IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Dimerization of Coronavirus nsp9 with Diverse Modes Enhances Its Nucleic Acid Binding Affinity. Dimerization of Coronavirus nsp9 with Diverse Modes Enhances Its Nucleic Acid Binding Affinity. Journal of virology 2018 imex curation Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus ssDNA binding assay were not capture because of no biological significance (data-processing comment added as well) ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins ssDNA binding assay were not capture because of no biological significance (data-processing comment added as well) ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn Accepted 2020-APR-27 AT 21:56 BST AT 21:56 BST by PPORRAS Journal of virology 2018 Only protein-protein interactions imex curation imex curation Dimerization of Coronavirus nsp9 with Diverse Modes Enhances Its Nucleic Acid Binding Affinity. Dimerization of Coronavirus nsp9 with Diverse Modes Enhances Its Nucleic Acid Binding Affinity. Journal of virology 2018 imex curation Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus ssDNA binding assay were not capture because of no biological significance (data-processing comment added as well) ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins ssDNA binding assay were not capture because of no biological significance (data-processing comment added as well) ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn Accepted 2020-APR-27 AT 21:56 BST AT 21:56 BST by PPORRAS Journal of virology 2018 Only protein-protein interactions imex curation imex curation Dimerization of Coronavirus nsp9 with Diverse Modes Enhances Its Nucleic Acid Binding Affinity. Dimerization of Coronavirus nsp9 with Diverse Modes Enhances Its Nucleic Acid Binding Affinity. Journal of virology 2018 imex curation Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus ssDNA binding assay were not capture because of no biological significance (data-processing comment added as well) ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zeng Z., Deng F., Shi K., Ye G., Wang G., Fang L., Xiao S., Fu Z., Peng G. Virus - Publications including interactions involving viral proteins ssDNA binding assay were not capture because of no biological significance (data-processing comment added as well) ssDNA binding assays (EMSA and MST) were not capture as the interaction has no biological