IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus puglisi@stanford.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins puglisi@stanford.edu Accepted 2021-MAR-16 AT 09:23 GMT AT 09:23 GMT by KALPANAP Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus puglisi@stanford.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro biophysical biophysical tag fluorescence tag visualisation by fluorescence tag fluorescence Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins Kd values: if text says "estimated" I have entered under annotation "Kinetics" rather than as a parameter, but have entered values as a "parameter" if NOT stated to have been estimated - although probably the same method(s) were used for all instances. For sequences used for HCV-IRES (5'UTR) see Supp. figure 4B piccy - as easiest to see what the M+M section is getting at. Linking only added when it is unclear as to order of adding reagents- figures 5 and 6. Interactions involving the "43S PIC" (43S preinitiation complex) have not been captured as the composition of the complex is ambiguous and not stated in this publication, nor are any specific references given;it some instances contains the small ribosomal subunit (40S) bound by the initiation factors eIF1, eIF1A, eIF3 complex, and the eIF2-Met-tRNAiMet-GTP ternary complex (eIF2-TC). [see publications by Sokabe, Masaaki and Fraser, Christopher S., for example - pmid:25246524]. Other instances used here for 43S PIC include eIFs 1, 1A, 5, TC-GMPPNP. Also who knows what mol(s) are interacting with what. Zero-mode waveguides (ZMWs) are small metallic apertures patterned on glass substrates that overcome concentration restrictions by optically limiting background excitation. Binding of a fluorescent ligand to an immobilized complex is manifested through a fluorescent pulse of the corresponding spectral color, which is collected with a micromirror structure on the bottom of the cell. puglisi@stanford.edu Accepted 2021-MAR-16 AT 09:23 GMT AT 09:23 GMT by KALPANAP Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus puglisi@stanford.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro tirfs total internal reflection fluorescence spectroscopy tirfs tag fluorescence tag visualisation by fluorescence tag fluorescence Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins puglisi@stanford.edu Accepted 2021-MAR-16 AT 09:23 GMT AT 09:23 GMT by KALPANAP Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Proceedings of the National Academy of Sciences of the United States of America 2021 imex curation Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus puglisi@stanford.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel tag fluorescence tag visualisation by fluorescence tag fluorescence Lapointe CP., Grosely R., Johnson AG., Wang J., Fernández IS., Puglisi JD. Virus - Publications including interactions involving viral proteins puglisi@stanford.edu Accepted 2021-MAR-16 AT 09:23 GMT AT 09:23 GMT by KALPANAP Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation rl37_human 60S ribosomal protein L37 Large ribosomal subunit protein eL37 RPL37 G1.16 protein protein human Homo sapiens Human MTKGTSSFGKRRNKTHTLCRRCGSKAYHLQKSTCGKCGYPAKRKRKYNWSAKAKRRNTTGTGRMRHLKIVYRRFRHGFREGTTPKPKRAAVAASSSS F565A11E983027C9 rs3a_human 40S ribosomal protein S3a RPS3A MFTL FTE1 v-fos transformation effector protein Small ribosomal subunit protein eS1 protein protein human Homo sapiens Human MAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV DIP protein P61247 original sequence version: 37 000037AE195F7A9D rs16_human 40S ribosomal protein S16 RPS16 Small ribosomal subunit protein uS9 protein protein human Homo sapiens Human MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR DIP protein P62249 original sequence version: 115 519BCFB91BB68A15 rs11_human 40S ribosomal protein S11 RPS11 Small ribosomal subunit protein uS17 protein protein human Homo sapiens Human MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF DIP protein P62280 original sequence version: null 9FB75DC1D99614B0 rl39_rl39l_human RPL39 and RPL39L molecule set molecule set human Homo sapiens Human P62891,Q96EH5 rla1_human 60S acidic ribosomal protein P1 RPLP1 RRP1 Large ribosomal subunit protein P1 protein protein human Homo sapiens Human MASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD 4C282AB8DCA079C8 rl40_human Ubiquitin-60S ribosomal protein L40 UBA52 UBCEP2 CEP52 Ubiquitin A-52 residue ribosomal protein fusion product 1 protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK 7BCB602ABEFAD02A rl30_human 60S ribosomal protein L30 RPL30 Large ribosomal subunit protein eL30 protein protein human Homo sapiens Human MVAAKKTKKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK 95186B081E39748C rs18_human 40S ribosomal protein S18 RPS18 D6S218E Ke-3 Small ribosomal subunit protein uS13 protein protein human Homo sapiens Human MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK DIP protein P62269 original sequence version: 125 4DAF0662C3F37F22 rs12_human 40S ribosomal protein S12 RPS12 Small ribosomal subunit protein eS12 protein protein human Homo sapiens Human MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVDDNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK 45AD1274D55FFA25 rl38_human 60S ribosomal protein L38 RPL38 Large ribosomal subunit protein eL38 protein protein human Homo sapiens Human MPRKIEEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKAEKLKQSLPPGLAVKELK FDD9A107BC98DBBA rs26_human 40S ribosomal protein S26 RPS26 Small ribosomal subunit protein eS26 protein protein human Homo sapiens Human MTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEASVFDAYVLPKLYVKLHYCVSCAIHSKVVRNRSREARKDRTPPPRFRPAGAAPRPPPKPM F60DF98F8900D968 rl6_human 60S ribosomal protein L6 RPL6 TXREB1 Tax-responsive enhancer element-binding protein 107 Neoplasm-related protein C140 Large ribosomal subunit protein eL6 protein protein human Homo sapiens Human MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF D24A6168C7D0ECBF wt_hcv ires +0 dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid synthetic construct synthetic construct TGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTAATACGACTCACTATAGGCCAGCCCCCTGATGGGGGCGACACTCCACCATGAATCACTCCCCTGTGAGGAACTACTGTCTTCACGCAGAAAGCGTCTAGCCATGGCGTTAGTATGAGTGTCGTGCAGCCTCCAGGACCCCCCCTCCCGGGAGAGCCATAGTGGTCTGCGGAACCGGTGAGTACACCGGAATTGCCAGGACGACCGGGTCCTTTCTTGGATAAACCCGCTCAATGCCTGGAGATTTGGGCGTGCCCCCGCAAGACTGCTAGCCGAGTAGTGTTGGGTCGCGAAAGGCCTTGTGGTACTGCCTGATAGGGTGCTTGCGAGTGCCCCGGGAGGTCTCGTAGACCGTGCACCATG A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon). rl32_human 60S ribosomal protein L32 RPL32 Large ribosomal subunit protein eL32 PP9932 protein protein human Homo sapiens Human MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE EDEE48446483966E rs15_human 40S ribosomal protein S15 RIG protein RPS15 RIG Small ribosomal subunit protein uS19 protein protein human Homo sapiens Human MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHLRDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK CA2C682302C7B387 rl14_human 60S ribosomal protein L14 RPL14 CAG-ISL 7 Large ribosomal subunit protein eL14 protein protein human Homo sapiens Human MVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEVKKLQKAALLKASPKKAPGTKGTAAAAAAAAAAKVPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPKASGKKA 4C63DE4FC8E687FE rl11_human 60S ribosomal protein L11 RPL11 CLL-associated antigen KW-12 Large ribosomal subunit protein uL5 protein protein human Homo sapiens Human MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK DIP protein P62913 original sequence version: null 26EC965C9239774E rl8_human 60S ribosomal protein L8 RPL8 Large ribosomal subunit protein uL2 protein protein human Homo sapiens Human MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN DIP protein P62917 original sequence version: 107 CE1610449749318B if1ax_human Eukaryotic translation initiation factor 1A, X-chromosomal Eukaryotic translation initiation factor 4C EIF1AX EIF1A EIF4C protein protein human Homo sapiens Human MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCHIRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDTFGPGDDDEIQFDDIGDDDEDIDDI DIP protein P47813 original sequence version: 131 1C4209855B21BFD4 rl27a_human 60S ribosomal protein L27a RPL27A Large ribosomal subunit protein uL15 protein protein human Homo sapiens Human MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA DIP protein P46776 original sequence version: 122 DIP protein P46776 original sequence version: 124 CB5E09C91507798F rl24_human 60S ribosomal protein L24 RPL24 60S ribosomal protein L30 Large ribosomal subunit protein eL24 protein protein human Homo sapiens Human MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQRAITGASLADIMAKRNQKPEVRKAQREQAIRAAKEAKKAKQASKKTAMAAAKAPTKAAPKQKIVKPVKVSAPRVGGKR 1D48EEB7C0652574 rl13a_human 60S ribosomal protein L13a 23 kDa highly basic protein RPL13A Large ribosomal subunit protein uL13 protein protein human Homo sapiens Human MAEVQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGPYHFRAPSRIFWRTVRGMLPHKTKRGQAALDRLKVFDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGRLAHEVGWKYQAVTATLEEKRKEKAKIHYRKKKQLMRLRKQAEKNVEKKIDKYTEVLKTHGLLV 3E80D0AB77A0D406 rl15_human 60S ribosomal protein L15 RPL15 EC45 Large ribosomal subunit protein eL15 TCBAP0781 protein protein human Homo sapiens Human MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR A6E3AD1A76C9506F rs8_human 40S ribosomal protein S8 RPS8 Small ribosomal subunit protein eS8 OK/SW-cl.83 protein protein human Homo sapiens Human MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK 407FDF59171F7422 rl36a_rl36l_human RPL36A and RPL36AL molecule set molecule set human Homo sapiens Human P83881,Q969Q0 rl3_rl3l_human RPL3 and RPL3L molecule set molecule set human Homo sapiens Human Q92901,P39023 rl13_human 60S ribosomal protein L13 RPL13 BBC1 Breast basic conserved protein 1 Large ribosomal subunit protein eL13 OK/SW-cl.46 protein protein human Homo sapiens Human MAPSRNGMVLKPHFHKDWQRRVATWFNQPARKIRRRKARQAKARRIAPRPASGPIRPIVRCPTVRYHTKVRAGRGFSLEELRVAGIHKKVARTIGISVDPRRRNKSTESLQANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPVRNVYKKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK DB9FC57768E6BEDE rla2_human 60S acidic ribosomal protein P2 RPLP2 RPP2 D11S2243E Renal carcinoma antigen NY-REN-44 Large ribosomal subunit protein P2 protein protein human Homo sapiens Human MRYVASYLLAALGGNSSPSAKDIKKILDSVGIEADDDRLNKVISELNGKNIEDVIAQGIGKLASVPAGGAVAVSAAPGSAAPAAGSAPAAAEEKKDEKKEESEESDDDMGFGLFD B5ECFE2510A756BF rla0_human 60S acidic ribosomal protein P0 RPLP0 60S ribosomal protein L10E Large ribosomal subunit protein uL10 protein protein human Homo sapiens Human MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD 255AD25571C51199 rl37a_human 60S ribosomal protein L37a RPL37A Large ribosomal subunit protein eL43 protein protein human Homo sapiens Human MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKTVAGGAWTYNTTSAVTVKSAIRRLKELKDQ 7122AF87B0E24CF6 eif3j_human Eukaryotic translation initiation factor 3 subunit J EIF3J PRO0391 EIF3S1 Eukaryotic translation initiation factor 3 subunit 1 eIF-3-alpha eIF3 p35 protein protein human Homo sapiens Human MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM DIP protein O75822 original sequence version: 82 DIP protein O75822 original sequence version: 130 83624235424445AA rl10_rl10l_human RPL10 and RPL10L molecule set molecule set human Homo sapiens Human Q96L21,P27635 rs23_human 40S ribosomal protein S23 RPS23 Small ribosomal subunit protein uS12 protein protein human Homo sapiens Human MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS 8417A48B8CF0A8E5 rl7a_human 60S ribosomal protein L7a RPL7A SURF-3 SURF3 Surfeit locus protein 3 PLA-X polypeptide Large ribosomal subunit protein eL8 protein protein human Homo sapiens Human MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG DIP protein P62424 original sequence version: 106 54EF43F221D9F704 eif1_human Eukaryotic translation initiation factor 1 Protein translation factor SUI1 homolog Sui1iso1 A121 EIF1 SUI1 protein protein human Homo sapiens Human MSAIQNLHSFDPFADASKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLVEIGLAKDDQLKVHGF DIP protein P41567 original sequence version: 114 A7BC7F9B53FFEB2C rs30_human 40S ribosomal protein S30 FAU Small ribosomal subunit protein eS30 protein protein human Homo sapiens Human KVHGSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS 012AC1FB555B01A4 rrna 5s human ribosomal RNA 5S gene 17 rrna ribosomal rna rRNA rrna human Homo sapiens Human GUCUACGGCCAUACCACCCUGAACGCGCCCGAUCUCGUCUGAUCUCGGAAGCUAAGCAGGGUCGGGCCUGGUUAGUACUUGGAUGGGAGACCGCCUGGGAAUACCGGGUGCUGUAGGCUUU rl5_human 60S ribosomal protein L5 RPL5 Large ribosomal subunit protein uL18 MSTP030 protein protein human Homo sapiens Human MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES DIP protein P46777 original sequence version: 147 DIP protein P46777 original sequence version: 109 07CA9FBA842BD2A3 rl35a_human 60S ribosomal protein L35a RPL35A Cell growth-inhibiting gene 33 protein Large ribosomal subunit protein eL33 GIG33 protein protein human Homo sapiens Human MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI F32E4A26A25E79E8 rl10a_human 60S ribosomal protein L10a RPL10A NEDD6 CSA-19 Neural precursor cell expressed developmentally down-regulated protein 6 Large ribosomal subunit protein uL1 protein protein human Homo sapiens Human MSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKLVKKLAKKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKSTIKFQMKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRALYIKSTMGKPQRLY 38F1A31119A751D3 rs3_human 40S ribosomal protein S3 RPS3 Small ribosomal subunit protein uS3 OK/SW-cl.26 protein protein human Homo sapiens Human MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA DIP protein P23396 original sequence version: 174 6ECBB34A8EE04AAF rs10_human 40S ribosomal protein S10 RPS10 Small ribosomal subunit protein eS10 protein protein human Homo sapiens Human MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ 64106DFCD97AABA3 rs15a_human 40S ribosomal protein S15a RPS15A Small ribosomal subunit protein uS8 OK/SW-cl.82 protein protein human Homo sapiens Human MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF D4AC1E8864E3A184 rs24_human 40S ribosomal protein S24 RPS24 Small ribosomal subunit protein eS24 protein protein human Homo sapiens Human MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLDYAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKKPKE CE9065C0764D60A6 rl26_rl26l_human RPL26 and RPL26L1 molecule set molecule set human Homo sapiens Human Q9UNX3,P61254 p0dtd1-pro_0000449619 Host translation inhibitor nsp1 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG 1 180 rs5_human 40S ribosomal protein S5 RPS5 Small ribosomal subunit protein uS7 protein protein human Homo sapiens Human MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR DFE2FD5AAFCFD894 rs13_human 40S ribosomal protein S13 RPS13 Small ribosomal subunit protein uS15 protein protein human Homo sapiens Human MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 23F94D38F87B8D53 rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 rl36_human 60S ribosomal protein L36 RPL36 Large ribosomal subunit protein eL36 protein protein human Homo sapiens Human MALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFAPYERRAMELLKVSKDKRALKFIKKRVGTHIRAKRKREELSNVLAAMRKAAAKKD FEE8850A62080EB3 rssa_human 40S ribosomal protein SA RPSA LAMBR LAMR1 Laminin receptor 1 37/67 kDa laminin receptor Laminin-binding protein precursor p40 Colon carcinoma laminin-binding protein NEM/1CHD4 Multidrug resistance-associated protein MGr1-Ag 37 kDa laminin receptor precursor 67 kDa laminin receptor Small ribosomal subunit protein uS2 protein protein human Homo sapiens Human MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS DIP protein P08865 original sequence version: 163 C68DDB16B759E79E rs7_human 40S ribosomal protein S7 RPS7 Small ribosomal subunit protein eS7 protein protein human Homo sapiens Human MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL E21089929CC061E9 rs29_human 40S ribosomal protein S29 RPS29 Small ribosomal subunit protein uS14 