IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ MERS-CoV 4b protein interferes with the NF-κB-dependent innate immune response during infection. MERS-CoV 4b protein interferes with the NF-κB-dependent innate immune response during infection. PLoS pathogens 2018 imex curation Canton J., Fehr AR., Fernandez-Delgado R., Gutierrez-Alvarez FJ., Sanchez-Aparicio MT., García-Sastre A., Perlman S., Enjuanes L., Sola I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus L.Enjuanes@cnb.csic.es,stanley-perlman@uiowa.edu Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Canton J., Fehr AR., Fernandez-Delgado R., Gutierrez-Alvarez FJ., Sanchez-Aparicio MT., García-Sastre A., Perlman S., Enjuanes L., Sola I. Virus - Publications including interactions involving viral proteins L.Enjuanes@cnb.csic.es,stanley-perlman@uiowa.edu Accepted 2020-JUN-26 AT 16:56 BST AT 16:56 BST by MGT PLoS pathogens 2018 Only protein-protein interactions imex curation imex curation MERS-CoV 4b protein interferes with the NF-κB-dependent innate immune response during infection. MERS-CoV 4b protein interferes with the NF-κB-dependent innate immune response during infection. PLoS pathogens 2018 imex curation Canton J., Fehr AR., Fernandez-Delgado R., Gutierrez-Alvarez FJ., Sanchez-Aparicio MT., García-Sastre A., Perlman S., Enjuanes L., Sola I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus L.Enjuanes@cnb.csic.es,stanley-perlman@uiowa.edu Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Canton J., Fehr AR., Fernandez-Delgado R., Gutierrez-Alvarez FJ., Sanchez-Aparicio MT., García-Sastre A., Perlman S., Enjuanes L., Sola I. Virus - Publications including interactions involving viral proteins L.Enjuanes@cnb.csic.es,stanley-perlman@uiowa.edu Accepted 2020-JUN-26 AT 16:56 BST AT 16:56 BST by MGT PLoS pathogens 2018 Only protein-protein interactions imex curation imex curation MERS-CoV 4b protein interferes with the NF-κB-dependent innate immune response during infection. MERS-CoV 4b protein interferes with the NF-κB-dependent innate immune response during infection. PLoS pathogens 2018 imex curation Canton J., Fehr AR., Fernandez-Delgado R., Gutierrez-Alvarez FJ., Sanchez-Aparicio MT., García-Sastre A., Perlman S., Enjuanes L., Sola I. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus L.Enjuanes@cnb.csic.es,stanley-perlman@uiowa.edu Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Canton J., Fehr AR., Fernandez-Delgado R., Gutierrez-Alvarez FJ., Sanchez-Aparicio MT., García-Sastre A., Perlman S., Enjuanes L., Sola I. Virus - Publications including interactions involving viral proteins L.Enjuanes@cnb.csic.es,stanley-perlman@uiowa.edu Accepted 2020-JUN-26 AT 16:56 BST AT 16:56 BST by MGT PLoS pathogens 2018 Only protein-protein interactions imex curation imex curation ima1_human Importin subunit alpha-1 KPNA2 RCH1 SRP1 Karyopherin subunit alpha-2 SRP1-alpha RAG cohort protein 1 protein protein human Homo sapiens Human MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF DIP protein P52292 original sequence version: 180 DIP protein P52292 original sequence version: 128 B0F94A0475B80EED ima4_human Importin subunit alpha-4 KPNA3 QIP2 Karyopherin subunit alpha-3 SRP1-gamma Importin alpha Q2 protein protein human Homo sapiens Human MAENPSLENHRIKSFKNKGRDVETMRRHRNEVTVELRKNKRDEHLLKKRNVPQEESLEDSDVDADFKAQNVTLEAILQNATSDNPVVQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVKCLERDDNPSLQFEAAWALTNIASGTSAQTQAVVQSNAVPLFLRLLRSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISPSIPITFLRNVTWVIVNLCRNKDPPPPMETVQEILPALCVLIYHTDINILVDTVWALSYLTDGGNEQIQMVIDSGVVPFLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDVLSHFPNLLSHPKEKINKEAVWFLSNITAGNQQQVQAVIDAGLIPMIIHQLAKGDFGTQKEAAWAISNLTISGRKDQVEYLVQQNVIPPFCNLLSVKDSQVVQVVLDGLKNILIMAGDEASTIAEIIEECGGLEKIEVLQQHENEDIYKLAFEIIDQYFSGDDIDEDPCLIPEATQGGTYNFDPTANLQTKEFNF DIP protein O00505 original sequence version: 107 C4FF132C3F346B7F orf4b_mers1 Non-structural protein ORF4b ORF4b protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC MEESLMDVPSTSGTQVYSRKARKRSHSPTKKLRYVKRRFSLLRPEDLSVIVQPTHYVRVTFSDPNMWYLRSGHHLHSVHNWLKPYGGQPVSEYHITLALLNLTDEDLARDFSPIALFLRNVRFELHEFALLRKTLVLNASEIYCANIHRFKPVYRVNTAIPTIKDWLLVQGFSLYHSGLPLHMSISKLHALDDVTRNYIITMPCFRTYPQQMFVTPLAVDVVSIRSSNQGNKQIVHSYPILHHPGF 7529F1F376D53982 ima5_human Importin subunit alpha-5 KPNA1 RCH2 Karyopherin subunit alpha-1 SRP1-beta RAG cohort protein 2 Nucleoprotein interactor 1 protein protein human Homo sapiens Human MTTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEAQISNMEMAPGGVITSDMIEMIFSKSPEQQLSATQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKEACWTISNITAGNRAQIQTVIDANIFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEAKRNGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTEDEDSSIAPQVDLNQQQYIFQQCEAPMEGFQL DIP protein P52294 original sequence version: 72 DIP protein P52294 original sequence version: 90 DIP protein P52294 original sequence version: 112 DIP protein P52294 original sequence version: 160 E8407A3352D6051C tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS DIP protein Q04206 original sequence version: 170 DIP protein Q04206 original sequence version: 173 DIP protein Q04206 original sequence version: 99 DIP protein Q04206-1 original sequence version: 99 DIP protein Q04206 original sequence version: 168 DIP protein Q04206 original sequence version: 128 DIP protein Q04206 original sequence version: 131 DIP protein Q04206 original sequence version: 153 DIP protein Q04206 original sequence version: 185 DIP protein Q04206 original sequence version: 181 DIP protein Q04206 original sequence version: 183 8147A6AF9F2445C6 ima3_human Importin subunit alpha-3 KPNA4 QIP1 Karyopherin subunit alpha-4 Importin alpha Q1 protein protein human Homo sapiens Human MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPHEDICEDSDIDGDYRVQNTSLEAIVQNASSDNQGIQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVHCLERDDNPSLQFEAAWALTNIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISPSIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVNILVDTVWALSYLTDAGNEQIQMVIDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFLSNITAGNQQQVQAVIDANLVPMIIHLLDKGDFGTQKEAAWAISNLTISGRKDQVAYLIQQNVIPPFCNLLTVKDAQVVQVVLDGLSNILKMAEDEAETIGNLIEECGGLEKIEQLQNHENEDIYKLAYEIIDQFFSSDDIDEDPSLVPEAIQGGTFGFNSSANVPTEGFQF DIP protein O00629 original sequence version: 147 DIP protein O00629 original sequence version: 111 D98BC45002C9F57E kpna4-orf4b-1 2 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4a kpna4-orf4b-2 3 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figs. 4b, 5a kpna4-orf4b-3 2 6 unspecified role unspecified role prey prey infection infection K9N643:p.[Arg22_Arg24delinsAlaAlaAla;Lys30_Lys31delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain RKR AAA certain certain sequence position certain certain certain sequence position certain KK AA Tue Jun 30 16:20:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg22_Arg24delinsAlaAlaAla;Lys30_Lys31delinsAlaAla]' K9N643:p.Lys36_Arg38delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KRR AAA Tue Jun 30 19:12:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys36_Arg38delinsAlaAlaAla' 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figs. 4c, 5c, 7 kpna4-orf4b-4 2 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined K9N643:p.[Arg22_Arg24delinsAlaAlaAla;Lys30_Lys31delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain RKR AAA Tue Jun 30 17:49:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg22_Arg24delinsAlaAlaAla;Lys30_Lys31delinsAlaAla]' K9N643:p.Lys36_Arg38delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KRR AAA Tue Jun 30 19:57:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys36_Arg38delinsAlaAlaAla' 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 4d kpna3-orf4b-1 3 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5a kpna2-orf4b-1 3 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5a-b Weak interaction was observed kpna2-orf4b-2 3 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5a-b Weak interaction was observed kpna1-orf4b-1 2 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey infection infection physical association physical association Fig. 5c Very weak interaction was observed. kpna4-orf4b-5 2 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey infection infection K9N643:p.[Arg22_Arg24delinsAlaAlaAla;Lys30_Lys31delinsAlaAla] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain RKR AAA Tue Jun 30 18:06:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg22_Arg24delinsAlaAlaAla;Lys30_Lys31delinsAlaAla]' K9N643:p.Lys36_Arg38delinsAlaAlaAla mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain KRR AAA Tue Jun 30 16:56:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys36_Arg38delinsAlaAlaAla' binding region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 7 Immunoprecipitation of KPNA4 did not pull-down p65 while it did it in delta-4b and 4b NLS mutant virus infected cells. orf4b-kpna3-1 1 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. S5