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  <entry>
    <source releaseDate="2022-02-04">
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        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
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      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
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        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
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        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Regulation of human eIF4E by 4E-BP1: binding analysis using surface plasmon resonance.</fullName>
        </names>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Regulation of human eIF4E by 4E-BP1: binding analysis using surface plasmon resonance.</attribute>
            <attribute name="journal" nameAc="MI:0885">IUBMB Life (1521-6543)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2000</attribute>
            <attribute name="author-list" nameAc="MI:0636">Youtani T., Tomoo K., Ishida T., Miyoshi H., Miura K.</attribute>
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        </bibref>
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        <hostOrganismList>
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            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
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          </hostOrganism>
        </hostOrganismList>
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            <shortLabel>spr</shortLabel>
            <fullName>surface plasmon resonance</fullName>
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            <alias type="go synonym" typeAc="MI:0303">BIAcore(r)</alias>
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            <alias type="synonym" typeAc="MI:1041">Optical biosensor</alias>
            <alias type="synonym" typeAc="MI:1041">spr</alias>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
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        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Youtani T., Tomoo K., Ishida T., Miyoshi H., Miura K.</attribute>
          <attribute name="accepted">Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01).</attribute>
          <attribute name="journal" nameAc="MI:0885">IUBMB Life (1521-6543)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2000</attribute>
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      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>4ebp1_human</shortLabel>
          <fullName>Eukaryotic translation initiation factor 4E-binding protein 1</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Phosphorylated heat- and acid-stable protein regulated by insulin 1</alias>
          <alias type="gene name" typeAc="MI:0301">EIF4EBP1</alias>
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            <shortLabel>protein</shortLabel>
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        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
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        </organism>
        <sequence>MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q13541 original sequence version: 96</attribute>
          <attribute name="remark-internal">DIP protein Q13541 original sequence version: 103</attribute>
          <attribute name="remark-internal">DIP protein Q13541 original sequence version: 120</attribute>
          <attribute name="remark-internal">DIP protein Q13541 original sequence version: 113</attribute>
          <attribute name="crc64">1682A6BA74132966</attribute>
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      </interactor>
      <interactor id="3">
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          <shortLabel>if4e_human</shortLabel>
          <fullName>Eukaryotic translation initiation factor 4E</fullName>
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          <alias type="gene name synonym" typeAc="MI:0302">eIF-4F 25 kDa subunit</alias>
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          <alias type="gene name synonym" typeAc="MI:0302">EIF4EL1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">EIF4F</alias>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 164</attribute>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 127</attribute>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 126</attribute>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 135</attribute>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 151</attribute>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 155</attribute>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 160</attribute>
          <attribute name="remark-internal">DIP protein P06730-1 original sequence version: 187</attribute>
          <attribute name="remark-internal">DIP protein P06730 original sequence version: 146</attribute>
          <attribute name="crc64">B869B8DE615E699D</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>eif4e-4ebp1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-74102" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="10772338" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>enzyme</shortLabel>
                <fullName>enzyme</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0501" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-46" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
          <participant id="6">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>enzyme target</shortLabel>
                <fullName>enzyme target</fullName>
                <alias type="go synonym" typeAc="MI:0303">substrate</alias>
                <alias type="synonym" typeAc="MI:1041">substrate</alias>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0502" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-64" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>