protein protein human Homo sapiens Human MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD 41325122B493EFF9 rrna18s human ribosomal RNA 18s human 5 rrna ribosomal rna rRNA rrna human Homo sapiens Human UACCUGGUUGAUCCUGCCAGUAGCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUACGCACGGCCGGUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUGGUUCCUUUGGUCGCUCGCUCCUCUCCUACUUGGAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCCGACGGGCGCUGACCCCCUUCGCGGGGGGGAUGCGUGCAUUUAUCAGAUCAAAACCAACCCGGUCAGCCCCUCUCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCUUUGGUGACUCUAGAUAACCUCGGGCCGAUCGCACGCCCCCCGUGGCGGCGACGACCCAUUCGAACGUCUGCCCUAUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGUGACCACGGGUGACGGGGAAUCAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCACUCCCGACCCGGGGAGGUAGUGACGAAAAAUAACAAUACAGGACUCUUUCGAGGCCCUGUAAUUGGAAUGAGUCCACUUUAAAUCCUUUAACGAGGAUCCAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGCUGCAGUUAAAAAGCUCGUAGUUGGAUCUUGGGAGCGGGCGGGCGGUCCGCCGCGAGGCGAGCCACCGCCCGUCCCCGCCCCUUGCCUCUCGGCGCCCCCUCGAUGCUCUUAGCUGAGUGUCCCGCGGGGCCCGAAGCGUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCCCGAGCCGCCUGGAUACCGCAGCUAGGAAUAAUGGAAUAGGACCGCGGUUCUAUUUUGUUGGUUUUCGGAACUGAGGCCAUGAUUAAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGCUAGAGGUGAAAUUCUUGGACCGGCGCAAGACGGACCAGAGCGAAAGCAUUUGCCAAGAAUGUUUUCAUUAAUCAAGAACGAAAGUCGGAGGUUCGAAGACGAUCAGAUACCGUCGUAGUUCCGACCAUAAACGAUGCCGACCGGCGAUGCGGCGGCGUUAUUCCCAUGACCCGCCGGGCAGCUUCCGGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUAUGGUUGCAAAGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGAAACCUCACCCGGCCCGGACACGGACAGGAUUGACAGAUUGAUAGCUCUUUCUCGAUUCCGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGCGAUUUGUCUGGUUAAUUCCGAUAACGAACGAGACUCUGGCAUGCUAACUAGUUACGCGACCCCCGAGCGGUCGGCGUCCCCCAACUUCUUAGAGGGACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACAGGUCUGUGAUGCCCUUAGAUGUCCGGGGCUGCACGCGCGCUACACUGACUGGCUCAGCGUGUGCCUACCCUACGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUGAUGGGGAUCGGGGAUUGCAAUUAUUCCCCAUGAACGAGGAAUUCCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGAUUAAGUCCCUGCCCUUUGUACACACCGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUCGGCCCACGGCCCUGGCGGAGCGCUGAGAAGACGGUCGAACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA rs2_human 40S ribosomal protein S2 RPS2 RPS4 40S ribosomal protein S4 Protein LLRep3 Small ribosomal subunit protein uS5 protein protein human Homo sapiens Human MADDAGAAGGPGGPGGPGMGNRGGFRGGFGSGIRGRGRGRGRGRGRGRGARGGKAEDKEWMPVTKLGRLVKDMKIKSLEEIYLFSLPIKESEIIDFFLGASLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKLSIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDCYTSARGCTATLGNFAKATFDAISKTYSYLTPDLWKETVFTKSPYQEFTDHLVKTHTRVSVQRTQAPAVATT 66C0DB7ED393B036 rl9_human 60S ribosomal protein L9 RPL9 RPL9P7 RPL9P8 RPL9P9 Large ribosomal subunit protein uL6 OK/SW-cl.103 protein protein human Homo sapiens Human MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFPINVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQADE 0249E6CA12F77934 rl34_human 60S ribosomal protein L34 RPL34 Large ribosomal subunit protein eL34 protein protein human Homo sapiens Human MVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQAQSQKAK FC09D00B98CB770C rl18a_human 60S ribosomal protein L18a RPL18A Large ribosomal subunit protein eL20 protein protein human Homo sapiens Human MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF 5E501C6D835BE580 rs9_human 40S ribosomal protein S9 RPS9 Small ribosomal subunit protein uS4 protein protein human Homo sapiens Human MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED E9CE3CBD59524F81 crpv ires+1 CrPV IRES+0 dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid synthetic construct synthetic construct AGCAAAAATGUGATCTTGCTTGTAAATACAATTTTGAGAGGTTAATAAATTACAAGTAGTGCTATTTTTGTATTTAGGTTAGCTATTTAGCTTTACGTTCCAGGATGCCTAGTGGCAGCCCCACAATATCCAGGAAGCCCTCTCTGCGGTTTTTCAGATTAGGTAGTCGAAAAACCTAAGAAATTTACCTT rs4x_rs4y1_rs4y2_human RPS4X, RPS4Y1 and RPS4Y2 molecule set molecule set human Homo sapiens Human P22090,P62701,Q8TD47 rl18_human 60S ribosomal protein L18 RPL18 Large ribosomal subunit protein eL18 protein protein human Homo sapiens Human MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYKN C4E416E7A5329B7C rl35_human 60S ribosomal protein L35 RPL35 Large ribosomal subunit protein uL29 protein protein human Homo sapiens Human MAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVKA 92B7694A97AC725D rl41_human 60S ribosomal protein L41 RPL41 HG12 Large ribosomal subunit protein eL41 protein protein human Homo sapiens Human MRAKWRKKRMRRLKRKRRKMRQRSK DIP protein P62945 original sequence version: 82 9AD629C69ECC039E rs21_human 40S ribosomal protein S21 RPS21 Small ribosomal subunit protein eS21 protein protein human Homo sapiens Human MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSKNF 6794AC1292A06BD3 rs19_human 40S ribosomal protein S19 RPS19 Small ribosomal subunit protein eS19 protein protein human Homo sapiens Human MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYGGRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH 181F2DB898E56E41 rs27_rs27l_human RPS27 and RPS27L molecule set molecule set human Homo sapiens Human Q71UM5,P42677 rl28_human 60S ribosomal protein L28 RPL28 Large ribosomal subunit protein eL28 protein protein human Homo sapiens Human MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS 44DCDE45473D7C7B rs25_human 40S ribosomal protein S25 RPS25 Small ribosomal subunit protein eS25 protein protein human Homo sapiens Human MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA B8E05F04FCF2F1A9 biotin biotin (+)-cis-Hexahydro-2-oxo-1H-thieno[3,4]imidazole-4-valeric acid (3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-valeric acid 5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid Biotin BIOTIN biotina biotine biotinum cis-(+)-Tetrahydro-2-oxothieno[3,4]imidazoline-4-valeric acid cis-Hexahydro-2-oxo-1H-thieno(3,4)imidazole-4-valeric acid cis-Tetrahydro-2-oxothieno(3,4-d)imidazoline-4-valeric acid Coenzyme R D-(+)-biotin D-Biotin vitamin B7 Vitamin H 5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) YBJHBAHKTGYVGT-ZKWXMUAHSA-N YBJHBAHKTGYVGT-ZKWXMUAHSA-N rs27a_human Ubiquitin-40S ribosomal protein S27a RPS27A UBA80 UBCEP1 Ubiquitin carboxyl extension protein 80 protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK 617BC63DF3A904F7 rs17_human 40S ribosomal protein S17 RPS17 Small ribosomal subunit protein eS17 RPS17L protein protein human Homo sapiens Human MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV 299AD605C5401325 rl31_human 60S ribosomal protein L31 RPL31 Large ribosomal subunit protein eL31 protein protein human Homo sapiens Human MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN BA9DBE79B9E1C071 rl21_human 60S ribosomal protein L21 RPL21 Large ribosomal subunit protein eL21 protein protein human Homo sapiens Human MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA C51D0B5E8EB9D69E rs28_human 40S ribosomal protein S28 RPS28 Small ribosomal subunit protein eS28 protein protein human Homo sapiens Human MDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLR 49902FE9376EB74F hcv ires(delta-dii)+0 HCV IRES(Delta-dII) dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid synthetic construct synthetic construct TGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTAATACGACTCACTATAGGCCAGCCCCCTGATGGGGGCGACACTCCACCATGAATCACTCCGGACTTCGGTCCACCCCCCCTCCCGGGAGAGCCATAGTGGTCTGCGGAACCGGTGAGTACACCGGAATTGCCAGGACGACCGGGTCCTTTCTTGGATAAACCCGCTCAATGCCTGGAGATTTGGGCGTGCCCCCGCAAGACTGCTAGCCGAGTAGTGTTGGGTCGCGAAAGGCCTTGTGGTACTGCCTGATAGGGTGCTTGCGAGTGCCCCGGGAGGTCTCGTAGACCGTGCACCATG A synthetic DNA was purchased from IDT that contained a 5' flanking sequence (pUC19 backbone sequence), a T7 promoter ( TAATACGACTCACTATAG ), and the HCV IRES (nts 1-344, including the AUG codon) but with domain 2 replaced. rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK DIP protein P62753 original sequence version: 72 DIP protein P62753 original sequence version: 68 DIP protein P62753 original sequence version: 123 A61E435884E636AE rl23_human 60S ribosomal protein L23 RPL23 60S ribosomal protein L17 