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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
      <bibref>
        <xref>
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      </bibref>
      <xref>
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      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it/mint</attribute>
        <attribute name="url" nameAc="MI:0614">url&amp;gt;http://mint.bio.uniroma2.it/mint/</attribute>
        <attribute name="url" nameAc="MI:0614">https://mint.bio.uniroma2.it</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">MINT-[0-9]+</attribute>
        <attribute name="search-url" nameAc="MI:0615">https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac}</attribute>
        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Phosphorylation of sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="9725902" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="mint" dbAc="MI:0471" id="MINT-6548144" refType="primary-reference" refTypeAc="MI:0358"/>
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            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8683595" refType="identity" refTypeAc="MI:0356"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Phosphorylation of sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation.</attribute>
            <attribute name="journal" nameAc="MI:0885">Mol. Biol. Cell (1059-1524)</attribute>
            <attribute name="publication year" nameAc="MI:0886">1998</attribute>
            <attribute name="author-list" nameAc="MI:0636">Nishizawa M., Kawasumi M., Fujino M., Toh-e A.</attribute>
            <attribute name="uniprot-dr-export">no</attribute>
            <attribute name="contact-email" nameAc="MI:0634">mas@mc.med.keio.ac.jp</attribute>
          </attributeList>
        </bibref>
        <xref>
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          <secondaryRef db="pubmed" dbAc="MI:0446" id="9725902" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>pull down</shortLabel>
            <fullName>pull down</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1223" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Nishizawa M., Kawasumi M., Fujino M., Toh-e A.</attribute>
          <attribute name="uniprot-dr-export">no</attribute>
          <attribute name="contact-email" nameAc="MI:0634">mas@mc.med.keio.ac.jp</attribute>
          <attribute name="journal" nameAc="MI:0885">Mol. Biol. Cell (1059-1524)</attribute>
          <attribute name="publication year" nameAc="MI:0886">1998</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>pho85_yeast</shortLabel>
          <fullName>Cyclin-dependent protein kinase PHO85</fullName>
          <alias type="gene name" typeAc="MI:0301">PHO85</alias>
          <alias type="gene name synonym" typeAc="MI:0302">SSG3</alias>
          <alias type="orf name" typeAc="MI:0306">P7102.18A</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Serine/threonine-protein kinase PHO85</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Negative regulator of the PHO system</alias>
          <alias type="locus name" typeAc="MI:0305">YPL031C</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P17157" version="SP_79" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q03089" version="SP_79" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q06888" version="SP_79" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensemblfungi" id="YPL031C" version="SP_205" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="D6W3Y2" version="SP_114" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-13327" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_015294.1"/>
          <secondaryRef db="sgd" dbAc="MI:0484" id="S000005952"/>
          <secondaryRef db="ensemblfungi" id="YPL031C" version="SP_205" refType="gene"/>
          <secondaryRef db="ensemblfungi" id="YPL031C_mRNA" version="SP_205" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000083" version="SP_205"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000122" version="SP_205"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000307" version="SP_205"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0004672" version="SP_205"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005524" version="SP_205"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006468" version="SP_205"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006974" version="SP_205"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016239" version="SP_205"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019216" version="SP_205"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032880" version="SP_205"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0045719" version="SP_205"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0050849" version="SP_205"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2PK9" version="SP_205"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2PMI" version="SP_205"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4KRC" version="SP_205"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:1901987" version="SP_205"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1990860" version="SP_205"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000719" version="SP_205"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008271" version="SP_205"/>
