IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. Journal of virology 2020 imex curation Yousef M.O. Alhammad,, Alhammad YMO., Kashipathy MM., Roy A., Gagné JP., McDonald P., Gao P., Nonfoux L., Battaile KP., Johnson DK., Holmstrom ED., Poirier GG., Lovell S., Fehr AR. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus results from figure 11 not added as the method makes no sense; they use a "binding reagent" rather than an antibody, Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Yousef M.O. Alhammad, Virus - Publications including interactions involving viral proteins Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Accepted 2020-JUN-12 AT 10:29 BST AT 10:29 BST by ORCHARD 2020 Only protein-protein interactions imex curation imex curation The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. Journal of virology 2020 imex curation Yousef M.O. Alhammad,, Alhammad YMO., Kashipathy MM., Roy A., Gagné JP., McDonald P., Gao P., Nonfoux L., Battaile KP., Johnson DK., Holmstrom ED., Poirier GG., Lovell S., Fehr AR. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus results from figure 11 not added as the method makes no sense; they use a "binding reagent" rather than an antibody, Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Only protein-protein interactions imex curation in vitro In vitro de-adp-ribosylation assay de-ADP-ribosylation assay western blot western blot Immuno blot Immuno blot Yousef M.O. Alhammad, Virus - Publications including interactions involving viral proteins This is assay is for Protein de-ADP-ribosylation (ADP-ribose removal) rather than ADP-ribose addition. In figure 8 a "binding reagent" rather an antibody was used to detect mono-ADP-ribosylated proteins. The binding reagent is an unknown protein with His and Fc tags attached. The Fc tag allows visualization of the binding with conjugated anti-Fc species specific secondary antibodies. Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Accepted 2020-JUN-12 AT 10:29 BST AT 10:29 BST by ORCHARD 2020 Only protein-protein interactions imex curation imex curation The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. Journal of virology 2020 imex curation Yousef M.O. Alhammad,, Alhammad YMO., Kashipathy MM., Roy A., Gagné JP., McDonald P., Gao P., Nonfoux L., Battaile KP., Johnson DK., Holmstrom ED., Poirier GG., Lovell S., Fehr AR. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus results from figure 11 not added as the method makes no sense; they use a "binding reagent" rather than an antibody, Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Only protein-protein interactions imex curation in vitro In vitro de-adp-ribosylation assay de-ADP-ribosylation assay elisa enzyme linked immunosorbent assay ELISA ELISA elisa Yousef M.O. Alhammad,, Alhammad YMO., Kashipathy MM., Roy A., Gagné JP., McDonald