Large ribosomal subunit protein uL14 protein protein human Homo sapiens Human MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA DIP protein P62829 original sequence version: 116 807E14139B2EB0B5 rl4_human 60S ribosomal protein L4 RPL4 RPL1 60S ribosomal protein L1 Large ribosomal subunit protein uL4 protein protein human Homo sapiens Human MACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQRALRAPRKKIHRRVLKKNPLKNLRIMLKLNPYAKTMRRNTILRQARNHKLRVDKAAAAAAALQAKSDEKAAVAGKKPVVGKKGKKAAVGVKKQKKPLVGKKAAATKKPAPEKKPAEKKPTTEEKKPAA DIP protein P36578 original sequence version: 154 4785ED31699CD792 rl22_rl22l_human RPL22 and RPL22L1 molecule set molecule set human Homo sapiens Human Q6P5R6,P35268 rl7_human 60S ribosomal protein L7 RPL7 Large ribosomal subunit protein uL30 protein protein human Homo sapiens Human MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN 070EB4C904E6795A rl29_human 60S ribosomal protein L29 Cell surface heparin-binding protein HIP RPL29 Large ribosomal subunit protein eL29 protein protein human Homo sapiens Human MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPKIPKGVSRKLDRLAYIAHPKLGKRARARIAKGLRLCRPKAKAKAKAKDQTKAQAAAPASVPAQAPKRTQAPTKASE 2A0C31984EF4FF95 rs14_human 40S ribosomal protein S14 RPS14 Small ribosomal subunit protein uS11 PRO2640 protein protein human Homo sapiens Human MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL 4F4387F9FADAC278 p0c6x7-pro_0000037309 Host translation inhibitor nsp1 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MESLVLGVNEKTHVQLSLPVLQVRDVLVRGFGDSVEEALSEAREHLKNGTCGLVELEKGVLPQLEQPYVFIKRSDALSTNHGHKVVELVAEMDGIQYGRSGITLGVLVPHVGETPIAYRNVLLRKNGNKGAGGHSYGIDLKSYDLGDELGTDPIEDYEQNWNTKHGSGALRELTRELNGG 1 180 B76E7770809587A7 rl23a_human 60S ribosomal protein L23a RPL23A Large ribosomal subunit protein uL23 protein protein human Homo sapiens Human MAPKAKKEAPAPPKAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII 3980E77B47FAB70E rl17_human 60S ribosomal protein L17 60S ribosomal protein L23 PD-1 RPL17 Large ribosomal subunit protein uL22 protein protein human Homo sapiens Human MVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLQKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKISQKKLKKQKLMARE 2FC595DD74ED1CA0 rl27_human 60S ribosomal protein L27 RPL27 Large ribosomal subunit protein eL27 protein protein human Homo sapiens Human MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 73F4151495029A32 a0a088die1_9betc 2'-O-methyltransferase Helicase Host translation inhibitor nsp1 Leader protein NendoU Non-structural protein 10 Non-structural protein 2 Non-structural protein 3 Non-structural protein 4 Non-structural protein 6 Non-structural protein 7 Non-structural protein 8 Non-structural protein 9 ORF1ab polyprotein Papain-like proteinase RNA-directed RNA polymerase Replicase polyprotein 1ab Uridylate-specific endoribonuclease p65 homolog 3C-like proteinase Growth factor-like peptide Guanine-N7 methyltransferase nsp12 nsp13 nsp14 nsp15 nsp16 nsp5 protein protein Bat Hp-betacoronavirus/Zhejiang2013 Bat Hp-betacoronavirus/Zhejiang2013 MCSKGYELNQKTHFSVELPWIQIGENPIKPWSVTPAEALNTVYAAIAEGKAAFIPPFKGMHKYLPELCVFLVRNHGNPTRPFDLQELAVREGDVNYGRSGETVGAIVPLDGQEPYGTITIQLRRRGKNAGGVYAWTNTRDTVTDDLVHEPIDLYTEDPKGKHAQGNLRKFLTGGNDFHRYVDNYYCTGDGKPLPCIVTLLEKAGHANKPLDDAMDYLRGLNRMGQPCRDHDHERVWEVERDNAPYPEQSLFSISAIKTLGEIPHCVFAPDCAVKVMKPRKVKRGADGFMTKLRRVYNINGFDEVKPMALCALHYCEDCGMEMWCHSNFEEAYCPCGKIYTNTPCTLTSAGYVVEGSVVAMPCDACKSDSDHSNGTFYDSCKYLAEDKAEPGNRENFVYFGGCCFAYVGCRNGNAIWVPRAHSDIGNNHSGIVGADVALLNDDLKAIVAQEVIPVVIPEDFKVNETVVALVAAVSGSVDQFIKNCKGLTAETFKCLLEASGKFRVTVDHVIDGCICVATDSAVTALEPALAFCTAANIMYRTLVATSVVISAHTLDALAQRVRDACKHVHPQAMRLLNTLNEYVDYSTNSTLVLCHDVINLPIVLGDILDRLFCGCKKIVESVTTWLYNFSISTIDTIFDLKAFISLIVRNKYKFVKGKFVLLAKNVSSVVQSVVAYLNNVISAFYQQVQFAGTYVGAFEIHQTLLELGGAIYTRVQVAANSIQDLVLPSTVSKKELTFVDGHAIDTELTSEEVTVLEGIVEQLDTDTNKIAGDSIKGDLVTVNNCVFVRFGDGNLSPATTDCQATKGVFKLKGGNPDKVKTVTFGENEVKEITPYITVNVKYTVHDLLDKILNVRLPSFTVEKGLTLQELTAVVEDAVVTQLEAMRAILLEAGVDIDIDDLIDSPYYMFDNAGDSVIGPVMYFSNQQPVDSDELTTASETEEVVEDAKHAATKRSSQDWLDDSCSEDDCEEEDAVTEAESAADSAPALVVVIEEPKSTVSSVSEEPKFPLPEQCSVETHIVVDDTNSKRLLWSDMVEATCGSATDGNEGTVGEVDTCAVAVNRDNTPSTQSIVTVSPQREVHHYVVTEEKWDEHVCGSCKPCEIPLSVKAKRVNQNSVNCGEKAVVTVNSTTVFNTPVPREVEAKTANEQPKVDSTTVAKVTRTKSGHSQEFKPKGYTKLTDNVYIKCADIVEEARNTKPPILVNAANCKLKHGGGVAGAIRKAVGNSIQEDSDLYIKENGAIPVGGAALLPGYNLATNILHVVGPNITMNEDSSLIVNAYSNLNSFSTVVTPLLSAGIFGMKPIDSLTALLSTCKKTVYVVVNDKTIFDACCSHINNKDVKTPSKPVYMMGEVEVFCSTKVAYCNQDKEFCPGTSTLFKPEQLDEIRLKLPEDFLIGDCHKVDDVYVLCIPDRRKGQDLTGFKGLKQLKKVTAASCLPGQGKFGFTIQDVTPLMTACPDIYLVKTSVDNDVQLLCPTVTYNVKQMLARAKQEKLLMCVNIDNKSFTRNVVHLAKNFQHKPGCQTVDGYEFYCYTEKQPLSSIIDELNALGKDIITTPLGCITHQLSLPESAAKMRALKCKFLAVCTSPGAEKQFCAAFTAASSSTEEELFMADCVSNGSYMDWIVINKADGDTIHFLQRDEDIIDYHTSTNKFSMSGVSGLVFKDVKILKRYLEKTKPMLKEVTVYTTVDGTNVNTKILICSKSFGSQLGTCYIDGEDVTSVFPTKQHEGKIVNITQVDDVTSSQVKEYYGVEDTSFLTRYNSALMHVNRWSFAEVPSTGIFCIKWVANNCYLNAAILMLQQLNVEYETPAVREAAFEMRAGKPQRFVALMIAYLKATIGHTGDAREAVRTMLSHAKVQGERHFVMMCKKCGIRRSQFHGLDAVMCFTTNTQLSDFLSGDEAGCTCGDIVPVYQTHIRVPFLILSTTEPKMQMLNCEDTKFLCANNYMGTLSSGHYTHVFNKTLLYSVNGTRVTKMSETTTAVADAVYKDIKYDTEDQFVTYRLDGVWCHEKNPDLSVYYKSGSDYYTSAPIDLTPTEPLSGAEYDNFHLKLVGTLDDNKVKFVQEFNHMVKYDKTKPTRPVTISFYPEMEGDVVALSADKLQQHFKKGAKFGSKFIVWHTGYKITRDLVKPNMAAMRCITTSKPVTTQNSFQALSVEPLPETKHSEHELATEQKPILKLRTEKPVVTAAVTIIPAPKNADAPVLIESLTTDDALAMYVKGTRDFVVNKANSLSKLFGINTIEQNGVDALNMCYDRLAVFDYVKPFLTVTKTASDVTIKAGKVACSLLQRTVPLLYALVVSYLVPLFTRTKQRVSVKVPMVLTKNTCRSVFGLLMNGVANYFTSPKFKSCVRVFALATCIAAILYMAMFVYLFFDIWLSKANVWTDVQTLKDNFGLGTVCDKFQTAYNNSGIYDATEYCTGKSFMCSLCLRGMDSLNFYTELSTSQKTVSWSSFDFSNINTAAEWCLAYLLYTKMFVVLLATAIFQWFAVYFGLYLTQSSWLMWTMVRVISLVPTSILVRMYIYCASFYFVYRAYVHVVSGCQDTRCLMCYKRNLARRVECHTVVQGSRKSFYVHANGGTGFCEKHAWNCINCDSYGPGNTFICDEVAVDLSTTFKRPVKPTDKSHYFVDSVEVKDTFVHLYYSKNDTRVYDRYPLSVFSSVDFLKLANIKTMPSTNVLVFDASNKTEENTTVAASVYYSQLCCQPMLLIEQKLLSNIGDATEVSKKMLDAYIEKFMSTFGVTMEKLKTFVSAAKNSLNDGVPLEKVLKEFVAAAKQNCAETDVEADSIVNALQLTFKDDVDITKDSYNDFVLTYNKVDSMTPYDLGVCIEVGARHVNANVAKANAAALIWNIDHYLLLSESFRRQIRSSARKNGLQFKLTCSKNRQVTPFLTTKIMFSGGATYTTWLKRLLKVAMVMLLVCCILYCLLPSFKSQPLNMYSDEIIDFKVIEDGSLRDITADDTCFSNKHSSFVSWLSRNGFKMTTTSRHCPITVAVVAKKVGDVIPSLPGVVTRVLVRGQIMHFLARVFSAAANICYTPGTLIEYEDFLTSACVLPAECTIFKDSNGIAVPYCYDVNALQGSLPYSSLKHDVLYQLVDNSFIKFPKVLVEGAFRIVRTMTTQYCRHGVCEDSMAGLCLSLRGSWVLNNPYYTSQSGVYCGNDVVDLLMNILAPMFRPAGGMDITSSIVAGGILSLVITVVAYYILKLRRAFGDYQQVVVVNTLAFLCSFLVLCVSTQYPVLPTVYSILYFYMSLYMANEVSFIMHCSWCVMFVPIVPFWCTAVYITMTCSKHVYWFFQTFCKKNVVVHDATFSSFAEAALSTFMITRDVYVKLRAEAFLSAAQYNRYLSMFNRYKYFSGSMDTESYREAACCHLAKAMKDFSEQGSDILYQPPSCSLASAVLQSGIRKMSCPTGKVERCMVRVTCGTMTLNGLWLDNTVYCPRHVMCTPEELLAPDYDSILLRKATHSFTVQYGTAYLKVVSYKMTGSVLQLGVDQINPETPKYKFVRAKPGATFSVLACYNGMPAGVYQVAMRPNHTIKGSFLNGSCGSVGYTLGYDRVEFCYMHHMELPTGVHTGTDLEGTFYGDFVDRQTSQSAGSDNTLTLNVLAWLYAAVINGERWFIVPQTCALTDFNTAVLKYGYQSLTEDGVAALDPLVAQTGISVQTMCASLKDLLVHGMRGRCILSSPTLEDEFTPFDIVRQCSGVTLQSTVRSVSSKFFQWMLMTALTFTILFLQLWEMSIFSWFAMHATIPVVILLMGVSAFVTMLVKHKHAFTTLFLMPTLAVVFYYNCIYVPEHWLIEMVSKYVDLTDTIQLFYGYSIRELIALFAVVIAMSVLSLRIVYDDNTRRVWLATNVLTWLYRLYAGTTVEKAMAYWALLMSLTTNYSGCVTVIMFAAKGVTTLIYYQFPFLGLLIAEFKFLMLVYTCIGYLCCVYYGIFCMLNKYLRCTFGVYPYLVSTQEFRYMNSQGLTPPTNSWDALKMNIKLSGVGGVPCIKVSNVQSKMSDIKCTSVVLLSVLQQLRIEANSRIWSLCVTLHNDILLAKDATEAFEKLTTLLSVLLSMPGAVDLTKLCDDVFENPAILQAVSNEFNNLPTFVEYECAQKAYDEALANGEGPIVLKQLKKAVNCAKSAFERDAAVARKLERMADLAMTQMYKEARAEDKRARVTSAMQSMLFSMLRRLDNDALNNIITNARDGCVPLNVIPMLASSKLLIVVPDISVYNNTVEGTLLTYAGAVWDVVQVTDADNKPIAMGDITIDTAPTLAWPLVLSAVRRVSATKLQNNELAPQLLNQKAVTGGSEQNICDQPALAYYNTVQGKHVILAVVSDHDGLRWTRVDKSDGNGIVMIELDPPCRFITQTSSGPKVKYLYFIKGLNNLCRGMVLGTVAATVRLQAGKPTEVAANSKCLTFCAFAVDPEKAYFDFVNSGGQPLNNCVRVLCTHTGTGRAITADPEATADQESYGGSSCCLYCRCHISHPSHNGLCKYKGKYVQIPNVCTNDPVGFLLKNTVCNVCACWIGYGCNCDTLRQPVLHAADDSFLNRVRGVSAARLTPCGSGLSTDVVVRAFDLYNSKIGGFGLRYKGNCCRFQEIDEDGKALDSFFIVKRHTEDNFKLEQEMYDLLKDSGVVAVHDFFHFRVEGRMEPHITRQRLTKYTMADLVYAFRHFDENSCEVLKEILVTYNCCGSDYFEKKDWYDYVENPDILNVYARLGERIRCNLLKTVKFCDAMKRHGIVGVLTLDNQDLNGNWCDFGDFVRGFPGNGVPVVDSYYSLLMPLMTMTKMLEAETHVDCDLTKDHVKWDLLQYDYTEHRLNLHQKYFKYWDMPYHPNCQSCPDDRCILHCANFNVLYSTTIPQTSFGPIVKRIYVDGVPFVVSAGYHYKELGVVMNQDIHVHNARLSLRELLVYAADPAMHAASGTLLLDKRTTCFSVASLTNTVSFQTVKPGNFNKDFYDFAVSKGFFKEGSSVDLKHFFFAQDGNAAITDYSYYRYNLPTMCDIKQLLFTMEVVDKYFDCYDGGCLNANQVVVNSLDKSAGFPFNKWGKARLYYESLSYEDQDALFAYTKRNVLPