MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Threonine 391 phosphorylation of the human prolactin receptor mediates a novel interaction with 14-3-3 proteins. Threonine 391 phosphorylation of the human prolactin receptor mediates a novel interaction with 14-3-3 proteins. J. Biol. Chem. (0021-9258) 2003 imex curation Olayioye MA., Guthridge MA., Stomski FC., Lopez AF., Visvader JE., Lindeman GJ. Source of YWHAZ inferred from previous work of the author (PMID:10477722). The details of other 14-3-3 constructs could not be traced. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Olayioye MA., Guthridge MA., Stomski FC., Lopez AF., Visvader JE., Lindeman GJ. J. Biol. Chem. (0021-9258) 2003 IMEx prlr_human Prolactin receptor PRLR protein protein human Homo sapiens Human MKENVASATVFTLLLFLNTCLLNGQLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMNDTTVWISVAVLSAVICLIIVWAVALKGYSMVTCIFPPVPGPKIKGFDAHLLEKGKSEELLSALGCQDFPPTSDYEDLLVEYLEVDDSEDQHLMSVHSKEHPSQGMKPTYLDPDTDSGRGSCDSPSLLSEKCEEPQANPSTFYDPEVIEKPENPETTHTWDPQCISMEGKIPYFHAGGSKCSTWPLPQPSQHNPRSSYHNITDVCELAVGPAGAPATLLNEAGKDALKSSQTIKSREEGKATQQREVESFHSETDQDTPWLLPQEKTPFGSAKPLDYVEIHKVNKDGALSLLPKQRENSGKPKKPGTPENNKEYAKVSGVMDNNILVLVPDPHAKNVACFEESAKEAPPSLEQNQAEKALANFTATSSKCRLQLGGLDYLDPACFTHSFH DB7FD0328608C787 1433z_human 14-3-3 protein zeta/delta YWHAZ Protein kinase C inhibitor protein 1 protein protein human Homo sapiens Human MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN DIP protein P63104 original sequence version: 144 D464DF2286BBFE60 prlr_mouse Prolactin receptor Prlr protein protein mouse Mus musculus Mouse MSSALAYMLLVLSISLLNGQSPPGKPEIHKCRSPDKETFTCWWNPGSDGGLPTNYSLTYSKEGEKNTYECPDYKTSGPNSCFFSKQYTSIWKIYIITVNATNEMGSSTSDPLYVDVTYIVEPEPPRNLTLEVKQLKDKKTYLWVKWLPPTITDVKTGWFTMEYEIRLKSEEADEWEIHFTGHQTQFKVFDLYPGQKYLVQTRCKPDHGYWSRWGQEKSIEIPNDFTLKDTTVWIIVAVLSAVICLIMVWAVALKGYSMMTCIFPPVPGPKIKGFDTHLLEKGKSEELLSALGCQDFPPTSDCEDLLVEFLEVDDNEDERLMPSHSKEYPGQGVKPTHLDPDSDSGHGSYDSHSLLSEKCEEPQAYPPAFHIPEITEKPENPEANIPPTPNPQNNTPNCHTDTSKSTTWPLPPGQHTRRSPYHSIADVCKLAGSPGDTLDSFLDKAEENVLKLSEDAGEEEVAVQEGAKSFPSDKQNTSWPPLQEKGPIVYAKPPDYVEIHKVNKDGVLSLLPKQRENHQTENPGVPETSKEYAKVSGVTDNNILVLVPDSRAQNTALLEESAKKVPPSLEQNQSEKDLASFTATSSNCRLQLGRLDYLDPTCFMHSFH mint This is a receptor for the anterior pituitary hormone prolactin B8CE202B2EFC9FC6 ywhaz-prlr-1 1 2 unspecified role unspecified role prey prey purified purified region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P16471:p.Thr415Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Wed May 02 18:40:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr415ala' Tue Jun 30 16:21:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr415Ala' P16471:p.Cys413_Thr415delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain CST AAA Fri Jul 22 10:53:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'cys413ala-set414ala-thr415ala' Wed May 02 15:21:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys_ser_thr413-415ala_ala_ala' Tue Jun 30 17:46:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys413_Thr415delinsAlaAlaAla' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. Stoichiometry: 1.0 3 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association Fig. 1B homomint domino mint ywhaz-prlr-2 1 2 unspecified role unspecified role prey prey purified purified human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion Stoichiometry: 1.0 3 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association Fig. 1B homomint domino mint ywhaz-prlr-3 1 2 unspecified role unspecified role prey prey purified purified human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour Stoichiometry: 1.0 3 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association Fig. 3A homomint domino mint ywhaz-prlr-4 1 4 unspecified role unspecified role prey prey purified purified mouse-hc11 Mus musculus hc11 hc11 Stoichiometry: 1.0 3 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association Fig. 3A homomint domino mint