HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Journal of virology 2016 imex curation Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-NOV-11 AT 13:41 GMT AT 13:41 GMT by MGT Journal of virology 2016 imex curation Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Journal of virology 2016 imex curation Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-NOV-11 AT 13:41 GMT AT 13:41 GMT by MGT Journal of virology 2016 imex curation Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Journal of virology 2016 imex curation Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-NOV-11 AT 13:41 GMT AT 13:41 GMT by MGT Journal of virology 2016 imex curation Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Journal of virology 2016 imex curation Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-pk15 pig pk15 Pig pig-pk15 pig kidney pk15 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-NOV-11 AT 13:41 GMT AT 13:41 GMT by MGT Journal of virology 2016 imex curation Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity. Journal of virology 2016 imex curation Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li LF., Yu J., Li Y., Wang J., Li S., Zhang L., Xia SL., Yang Q., Wang X., Yu S., Luo Y., Sun Y., Zhu Y., Munir M., Qiu HJ. Virus - Publications including interactions involving viral proteins huajiqiu@hvri.ac.cn Accepted 2016-NOV-11 AT 13:41 GMT AT 13:41 GMT by MGT Journal of virology 2016 imex curation b2xws1_pig GBP1 protein protein pig Sus scrofa (Pig) Pig MASKVHMPEPQCLIENINGRLVVNPKALKLLSAIKQPLVVVAIVGLYRTGKSYLMNKLAGKNKGFSLGSTVQSHTKGIWMWCVPHPKKREHTLALLDTEGLGDVEKGDNQNDSWIFALAILLSSTFVYNSMGTINQQAMDQLHYVTELTERIRAKSEPDEGIEDSADFVSFFPDFVWTLRDFSLDLETDGESITADEYLENSLKLKKGTSQKDKNFNLPRLCIRKFFPKKKCFIFDRPTQRKKLGQLEDLGDDELDSEFVQQAAEFCSYIFLNSKTKTLSGGIKVTGPRLETLVLTFVNTISSGDLPCVENAVLALAEIENSAAVQKAIAHYEQQMAEKLQLPTETLQELLDLHRDVEKEAIDIFMKNSFKDEENVFQKELATKLDKKRDEFCDQNVKASSERCSALIKEIFSPLEEGVKQGLYSKSGGYRLYVEKKEELKIKYLETPRKGLQAEEILQEYLKSKESVGEAILQTDQTLSEKEKQIEVERVRAEAAQAAAKMLEEMQIRNQQIMEQKEKSYQEHVRQLAEKMEKDRAQMLEEQERTMALKLQEQARLIQEGFQEENRRLHNEIQNLQKKMKKSKKECFLS 1A34DD743C3D4AFA q5u8x5_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus Classical swine fever virus MELNHFELLYKTNKQKPMGVEEPVYDVTGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLNNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFNEAQFCEVTKRIGRVTGSDGKLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVITIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADINVVTQARNRPTTLTGCKKGKNFSFAGTIIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLSTAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSYEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGRLACKEDYRYALSSTNEIGLLGAGGLTTTWEEYSHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKGALLTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRREKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGQVKQCKWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISAEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYVLWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVAKGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVITVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWKGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSSKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIRNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALKDSKEDIMKYGLWGTHTALYKSISARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYKVTKYYDDNLSEIKPVIKMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYNGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIRPAFGEKVTPEMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKDITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNDWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTAGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMTEVPVICADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV 221D3B27E69E8E44 ns5a-gbp1-1 1 6 unspecified role unspecified role bait bait B2XWS1:p.Arg48Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R P Wed May 02 14:31:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg48pro' Tue Jun 30 20:05:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg48Pro' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B2XWS1:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:14:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys51ala' Tue Jun 30 15:46:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Ala' 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS5A region binding region binding-associated region binding region range range range range 11 15 physical association physical association 6A, 6E, 7C, 7D, 8C, 8D gbp1-q5u8x5_9flav-1 1 6 unspecified role unspecified role prey prey B2XWS1:p.Arg48Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R P Wed May 02 19:14:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg48pro' Tue Jun 30 19:52:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg48Pro' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B2XWS1:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:35:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys51ala' Tue Jun 30 17:30:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Ala' 7 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS5A region binding region binding-associated region binding region range range range range physical association physical association 7E q5u8x5_9flav-gbp1-1 1 6 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS5A region binding region binding-associated region binding region range range range range physical association physical association 6B q5u8x5_9flav-gbp1-3 5 6 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS5A region binding region binding-associated region binding region range range range range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 6C gbp1-1 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey NS5A region binding region binding-associated region binding region range range range range physical association physical association 6D gbp1-q5u8x5_9flav-2 2 6 unspecified role unspecified role neutral component neutral component flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B2XWS1:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:06:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys51ala' Tue Jun 30 17:53:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Ala' 7 unspecified role unspecified role neutral component neutral component NS5A region binding region binding-associated region binding region range range range range myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 6F, 7F gbp1-q5u8x5_9flav-3 4 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component NS5A region binding region binding-associated region binding region range range range range colocalization colocalization 6G