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKMLKSIAATRNVSVVIGTTKFYGGWNNMLKTLYADVDNAQLMGWDYPKCDRAMPNMLRIFASLILARKHNTCCNVSERYYRLANECAQVLSEMVLCGGALYVKPGGTSSGDATTAYANSVFNICQAVTANISALLAANGNKIVDVYIRDLQRKLYANVYRSVHVDYKFVDEYYAFLRKHFSMMILSDDGVVCYNSDYVARGYIAGIKDFKQVLYYQNNVFMSEAKCWVEPDLTKGPHEFCSQHTMLVEHNGEQVYLPYPDPSRILGACCFVDDVVKTDGTIMIERFVALAIDAYPLTKHENPEYKQVFYLLLQYIKKLHEELTGHLLDMYSVMISGDNAQRYWEEDFYEAMYTQSVTLQAVGACVLCNSQTSLRCGDCIRRPFLCCKCCYDHVLSTPHKLVLSVSPYVCNAPGCEVTDVTQLYLGGMSYFCKSHKPPISFPLCANGQVFGLYKNTCVGSINVADFNAIATCDWSSSGDYVLANTTTERLKLFAAETLKAVEETAKQSYGVAVVREVVSDKEVILRWEADKPRPPLNRNYVFTGYRITKNSKTQIGEYTFEKSDYGDSVLYKASTTYKLQVGDYFVLTSHSVLPLSAPTLLPQETYTRIVGLYPTFNIPDDYAGNVVNYQKIGMAKYTTVQGPPGTGKSHLAIGVAVYYPTARIVYTACSHAAVDALCEKAFRTLPISKCSRIVPAKARVECFDKFKVNDGTAQYVFSTINALPETSTDVLVVDEVSMCTNYDLSIINARVRAKHIVYVGDPAQLPAPRTLLTRGSLEPENFNSVCRLMKTLGPDIFLGVCRRCPKEIVDTVSELVYENKLKAHKPESGQCFKMFYKGTVTHDVSSAINRPQVGVVREFLSRNPQWKKAVFISPYNSQNAVASKILGLQTQTVDSSQGSEYDYVIFAQTTDTAHSCNVNRFNVAITRAKKGILCIMSDPDMFERLKFVELAVPSRSQIRLQATEITGLFKDCSKLITGLSPAMAPTYMAVDDKFKTNGGLCVHLPGIEKEVSYRRLISMMGFKLDYSVQDYPRLFITRDEAIKQVRAWVGFDVEGAHATKDAVGTNVPLQLGFSTGINFVATPTGFVDTETGTECCRIFAKPPPGEQFKHLIPLMHRGMPWNIVRARIVQMMSDTLKGVTDRVVLVTWAHGFELTSMQYFCKLGRERTCCMCAERATCYSSVHDNFGCWHHCTGFDYLYNPFIVDVQQWGYSGNLQSNHDNHCSVHSGAHVASSDAIMTRCLAIYDCFVKEVHWDIEYPFIADEEFINKSCRKVQNMVARAALLGDKFGTIHDIGNPKALKCVLDAEVNWKFYDAKPVAGRAHKVETLYYQYEVHKDQFQDGVCMFWNCNVDHYPKNSIVCRFDTRSLSKLNLPGCDGGSLYVNKHAFHTPAFQKSAFTDLQQLPFFFYSDTECDSTGQQVVADINYVPLKSNVCITRCNLGGAVCKKHANEYREYLTCYNAVIEAGFSLWIYKWFNIYNLWSTFTKLQGLENVAYNVVNKGHFDGTQGEIPTAVIGDKVFVKREGVDVCVFTNNTTLPTNVAFELYAKRNVNPVPEVKLLRNIGVDICNGFTLWDYEQQAPVFNSTIGVCKYTDIAAKFSEKNCKPLTILFDARLSGHIDQFCNADNAVLYSDYPIKRLQGSKGPEHCSINGVIVNSTPDGGEKPAINCTFYFYKRENGQLVNVGNSYFTQSRVKSSFEPRTTMERDFLELDMHGFIQRYNLEKYAFEHIVYGDFSHKQIGGLHLLIGLIKRCNESTIDIEEFLTMDSTIHNYFVTDQNTGSSKAVCSVIDLLLDDFVEIIKSQDLTVVSKVVKVFIDFRDFDFMLWCKDGVVQTFYPRLQSSSEWQPGQSMPRLYKIQTMCMEKCELLNYGQPPKLPNGILMNVAKYTQLCQYLNKTTIAVPAKMRVIHFGAGSEKGIAPGSTVLKQWLPKDAILVDNDLNDFASDADVTIIGNCVTFHTETKWDLLVSDMYDCDFKSTTMGENYSKDSFFPYLCGFIKNKLSIGGSVAIKITEHSWSADLYNLMGHFAWWTAFCTNVNASSSEAYLIGVNYVGKEVEKIDGNIMHANYIFWRNYSPFNVSNYSLFDMSKFALKAKGTPVMSLKKEQINEMVLGLVEKGRLLIRDTNSFVLTNDMMVNC 9EAC66B8E06B311E rs20_human 40S ribosomal protein S20 RPS20 Small ribosomal subunit protein uS10 protein protein human Homo sapiens Human MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA DIP protein P60866 original sequence version: 112 8802DF7894ADDF94 model mrna (m+41) model mRNA (M+41) rna ribonucleic acid RNA rna synthetic construct synthetic construct GGUCUCUCUCUCUCUCUCUAUGUUCAAAUAAUCUCUCUCUCUCUCUCUCUCUCUCUCUCUCUC rrna 5.8s human ribosomal RNA 5.8S human 5 rrna ribosomal rna rRNA rrna human Homo sapiens Human GACUCUUAGCGGUGGAUCACUCGGCUCGUGCGUCGAUGAAGAACGCAGCUAGCUGCGAGAAUUAAUGUGAAUUGCAGGACACAUUGAUCAUCGACACUUCGAACGCACUUGCGGCCCCGGGUUCCUCCCGGGGCUACGCCUGUCUGAGCGUCGCUU rl12_human 60S ribosomal protein L12 RPL12 Large ribosomal subunit protein uL11 protein protein human Homo sapiens Human MPPKFDPNEIKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKATGDWKGLRITVKLTIQNRQAQIEVVPSASALIIKALKEPPRDRKKQKNIKHSGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHDIIDDINSGAVECPAS 7EFD783A8ED350F9 rl19_human 60S ribosomal protein L19 RPL19 Large ribosomal subunit protein eL19 protein protein human Homo sapiens Human MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDRHMYHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREERLQAKKEEIIKTLSKEEETKK 4AF506393E526216 rrna 28s human ribosomal RNA 28S gene 5 rrna ribosomal rna rRNA rrna human Homo sapiens Human CGCGACCUCAGAUCAGACGUGGCGACCCGCUGAAUUUAAGCAUAUUAGUCAGCGGAGGAGAAGAAACUAACCAGGAUUCCCUCAGUAACGGCGAGUGAACAGGGAAGAGCCCAGCGCCGAAUCCCCGCCCCGCGGCGGGGCGCGGGACAUGUGGCGUACGGAAGACCCGCUCCCCGGCGCCGCUCGUGGGGGGCCCAAGUCCUUCUGAUCGAGGCCCAGCCCGUGGACGGUGUGAGGCCGGUAGCGGCCCCCGGCGCGCCGGGCCCGGGUCUUCCCGGAGUCGGGUUGCUUGGGAAUGCAGCCCAAAGCGGGUGGUAAACUCCAUCUAAGGCUAAAUACCGGCACGAGACCGAUAGUCAACAAGUACCGUAAGGGAAAGUUGAAAAGAACUUUGAAGAGAGAGUUCAAGAGGGCGUGAAACCGUUAAGAGGUAAACGGGUGGGGUCCGCGCAGUCCGCCCGGAGGAUUCAACCCGGCGGCGGGUCCGGCCGUGUCGGCGGCCCGGCGGAUCUUUCCCGCCCCCCGUUCCUCCCGACCCCUCCACCCGCCCUCCCUUCCCCCGCCGCCCCUCCUCCUCCUCCCCGGAGGGGGCGGGCUCCGGCGGGUGCGGGGGUGGGCGGGCGGGGCCGGGGGUGGGGUCGGCGGGGGACCGUCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCAUUUCCACCGCGGCGGUGCGCCGCGACCGGCUCCGGGACGGCUGGGAAGGCCCGGCGGGGAAGGUGGCUCGGGGGGCCCCGUCCGUCCGUCCGUCCGUCCUCCUCCUCCCCCGUCUCCGCCCCCCGGCCCCGCGUCCUCCCUCGGGAGGGCGCGCGGGUCGGGGCGGCGGCGGCGGCGGCGGUGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACCCCCCCCGAGUGUUACAGCCCCCCCGGCAGCAGCACUCGCCGAAUCCCGGGGCCGAGGGAGCGAGACCCGUCGCCGCGCUCUCCCCCCUCCCGGCGCCCACCCCCGCGGGGAAUCCCCCGCGAGGGGGGUCUCCCCCGCGGGGGCGCGCCGGCGUCUCCUCGUGGGGGGGCCGGGCCACCCCUCCCACGGCGCGACCGCUCUCCCACCCCUCCUCCCCGCGCCCCCGCCCCGGCGACGGGGGGGGUGCCGCGCGCGGGUCGGGGGGCGGGGCGGACUGUCCCCAGUGCGCCCCGGGCGGGUCGCGCCGUCGGGCCCGGGGGAGGUUCUCUCGGGGCCACGCGCGCGUCCCCCGAAGAGGGGGACGGCGGAGCGAGCGCACGGGGUCGGCGGCGACGUCGGCUACCCACCCGACCCGUCUUGAAACACGGACCAAGGAGUCUAACACGUGCGCGAGUCGGGGGCUCGCACGAAAGCCGCCGUGGCGCAAUGAAGGUGAAGGCCGGCGCGCUCGCCGGCCGAGGUGGGAUCCCGAGGCCUCUCCAGUCCGCCGAGGGCGCACCACCGGCCCGUCUCGCCCGCCGCGCCGGGGAGGUGGAGCACGAGCGCACGUGUUAGGACCCGAAAGAUGGUGAACUAUGCCUGGGCAGGGCGAAGCCAGAGGAAACUCUGGUGGAGGUCCGUAGCGGUCCUGACGUGCAAAUCGGUCGUCCGACCUGGGUAUAGGGGCGAAAGACUAAUCGAACCAUCUAGUAGCUGGUUCCCUCCGAAGUUUCCCUCAGGAUAGCUGGCGCUCUCGCAGACCCGACGCACCCCCGCCACGCAGUUUUAUCCGGUAAAGCGAAUGAUUAGAGGUCUUGGGGCCGAAACGAUCUCAACCUAUUCUCAAACUUUAAAUGGGUAAGAAGCCCGGCUCGCUGGCGUGGAGCCGGGCGUGGAAUGCGAGUGCCUAGUGGGCCACUUUUGGUAAGCAGAACUGGCGCUGCGGGAUGAACCGAACGCCGGGUUAAGGCGCCCGAUGCCGACGCUCAUCAGACCCCAGAAAAGGUGUUGGUUGAUAUAGACAGCAGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGAGUGUGUAACAACUCACCUGCCGAAUCAACUAGCCCUGAAAAUGGAUGGCGCUGGAGCGUCGGGCCCAUACCCGGCCGUCGCCGGCAGUCGAGAGUGGACGGGAGCGGCGGGGGCGGCGCGCGCGCGCGCGCGUGUGGUGUGCGUCGGAGGGCGGCGGCGGCGGCGGCGGCGGGGGUGUGGGGUCCUCCCCCGCCCCCCCCCCCACGCCUCCUCCCCUCCUCCCGCCCACGCCCCGCUCCCCGCCCCCGGAGCCCCGCGGACGCUACGCCGCGACGAGUAGGAGGGCCGCUGCGGUGAGCCUUGAAGCCUAGGGCGCGGGCCCGGGUGGAGCCGCCGCAGGUGCAGAUCUUGGUGGUAGUAGCAAAUAUUCAAACGAGAACUUUGAAGGCCGAAGUGGAGAAGGGUUCCAUGUGAACAGCAGUUGAACAUGGGUCAGUCGGUCCUGAGAGAUGGGCGAGCGCCGUUCCGAAGGGACGGGCGAUGGCCUCCGUUGCCCUCGGCCGAUCGAAAGGGAGUCGGGUUCAGAUCCCCGAAUCCGGAGUGGCGGAGAUGGGCGCCGCGAGGCGUCCAGUGCGGUAACGCGACCGAUCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGUUCUCUUUUCUUUGUGAAGGGCAGGGCGCCCUGGAAUGGGUUCGCCCCGAGAGAGGGGCCCGUGCCUUGGAAAGCGUCGCGGUUCCGGCGGCGUCCGGUGAGCUCUCGCUGGCCCUUGAAAAUCCGGGGGAGAGGGUGUAAAUCUCGCGCCGGGCCGUACCCAUAUCCGCAGCAGGUCUCCAAGGUGAACAGCCUCUGGCAUGUUGGAACAAUGUAGGUAAGGGAAGUCGGCAAGCCGGAUCCGUAACUUCGGGAUAAGGAUUGGCUCUAAGGGCUGGGUCGGUCGGGCUGGGGCGCGAAGCGGGGCUGGGCGCGCGCCGCGGCUGGACGAGGCGCCGCCGCCCCCCCCACGCCCGGGGCACCCCCCUCGCGGCCCUCCCCCGCCCCACCCCGCGCGCGCCGCUCGCUCCCUCCCCACCCCGCGCCCUCUCUCUCUCUCUCUCCCCCGCUCCCCGUCCUCCCCCCUCCCCGGGGGAGCGCCGCGUGGGGGCGGCGGCGGGGGGAGAAGGGUCGGGGCGGCAGGGGCCGGCGGCGGCCGCCGCGGGGCCCCGGCGGCGGGGGCACGGUCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACUCUGGACGCGAGCCGGGCCCUUCCCGUGGAUCGCCCCAGCUGCGGCGGGCGUCGCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCCCCCACCCCCACCCCACGUCUCGUCGCGCGCGCGUCCGCUGGGGGCGGGGAGCGGUCGGGCGGCGGCGGCGGUCGGCGGGCGGCGGGGCGGGGCGGUUCGUCCCCCCGCCCUACCCCCCCGGCCCCGUCCGCCCCCCGUUCCCCCCUCCUCCUCGGCGCGCGGCGGCGGCGGCGGCGGCGGGCGGCGGAGGGGCCGCGGGCCGGUCCCCCCCGCCGGGUCCGCCCCCGGGGCCGCGGUUCCGCGCGGCGCCUCGCCUCGGCCGGCGCCUAGCAGCCGACUUAGAACUGGUGCGGACCAGGGGAAUCCGACUGUUUAAUUAAAACAAAGCAUCGCGAAGGCCCGCGGCGGGUGUUGACGCGAUGUGAUUUCUGCCCAGUGCUCUGAAUGUCAAAGUGAAGAAAUUCAAUGAAGCGCGGGUAAACGGCGGGAGUAACUAUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCUAAUUAGUGACGCGCAUGAAUGGAUGAACGAGAUUCCCACUGUCCCUACCUACUAUCCAGCGAAACCACAGCCAAGGGAACGGGCUUGGCGGAAUCAGCGGGGAAAGAAGACCCUGUUGAGCUUGACUCUAGUCUGGCACGGUGAAGAGACAUGAGAGGUGUAGAAUAAGUGGGAGGCCCCCGGCGCCCCCCCGGUGUCCCCGCGAGGGGCCCGGGGCGGGGUCCGCCGGCCCUGCGGGCCGCCGGUGAAAUACCACUACUCUGAUCGUUUUUUCACUGACCCGGUGAGGCGGGGGGGCGAGCCCCGAGGGGCUCUCGCUUCUGGCGCCAAGCGCCCGGCCGCGCGCCGGCCGGGCGCGACCCGCUCCGGGGACAGUGCCAGGUGGGGAGUUUGACUGGGGCGGUACACCUGUCAAACGGUAACGCAGGUGUCCUAAGGCGAGCUCAGGGAGGACAGAAACCUCCCGUGGAGCAGAAGGGCAAAAGCUCGCUUGAUCUUGAUUUUCAGUACGAAUACAGACCGUGAAAGCGGGGCCUCACGAUCCUUCUGACCUUUUGGGUUUUAAGCAGGAGGUGUCAGAAAAGUUACCACAGGGAUAACUGGCUUGUGGCGGCCAAGCGUUCAUAGCGACGUCGCUUUUUGAUCCUUCGAUGUCGGCUCUUCCUAUCAUUGUGAAGCAGAAUUCACCAAGCGUUGGAUUGUUCACCCACUAAUAGGGAACGUGAGCUGGGUUUAGACCGUCGUGAGACAGGUUAGUUUUACCCUACUGAUGAUGUGUUGUUGCCAUGGUAAUCCUGCUCAGUACGAGAGGAACCGCAGGUUCAGACAUUUGGUGUAUGUGCUUGGCUGAGGAGCCAAUGGGGCGAAGCUACCAUCUGUGGGAUUAUGACUGAACGCCUCUAAGUCAGAAUCCCGCCCAGGCGGAACGAUACGGCAGCGCCGCGGAGCCUCGGUUGGCCUCGGAUAGCCGGUCCCCCGCCUGUCCCCGCCGGCGGGCCGCCCCCCCCCUCCACGCGCCCCGCGCGCGCGGGAGGGCGCGUGCCCCGCCGCGCGCCGGGACCGGGGUCCGGUGCGGAGUGCCCUUCGUCCUGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCUCGCCCGUCACGCACCGCACGUUCGUGGGGAACCUGGCGCUAAACCAUUCGUAGACGACCUGCUUCUGGGUCGGGGUUUCGUACGUAGCAGAGCAGCUCCCUCGCUGCGAUCUAUUGAAAGUCAGCCCUCGACACAAGGGUUUGUC 