P., Gao P., Nonfoux L., Battaile KP., Johnson DK., Holmstrom ED., Poirier GG., Lovell S., Fehr AR. Virus - Publications including interactions involving viral proteins A "binding reagent" rather an antibody was used to detect mono-ADP-ribosylated proteins. The binding reagent is an unknown protein with His and Fc tags attached. The Fc tag allows visualization of the binding, in this instance using HRP conjugated anti-Fc species specific secondary antibodies. Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Accepted 2020-JUN-12 AT 10:29 BST AT 10:29 BST by ORCHARD Journal of virology 2020 Only protein-protein interactions imex curation imex curation The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. Journal of virology 2020 imex curation Yousef M.O. Alhammad,, Alhammad YMO., Kashipathy MM., Roy A., Gagné JP., McDonald P., Gao P., Nonfoux L., Battaile KP., Johnson DK., Holmstrom ED., Poirier GG., Lovell S., Fehr AR. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus results from figure 11 not added as the method makes no sense; they use a "binding reagent" rather than an antibody, Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yousef M.O. Alhammad, Virus - Publications including interactions involving viral proteins Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Accepted 2020-JUN-12 AT 10:29 BST AT 10:29 BST by ORCHARD 2020 Only protein-protein interactions imex curation imex curation The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase. Journal of virology 2020 imex curation Yousef M.O. Alhammad,, Alhammad YMO., Kashipathy MM., Roy A., Gagné JP., McDonald P., Gao P., Nonfoux L., Battaile KP., Johnson DK., Holmstrom ED., Poirier GG., Lovell S., Fehr AR. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus results from figure 11 not added as the method makes no sense; they use a "binding reagent" rather than an antibody, Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Only protein-protein interactions imex curation in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Yousef M.O. Alhammad, Virus - Publications including interactions involving viral proteins Mdo2 was determined to refer to the protein A1Z1Q3 via Ref.30 (pmid:23474712) and the linked PDB entry 4iqy. arfehr@ku.edu Accepted 2020-JUN-12 AT 10:29 BST AT 10:29 BST by ORCHARD 2020 Only protein-protein interactions imex curation imex curation k9n638-pro_0000422456 Papain-like proteinase ORF1a polyprotein 1a protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC APVKKVAFGGDQVHEVAAVRSVTVEYNIHAVLDTLLASSSLRTFVVDKSLSIEEFADVVKEQVSDLLVKLLRGMPIPDFDLDDFIDAPCYCFNAEGDASWSSTMIFSLHPVECDEECSEVEASDLEEGESECISETSTEQVDVSHEISDDEWAAAVDEAFPLDEAEDVTESVQEEAQPVEVPVEDIAQVVIADTLQETPVVSDTVEVPPQVVKLPSEPQTIQPEVKEVAPVYEADTEQTQSVTVKPKRLRKKRNVDPLSNFEHKVITECVTIVLGDAIQVAKCYGESVLVNAANTHLKHGGGIAGAINAASKGAVQKESDEYILAKGPLQVGDSVLLQGHSLAKNILHVVGPDARAKQDVSLLSKCYKAMNAYPLVVTPLVSAGIFGVKPAVSFDYLIREAKTRVLVVVNSQDVYKSLTIVDIPQSLTFSYDGLRGAIRKAKDYGFTVFVCTDNSANTKVLRNKGVDYTKKFLTVDGVQYYCYTSKDTLDDILQQANKSVGIISMPLGYVSHGLDLIQAGSVVRRVNVPYVCLLANKEQEAILMSEDVKLNPSEDFIKHVRTNGGYNSWHLVEGELLVQDLRLNKLLHWSDQTICYKDSVFYVVKNSTAFPFETLSACRAYLDSRTTQQLTIEVLVTVDGVNFRTVVLNNKNTYRSQLGCVFFNGADISDTIPDEKQNGHSLYLADNLTADETKALKELYGPVDPTFLHRFYSLKAAVHKWKMVVCDKVRSLKLSDNNCYLNAVIMTLDLLKDIKFVIPALQHAFMKHKGGDSTDFIALIMAYGNCTFGAPDDASRLLHTVLAKAELCCSARMVWREWCNVCGIKDVVLQGLKACCYVGVQTVEDLRARMTYVCQCGGERHRQIVEHTTPWLLLSGTPNEKLVTTSTAPDFVAFNVFQGIETAVGHYVHARLKGGLILKFDSGTVSKTSDWKCKVTDVLFPGQKYSSDCNVVRYSLDGNFRTEVDPDLSAFYVKDGKYFTSEPPVTYSPATILAGSVYTNSCLVSSDGQPGGDAISLSFNNLLGFDSSKPVTKKYTYSFLPKEDGDVLLAEFDTYDPIYKNGAMYKGKPILWVNKASYDTNLNKFNRASLRQIFDVAPIELENKFTPLSVESTPVEPPTVDVVALQQEMTIVKCKGLNKPFVKDNVSFVADDSGTPVVEYLSKEDLHTLYVDPKYQVIVLKDNVLSSMLRLHTVESGDINVVAASGSLTRKVKLLFRASFYFKEFATRTFTATTAVGSCIKSVVRHLGVTKGILTGCFSFVKMLFMLPLAYFSDSKLGTTEVKVSALKTAGVVTGNVVKQCCTAAVDLSMDKLRRVDWKSTLRLLLMLCTTMVLLSSVYHLYVFNQVLSSDVMFEDAQGLKKFYKEVRAYLGISSACDGLASAYRANSFDVPTFCANRSAMCNWCLISQDSITHYPALKMVQTHLSHYVLNIDWLWFAFETGLAYMLYTSAFNWLLLAGTLHYFFAQTSIFVDWRSYNYAVSSAFWLFTHIPMAGLVRMYNLLACLWLLRKFYQHVINGCKDTACLLCYKRNRLTRVEASTVVCGGKRTFYITANGGISFCRRHNWNCVDCDTAGVGNTFICEEVANDLTTALRRPINATDRSHYYVDSVTVKETVVQFNYRRDGQPFYERFPLCAFTNLDKLKFKEVCKTTTGIPEYNFIIYDSSDRGQESLARSACVYYSQVLCKSILLVDSSLVTSVGDSSEIATKMFDSFVNSFVSLYNVTRDKLEKLISTARDGVRRGDNFHSVLTTFIDAARGPAGVESDVETNEIVDSVQYAHKHDIQITNESYNNYVPSYVKPDSVSTSDLGSLIDCNAASVNQIVLRNSNGACIWNAAAYMKLSDALKRQIRIACRKCNLAFRLTTSKLRANDNILSVRFTANKIVGG 854 2740 532E0D4BDE146A6E p0c6u8-pro_0000338257 Papain-like protease nsp3 ORF1a polyprotein 1a protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus APIKGVTFGEDTVWEVQGYKNVRITFELDERVDKVLNEKCSVYTVESGTEVTEFACVVAEAVVKTLQPVSDLLTNMGIDLDEWSVATFYLFDDAGEENFSSRMYCSFYPPDEEEEDDAECEEEEIDETCEHEYGTEDDYQGLPLEFGASAETVRVEEEEEEDWLDDTTEQSEIEPEPEPTPEEPVNQFTGYLKLTDNVAIKCVDIVKEAQSANPMVIVNAANIHLKHGGGVAGALNKATNGAMQKESDDYIKLNGPLTVGGSCLLSGHNLAKKCLHVVGPNLNAGEDIQLLKAAYENFNSQDILLAPLLSAGIFGAKPLQSLQVCVQTVRTQVYIAVNDKALYEQVVMDYLDNLKPRVEAPKQEEPPNTEDSKTEEKSVVQKPVDVKPKIKACIDEVTTTLEETKFLTNKLLLFADINGKLYHDSQNMLRGEDMSFLEKDAPYMVGDVITSGDITCVVIPSKKAGGTTEMLSRALKKVPVDEYITTYPGQGCAGYTLEEAKTALKKCKSAFYVLPSEAPNAKEEILGTVSWNLREMLAHAEETRKLMPICMDVRAIMATIQRKYKGIKIQEGIVDYGVRFFFYTSKEPVASIITKLNSLNEPLVTMPIGYVTHGFNLEEAARCMRSLKAPAVVSVSSPDAVTTYNGYLTSSSKTSEEHFVETVSLAGSYRDWSYSGQRTELGVEFLKRGDKIVYHTLESPVEFHLDGEVLSLDKLKSLLSLREVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIKPVSYKLDGVTYTEIEPKLDGYYKKDNAYYTEQPIDLVPTQPLPNASFDNFKLTCSNTKFADDLNQMTGFTKPASRELSVTFFPDLNGDVVAIDYRHYSASFKKGAKLLHKPIVWHINQATTKTTFKPNTWCLRCLWSTKPVDTSNSFEVLAVEDTQGMDNLACESQQPTSEEVVENPTIQKEVIECDVKTTEVVGNVILKPSDEGVKVTQELGHEDLMAAYVENTSITIKKPNELSLALGLKTIATHGIAAINSVPWSKILAYVKPFLGQAAITTSNCAKRLAQRVFNNYMPYVFTLLFQLCTFTKSTNSRIRASLPTTIAKNSVKSVAKLCLDAGINYVKSPKFSKLFTIAMWLLLLSICLGSLICVTAAFGVLLSNFGAPSYCNGVRELYLNSSNVTTMDFCEGSFPCSICLSGLDSLDSYPALETIQVTISSYKLDLTILGLAAEWVLAYMLFTKFFYLLGLSAIMQVFFGYFASHFISNSWLMWFIISIVQMAPVSAMVRMYIFFASFYYIWKSYVHIMDGCTSSTCMMCYKRNRATRVECTTIVNGMKRSFYVYANGGRGFCKTHNWNCLNCDTFCTGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVAVKNGALHLYFDKAGQKTYERHPLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCDESASKSASVYYSQLMCQPILLLDQALVSDVGDSTEVSVKMFDAYVDTFSATFSVPMEKLKALVATAHSELAKGVALDGVLSTFVSAARQGVVDTDVDTKDVIECLKLSHHSDLEVTGDSCNNFMLTYNKVENMTPRDLGACIDCNARHINAQVAKSHNVSLIWNVKDYMSLSEQLRKQIRSAAKKNNIPFRLTCATTRQVVNVITTKISLKGG 819 2740 BBB1A6190358ADDE macd2_human ADP-ribose glycohydrolase MACROD2 MACROD2 C20orf133 O-acetyl-ADP-ribose deacetylase MACROD2 [Protein ADP-ribosylaspartate] hydrolase MACROD2 [Protein ADP-ribosylglutamate] hydrolase MACROD2 MACRO domain-containing protein 2 protein protein human Homo sapiens Human MYPSNKKKKVWREEKERLLKMTLEERRKEYLRDYIPLNSILSWKEEMKGKGQNDEENTQETSQVKKSLTEKVSLYRGDITLLEVDAIVNAANASLLGGGGVDGCIHRAAGPCLLAECRNLNGCDTGHAKITCGYDLPAKYVIHTVGPIARGHINGSHKEDLANCYKSSLKLVKENNIRSVAFPCISTGIYGFPNEPAAVIALNTIKEWLAKNHHEVDRIIFCVFLEVDFKIYKKKMNEFFSVDDNNEEEEDVEMKEDSDENGPEEKQSVEEMEEQSQDADGVNTVTVPGPASEEAVEDCKDEDFAKDENITKGGEVTDHSVRDQDHPDGQENDSTKNEIKIETESQSSYMETEELSSNQEDAVIVEQPEVIPLTEDQEEKEGEKAPGEDTPRMPGKSEGSSDLENTPGPDAGAQDEAKEQRNGTK 2CB614734EDB6314 par10_human Protein mono-ADP-ribosyltransferase PARP10 Poly [ADP-ribose] polymerase 10 ADP-ribosyltransferase diphtheria toxin-like 10 PARP10 protein protein human Homo sapiens Human MVAMAEAEAGVAVEVRGLPPAVPDELLTLYFENRRRSGGGPVLSWQRLGCGGVLTFREPADAERVLAQADHELHGAQLSLRPAPPRAPARLLLQGLPPGTTPQRLEQHVQALLRASGLPVQPCCALASPRPDRALVQLPKPLSEADVRVLEEQAQNLGLEGTLVSLARVPQARAVRVVGDGASVDLLLLELYLENERRSGGGPLEDLQRLPGPLGTVASFQQWQVAERVLQQEHRLQGSELSLVPHYDILEPEELAENTSGGDHPSTQGPRATKHALLRTGGLVTALQGAGTVTMGSGEEPGQSGASLRTGPMVQGRGIMTTGSGQEPGQSGTSLRTGPMGSLGQAEQVSSMPMGSLEHEGLVSLRPVGLQEQEGPMSLGPVGSAGPVETSKGLLGQEGLVEIAMDSPEQEGLVGPMEITMGSLEKAGPVSPGCVKLAGQEGLVEMVLLMEPGAMRFLQLYHEDLLAGLGDVALLPLEGPDMTGFRLCGAQASCQAAEEFLRSLLGSISCHVLCLEHPGSARFLLGPEGQHLLQGLEAQFQCVFGTERLATATLDTGLEEVDPTEALPVLPGNAHTLWTPDSTGGDQEDVSLEEVRELLATLEGLDLDGEDWLPRELEEEGPQEQPEEEVTPGHEEEEPVAPSTVAPRWLEEEAALQLALHRSLEPQGQVAEQEEAAALRQALTLSLLEQPPLEAEEPPDGGTDGKAQLVVHSAFEQDVEELDRALRAALEVHVQEETVGPWRRTLPAELRARLERCHGVSVALRGDCTILRGFGAHPARAARHLVALLAGPWDQSLAFPLAASGPTLAGQTLKGPWNNLERLAENTGEFQEVVRAFYDTLDAARSSIRVVRVERVSHPLLQQQYELYRERLLQRCERRPVEQVLYHGTTAPAVPDICAHGFNRSFCGRNATVYGKGVYFARRASLSVQDRYSPPNADGHKAVFVARVLTGDYGQGRRGLRAPPLRGPGHVLLRYDSAVDCICQPSIFVIFHDTQALPTHLITCEHVPRASPDDPSGLPGRSPDT AC9CCFCF9B83A989 adp-d-ribose ADP-D-ribose (Rib5)ppA 5-(adenosine 5'-pyrophosphoryl)-D-ribose A5'pp5Rib adenosine 5'-(trihydrogen diphosphate), P'-5-ester with D-ribose adenosine 5'-diphosphoribose adenosine 5'-pyrophosphate, 5'-5-ester with D-ribofuranose adenosine diphosphate ribose Adenosine diphosphate ribose AdoPPRib ADP ribose