISTLTNENTMLRKMLINTRPKTDDNH FCAA23AFBED351B6 cop1_arath E3 ubiquitin-protein ligase COP1 COP1 Constitutive photomorphogenesis protein 1 RING-type E3 ubiquitin transferase COP1 T21L14.11 At2g32950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEISTDPVVPAVKPDPRTSSVGEGANRHENDDGGSGGSEIGAPDLDKDLLCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSDCPCCSQHLTNNQLYPNFLLDKLLKKTSARHVSKTASPLDQFREALQRGCDVSIKEVDNLLTLLAERKRKMEQEEAERNMQILLDFLHCLRKQKVDELNEVQTDLQYIKEDINAVERHRIDLYRARDRYSVKLRMLGDDPSTRNAWPHEKNQIGFNSNSLSIRGGNFVGNYQNKKVEGKAQGSSHGLPKKDALSGSDSQSLNQSTVSMARKKRIHAQFNDLQECYLQKRRQLADQPNSKQENDKSVVRREGYSNGLADFQSVLTTFTRYSRLRVIAEIRHGDIFHSANIVSSIEFDRDDELFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLSCLSWNKHEKNHIASSDYEGIVTVWDVTTRQSLMEYEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQEASVINIDMKANICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLHVFSGHKKAVSYVKFLSNNELASASTDSTLRLWDVKDNLPVRTFRGHTNEKNFVGLTVNSEYLACGSETNEVYVYHKEITRPVTSHRFGSPDMDDAEEEAGSYFISAVCWKSDSPTMLTANSQGTIKVLVLAA DIP protein P43254 original sequence version: 138 DIP protein P43254 original sequence version: 113 DIP protein P43254 original sequence version: 114 E6D9A1B702EAE8F0 hyh_arath Transcription factor HY5-like HY5 homolog HYH BZIP64 bZIP transcription factor 64 MKP6.27 At3g17609 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLQRPNGNSSSSSSHKKHKTEESDEELLMVPDMEAAGSTCVLSSSADDGVNNPELDQTQNGVSTAKRRRGRNPVDKEYRSLKRLLRNRVSAQQARERKKVYVSDLESRANELQNNNDQLEEKISTLTNENTMLRKMLINTRPKTDDNH FCAA23AFBED351B6 cop1-hyh-3 3 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association cop1-hyh-1 1 7 unspecified role unspecified role bait bait P43254:p.Lys422Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:36:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys422glu' Tue Jun 30 17:40:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys422Glu' region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P43254:p.Arg465Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Wed May 02 17:42:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg465glu' Tue Jun 30 18:38:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg465Glu' P43254:p.Trp467Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 17:43:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp467ala' Tue Jun 30 16:39:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp467Ala' P43254:p.Lys550Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E Wed May 02 19:05:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys550glu' Tue Jun 30 18:43:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys550Glu' P43254:p.Gly524Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G E Wed May 02 16:50:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly524glu' Tue Jun 30 16:29:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly524Glu' region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey Q8W191-1:p.[Val31Ala;Pro32Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain V A Fri Jul 22 10:51:23 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro32ala,val31ala' Wed May 02 20:23:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val31ala,pro32ala' Tue Jun 30 18:35:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val31Ala;Pro32Ala]' physical association physical association cop1-hyh-2 4 7 unspecified role unspecified role neutral component neutral component gfp-tag blue version of GFP gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component gfp-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Fig. 2F hy5-hyh-3 2 5 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association