40s_human_1-rep-1 5 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells 52 unspecified role unspecified role neutral component neutral component purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 2 C/D, 3D 40s_human_1-rep-2 5 52 competitor competitor competitor neutral component neutral component purified purified P0DTD1-PRO_0000449619:p.Lys164_His165delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KH AA Mon Mar 01 11:00:17 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys164_His165delinsAlaAla' P0DTD1-PRO_0000449619:p.Arg124_Lys125delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RK AA Mon Mar 01 11:27:11 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg124_Lys125delins AlaAla' ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells 52 unspecified role unspecified role neutral component neutral component purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Supp. figure 2 E/F NSP1(KH/AA mutant) was unable to block the NSP1-40S subunit interaction, whereas either wild-type NSP1 or NSP1(RK/AA) prevented comigration of labeled NSP1 with human 40S subunits. 40s_human_1-eif1-1 5 41 stimulator stimulator neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role neutral component neutral component purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association Supp. figure 2 F/G/I, 3D 40s_human_1-eif1ax-1 5 25 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role neutral component neutral component purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association Supp. figure 2 F/I 40s_human_1-eif3j-1 5 37 inhibitor inhibitor neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role neutral component neutral component purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association Supp. figure 2 F 40s_human_1-40s_human_1-1 5 103 unspecified role unspecified role neutral component neutral component purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 25 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role neutral component neutral component purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association Supp. figure 2 I 40s_human_1-eif1-2 3 41 stimulator stimulator ancillary ancillary purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association 2 B-F, Supp. Figure 3G-K,4C please check the Kon is OK- The estimated equilibrium dissociation constant (KD) of the NSP1 interaction with eIF1–40S subunit complexes was ≤∼10 nM. 40s_human_1-rep-3 3 37 inhibitor inhibitor unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) 41 unspecified role unspecified role ancillary ancillary purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association 2 C 40s_human_1-rep-4 3 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association 2 E/F, 4D, Supp. figure 3K, 4C 40s_human_1-rep-5 3 25 unspecified role unspecified role ancillary ancillary purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association 2 E-F, Supp. figure 3K The 43S preinitiation complex, combined eIF1 and eIF1A and 40S, combined eIF1, eIF1A plus eIF3 minus the "j" subunit and 40S, also bound NSP1. there are no CP entries for the eIF3 nor 43S complexes -43S complex includes eif3 complex +others OR eif5. So I have not added as interactors - no references are given for 43S complex. 40s_human_1-rep-6 3 19 unspecified role unspecified role bait bait purified purified 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain n.402delinsGAGCACGAATCCT mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G GAGCACGAATCCT n.402delinsGAGCACG mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G GAGCACG n.402delinsGAGCACGAATCCTAAACCTCAAAGA mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G GAGCACGAATCCTAAACCTCAAAGA n.402delinsGAGCACGAATCCTAAACCTCAAAGAAAAACCGCCAGAACCATGGAAGAC mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G GAGCACGAATCCTAAACCTCAAAGAAAAACCGCCAGAACCATGGAAGAC human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0DTD1-PRO_0000449619:p.Lys164_His165delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain KH AA Mon Mar 01 10:46:30 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys164_His165delinsAlaAla' ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 synthetic construct synthetic construct physical association physical association 3, 4, Supp. figure 4C/I/J,5B There was an estimated KD of ≤∼1 nM. Inclusion of eIFs used for initiation by the HCV IRES (eIF1, eIF1A, eIF5, eIF3deltaj, and TC-GMPPNP) on HCV+12 further slowed, by eightfold, the NSP1 apparent association rate. Kds of the NSP1 interaction with the IRES–40S subunit complex was increased at least 2,000-fold (KD ≥ 2 uM to 3 uM) by long segments of RNA downstream of the start codon. 40s_human_1-rep-7 3 82 unspecified role unspecified role bait bait purified purified 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain n.339delinsAGCACGAATCCTAAACCTCAAAGAAAAACCGCCAGAACCATGGAAGAC mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G GAGCACGAATCCTAAACCTCAAAGAAAAACCGCCAGAACCATGGAAGAC synthetic construct synthetic construct 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association 3 D-G, (4), Supp. figure 4D/E There was a striking delay in NSP1 association with the 40S–HCV(Delta dII)+0 complex with multiphasic behavior. Figure 4 shows results for a HCV IRES(Delta dII) +48 construct;however no comparison is given in the same figure with regard to the +0 construct, so it is not possible to state it the +48 construct showed reduced binding in this instance. ???the HCV(Delta dII) could be added as a mutation decreasing of the WT sequence, but thought better to add as a distinct entity/separately, as difficult to add +48 construct otherwise. 40s_human_1-rep-8 5 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells 52 unspecified role unspecified role neutral component neutral component purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. figure 3D 40s_human_1-eif1-3 5 41 stimulator stimulator neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role neutral component neutral component purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association Supp. figure 3D 40s_human_1-rep-9 5 52 competitor competitor competitor neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role neutral component neutral component purified purified human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells 52 unspecified role unspecified role neutral component neutral component purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Supp. figure 3D I think this is what is shown here. -no mention of competition however. 40s_human_1-rep-10 3 19 unspecified role unspecified role bait bait purified purified 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 90 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association 3, Supp. figure 4G SARS Nsp1 40s_human_1-a0a088die1_9betc-1 3 19 unspecified role unspecified role bait bait purified purified 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 94 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range nsp1_region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association 3 D-G, Supp. figure 4H Bat-Hp Nsp1 40s_human_1-rep-11 3 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells 96 unspecified role unspecified role bait bait purified purified 3-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct physical association physical association 4 B-D, Supp. figure 5 C/D Pre-formed M+41–40S-Cy5 complexes were tethered to the ZMW imaging surface in the presence of eIF1, eIF1A, eIF3, eIF5, and TC-GMPPNP. Inefficient association of NSP1 with the mRNA–40S subunit complexes was observed. model mrna (m+41)-rep-1 3 52 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells 96 unspecified role unspecified role ancillary ancillary purified purified 3-prime cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct physical association physical association 4 B-D, Supp. figure 5 E-H Pre-formed Cy5-M+41–40S subunit complexes were tethered to the ZMW imaging surface in the presence of eIF1, eIF1A, eIF3, eIF5, and TC-GMPPNP. Inefficient association of NSP1 with the mRNA–40S subunit complexes was observed. 