IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Cell (0092-8674) 2000 Bowman AB., Kamal A., Ritchings BW., Philp AV., McGrail M., Gindhart JG., Goldstein LS. mouse-brain Mus musculus brain brain Brain [cerebrum] coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Bowman AB., Kamal A., Ritchings BW., Philp AV., McGrail M., Gindhart JG., Goldstein LS. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2000 Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Cell (0092-8674) 2000 Bowman AB., Kamal A., Ritchings BW., Philp AV., McGrail M., Gindhart JG., Goldstein LS. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bowman AB., Kamal A., Ritchings BW., Philp AV., McGrail M., Gindhart JG., Goldstein LS. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2000 Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Cell (0092-8674) 2000 Bowman AB., Kamal A., Ritchings BW., Philp AV., McGrail M., Gindhart JG., Goldstein LS. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Bowman AB., Kamal A., Ritchings BW., Philp AV., McGrail M., Gindhart JG., Goldstein LS. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2000 klc1_mouse Kinesin light chain 1 Klc1 Kns2 protein protein mouse Mus musculus Mouse MYDNMSTMVYIKEEKLENVTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSSMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQNVRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDSDSSKEPLDDLFPNDEDEPGQGIQHSDSSAAAARQGYEIPARLRTLHNLVIQYASQGRYEVAVPSCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALGIYQTKLGPDRTPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEAEFGSVDDENKPIWMHAEEREECKGKQKDGSAFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPESMEKRRSRESLNMDVVKYESGPDGGEEA DIP protein O88447 original sequence version: 83 550F23AB36049C03 jip3_mouse C-Jun-amino-terminal kinase-interacting protein 3 Syd2 Mapk8ip3 Jip3 Jsap1 JNK MAP kinase scaffold protein 3 Mitogen-activated protein kinase 8-interacting protein 3 JNK/SAPK-associated protein 1 Sunday driver 2 protein protein mouse Mus musculus Mouse MMEIQMDEGGGVVVYQDDYCSGSVMSERVSGLAGSIYREFERLIHCYDEEVVKELMPLVVNVLENLDSVLSENQEHEVELELLREDNEQLLTQYEREKALRKQAEEKFIEFEDALEQEKKELQIQVEHYEFQTRQLELKAKNYADQISRLEERESEMKKEYNALHQRHTEMIQTYVEHIERSKMQQVGGSGQTESSLPGRSRKERPTSLNVFPLADGMVRAQMGGKLVPAGDHWHLSDLGQLQSSSSYQCPNDEMSESGQSSAAATPSTTGTKSNTPTSSVPSAAVTPLNESLQPLGDYVSVTKNNKQAREKRNSRNMEVQVTQEMRNVSIGMGSSDEWSDVQDIIDSTPELDVCPETRLERTGSSPTQGIVNKAFGINTDSLYHELSTAGSEVIGDVDEGADLLGEFSVRDDFFGMGKEVGNLLLENSQLLETKNALNVVKNDLIAKVDQLSGEQEVLKGELEAAKQAKVKLENRIKELEEELKRVKSEAVTARREPREEVEDVSSYLCTELDKIPMAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASREHPSVQEKKKSTIWQFFSRLFSSSSSPPPAKRSYPSVNIHYKSPTAAGFSQRRSHALCQISAGSRPLEFFPDDDCTSSARREQKREQYRQVREHVRNDDGRLQACGWSLPAKYKQLSPNGGQEDTRMKNVPVPVYCRPLVEKDPSTKLWCAAGVNLSGWKPHEEDSSNGPKPVPGRDPLTCDREGEGEPKSTHPSPEKKKAKETPEADATSSRVWILTSTLTTSKVVIIDANQPGTIVDQFTVCNAHVLCISSIPAASDSDYPPGEMFLDSDVNPEDSGADGVLAGITLVGCATRCNVPRSNCSSRGDTPVLDKGQGDVATTANGKVNPSQSTEEATEATEVPDPGPSESEATTVRPGPLTEHVFTDPAPTPSSSTQPASENGSESNGTIVQPQVEPSGELSTTTSSAAPTMWLGAQNGWLYVHSAVANWKKCLHSIKLKDSVLSLVHVKGRVLVALADGTLAIFHRGEDGQWDLSNYHLMDLGHPHHSIRCMAVVNDRVWCGYKNKVHVIQPKTMQIEKSFDAHPRRESQVRQLAWIGDGVWVSIRLDSTLRLYHAHTHQHLQDVDIEPYVSKMLGTGKLGFSFVRITALLIAGNRLWVGTGNGVVISIPLTETVVLHRGQLLGLRANKTSPTSGEGTRPGGIIHVYGDDSSDKAASSFIPYCSMAQAQLCFHGHRDAVKFFVSVPGNVLATLNGSVLDSPSEGPGPAAPAADAEGQKLKNALVLSGGEGYIDFRIGDGEDDETEECAGDVNQTKPSLSKAERSHIIVWQVSYTPE D7DEE54C17B74106 jip3_drome JNK-interacting protein 3 syd CG8110 Protein sunday driver protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMDNDDALLNNGGPQSGAETVYGTEDNNMVMSEKNEQVVSIVQQLAGSIYQEFERMINRYDEDVVKNLMPLLVNVLECLDASYRINQEQDVEVELLREDNEQLVTQYEREKSARKQSEQKLLEAEDLAEQENKELATRLESVESIVRMLELKHKNSLEHASRLEEREADLKKEYNKLHERYTELFKNHVDYMERTKMLMGSTHSQMSTASERMDVSRARLNPVARSSGPVSYGFASLENSVMLDTETICSVGSQSDDSGPPSLQNELDNLSGTLERGAATDALQQQHHATSPQSPDSSPVVPNVPTNVGRSTTKKEQRSDNNLYQELSFQDNEESEENEIVTGSWVHPGEYASSANDNYFGMGKEVENLIMENNELLATKNALNIVKDDLIVKVDELTGEVEIVREELNAMQQSRTKLRQRISELEDELKKAKEQVKQQNTEQEENDVPLAQRKRFTRVEMAMVLMERNQYKERLMELQEAVRLTEILRASRTVDNLDRKSKQSIWKYFSNLFTPSNRPTERVADGLGGGPMFRHTGGGSPAHSHGSPSRGSGGGDNRLALTSGQPPVHPASAGLANALIMPKDYAEEGSSERISARRREQYRQLRAHVQKEDGRLHAYGWSLPINKASQEANPNRHSGGVPVPVYCNPLAEASPHMKVFCAAGVNLHGGFTKNGQSLIPANSPYAPKSTLKIAEITSPTADQSMEALDRQMARVSLETLEPETQLSSFVWICTSTHAASTVSVVDANQSATVLDAFPICASHLLCIASVQGAMESDYALLEQSEVVKAGEMLQRPGEGTELLGKVEFVRVKPKSDDEQNSNEKQQQEEEEAKEATEKSNEQLPAVSAEEPLGNVEAIKIRQPLPGAPQRLSTDGNQTNNNNNSSSSSNLLFATKSLNPILETKDRDEPAMSSVGPTMWLGAQDGWLYVHSSVGRWHECLHRVLLPDAVLAIVHVEARVVVALANAQLAVFRRQTDGQWDLNSYHLVTLGDRNHSIRCLCVAGERIWAAHRNKIFIVDPVSLNIVHSLDAHPRKESQVRQMAATGAGVWVSIRLDSTLRLYNTHTFEHKQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWIGTSNGVIISVPLAEVQPKSSSDPHGQMPLCCMANAQLSFHGHRDAVKFFVSVPMLQQPNLNGGLTFTNKRPDMLVMCGGEGYIDFRINDNDMENSIQLEPNQTIENRGDKSYLIVWHVSQR 2162C18066A057B8 klc2_mouse Kinesin light chain 2 Klc2 protein protein mouse Mus musculus Mouse MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEMLPQEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREESKDKRRDRRPMEYGSWYKACKVDSPTVNTTLRTLGALYRPEGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGRGHRRGSRDVAGPQSESDLEESGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVRKLQGGGPQEPNSRMKRASSLNFLNKSVEEPVQPGGRVFLTAAL DIP protein O88448 original sequence version: 114 DIP protein O88448 original sequence version: 80 A5E6F9081609E1CE klc_drome Kinesin light chain Klc CG5433 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTQMSQDEIITNTKTVLQGLEALRVEHVSIMNGIAEVQKDNEKSDMLRKNIENIELGLSEAQVMMALTSHLQNIEAEKHKLKTQVRRLHQENAWLRDELANTQQKFQASEQLVAQLEEEKKHLEFMASVKKYDENQEQDDACDKSRTDPVVELFPDEENEDRHNMSPTPPSQFANQTSGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLERTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGCYLKQGRYTEAEILYKQVLTRAHEREFGAIDSKNKPIWQVAEEREEHKFDNRENTPYGEYGGWHKAAKVDSPTVTTTLKNLGALYRRQGMFEAAETLEDCAMRSKKEAYDLAKQTKLSQLLTSNEKRRSKAIKEDLDFSEEKNAKP DIP protein P46824 original sequence version: 100 B7893FF47EE638A9 klc-syd-1 2 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association AA 1-174 of SYD and AA 4-12 and 163-508 (IPR001440) of KLC were sufficient for interaction. Revisit when Protein Editor in place. kns2-mapk8ip3-2 3 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey physical association physical association His-tagged N-terminus of mSYD2 was pulled down by GST-KLC1 Revisit when Protein Editor in place. klc2-mapk8ip3-1 1 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association Antibody used was against AA 1-772 of mSYD2. klc2-mapk8ip3-2 3 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Revisit when Protein Editor in place. N-terminal of mSYD2 was pulled down. mSyd2 was his-tagged and KLC2 was GST-tagged kns2-mapk8ip3-1 1 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association mapk8ip3-klc2-1 1 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association syd-klc-1 2 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association AA 1-724 of SYD were important to the interaction. Revisit when Protein Editor in place. mapk8ip3-kns2-1 1 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role bait bait association association Antibodies against AA 63-90 of KLC used