IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. J. Biol. Chem. (0021-9258) 2000 Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2000 Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. J. Biol. Chem. (0021-9258) 2000 Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. in gel kinase assay in-gel kinase assay in gel kinase assay predetermined predetermined participant predetermined Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2000 Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells. J. Biol. Chem. (0021-9258) 2000 Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. pull down pull down monoclonal western monoclonal antibody western blot monoclonal western Kim YS., Han SJ., Ryu JH., Choi KH., Hong YS., Chung YH., Perrot S., Raibaud A., Brey PT., Lee WJ. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2000 ikkb_drome Inhibitor of nuclear factor kappa-B kinase subunit beta IKKbeta Cactus kinase IKK DIK IKK-like protein Immune response deficient protein 5 Lipopolysaccharide-activated kinase ird5 CG4201 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MITVVFCFSDCHGGIQILGRMSSVNKIKLNENNKMHSFGNWERCRNLGEGGFGLVIHWRNRTTGREIATKHIKEMGALSADQQVKLSERWNKELNWSRQFKNFPHIVAGVDIEDPDFLEYLNGMFSAKLPVIVLEYCNGGDVRKRLQSPENANGLTEFEVRQILGALRKALHFLHSQCGICHRDLKPDNIVIQRGVDGKKIYKLTDFGLARGTPDQTMVQSVVGTRHYYAPEVVENGFYNSTVDLWSFGVIAYELVTGELPFIPHQTLKNIILNLIKKPAKCIAITEDPEDNTRFVNQFELPQTHHLSRPWAAQFTKWLASPLNSNYKERGQLAANNVPVVFADLDKILNMNVLTIFAVNNCERLEYAVSAEMTMKDLIALIVLDTGMDEKELYFVLPTSHPHKTITPKSTPLQLYVEEWSDTSKDSRKWTKRSNPPVMLYIFQVKKECDYKIPEPILSILSRKFIANKFKTKERWLQKRVVLDMLYVLTKEQARYEMLVSGINERALSLEDEMMENSFIDSIDKQRIIISFAYDQLTSLLKEAQAKIPSRQLISSAQWEKLNRNYNFIIQSAKSIRSFLEACLREAKDMVKTTNQLRKEVCEKDLFDCARFYKKYLCNGAIISPSELNNDAEEFAKSRFKLYNEGEARHLPKSIDHMHYLYFKTKESIPVLLQQFCDIKKEIFQINLQMLMSASSTPPPKLELSAAMDRLAISSGSPSSDPFDSLRTINAIEEAERINNILVNEMKIDHY DIP protein Q9VEZ5 original sequence version: 117 0BD2452BED2F8E78 cact_drome NF-kappa-B inhibitor cactus CG5848 cact protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPSPTKAAEAATKATATSDCSCSAASVEQRAPSNAANPSSSLATSGKIGGKTQDQTAAINKQKEFAVPNETSDSGFISGPQSSQIFSEEIVPDSEEQDKDQQESAPQKEQPVVLDSGIIDEEEDQEEQEKEEEHQDTTTATADSMRLKHSADTGIPQWTVESHLVSRGEQLNNLGQSSSTQITGRSKVQSSTASTANANPSGSGATSSAPPSSINIMNAWEQFYQQNDDGDTPLHLACISGSVDVVAALIRMAPHPCLLNIQNDVAQTPLHLAALTAQPNIMRILLLAGAEPTVRDRHGNTALHLSCIAGEKQCVRALTEKFGATEIHEAHRQYGHRSNDKAVSSLSYACLPADLEIRNYDGERCVHLAAEAGHIDILRILVSHGADINAREGKSGRTPLHIAIEGCNEDLANFLLDECEKLNLETATYAGLTAYQFACIMNKSRMQNILEKRGAETVTPPDSDYDSSDIEDLDDTKMYDRFGDPRYFVSYNGGNPMTVA 9E9711B97CDB499F ird5-ird5-1 2 4 enzyme enzyme unspecified role unspecified role drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 4 enzyme target enzyme target substrate substrate unspecified role unspecified role drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. phosphorylation phosphorylation reaction phosphorylation This reaction was LPS dependent. Ird5 was overexpressed in Drosophila Schneider cells. Revisit when OverExpressed in place. Auto-phosphorylation of Ird5. ird5-cact-1 3 4 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey physical association physical association Ird5 is six His tagged and overexpressed in Drosophila schneider cells. Revisit when Protein Editor in place. Revisit when overExpressed in place. ird5-cact-2 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. phosphorylation phosphorylation reaction phosphorylation Cact is phosphorylated. Revisit when overexpressed in place.