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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>UniProt</shortLabel>
        <fullName>UniProt</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0486" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3989247" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.uniprot.org</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="12504083" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1993010" refType="identity" refTypeAc="MI:0356"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a.</attribute>
            <attribute name="journal" nameAc="MI:0885">Biochem. Biophys. Res. Commun. (0006-291X)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2003</attribute>
            <attribute name="author-list" nameAc="MI:0636">Kaneko C., Hatakeyama S., Matsumoto M., Yada M., Nakayama K., Nakayama KI.</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="12504083" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="9606">
            <names>
              <shortLabel>human</shortLabel>
              <fullName>Homo sapiens</fullName>
              <alias type="synonym" typeAc="MI:1041">Human</alias>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>coip</shortLabel>
            <fullName>coimmunoprecipitation</fullName>
            <alias type="go synonym" typeAc="MI:0303">co-immunoprecipitation</alias>
            <alias type="go synonym" typeAc="MI:0303">CoIp</alias>
            <alias type="go synonym" typeAc="MI:0303">immunoprecipitation</alias>
            <alias type="go synonym" typeAc="MI:0303">Co-IP</alias>
            <alias type="synonym" typeAc="MI:1041">co-immunoprecipitation</alias>
            <alias type="synonym" typeAc="MI:1041">CoIp</alias>
            <alias type="synonym" typeAc="MI:1041">immunoprecipitation</alias>
            <alias type="synonym" typeAc="MI:1041">Co-IP</alias>
            <alias type="synonym" typeAc="MI:1041">coip</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0019" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Kaneko C., Hatakeyama S., Matsumoto M., Yada M., Nakayama K., Nakayama KI.</attribute>
          <attribute name="accepted">Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01).</attribute>
          <attribute name="journal" nameAc="MI:0885">Biochem. Biophys. Res. Commun. (0006-291X)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2003</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>tera_mouse</shortLabel>
          <fullName>Transitional endoplasmic reticulum ATPase</fullName>
          <alias type="gene name" typeAc="MI:0301">Vcp</alias>
          <alias type="gene name synonym" typeAc="MI:0302">15S Mg(2+)-ATPase p97 subunit</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Valosin-containing protein</alias>
        </names>
        <xref>
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          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q6PI18" version="SP_86" refType="secondary-ac" refTypeAc="MI:0360"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:1904949" version="SP_222"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016887" version="SP_222"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019903" version="SP_222"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032510" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032991" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0034098" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0034605" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0035617" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0035800" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0035861" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0036297" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0036435" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0036503" version="SP_222"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019904" version="SP_222"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_033529.3"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-29796N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="10090">
          <names>
            <shortLabel>mouse</shortLabel>
            <fullName>Mus musculus</fullName>
            <alias type="synonym" typeAc="MI:1041">Mouse</alias>
          </names>
        </organism>
        <sequence>MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q01853 original sequence version: 84</attribute>
          <attribute name="remark-internal">DIP protein Q01853 original sequence version: 112</attribute>
          <attribute name="remark-internal">DIP protein Q01853 original sequence version: 152</attribute>
          <attribute name="remark-internal">DIP protein Q01853 original sequence version: 136</attribute>
          <attribute name="remark-internal">DIP protein Q01853 original sequence version: 156</attribute>
          <attribute name="remark-internal">DIP protein Q01853 original sequence version: 149</attribute>
          <attribute name="crc64">501B721D3A77BA8A</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>ube4b_mouse</shortLabel>
          <fullName>Ubiquitin conjugation factor E4 B</fullName>
          <alias type="gene name" typeAc="MI:0301">Ube4b</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Ufd2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Ubiquitin fusion degradation protein 2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Ufd2a</alias>
          <alias type="gene name synonym" typeAc="MI:0302">RING-type E3 ubiquitin transferase E4 B</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q9ES00" version="SP_59" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q6DID4" version="SP_83" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUSP00000099501" version="SP_154" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q9EQE0" version="SP_59" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-80579" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051117" version="SP_154"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051865" version="SP_154"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0061630" version="SP_154"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR003613" version="SP_154"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006457" version="SP_154"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR013083" version="SP_154"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2KR4" version="SP_154"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR019474" version="SP_154"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_071305.2"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="10090">
          <names>
            <shortLabel>mouse</shortLabel>
            <fullName>Mus musculus</fullName>
            <alias type="synonym" typeAc="MI:1041">Mouse</alias>
          </names>
        </organism>
        <sequence>MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCNMGSFSTSSLSSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLESDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAALVNANMKKAQMQADDRLVSTDGFMLNLLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNERLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRILDPAYPDVTLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTVMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH</sequence>
        <attributeList>
          <attribute name="crc64">FC4879E47672E983</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>ube4b-vcp-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-80611" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human-293</shortLabel>
                  <fullName>Homo sapiens transformed primary embryonal kidney cells</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
                <cellType>
                  <names>
                    <shortLabel>293</shortLabel>
                    <fullName>Transformed human primary embryonal kidney cells.</fullName>
                  </names>
                  <xref>
                    <primaryRef db="cabri" dbAc="MI:0246" id="ACC 305" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="IA:0058" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="MINT-1782570" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-307626" refType="identity" refTypeAc="MI:0356"/>
                  </xref>
                  <attributeList>
                    <attribute name="comment" nameAc="MI:0612">The Cell type named 293 is synonymous with HEK-293.</attribute>
                  </attributeList>
                </cellType>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="6">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human-293</shortLabel>
                  <fullName>Homo sapiens transformed primary embryonal kidney cells</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
                <cellType>
                  <names>
                    <shortLabel>293</shortLabel>
                    <fullName>Transformed human primary embryonal kidney cells.</fullName>
                  </names>
                  <xref>
                    <primaryRef db="cabri" dbAc="MI:0246" id="ACC 305" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="IA:0058" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="MINT-1782570" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-307626" refType="identity" refTypeAc="MI:0356"/>
                  </xref>
                  <attributeList>
                    <attribute name="comment" nameAc="MI:0612">The Cell type named 293 is synonymous with HEK-293.</attribute>
                  </attributeList>
                </cellType>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">UBE4B was N-terminal tagged with FLAG epitope</attribute>
          <attribute name="comment" nameAc="MI:0612">VCP was N-terminal tagged with HA</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>