IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry. HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry. Nature metabolism 2020 imex curation Wei C., Wei C., Wei C., Wan L., Yan Q., Wang X., Zhang J., Yang X., Zhang Y., Fan C., Li D., Deng Y., Sun J., Gong J., Yang X., Wang Y., Wang X., Li J., Yang H., Li H., Zhang Z., Wang R., Du P., Zong Y., Yin F., Zhang W., Wang N., Peng Y., Lin H., Feng J., Qin C., Chen W., Gao Q., Zhang R., Cao Y., Zhong H., Wei C., Wei C., Wei C., Wan L., Yan Q., Wang X., Zhang J., Yang X., Zhang Y., Fan C., Li D., Deng Y., Sun J., Gong J., Yang X., Wang Y., Wang X., Li J., Yang H., Li H., Zhang Z., Wang R., Du P., Zong Y., Yin F., Zhang W., Wang N., Peng Y., Lin H., Feng J., Qin C., Chen W., Gao Q., Zhang R., Cao Y., Zhong H. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus was unassigned2618. This needs re-checking as many differences between new + old publication;I have tried to make a note of changes going through. zhangrui@cancerhosp-ln-cmu.com, labs.net@gmail.com, towall@yahoo.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wei C. Virus - Publications including interactions involving viral proteins zhangrui@cancerhosp-ln-cmu.com, labs.net@gmail.com, towall@yahoo.com zhangrui@cancerhosp-ln-cmu.com; labs.net@gmail.com; towall@yahoo.com zzzrrr1234@sina.com, towall@yahoo.com Accepted 2020-OCT-30 AT 15:10 GMT AT 15:10 GMT by MARTA 2020 Only protein-protein interactions imex curation imex curation HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry. HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry. Nature metabolism 2020 imex curation Wei C., Wei C., Wei C., Wan L., Yan Q., Wang X., Zhang J., Yang X., Zhang Y., Fan C., Li D., Deng Y., Sun J., Gong J., Yang X., Wang Y., Wang X., Li J., Yang H., Li H., Zhang Z., Wang R., Du P., Zong Y., Yin F., Zhang W., Wang N., Peng Y., Lin H., Feng J., Qin C., Chen W., Gao Q., Zhang R., Cao Y., Zhong H., Wei C., Wei C., Wei C., Wan L., Yan Q., Wang X., Zhang J., Yang X., Zhang Y., Fan C., Li D., Deng Y., Sun J., Gong J., Yang X., Wang Y., Wang X., Li J., Yang H., Li H., Zhang Z., Wang R., Du P., Zong Y., Yin F., Zhang W., Wang N., Peng Y., Lin H., Feng J., Qin C., Chen W., Gao Q., Zhang R., Cao Y., Zhong H. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus was unassigned2618. This needs re-checking as many differences between new + old publication;I have tried to make a note of changes going through. zhangrui@cancerhosp-ln-cmu.com, labs.net@gmail.com, towall@yahoo.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Wei C. Virus - Publications including interactions involving viral proteins Data were derived from the effect of cholesterol on the fluorescence decay of fluorescently labelled proteins. zhangrui@cancerhosp-ln-cmu.com, labs.net@gmail.com, towall@yahoo.com zhangrui@cancerhosp-ln-cmu.com; labs.net@gmail.com; towall@yahoo.com towall@yahoo.com Accepted 2020-OCT-30 AT 15:10 GMT AT 15:10 GMT by MARTA 2020 Only protein-protein interactions imex curation imex curation HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry. HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry. Nature metabolism 2020 imex curation Wei C., Wei C., Wei C., Wan L., Yan Q., Wang X., Zhang J., Yang X., Zhang Y., Fan C., Li D., Deng Y., Sun J., Gong J., Yang X., Wang Y., Wang X., Li J., Yang H., Li H., Zhang Z., Wang R., Du P., Zong Y., Yin F., Zhang W., Wang N., Peng Y., Lin H., Feng J., Qin C., Chen W., Gao Q., Zhang R., Cao Y., Zhong H., Wei C., Wei C., Wei C., Wan L., Yan Q., Wang X., Zhang J., Yang X., Zhang Y., Fan C., Li D., Deng Y., Sun J., Gong J., Yang X., Wang Y., Wang X., Li J., Yang H., Li H., Zhang Z., Wang R., Du P., Zong Y., Yin F., Zhang W., Wang N., Peng Y., Lin H., Feng J., Qin C., Chen W., Gao Q., Zhang R., Cao Y., Zhong H. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus was unassigned2618. This needs re-checking as many differences between new + old publication;I have tried to make a note of changes going through. zhangrui@cancerhosp-ln-cmu.com, labs.net@gmail.com, towall@yahoo.com 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Wei C. Virus - Publications including interactions involving viral proteins (old comment):-I am assuming this is Fluorescence Spect. as the stated reference (6) has no relevant info that I can see. However there are NO Trp (W) residues in most of the peptides, although a "Y" is present... **** there are only 2 peptides that have a "P" residue in the sequence; the others have a "Y" somewhere. The Pro residue located between the CARC and the CRAC was replaced with Trp conferring intrinsic fluorescence. zhangrui@cancerhosp-ln-cmu.com, labs.net@gmail.com, towall@yahoo.com zhangrui@cancerhosp-ln-cmu.com; labs.net@gmail.com; towall@yahoo.com zzzrrr1234@sina.com, towall@yahoo.com Accepted 2020-OCT-30 AT 15:10 GMT AT 15:10 GMT by MARTA 2020 Only protein-protein interactions imex curation imex curation cholesterol cholesterol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT cholesterol-s-1 2 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg683Ala;Arg685Ala;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Sun Dec 13 18:37:33 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro;Arg683Ala;Arg685Ala]' fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range NT-647 red dye fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 1 B/C the supplier of Spike has changed as have tags, parameters + expression system. Also extra interaction for the S1 region protein. cholesterol-s-2 3 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.Pro139Trp mutation mutation certain certain sequence position certain certain certain sequence position certain P W P0DTC2:p.Pro272Trp mutation mutation certain certain sequence position certain certain certain sequence position certain P W Sun Dec 13 19:31:49 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:Pro272Trp' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain lys,lys,lys tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range lys,lys,lys tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1 F s-ace2-36 1 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Extended Data Fig. 5E cholesterol-s-3 2 6 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg683Ala;Arg685Ala;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Sun Dec 13 19:16:42 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro;Arg683Ala;Arg685Ala]' fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range NT-647 red dye fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 competitor competitor competitor neutral component neutral component purified purified P0DTC2:p.[Arg683Ala;Arg685Ala;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain KV PP certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Sun Dec 13 19:10:40 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro;Arg683Ala;Arg685Ala]' fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Extended Data Fig. 1A cholesterol-s-4 2 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range NT-647 red dye fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 1B, number 2 cholesterol-s-5 2 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range NT-647 red dye fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Extended data 1D