40s_human_1-rep-12 4 41 stimulator stimulator ancillary ancillary purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role prey prey purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association Supp. Figure 3E/F 40s_human_1-rep-13 4 76 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 41 stimulator stimulator ancillary ancillary purified purified ecoli Escherichia coli (strain K12) 52 unspecified role unspecified role prey prey purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 142 human-hap1 Homo sapiens HAP1 fibroblast-like cells derived from KBM-7 hap1 Human HAP1 fibroblast-like cells physical association physical association Supp. Figure 3E 40s_human_1-60s_human-1 2 103 unspecified role unspecified role fluorescence donor fluorescence donor purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 66 unspecified role unspecified role ancillary ancillary 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 243 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range 245 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 5 B/C/E The number of nucleotides downstream of the CCU codon is not stated for this assay, but has assumed to be +1. 40s_human_1-60s_human-2 3 52 unspecified role unspecified role prey prey purified purified cy5.5 cyanine label cyanine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 66 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain n.191delinsTTCACA mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T TTCACA n.191delinsTTCACATTTCAAGATACCGGCGCCATGGAAGACGCCAAAAACATAAAG mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T TTCACATTTCAAGATACCGGCGCCATGGAAGACGCCAAAAACATAAAG whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined synthetic construct synthetic construct 243 unspecified role unspecified role ancillary ancillary purified purified C-terminus cy5 label cy5 label c-term range C-terminal range c-term range c-term range C-terminal range c-term range 245 whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 251 258 262 252 257 261 physical association physical association 5 E/F, Supp.figure 7D Inclusion of 60S subunits to yield 80S–CrPV+1 complexes inhibited NSP1 association, (an NSP1 binding event was seen in ∼30% of traces). This is similar to the inhibition observed on both 80S-CrPV+6 and 80S–CrPV+48 complexes. crpv ires+1-rep-1 3 52 unspecified role unspecified role prey prey purified purified cy5.5 cyanine label cyanine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 66 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined synthetic construct synthetic construct 271 268 physical association physical association 5 E/F, Supp. figure 7 C/D When NSP1 was added to 40S–CrPV+1 complexes, slower and less efficient Cy5.5-NSP1 association relative to that of 40S–HCV+0 complexes was observed. crpv ires+1-rep-2 3 52 unspecified role unspecified role prey prey purified purified cy5.5 cyanine label cyanine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role ancillary ancillary purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 19 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined synthetic construct synthetic construct 277 280 physical association physical association 5 F, Supp. figure 7D wt_hcv ires +0-rep-1 3 52 unspecified role unspecified role prey prey purified purified cy5.5 cyanine label cyanine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 19 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain n.402delinsGAGCACGAATCCTAAACCTCAAAGAAAAACCGCCAGAACCATGGAAGAC mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 284 287 physical association physical association 6 A/B/C/D, Supp. figure 8 Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1 was long lived on the ribosomal subunit, for both HCV and CrPV +0/1 and +48 RNAs. Following initial loss of NSP1 signal, NSP1 readily re-associated with the same 40S–HCV+0 complex but not the 40S–HCV+48 complex. (figure 6D and Supp. figure 8) wt_hcv ires +0-40s_human_1-1 3 52 unspecified role unspecified role prey prey purified purified cy5.5 cyanine label cyanine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 66 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain n.191delinsTTCACATTTCAAGATACCGGCGCCATGGAAGACGCCAAAAACATAAAG mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 297 294 physical association physical association 6 A/C, Supp. figure 8 Preformed NSP1–40S complexes were to to ZMWs with surface-immobilized model mRNAs. Association of the NSP1–40S subunit complex with the tethered RNA had kinetics similar to 40S subunits alone. NSP1 was long lived on the ribosomal subunit. Following initial loss of NSP1 signal, NSP1 readily re-associated with the same 40S–HCV+0 complex but not the 40S–HCV+48 complex. Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1 was long lived on the ribosomal subunit, for both HCV and CrPV +0/1 and +48 RNAs. model mrna (m+41)-40s_human_1-1 3 52 unspecified role unspecified role prey prey purified purified cy5.5 cyanine label cyanine label n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 103 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 96 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 307 304 physical association physical association 6 A/C, Supp. figure 8 Preformed NSP1–40S complexes were added to ZMWs with surface-immobilized model mRNAs. NSP1–40S subunit preinitiation complexes (in the presence of eIF1, eIF1A, eIF5, TC-GMPPNP) corecruited to M+41 model mRNAs had very short lifetimes, rapidly codeparting from the mRNA after its expected slow association. wt_hcv ires +0-40s_human_1-2 3 103 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 19 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct physical association physical association Supp. figure 8 40S complexes were added to ZMWs with surface-immobilized model mRNAs. Association of the 40S subunit complex with the tethered RNA had kinetics similar to association with the NSP1-40S complex. crpv ires+1-40s_human_1-1 3 103 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 66 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain n.191delinsTTCACATTTCAAGATACCGGCGCCATGGAAGACGCCAAAAACATAAAG mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct physical association physical association Supp. figure 8 40S complexes were added to ZMWs with surface-immobilized model mRNAs. Association of the 40S subunit complex with the tethered RNA had kinetics similar to association with the NSP1-40S complex. model mrna (m+41)-40s_human_1-2 3 103 unspecified role unspecified role prey prey purified purified n-terminus cy3 label cy3 label n-term range n-terminal range n-term range n-term range n-terminal range n-term range 131 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 96 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct physical association physical association Supp. figure 8 Preformed 40S complexes (in the presence of eIF1, eIF1A, eIF5, TC-GMPPNP)) were added to ZMWs with surface-immobilized model mRNAs. 