HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling 2016 imex curation Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca sylvia.vandenhurk@usask.ca. Accepted 2016-AUG-10 AT 10:16 BST AT 10:16 BST by MGT 2016 imex curation VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling 2016 imex curation Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation bovin-mdbk Bos taurus mdbk Madin-Darby bovine kidney cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins Cells infected with BoHV-1 sylvia.vandenhurk@usask.ca sylvia.vandenhurk@usask.ca. Accepted 2016-AUG-10 AT 10:16 BST AT 10:16 BST by MGT 2016 imex curation VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling 2016 imex curation Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca sylvia.vandenhurk@usask.ca. Accepted 2016-AUG-10 AT 10:16 BST AT 10:16 BST by MGT 2016 imex curation VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling 2016 imex curation Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed confocal microscopy confocal microscopy immunostaining immunostaining Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca sylvia.vandenhurk@usask.ca. Accepted 2016-AUG-10 AT 10:16 BST AT 10:16 BST by MGT 2016 imex curation VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling 2016 imex curation Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation bovin-mdbk Bos taurus mdbk Madin-Darby bovine kidney cells confocal microscopy confocal microscopy immunostaining immunostaining Sharmin Afroz, Robert Brownlie, Michel Fodje, Sylvia van Drunen Littel-van den Hurk Virus - Publications including interactions involving viral proteins Cells infected with BoHV-1/BoHV-1-UL47R. sylvia.vandenhurk@usask.ca sylvia.vandenhurk@usask.ca. Accepted 2016-AUG-10 AT 10:16 BST AT 10:16 BST by MGT 2016 imex curation stat1_human Signal transducer and activator of transcription 1-alpha/beta STAT1 Transcription factor ISGF-3 components p91/p84 protein protein human Homo sapiens Human MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNTV 054A813522364BA6 q4r4g2_chlae Signal transducer and activator of transcription STAT1 alpha protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYNCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLNPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPYFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKFLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNAV D351514CFC8692C3 b0jyl6_bovin Signal transducer and activator of transcription STAT1 protein protein bovin Bos taurus (Bovine) Bovine MSQWYELQQLESKYLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIICNCLKEERKILDHAQRISQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMSIEHEIKTLEDLQDEYDFKCKTLQNREHETNGVAKNDQKQEQLLLQKMYLMLDNKRKEVVLKIIELLNATELTQKALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQALWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGARTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLNPPCARWSQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNAGPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLALWNDGCIVGFISKERERALLKDQQPGTFLLRFSESCREGAITFTWVERSQNGGEPYFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKFLYPNIDKDHAFGKYYSRPKEEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRMVGSVEFDMMNAV 63A51E9411A876AB q4vw77_9alph UL47 AAADCAAH_00010 BLEONNCJ_00010 BLPDLEPH_00010 DCJDKEDG_00010 DILPLKIK_00010 DJCKHMNK_00010 NBBNDGNH_00010 NCKHNGOI_00010 NFOBEAPH_00010 OCMKPLLD_00010 OHMFJBFK_00010 protein protein Bovine herpesvirus 1 Bovine herpesvirus 1 MDAARDGRPERRPRRSGTYRTHPFQRPSARRSLLDALRAADAEAAERPRVRRPRPDFQRPPDEDTSEDENVYDYIDGDSSDSADDYDSDYFTANRGPNHGAGDAMDTDAPPERAPEGGAPQDYLTAHLRAIEALPESAPHRSLLERTARTVYAQQFPPRDLSAGSRAPAQRARRSLRGFPRGGGGGQEPGPDDEGDDAADLREDLVPDEAYAHLERDERLSEGPPLLNMEAAAAAAGERSVVEELFTYAPAQPQVEVPLPRILEGRVRPSAFFAQMPLDALCRTPPNDQRVVRERRAWEMAGTPHGLLITTWSTVDPEFSIGGMYVGAPEGTRPRLVWRRAMKQAMALQYRLGVGGLCRAVDGAAMPPTEALLFLADALLRVVRNCHFLSRPGRAGGAARRLPAAAVGLLAATQFTPPDASPHATLFRGSMGSLIYWHELRVMLTAVPALCARYAGAGLQSAELYLLALAHSEAPGYTANERYALSAYLTLFVALAERALRWLYLAGAHLLGPHPTAAAFREVRAKIPYERLPLGSATLHDAEVETVDSATFQEALAFSALAHVYGEAYVAVRTATTLLMAEYAAHAERRDVREMTAAFLGVGLIAQRLMGSLNLLLNCVAGAAVYGGRRVTVREGTLARYSLLADAALPLVRPVSLVEFWEARDGVMRELRLRPVASPPLAGKRRVMELYLSLDSIEALVGREPLGSRPVLGPLVDIAEALADHPHLVTGDGRGPRLGGR 3A04260F20E877EC vp8-stat1-1 1 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2A ul47-stat1-1 3 6 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2B and 4 stat1-ul47-1 2 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 2D stat1-ul47-2 2 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 2E stat1-ul47-3 5 8 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization 9 and 10 In the absence of VP8, Ifn-beta treatment resulted in nuclear localisation of STAT1. stat1 alpha-ul47-1 4 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization 8 In almost all cells that expressed full length VP8, or VP8 minus the NLS, STAT1 remained cytoplasmic, before or after IFN-beta treatment.