significance. penggq@mail.hzau.edu.cn Accepted 2020-APR-27 AT 21:56 BST AT 21:56 BST by PPORRAS Journal of virology 2018 Only protein-protein interactions imex curation imex curation x2g6c4_9nido 3C-like proteinase orf1ab Growth factor-like peptide Helicase NendoU Non-structural protein 10 Non-structural protein 2 Non-structural protein 3 Non-structural protein 4 Non-structural protein 6 Non-structural protein 7 Non-structural protein 8 Non-structural protein 9 ORF1ab polyprotein RNA-directed RNA polymerase Replicase polyprotein 1ab Uridylate-specific endoribonuclease Papain-like proteinase nsp5 p195 protein protein Porcine coronavirus HKU15 Porcine coronavirus HKU15 MAKNKSKRDAIALPENVPPPLQLFIHVAAAEEGHPKVTTYLGNYNLYATKAPPGVQVLSAKTSLTDFENVFGAQPTLRSIRNLVCEARSAEWTISKNAFALKATQLDYSDAVLRAMIRFCPPKVSTLAAFALFGRLVKIEDKELAELARDTALELAYTAKIGTSLADTRSVSLIHKDAYLTLSNEVVGVTFTAALMAKATTVNGAMQYSNFYLYPRATIKVTDGKAEAIATKPLSAATKGKQITEDVNLLPDYQQLLVDQVTGTEVKVGALTYVKTTDSPPLYFPKVKGGVIGIALKQQGTAAKKLNVVFHAQPDDVLLAFIQLQQFLNRTSDSSVEITDCQSYEVSPTVTVKIGPSKPGDVIVATDEEYLKCFETPEVGRLYKVFQTQSWAIIERAFSSLKIRVSKALSAFISFLQNLADNFTAISGVVTALIRELQDLTLDVATRITNIQFVYRAGKLIVDTTSVIAKLFQPFCDFISPFLRKVAGFAIYTVGNRMLMFTSTGTFLLTKATTKILNKAKYIFDVEPEYPVDVTTSKVVVHEALQQTDTKPTRALEAVDVVVGNTVLQMATDGTAFYPSDGTHASLPGFKAGSDELFISFNCDLFDDETNAQINETLAAYELNQLVAPGDSTPRQIATLVVDTLADAITDHFPEKTIDLPEDYQVFSDHDDLPLAQYHIPDHLSLYIQAMEGEDDSGDEICIEDDDYDCPQADEDTEGVIPQQWELPDVDKFLLKIQERKTSSDEVLSVDVYPKPEPVGNVGIDDSASEKKPNGDSVPDPEVHPTLESVDVERPTETANQAVEDKPSDTTFVVDEEQLQESTPEHELRSYEGEFDSDDEIIIPIVPVTPADLKPQTITIKEYFKSEKLETINEGSTESVTQSDDSFDESFVDAESDDPQDPAVYDDTTIITDSTDVGDEPETTLATIVNTPLTLDNNLPPEAIKQPSPTKVELVVGELASIKFDNSVLVNPANAQLTNGGGAARAIAKLAGPKYQEYCNSVAPISGPLTTDSFDAKKFGVACILHVVPPKGSDPNVQELLYQAYKSILTEPAHYVIPILGAGIFGCNPVHSLDAFRKACPSDIGRVTLVTMNKNHLQVWDALNRTIVRTTTDYDQVTTKALTPQGVLEANLFDGEDFVQEPKPGQIYLEVTEEVQNQAKELDLNLQQYCVYLKTCHHKWVVSRTNGLMHLKQKDNNCFVSAGVNLFQNTAYQLRPAIDALYREYLNGNPNRFVAWIYASTNRRVGEMGCPQQVISLLVSNSDAAFSATTACCNTYFNHTGVISVAREYDPIQPKVYCMKCDVWTPFTPQSGKGAVAIGISADEPTGPAIKFAAAHCWYTNGKKTVNGYDTKANVVATYHRFDVPKPQLVEDVVALPTKNDFEVLNVEELPQDSVLHLDPPPVQALQPKANQHIEILENPDYLDILDLWIRKPKFILVKSWSVLGRALCKAGKVVFVSASLLTRFYNYLVEIGALDSTIRLSVDLTCKFVRTVLPSSNTVHKTCLGLYYSAQTLFVSLAPFLMLPAVVSLLNSGYTIGTYLYAKTGWPCNYNATQHFDYNSYCAGDLVCQACFDGQDSLHLYPHLRVNQQPLQTTDYTVYALSLILLLANMTLVMGTLIVTFFVNFYGVQIPFYGTLLIDYQSALVITFSVYYFYKVMKFFRHLTHGCKIPTCVVCAKLRTPPTITVETVVQGRKYPSVIETNGGFTICKEHNFYCKDCSLQTPGTFIPTEAIESLSRATRLSVKPTAPAFLLARDVECQTDVVVARAMHNQNAHVCISKYSDIRTVDQLLKPTPLFSYTPDVIIAADFDNRGSLKTAKELAVVLSMDLKRTIIIIDQAYSRPIDNYQEVASRIEKYYPVAKITPTGDIFTDIKQATNGQASDSAINAAVLAVQRGLDFTIDNPNNILPHYAFDFSTLNAEDQSTILESGCAKGNLKGTNVGVVLSASLVTRLSQQAIRVIANAASRNGVTCAVTPSTLVMRGNIATQPLTRIKAGAPPMRQKILCVILALAIVYFAAMAFGFLASQITLNTVPTIKSDIRASTFYVVRDGVLDTVRSNDKCFANKFLAFDSFIQAPYTNSPDCPVVVGVVDVTTHSIPGIPAGVIHRDGLILNIYEQSLYETHQRQSMVRDALSLKTANLFNLGKRVVVGYTQHEVVVGTSYFNSPALFNAKCTFLQYQDTRQLYCYDTVPTEHKLYSDVLPHVEYKAIDINGDLVPFKIPEQIMFYPHIVRYTSNSYCRMGHCFNTNPGICISFTDEFPYSENVKPGVYCADTSLQLFSNLVLGTVSGIHIFTSTAALLGSTIVIILCVVAVLAVQRFFKEYTTFVMYTCGLALVNIVGIALMYKCLVFAIFYYAIYLYFVLTFPSFKRNVALFYFAVVIVPHVSNMQLLALIVCSIIYFLYTYVHTVAKTAGKFSSFLDAAKATFVIDNEKYVLLKDLAGAEFDQYLASYNKYKYFSGTASDKDYDKVCMAFLAKALSSFREGGGSQLYTPPKFAVVQSLKTKLQAGIKILLHPSGVVERCMVSVVYNGSALNGIWLKNVVYCPRHVIGKFRGDQWTHMVSIADCRDFIVKCPIQGIQLNVQSVKMVGALLQLTVHTNNTATPDYKFERLQPGSSMTIACAYDGIVRHVYHVVLQLNNLIYASFLNGACGSVGYTLKGKTLYLHYMHHIEFNNKTHSGTDLEGNFYGPYVDEEVIQQQTAFQYYTDNVVAQLYAHLLTVDARPKWLAQSQISIEDFNSWAANNSFANFPCEQTNMSYIMGLSQTARVPVERILNTIIQLTTNRDGACIMGSYDFECDWTPEMVYNQAPISLQSGVVKKTCTWFFHFLFMAITMLLAAMHVFPVHLYPIVLPCFTVVAFLLTLTIKHTVVFTTTYLLPSLLMMVVNANTFWIPNTFLRTCYETIFGSPIAQRLYGYTVALYMLIYAGLAINYTLKTLRYRATSFLSFCMQWFQYGYVAHIAYKLLNKPWTESLLFTAFTMLTSHPLLAALSWWLAGRVTLPIIMPDLAIRVLAYNVIGYVICVRFGLMWLANRFTTVPMGTYQYMVSVEQLKYMMAVKMSPPRNAFEVLIANIRLLGLGGNRNIAVSTVQNKILDAKATAVVVANLLEKAGVTNKHAICKKIVKLHNDTLKATTYEEVEVALVKLLSHIIEFLPTDQVDAYLADAANAQHVNTYFDNLLENKAVVQAVADININLDSYRIYKEADAIYKRSVEMNESPQEQKKKLKAVNIAKAEWEREAASQRKLEKLADAAMKSMYLAERAEDRRIKLTSGLTAMLYHMLRRLDSDRVKALFECAKAQILPIHAVVGISNDNLKVIFNDKDSYSHYVEGNTLIHKGVRYTIVKKLSLDNAPIEGVPEEFPVVVETVREGVPQLQNNELCLRNVFTAQNTAQDFNGNESTVKSFYVTRTGKKILVAITSTKDNLKTVTCLTETGKTVLNLDPPMRFAHTVGGKQSVVYLYFIQNISSLNRGMVIGHISETTILQASGTQIEYQQNASLLTYLAFAVDPKTAYLKHLADGGSPIQGCIQMIATMGPGFAVTTKPQPNEHQYSYGGASICLYCRAHIPHPGVDGRCPYKGRFVHIDKDKEPVSFALTHEPCSSCQRWVNYDCTCGSSLQNSAYLNRVTGSSDARLEPLQPGTQPDAVKRAFHVHNDTTSGIFLSTKSNCARFKTTRSALPLPNKGEVELYFVTKQCAAKVFEIEEECYNALSTELYTTDDTFGVLAKTEFFKFDKIPNVNRQYLTKYTLLDLAYALRHLSTSKDVIQEILITMCGTPEDWFGENWFDPIENPSFYKEFHKLGDILNRCVLNANKFASACIDAGLVGILTPDNQDLLGQIYDFGDFIITQPGNGCVDLASYYSYLMPIMSMTHMLKCECMDSDGNPLEYDGFQYDFTDFKLGLFEKYFKYWDRPYHPNTVECPDDRCVLHCANFNVLFAMCIPNTAFGNLCSRATVDGHLVVQTVGVHLKELGIVLNQDVTTHMANINLNTLLRLVGDPTTIASVSDKCVDLRTPCQTLATMSSGIAKQSVKPGHFNQHFYKHLLDSNLLDQLGIDIRHFYYMQDGEAAITDYSYYRYNTPTMVDIKMFLFCLEVADKYLEPYEGGCINAQSVVVSNLDKSAGYPFNKLGKARNYYDMTHAEQNQLFEYTKRNVLPTLTQMNLKYAISAKDRARTVAGVSIISTMTNRQYHQKMLKSISLARNQTIVIGTTKFYGGWDNMLRRLMCNINNPILVGWDYPKCDRSMPNMLRIAASCLLARKHTCCNQSQRFYRLANECCQVLSEVVVSGNNLYVKPGGTSSGDATTAYANSVFNILQVVSANVATFLSTSTTTHLNKDIADLHRSLYEDIYRGDSNDITVINRFYQHLQSYFGLMILSDDGVACIDSAVAKAGAVADLDGFRDILFYQNNVYMADSKCWTETDMNVGPHEFCSQHTVLAEHDGKPYYLPYPDVSRILGACIFVDDVNKADPVQNLERYISLAIDAYPLTKVDPIKGKVFYLLLDYIRVLAQELQDGILDAFQSLTDMSYVNNFMNEAFYAQMYEQSPTLQASGVCVVCNSPTILRCGDCIRRPLLCCVCAYQHVTQTTHKRIIAINNYICSVENCNEDNVEKLFISGTAIYCENHKPTLCIPIVANGSVFGIYRHTARGSDDIDLFNELATSNYDTIEPYQKANRAPLSLMLFAAETIKALEESIKKSYATATVKDVYDQRFIKLLWEQGKKPPPITKNHIFTGYHFNKNGKTQVGDYILAKTDGSDTYTYRGTSTYKLQTGDVLVLMAHVVTPLSAPPVLTQTTYVRKSLLPDSVGASYYVQHFKSYNEIAMQRVTTVLGPPGTGKSTFAIGLAKYFPSARICYTASSHAAIDALCEKAFKTIPVGQCSRIVPTRTTVECFQEFVVNNTTAQYIFSTINALPDIKCDIVVVDEVSMLTNYELSSVNARLVYNHIVYVGDPYQLPSPRTMLTSGQLSPADYNVVTDIMVHAGADVMLDMCYRCPREIVETVSKLVYDNKLKAAKPNSRQCYKTIVNFGPGDVAHEGQSAYNEAQLRFALAFRQQKRWDNVTFISPYNAMNVKASLAGFSTQTVDSSQGSEYDYVIFCVTTDSAHALNMARLNVALTRAKIGILVVFRQANELYNSLQFESIDSQLQSSAEKNLTPLFKRCGYEYNGVHPAHALTWHDCGAEYRCEEPLAKLVGVADGTLISYKTLVSTLGFLPSLKIDAYHNMFLTRDACRTYVQSWIGIDVEAAHAIKPNTGTNLPLQIGFSTGKNFSVTPEGIWVNEHGSCTEPVPAKIPPGEQFRHLKKDMRQARPWKVVRREIATHIAEVAPHTDYICFVTWAHQLELATMRYFVKLGMEEKCFCGRRACFTNGTEFACKAHHSLTIPQCDYVYNPFLIDVATWGFSGRLSTNHDAVCTYHANAHVASADAIMTVCLAIHELFSTVDWNFEFPVTAEQSQLNKACRLVQANYLNILLTTTKATVVHDIGNPKGIPIVRKPGVKYHFYDQAPIVKHVQKLKYKPEMEARFTDGLTMFWNCNVDTYPANALVCRYDTHRQKHLIGPNGSALYVNKHAFLTPEMHTYATHKLNLAPLIYYSTTDCSSEQPIVVTYRDCVTRCNTGKTLCPNHALEYQEFINAYNLMARHGFNVYIPRNVNVYNCWLTFTNLQNLENLAYNCYYKNCNAHVDGQLDVVINNNAVYAKVDNNLVKLFDNRTNLPVSVAFEHYTNRHTRSLPTTQLLSGLGVTATRNFTVWFDNDTIFQYTINVSTYTDIDPSTHVVLCDDRYGTDWSQFNQLPNAVFLTKTKVKKTEPFVCTALTLNGVAIDGEELYIYVRYNNQLTTFATTCTQGRNVEQFIPKTPMERDFLEMSQQSFIQQHQLQELGVEHIIYGDDSSPVIGGTHTLISLVKNKFEHQLVNHVYNPVQNCVVTSPNASSKNVCTVLDVLLDDYIDIIRQAHASYTSKSKVFTVSIDNQQIRFMLWHDEQVKTCYPILQSLTNGYQMPSVYKTLVTDLQPADIPNYHSYTPRVPGVVKNVIKYRQLFNYIVKKDRLAVPHNMTVLHLGAASALGTAPGSSVIKQMFPEGTVLIDLDIREFTSDANQIIVTDYRTYIPPHHVDVIFSDLYCCDDIHFFDNLIRIVKERLALGGSIFVKITEHSFSPELYSLAGWFDDYQLFCTAVNASSSEAFLCCFNYLGLAKENINGFNLHASYIQWRNEIALTPTYSPLADNPATACKLKATPIISARELEKKPILRYLVASGRLLVRPPECRELY AF100075E6666E0A p0c6y4-pro_0000037377 Non-structural protein 9 rep ORF1ab polyprotein 1a-1b protein protein pedv Porcine epidemic diarrhea virus (strain CV777) NNEIIPGKLKQRSIKAEGDGIVGEGKALYNNEGGRTFMYAFISDKPDLRVVKWEFDGGCNTIELEPPRKFLVDSPNGAQIKYLYFVRNLNTLRRGAVLGYIGATVRLQ 3858 3965 8BD2A35DB170C62F nsp9-dimer-1 2 5 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 rep-76 2 4 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range NSP9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.Asn3378_Arg3384del mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain NNELCLR . ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 rep-77 2 5 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0C6Y4-PRO_0000037377:p.Cys59Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Tue Jun 30 19:23:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys59Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 orf1ab-4 1 4 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range NSP9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.Asn3378_Arg3384del delta7N mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain NNELCLR . X2G6C4:p.[Leu3381Ala;Leu3383_Asn3385delinsAlaAlaAla] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain LRN AAA Tue Jun 30 18:59:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu3381Ala;Leu3383_Asn3385delinsAlaAlaAla]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2 H, 2E, 2G orf1ab-5 3 4 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range NSP9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.Asn3378_Arg3384del delta7N mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain NNELCLR . X2G6C4:p.[Leu3381Ala;Leu3383_Asn3385delinsAlaAlaAla] L4A/L6A/R7A/N8A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain LRN AAA Tue Jun 30 16:25:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu3381Ala;Leu3383_Asn3385delinsAlaAlaAla]' X2G6C4:p.Arg3384Ala R7A mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 18:12:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg3384Ala' X2G6C4:p.Gly3473Glu G96E mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 18:22:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly3473Glu' X2G6C4:p.Gly3477Glu G100E mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 18:54:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly3477Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2F rep-78 3 5 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0C6Y4-PRO_0000037377:p.Cys59Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A Tue Jun 30 18:14:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys59Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5E