HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain. Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain. Virology journal 2017 imex curation Wang Q., Li Y., Dong H., Wang L., Peng J., An T., Yang X., Tian Z., Cai X. Virus - Publications including interactions involving viral proteins aci139@sina.com,tzj@hvri.ac.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang Q., Li Y., Dong H., Wang L., Peng J., An T., Yang X., Tian Z., Cai X. Virus - Publications including interactions involving viral proteins aci139@sina.com,tzj@hvri.ac.cn pjm7614@163.com,li315yanwei@126.com Accepted 2019-APR-23 AT 13:31 BST AT 13:31 BST by PPORRAS Virology journal 2017 Only protein-protein interactions imex curation imex curation Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain. Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain. Virology journal 2017 imex curation Wang Q., Li Y., Dong H., Wang L., Peng J., An T., Yang X., Tian Z., Cai X. Virus - Publications including interactions involving viral proteins aci139@sina.com,tzj@hvri.ac.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Wang Q., Li Y., Dong H., Wang L., Peng J., An T., Yang X., Tian Z., Cai X. Virus - Publications including interactions involving viral proteins aci139@sina.com,tzj@hvri.ac.cn pjm7614@163.com,li315yanwei@126.com Accepted 2019-APR-23 AT 13:31 BST AT 13:31 BST by PPORRAS Virology journal 2017 Only protein-protein interactions imex curation imex curation Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain. Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain. Virology journal 2017 imex curation Wang Q., Li Y., Dong H., Wang L., Peng J., An T., Yang X., Tian Z., Cai X. Virus - Publications including interactions involving viral proteins aci139@sina.com,tzj@hvri.ac.cn 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-marc145 chlae MARC- 145 cells Green monkey Cercopithecus aethiops marc145 African green monkey kidney epithelial cell line MARC- 145 cells Parent cell line is MA-104. The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators. Whitaker & Hayward (1985) found the cell line to originate from an African Green monkey and not a Rhesus macaque as was originally claimed. PMID: 4043530 anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Wang Q., Li Y., Dong H., Wang L., Peng J., An T., Yang X., Tian Z., Cai X. Virus - Publications including interactions involving viral proteins Only the 5 chlae proteins from table S1 are entered. There are also 215 interacting partners found but mapped to other primate species including: A9L913, F6QGV0, F6QMV0, F6QN95, F6QWE2, F6R1E4, F6R4J1, F6R5W0, F6RFU8, F6RWK6, F6S443, F6S9R5, F6SQ40, F6TII3, F6TM63, F6TUP0, F6TVL9, F6U0Z8, F6U8N3, F6UR30, F6VER2, F6VFF5, F6VM61, F6WAB3, F6WE02, F6WHT9, F6WJJ6, F6WKH0, F6WU88, F6WZW8, F6X9G9, F6XAZ4, F6XL94, F6XLA4, F6XTF7, F6XUZ6, F6XZM8, F6Y3Q6, F6Y9M4, F6YQX3, F6Z1X9, F6Z5M1, F6Z8Y2, F6ZBZ9, F7A670, F7A782, F7ALC4, F7ATJ0, F7ATW4, F7AW94, F7BA07, F7BGE6, F7BHA4, F7BRW5, F7BX23, F7C2X2, F7CA88, F7CGS1, F7CHC3, F7CL39, F7D5P2, F7DBC0, F7DF58, F7DHQ7, F7DHR2, F7DJM1, F7DP06, F7DZD6, F7E4F9, F7EBT6, F7EDH4, F7ESV5, F7ETD0, F7EV45, F7F3I6, F7FLE3, F7FQQ4, F7FWQ8, F7FZ84, F7G108, F7G7F8, F7GNB2, F7GRH3, F7GUA1, F7GV47, F7GW57, F7GYJ9, F7GZY5, F7H0W4, F7H259, F7H2B1, F7H2G1, F7H3Z1, F7HAC6, F7HAN9, F7HC59, F7HD92, F7HE86, F7HGD2, F7HII5, F7HIX4, F7HJM2, F7HK11, F7HLX2, F7HNZ0, F7HQ35, F7HR57, F7HS29, F7HSB5, G7MJ54, G7N0D2, G7N138, G7N265, G7N293, G7N3P9, G7N3Q0, G7N7V5, G7N9K6, G7NAH3, G7NE24, G7NER4, G7NGF5, G7NHG5, G7NII9, G7NKT8, G7NP88, G7NPT2, G7NQT9, G7P6J9, G7P7J9, H9EM10, H9EMZ8, H9ENW2, H9EP12, H9EP23, H9EPM2, H9EQ62, H9ERH9, H9ES13, H9ESD6, H9ESN2, H9ETI5, H9ETT3, H9EUI5, H9EUT7, H9FPI0, H9FT57, H9FUX2, H9FVY9, H9FWM8, H9FYW7, H9FZW0, H9G0J7, H9G1E0, H9YV34, H9YWU5, H9YX92, H9Z621, H9Z7W5, H9ZB05, I0FGZ2, I0FIG4, I0FQ09, I0FVF2, I2CUF3, I2CYV6, I7G5Z1, J9QHW1, P61258, P61270, P61271, P69895, Q25PV7, Q25Q65, Q28521, Q28758, Q2PFX4, Q4R362, Q4R3D4, Q4R4J7, Q4R4K6, Q4R4P1, Q4R4T5, Q4R4X8, Q4R4Z6, Q4R502, Q4R506, Q4R531, Q4R538, Q4R559, Q4R561, Q4R5B0, Q4R5B2, Q4R5B6, Q4R5C2, Q4R5G7, Q4R5H8, Q4R5M0, Q4R5P2, Q4R5Q0, Q4R6J9, Q4R6P8, Q4R723, Q4R7Y2, Q4R7Y4, Q4R8Z4, Q5IFJ7, Q60HC2, Q76N69, Q8HXB8, Q8HXN9, Q8HXX4, Q8HXY0, Q8HXY8, Q9BE24. aci139@sina.com,tzj@hvri.ac.cn