IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs. Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs. bioRxiv : the preprint server for biology 2020 imex curation Kern DM., Sorum B., Hoel CM., Sridharan S., Remis JP., Toso DB., Brohawn SG. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus I have not added Mol. Seiving as they state there is a Dimer at ~62kDa - however in Figs S3 and S13 the "dimer" is running at ~30/35 kDa which is the approx. M. wt. of the monomer. I see no bands in either figure of 62 kDa nor tetramer ~120kDa. (monomer Wt is stated to be 31kDa and by calculation is ~33kDa) brohawn@berkeley.edu 28-Sep-2020: Contacted by IntAct-Help. by Birgit Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Kern DM., Sorum B., Hoel CM., Sridharan S., Remis JP., Toso DB., Brohawn SG. Virus - Publications including interactions involving viral proteins I have not added Mol. Seiving as they state there is a Dimer at ~62kDa - however in Figs S3 and S13 the "dimer" is running at ~30/35 kDa which is the approx. M. wt. of the monomer. I see no bands in either figure of 62 kDa nor tetramer ~120kDa. (monomer Wt is stated to be 31kDa and by calculation is ~33kDa) brohawn@berkeley.edu Accepted 2020-AUG-25 AT 12:58 BST AT 12:58 BST by KALPANAP by Birgit bioRxiv : the preprint server for biology 2020 imex curation ap3a_sars2 ORF3a protein Accessory protein 3a Protein U274 Protein X1 3a Protein 3a protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL ap3a_sars2-1 1 2 unspecified role unspecified role neutral component neutral component purified purified post-cleavage tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Table S1