IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Lectin-like Intestinal Defensin Inhibits 2019-nCoV Spike binding to ACE2 Lectin-like Intestinal Defensin Inhibits 2019-nCoV Spike binding to ACE2 2020 imex curation Wang C, Wang S, Li D, Zhao X, Han S, Wang T, Zhao G, Chen Y, Chen F, Zhao J, Wang L, Sun W, Huang Y, Su Y, Wei D, Zhao J, Wang J Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review. 10.1101/2020.03.29.013490 wangjunping@tmmu.edu.cn,zhaojh@tmmu.edu.cn,dqwei@sjtu.edu.cn Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Chen Wang Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review. Unreduced HD5 has three intermolecular Cysteine-Cysteine (disulphide) cross linked residues. When HD5 was reduced to a linear peptide, HD5 RED , it failed to bind ACE2. 10.1101/2020.03.29.013490 wangjunping@tmmu.edu.cn,zhaojh@tmmu.edu.cn,dqwei@sjtu.edu.cn Accepted 2020-JUN-12 AT 10:04 BST AT 10:04 BST by ORCHARD 2020 Only protein-protein interactions imex curation imex curation q01523-pro_0000417390 HD5(63-94) Defensin, alpha 5 HD5(20-94) DEFA5 DEF5 protein protein human Homo sapiens Human ATCYCRTGRCATRESLSGVCEISGRLYRLCCR 63 94 8561982B6CE69133 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 ace2-defa5-1 1 4 unspecified role unspecified role bait bait purified purified C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 2 unspecified role unspecified role prey prey pCys3 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys31 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys5 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys20 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys10 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys30 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm chemical synthesis Chemical synthesis (Chemical synthesis) 11 10 12 13 14 15 direct interaction direct interaction 1A, 1C Unreduced DEFA5 HD5 peptide has three intermolecular Cysteine-Cysteine (disulphide) cross linked residues. When HD5 was reduced to a linear peptide, HD5 RED, it failed to bind ACE2 ace2-defa5-2 1 4 unspecified role unspecified role bait bait purified purified C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 3 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 2 inhibitor inhibitor prey prey pCys3 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys31 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys5 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys20 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys10 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys30 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm chemical synthesis Chemical synthesis (Chemical synthesis) 25 24 26 27 28 29 direct interaction direct interaction 1E ACE2 was precoated with HD5 before addition of Spike. ace2-defa5-3 1 4 unspecified role unspecified role bait bait purified purified C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 3 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 1D, 1E s-ace2-23 1 4 unspecified role unspecified role prey prey purified purified C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 3 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp. Figure 1 ace2-s-38 1 3 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 2 unspecified role unspecified role prey prey pCys3 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys31 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys5 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys20 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys10 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm pCys30 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain true prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm chemical synthesis Chemical synthesis (Chemical synthesis) 49 50 51 52 54 53 direct interaction direct interaction 2 A/B HD5 had less of an effect on ACE2-S1 interaction (Figure 2B).