ADP-D-ribose ADP-Rib ADP-riboses ADPribose D-ribofuranose, 5-5'-ester with adenosine 5'-(trihydrogen pyrophosphate) ribose adenosinediphosphate adenosine 5'-[3-(D-ribofuranos-5-O-yl) dihydrogen diphosphate] small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p0dtd1-pro_0000449621 Papain-like protease nsp3 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG 819 2763 adp_d_ribose-rep-5 4 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 adp_d_ribose-rep-1 1 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7 A/B adp_d_ribose-rep-2 1 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7 A/B adp_d_ribose-rep-3 1 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7 A/B adp_d_ribose-r1a_cvhsa-1 1 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7 A/B adp_d_ribose-rep-4 5 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7C All 4 macrodomains tested denatured at higher temperatures with the addition of ADP-ribose. adp_d_ribose-r1a_cvemc-1 5 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7C All 4 macrodomains tested denatured at higher temperatures with the addition of ADP-ribose. adp_d_ribose-macrod2-1 2 8 enzyme enzyme neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 enzyme target enzyme target substrate substrate neutral component neutral component purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) de-adp-ribosylation reaction de-ADP-ribosylation reaction Figure 9 adp_d_ribose-r1a_cvhsa-2 5 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7 C All 4 macrodomains tested denatured at higher temperatures with the addition of ADP-ribose. adp_d_ribose-r1a_cvemc-2 2 9 enzyme target enzyme target substrate substrate neutral component neutral component purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 enzyme enzyme neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) de-adp-ribosylation reaction de-ADP-ribosylation reaction Figure 9 adp_d_ribose-macrod2-2 5 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 7C All 4 macrodomains tested denatured at higher temperatures with the addition of ADP-ribose. parp10-r1a_cvemc-1 2 9 enzyme target enzyme target substrate substrate neutral component neutral component purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) de-adp-ribosylation reaction de-ADP-ribosylation reaction Figure 9 parp10-r1a_cvemc-2 3 9 enzyme target enzyme target substrate substrate neutral component neutral component purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region adprib1res monoadenosine diphosphoribosyl (ADP-ribosyl) modified residue ADP-rybosylation (from NAD) ADPRib1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 enzyme enzyme neutral component neutral component purified purified MAC1_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) de-adp-ribosylation reaction de-ADP-ribosylation reaction Figure 11