pjm7614@163.com,li315yanwei@126.com Accepted 2019-APR-23 AT 13:31 BST AT 13:31 BST by PPORRAS Virology journal 2017 Only protein-protein interactions imex curation imex curation k7gs49_pig ILF2 protein protein pig Sus scrofa (Pig) Pig MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPAEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEETPQGEEEESMETQE 3A452222DCD1FFA0 actb_chlae Actin, cytoplasmic 1 ACTB Beta-actin protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF mint 6AFD05CA94E360E2 a0a287abg2_pig Proliferating cell nuclear antigen PCNA protein protein pig Sus scrofa (Pig) Pig MPRRCDSTTMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSPTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS 074E3AE23AFB3E82 rs4x_chlae 40S ribosomal protein S4, X isoform RPS4X protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG 87200E545A8958B0 ucri_chlae Cytochrome b-c1 complex subunit Rieske, mitochondrial UQCRFS1 Complex III subunit 5 Cytochrome b-c1 complex subunit 5 Rieske iron-sulfur protein Rieske protein UQCRFS1 Ubiquinol-cytochrome c reductase iron-sulfur subunit protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEXPVLDLKRPXLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDVKVPDFYDYRRLEVLDSTKSSRESSEARKGFSYLVTAVTTVGVAYAAKNVVTQFISSMSASADVLAMAKIEINLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEEEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPPYEFTGDDVVVVG 7932FAE598CC60B0 cox2_chlae Cytochrome c oxidase subunit 2 MT-CO2 COII COXII MTCO2 Cytochrome c oxidase polypeptide II COX2 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MAHPVQLGLQDATSPVMEELITFHDYALMTISLISFLVLYALFSTLTTKLTNTNITDAQEMETTWTILPAVILILIALPSLRILYLTDEINNPSFTIKSIGHQWYWTYEYTDYGGLIFNSYMLPPLFLNPGDLRLLEVDNRVVLPIEAPVRMMITSQDVLHSWTIPTLGLKTDAVPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVAELIPLKIFEMGPVFTL 4ABA48D4E5B22120 h9bfk2_chlae Transferrin receptor protein 1 Tfrc protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLGVDEEENADNNTKANGTKPKRCGGNICYGTIAVIIFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPEEDFPAAPRLYWDDLKKKLSEKLDATDFTSTIKLLNENLYVPREAGSQKDENLALYIENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGGLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLDSPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDRTKFPIVKADLSFFGHAHLGTGDPYTPGFPSFNHTQFPQSQSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCKMVTSENKSVKLTVSNVLKETKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSSVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQDVKHPVTGRSLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTSMDTYKELVERIPELNKVARAAAEVAGQFVIKLTHDIELNLDYERYNSQLLLFLRDLNQYRADVKEMGLSLQWLYSARGDFFRATSRLTTDFRNAEKTDKFVMKKLNDRVMRVEYYFLSPYVSPKESPFRHVFWGSGAHTLSALLENLKLRRQNNSAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF 5949136EBA530A3A a0fjb3_prrsv ORF6 GP6 M protein protein prrsv Porcine reproductive and respiratory syndrome virus MGSSLDDFCNDSTAPQKVLLAFSITYTPVMIYALKVSRGRLLGLLHLLIFLNCAFTFGYMTFVHFESTNRVALTMGAVVALLWGVYSAIETWKFITSRCRLCLLGRKYILAPAHHVESAAGFHPIAANDNHAFVVRRPGSTTVNGTLVPGLKSLVLGGRKAVKQGVVNLVKYAK 76CBA6EA1D2F0A0E m-nf45-1 1 4 unspecified role unspecified role prey prey n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3A m-nf45-2 1 4 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3B m-pcna-1 1 6 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3B m-pcna-2 1 6 unspecified role unspecified role prey prey n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3A m-nf45-3 2 4 unspecified role unspecified role neutral component neutral component n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 4 pcna-m-1 2 6 unspecified role unspecified role neutral component neutral component n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component n terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 4 orf6-actb-1 3 5 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association 1 and table S1