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          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR017441" version="SP_205"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="P17157"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-1493N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="559292">
          <names>
            <shortLabel>yeast</shortLabel>
            <fullName>Saccharomyces cerevisiae</fullName>
            <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
          </names>
        </organism>
        <sequence>MSSSSQFKQLEKLGNGTYATVYKGLNKTTGVYVALKEVKLDSEEGTPSTAIREISLMKELKHENIVRLYDVIHTENKLTLVFEFMDNDLKKYMDSRTVGNTPRGLELNLVKYFQWQLLQGLAFCHENKILHRDLKPQNLLINKRGQLKLGDFGLARAFGIPVNTFSSEVVTLWYRAPDVLMGSRTYSTSIDIWSCGCILAEMITGKPLFPGTNDEEQLKLIFDIMGTPNESLWPSVTKLPKYNPNIQQRPPRDLRQVLQPHTKEPLDGNLMDFLHGLLQLNPDMRLSAKQALHHPWFAEYYHHAS</sequence>
        <attributeList>
          <attribute name="crc64">246A4358870B00EC</attribute>
          <attribute name="crc64">246A4358870B00EC</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>sic1_yeast</shortLabel>
          <fullName>Protein SIC1</fullName>
          <alias type="gene name" typeAc="MI:0301">SIC1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">SDB25</alias>
          <alias type="locus name" typeAc="MI:0305">YLR079W</alias>
          <alias type="orf name" typeAc="MI:0306">L9449.8</alias>
          <alias type="gene name synonym" typeAc="MI:0302">CDK inhibitor p40</alias>
        </names>
        <xref>
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          <secondaryRef db="ensemblfungi" id="YLR079W" version="SP_177" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="ensemblfungi" id="YLR079W" version="SP_177" refType="gene"/>
          <secondaryRef db="ensemblfungi" id="YLR079W_mRNA" version="SP_177" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000079" version="SP_177"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000082" version="SP_177"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005737" version="SP_177"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016242" version="SP_177"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3V7D" version="SP_177"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6G86" version="SP_177"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_013180.1"/>
          <secondaryRef db="sgd" dbAc="MI:0484" id="S000004069"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-6817N"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="P38634"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="559292">
          <names>
            <shortLabel>yeast</shortLabel>
            <fullName>Saccharomyces cerevisiae</fullName>
            <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
          </names>
        </organism>
        <sequence>MTPSTPPRSRGTRYLAQPSGNTSSSALMQGQKTPQKPSQNLVPVTPSTTKSFKNAPLLAPPNSNMGMTSPFNGLTSPQRSPFPKSSVKRTLFQFESHDNGTVREEQEPLGRVNRILFPTQQNVDIDAAEEEEEGEVLLPPSRPTSARQLHLSLERDEFDQTHRKKIIKDVPGTPSDKVITFELAKNWNNNSPKNDARSQESEDEEDIIINPVRVGKNPFASDELVTQEIRNERKRAMLRENPDIEDVITYVNKKGEVVEKRRLTDEEKRRFKPKALFQSRDQEH</sequence>
        <attributeList>
          <attribute name="crc64">7913636340678F78</attribute>
          <attribute name="crc64">7913636340678F78</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>sic1-pho85</shortLabel>
        </names>
        <xref>
          <primaryRef db="mint" dbAc="MI:0471" id="MINT-15805" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8683609" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-1538921" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
          <participant id="6">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-1538924" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>phosphorylation</shortLabel>
            <fullName>phosphorylation reaction</fullName>
            <alias type="synonym" typeAc="MI:1041">phosphorylation</alias>
          </names>
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            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49922" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="resid" dbAc="MI:0248" id="AA0033" refType="see-also" refTypeAc="MI:0361"/>
            <secondaryRef db="resid" dbAc="MI:0248" id="AA0034" refType="see-also" refTypeAc="MI:0361"/>
            <secondaryRef db="resid" dbAc="MI:0248" id="AA0035" refType="see-also" refTypeAc="MI:0361"/>
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            <secondaryRef db="resid" dbAc="MI:0248" id="AA0037" refType="see-also" refTypeAc="MI:0361"/>
            <secondaryRef db="resid" dbAc="MI:0248" id="AA0038" refType="see-also" refTypeAc="MI:0361"/>
            <secondaryRef db="resid" dbAc="MI:0248" id="AA0039" refType="see-also" refTypeAc="MI:0361"/>
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            <secondaryRef db="go" dbAc="MI:0448" id="GO:0016310" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>