60s_human RPLP0:RPLP1:RPLP2:RPL3:RPL3L:RPL4:RPL5:RPL6:RPL7:RPL7A:RPL8:RPL9:RPL10:RPL10A:RPL10L:RPL11:RPL12:RPL13:RPL13A:RPL14:RPL15:RPL17:RPL18:RPL18A:RPL19:RPL21:RPL22:RPL22L1:RPL23:RPL23A:RPL24:RPL26:RPL26L1:RPL27:RPL27A:RPL28:RPL29:RPL30:RPL31:RPL32:RPL34:RPL35:RPL35A:RPL36:RPL36A:RPL36AL:RPL37:RPL37A:RPL38:RPL39:RPL39L:RPL41:UBA52 60S cytosolic large ribosomal subunit LSU 97 unspecified role unspecified role 100 unspecified role unspecified role 43 unspecified role unspecified role 10 unspecified role unspecified role 31 unspecified role unspecified role 32 unspecified role unspecified role 51 unspecified role unspecified role 86 unspecified role unspecified role 38 unspecified role unspecified role 34 unspecified role unspecified role 11 unspecified role unspecified role 35 unspecified role unspecified role 62 unspecified role unspecified role 24 unspecified role unspecified role 40 unspecified role unspecified role RPL36 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 87 unspecified role unspecified role 18 unspecified role unspecified role 44 unspecified role unspecified role 70 unspecified role unspecified role 12 unspecified role unspecified role 85 unspecified role unspecified role RPL18A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL18 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL28 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL14 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role 36 unspecified role unspecified role 6 unspecified role unspecified role 56 unspecified role unspecified role RPL7A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL18A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 45 unspecified role unspecified role RPL13A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 69 unspecified role unspecified role 63 unspecified role unspecified role RPL27 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL30 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role 79 unspecified role unspecified role 13 unspecified role unspecified role RPL34 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL13A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 88 unspecified role unspecified role 74 unspecified role unspecified role RPL4 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role 93 unspecified role unspecified role RPL24 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL34 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role RPL27 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 91 unspecified role unspecified role 84 unspecified role unspecified role 80 unspecified role unspecified role 99 unspecified role unspecified role 64 unspecified role unspecified role RPL36 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL4 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 68 unspecified role unspecified role RPL4 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 92 unspecified role unspecified role 29 unspecified role unspecified role 22 unspecified role unspecified role RPL4 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 unspecified role unspecified role RPL30 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPL35A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 33 unspecified role unspecified role 98 unspecified role unspecified role 23 unspecified role unspecified role 46 unspecified role unspecified role 342 356 348 384 386 349 371 350 390 351 357 383 359 393 362 375 367 363 392 368 377 374 physical association physical association human Homo sapiens Human stable complex stable complex biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Responsible for the catalytic activity of the ribosome, the peptidyltransferase activity required to catalyze peptide bond formation. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) (CPX-5223) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only six of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in the case of RPL3/RPL3L and RPL22/RPL22L1, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. A distinct lateral protuberance called the stalk is located on the large ribosomal subunit and plays a central role in the ribosome-mediated stimulation of translation factor-dependent GTP hydrolysis. The 5 acidic ribosomal P-proteins form the pentameric stalk. Accepted 2020-JAN-28 by ORCHARD 40s_human-1 SSU 40S cytosolic small ribosomal subunit FAU:RACK1:RPSA:RPS2:RPS3:RPS3A:RPS4X:RPS4Y1:RPS4Y2:RPS5:RPS6:RPS7:RPS8:RPS9:RPS10:RPS11:RPS12:RPS13:RPS14:RPS15:RPS15A:RPS16:RPS17:RPS18:RPS19:RPS20:RPS21:RPS23:RPS24:RPS25:RPS26:RPS27:RPS27A:RPS27L:RPS28:RPS29 55 unspecified role unspecified role 57 unspecified role unspecified role 65 unspecified role unspecified role 30 unspecified role unspecified role 58 unspecified role unspecified role 83 unspecified role unspecified role 53 unspecified role unspecified role RPS25 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS28 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS14 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 67 unspecified role unspecified role 7 unspecified role unspecified role RPS14 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 47 unspecified role unspecified role FAU binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS20 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS10 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 59 unspecified role unspecified role 81 unspecified role unspecified role RPS5 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 77 unspecified role unspecified role 73 unspecified role unspecified role 17 unspecified role unspecified role 75 unspecified role unspecified role RPS19 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS5 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 50 unspecified role unspecified role 39 unspecified role unspecified role 71 unspecified role unspecified role 95 unspecified role unspecified role RPS3 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 61 unspecified role unspecified role 72 unspecified role unspecified role RPS16 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS25 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role 78 unspecified role unspecified role 8 unspecified role unspecified role RPS19 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 49 unspecified role unspecified role 21 unspecified role unspecified role 89 unspecified role unspecified role RPS5 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS3A binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 54 unspecified role unspecified role 15 unspecified role unspecified role RPS10 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role 48 unspecified role unspecified role RPS3 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RPS12 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 42 unspecified role unspecified role RPS3 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 60 unspecified role unspecified role physical association physical association human Homo sapiens Human stable complex stable complex biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion Component of the ribosome, the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). Acts as the decoding centre of the ribosome which brings mRNA and aminoacylated transfer (t)RNAs together, with the 16S ribosomal (r)RNA being required for the selection of the cognate tRNA. The human ribosome (80S) has a molecular weight of 4.3 MDa: the large subunit (60S) (CPX-5183) consists of 28S, 5S and 5.8S rRNAs and 47 ribonucleproteins (RPs), while the small subunit (40S) possesses a single 18S rRNA chain and 33 RPs. Unlike yeast, in which the majority of RPs have paralogs due to genome duplications, only two of RPs have paralogs in humans. Some RP paralogs are shown to have different tissue specificity and, as in some case, to be mutual exclusive. Ribosome heterogeneity is not unique to the protein components of the ribosome: it can also occur at the level of rRNA. The rRNAs are encoded by multiple copies of ribosomal DNA (rDNA) on different chromosomes. Distinct rRNA usage has been suggested to correlate with different developmental stages. The core of the 40S subunit is formed by the 18S ribosomal RNA, whilst the ribosomal proteins create binding sites for the mRNA and the tRNA A-site, P-site and E-site. Whilst there is normally considered to be a single copy of each polymer in the mature ribosome, there is evidence that the composition can vary in response to physiological conditions, potentially altering the translational efficiency of distinct mRNAs. Accepted 2020-NOV-23 by ORCHARD