IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nature communications 2020 imex curation Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Markus.Zweckstetter@dzne.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Turbidity was measured at 350 nm UV–Vis. Markus.Zweckstetter@dzne.de Accepted 2020-DEC-10 AT 08:46 GMT AT 08:46 GMT by LPERFETTO Nature communications 2020 Only protein-protein interactions imex curation imex curation Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nature communications 2020 imex curation Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Markus.Zweckstetter@dzne.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro uv-vis ultraviolet-visible spectroscopy uv-vis predetermined predetermined participant predetermined Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Turbidity was measured at 350 nm UV–Vis. Markus.Zweckstetter@dzne.de Accepted 2020-DEC-10 AT 08:46 GMT AT 08:46 GMT by LPERFETTO Nature communications 2020 Only protein-protein interactions imex curation imex curation Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nature communications 2020 imex curation Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Markus.Zweckstetter@dzne.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Turbidity was measured at 350 nm UV–Vis. Markus.Zweckstetter@dzne.de Accepted 2020-DEC-10 AT 08:46 GMT AT 08:46 GMT by LPERFETTO Nature communications 2020 Only protein-protein interactions imex curation imex curation Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nature communications 2020 imex curation Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Markus.Zweckstetter@dzne.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay ms participant Identification by mass spectrometry ms participant Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Markus.Zweckstetter@dzne.de Accepted 2020-DEC-10 AT 08:46 GMT AT 08:46 GMT by LPERFETTO Nature communications 2020 Only protein-protein interactions imex curation imex curation Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates. Nature communications 2020 imex curation Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Markus.Zweckstetter@dzne.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M., Savastano A., Ibáñez de Opakua A., Rankovic M., Zweckstetter M. Virus - Publications including interactions involving viral proteins most figure 5, Supp9. results not added as only colocalisation shown, not interaction. Markus.Zweckstetter@dzne.de Accepted 2020-DEC-10 AT 08:46 GMT AT 08:46 GMT by LPERFETTO Nature communications 2020 Only protein-protein interactions imex curation imex curation poly(uridylic acid) poly(uridylic acid) 5'-Uridylic acid, homopolymer Poly U poly(U) Polyuridylic acid Uracil polynucleotide small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA srpk1_human SRSF protein kinase 1 Serine/arginine-rich protein-specific kinase 1 SFRS protein kinase 1 SRPK1 protein protein human Homo sapiens Human MERKVLALQARKKRTKAKKDKAQRKSETQHRGSAPHSESDLPEQEEEILGSDDDEQEDPNDYCKGGYHLVKIGDLFNGRYHVIRKLGWGHFSTVWLSWDIQGKKFVAMKVVKSAEHYTETALDEIRLLKSVRNSDPNDPNREMVVQLLDDFKISGVNGTHICMVFEVLGHHLLKWIIKSNYQGLPLPCVKKIIQQVLQGLDYLHTKCRIIHTDIKPENILLSVNEQYIRRLAAEATEWQRSGAPPPSGSAVSTAPQPKPADKMSKNKKKKLKKKQKRQAELLEKRMQEIEEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLVNPLEPKNAEKLKVKIADLGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATGDYLFEPHSGEEYTRDEDHIALIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWGLFEVLVEKYEWSQEEAAGFTDFLLPMLELIPEKRATAAECLRHPWLNS DIP protein Q96SB4 original sequence version: 128 900E980FE1C16B9A n-poly(uridylic acid)-1 2 7 unspecified role unspecified role neutral component neutral component purified purified region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined decreasing-ptm ptm decreasing an interaction decreasing-ptm ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1B, 4C, Supplementary Fig. 1C At 50 µM protein concentration, the addition of 1 µM polyU, strongly increased turbidity. n-poly(uridylic acid)-2 1 7 unspecified role unspecified role neutral component neutral component purified purified region alexa label alexa label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined decreasing-ptm ptm decreasing an interaction decreasing-ptm ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1C /(D), 4D n-poly(uridylic acid)-3 5 7 unspecified role unspecified role neutral component neutral component purified purified region alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined decreasing-ptm ptm decreasing an interaction decreasing-ptm ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1E, 4E, Supplementary Fig. 1D Fluorescence did not fully recover (Fig. 1e), i.e., ~60% of NSARS-CoV-2 had transformed into an immobile species. please check that Alexa488 if you think is the label here. n-poly(uridylic acid)-4 3 7 unspecified role unspecified role neutral component neutral component purified purified SR-rich region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Ser188 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 3d, 4b, Supplementary Fig. 4 n-srpk1-3 4 7 enzyme target enzyme target substrate substrate neutral component neutral component purified purified Ser188 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm chemical synthesis Chemical synthesis (Chemical synthesis) 8 enzyme enzyme neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9inse 9inse direct interaction direct interaction Supplementary Fig. 8 Supp. figure S8 shows the full length protein based on M. Wts shown. It is stated that:- " identified masses indicate 4 to 5 sites being phosphorylated";however it is not stated which of the many possible sites these were.