HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication. Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication. Journal of virology 2014 imex curation Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins JCForrest@uams.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins JCForrest@uams.edu Accepted 2016-MAY-18 AT 14:26 BST AT 14:26 BST by MGT Journal of virology 2014 imex curation Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication. Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication. Journal of virology 2014 imex curation Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins JCForrest@uams.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins JCForrest@uams.edu Accepted 2016-MAY-18 AT 14:26 BST AT 14:26 BST by MGT Journal of virology 2014 imex curation Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication. Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication. Journal of virology 2014 imex curation Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins JCForrest@uams.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC., Salinas E., Byrum SD., Moreland LE., Mackintosh SG., Tackett AJ., Forrest JC. Virus - Publications including interactions involving viral proteins Mass spectrometry and SILAC-based interaction screen, here called I-DIRT (isotopic differentiation of interactions as random or targeted). Proteins with a light isotopic fraction greater than one standard deviation from a mean contaminant threshold (score of >0.7528 L/L+H) are scored as specific interactions and are included here. JCForrest@uams.edu Accepted 2016-MAY-18 AT 14:26 BST AT 14:26 BST by MGT Journal of virology 2014 imex curation o41925_mhv68 GAMMAHV.M3 M3 protein protein mhv68 Murid herpesvirus 4 (MuHV-4) Murine gammaherpesvirus 68 MAFLSTSVLIKCCILLLAGGLAESLTLGLAPALSTHSSGVSTQSVDLSQIKRGDEIQAHCLTPAETEVTECAGILKDVLSKNLHELQGLCNVKNKMGVPWVSVEELGQEIITGRLPFPSVGGTPVNDLVRVLVVAESNTPEETPEEEFYAYVELQTELYTFGLSDDNVVFTSDYMTVWMIDIPKSYVDVGMLTRATFLEQWPGAKVTVMIPYSSTFTWCGELGAISEESAPQPSLSARSPVCKNSARYSTSKFCEVDGCTAETGMEKMSLLTPFGGPPQQAKMNTCPCYYKYSVSPLPAMDHLILADLAGLDSLTSPVYVMAAYFDSTHENPVRPSSKLYHCALQMTSHDGVWTSTSSEQCPIRLVEGQSQNVLQVRVAPTSMPNLVGVSLMLEGQQYRLEYFGDH 7C8B26A064E20DB2 hsp7c_mouse Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 Hspa8 Hsc70 Hsc73 protein protein mouse Mus musculus Mouse MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD DIP protein P63017 original sequence version: 76 DIP protein P63017 original sequence version: 118 03A27B30E6C076ED rl31_mouse 60S ribosomal protein L31 Rpl31 protein protein mouse Mus musculus Mouse MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN BA9DBE79B9E1C071 ro52_mouse E3 ubiquitin-protein ligase TRIM21 Trim21 Ro52 Ssa1 52 kDa Ro protein 52 kDa ribonucleoprotein autoantigen Ro/SS-A Ro(SS-A) Sjoegren syndrome type A antigen Tripartite motif-containing protein 21 RING-type E3 ubiquitin transferase TRIM21 protein protein mouse Mus musculus Mouse MSPSTTSKMSLEKMWEEVTCSICLDPMVEPMSIECGHCFCKECIFEVGKNGGSSCPECRQQFLLRNLRPNRHIANMVENLKQIAQNTKKSTQETHCMKHGEKLHLFCEEDGQALCWVCAQSGKHRDHTRVPIEEAAKVYQEKIHVVLEKLRKGKELAEKMEMDLTMQRTDWKRNIDTQKSRIHAEFALQNSLLAQEEQRQLQRLEKDQREYLRLLGKKEAELAEKNQALQELISELERRIRGSELELLQEVRIILERSGSWNLDTLDIDAPDLTSTCPVPGRKKMLRTCWVHITLDRNTANSWLIISKDRRQVRMGDTHQNVSDNKERFSNYPMVLGAQRFSSGKMYWEVDVTQKEAWDLGVCRDSVQRKGQFSLSPENGFWTIWLWQDSYEAGTSPQTTLHIQVPPCQIGIFVDYEAGVVSFYNITDHGSLIYTFSECVFAGPLRPFFNVGFNYSGGNAAPLKLCPLKM 393AE5AFD254855B tra2b_mouse Transformer-2 protein homolog beta Silg41 Transformer-2 protein homolog B Splicing factor, arginine/serine-rich 10 Silica-induced gene 41 protein Tra2b Sfrs10 protein protein mouse Mus musculus Mouse MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRGGYRSRSRSRSYSPRRY mint Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing 60B310C8BA443E28 o41974_mhv68 GAMMAHV.ORF73 73 protein protein mhv68 Murid herpesvirus 4 (MuHV-4) Murine gammaherpesvirus 68 MPTSPPTTRNTTSGKTRSGCKRRCFNKPAAMPPKRRRAPKRPAPPPPPGCQGDEESSQGTQTPNPPSPPVPPSSPTLPSSPVPPSSPVHEPPSPSPPPAPPSPDVDVEGLDVGETDDPGPPPPKRYSRYQKPHNPSDPLPKKYQGMRRHLQVTAPRLFDPEGHPPTHFKSAVMFSSTHPYTLNKLHKCIQSKHVLSTPVSCLPLVPGTTQQCVTYYLLSFVEDKKQAKKLKRVVLAYCEKYHSSVEGTIVKAKPYFPLPEPPTEPPTDPEQPSTSTQASGTQHGPTASLDAGAEQGATGSPGSSPGQQGQGSQT mint CBD89BDDF8776DC7 srsf7_mouse Serine/arginine-rich splicing factor 7 Srsf7 Sfrs7 Splicing factor, arginine/serine-rich 7 protein protein mouse Mus musculus Mouse MRSSARGRPLQAATAFFLSLFFFLRRFERGFWLWGGDSETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPRRSRFDRPPARRPFDPNDRCYECGEKGHYAYDCHRYSRRRRSRSRSRSHSRSRGRRYSRSRSRSRGRRSRSASPRRSRSVSLRRSRSASLRRSRSGSIIGSRYFQSRSRSRSRSRSISRPRSSRSKSRSPSPKRSRSPSGSPHRSASPERMD DIP protein Q8BL97 original sequence version: 69 F193FC4C3A7D8C7C ef1a1_mouse Elongation factor 1-alpha 1 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Eef1a1 Eef1a protein protein mouse Mus musculus Mouse MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK DIP protein P10126 original sequence version: 149 71072871DE7405DC h12_mouse Histone H1.2 H1-2 H1 VAR.1 H1c H1f2 Hist1h1c protein protein mouse Mus musculus Mouse MSEAAPAAPAAAPPAEKAPAKKKAAKKPAGVRRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGILVQTKGTGASGSFKLNKKAASGEAKPQAKKAGAAKAKKPAGAAKKPKKATGAATPKKAAKKTPKKAKKPAAAAVTKKVAKSPKKAKVTKPKKVKSASKAVKPKAAKPKVAKAKKVAAKKK DIP protein P15864 original sequence version: 107 2872A9BCD50C840D rl17_mouse 60S ribosomal protein L17 Rpl17 protein protein mouse Mus musculus Mouse MVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLKKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKISQKKLKKQKLMPRE 3337867F610D1CA0 d0u1p1_9gama ORF52 protein protein Wood mouse herpesvirus Wood mouse herpesvirus MASKKPDKTYEEMVKEVERLKLENKSLKQKVKSSGAVPSDDSILTAAKRESIIVSSSRALGAVASRKIEAKVRSRAAKAVTEQELTTMLQSLTLRVDVSMEETTMGATGGIGPSSNTETKKRKPRSKSRHRHDHE 8C463104A19B3B35 rl21_mouse 60S ribosomal protein L21 Rpl21 protein protein mouse Mus musculus Mouse MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIIVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRHAAPPREAHFVRTNGKEPELLEPIPYEFMA mint 74F215B19EA63CAB d0u1p6_9gama ORF57 protein protein Wood mouse herpesvirus Wood mouse herpesvirus MAQQMLEAGALDHMMEGLPSDFDFDTSDEEGELSDSPPAEESTGPVRDVVYEPDPMFDDPPPTPSPDIRSPSPKARKRALSPEIIHNSPLLRDTTKYEPATKRSYSYHPRRSPQRENPRQKLKRGTDSRRPNRWNQKNPKQYWSLKPLLDYSKIPRAEYKNAKLLVPTTGKLRPEFYTDRFVDAIIQNAARNCPVSEKAVSLKNIEESFKLLHSFFNSGINKEPWLSTRYFAIFNNGLVVLTHMLDEQMAWAYACLKHGRELPTDDILMSTSEKLSQQLVIKLMEVIKCIEKDGIFSRILKGVADAVCLKAQFLRGMITLKRTPCSLPMYTLFVYVLTIPTLRTRVIRDPLLTQCKDVVLKYQPGDCITLLKAALNCHQCNKDCDKCKYILDPLLGQTHRTKGVFFVCE D12DA877A32ECE52 srsf6_mouse Serine/arginine-rich splicing factor 6 Srsf6 Sfrs6 Srp55 Pre-mRNA-splicing factor SRP55 Splicing factor, arginine/serine-rich 6 protein protein mouse Mus musculus Mouse MPRVYIGRLSYNVREKDIQRFFSGYGRLLEIDLKNGYGFVEFEDSRDADDAVYELNSKELCGERVIVEHARGPRRDRDGYSYGSRSGGGGYSSRRTSGRDKYGPPVRTEYRLIVENLSSRCSWQDLKDFMRQAGEVTYADAHKERTNEGVIEFRSYSDMKRALDKLDGTEINGRNIRLIEDKPRTSHRRSYSGSRSRSRSRRRSRSRSRRSSRSRSRSISKSRSRSRSRSKGRSRSRSKGRKSRSKSKSKPKSDRGSHSHSRSRSKDKYGKSRSRSRSRSPKENGKGDIKSKSRSRSQSRSHSPLPAPPSKARSMSPPPKRASRSRSRSRSRSRSSSRD AE8B6650D7294A0D actb_mouse Actin, cytoplasmic 1 Actb Beta-actin protein protein mouse Mus musculus Mouse MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 6AFD05CA94E360E2 lana-multi-1 3 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 16 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Figure 1B and S3 lana-orf52-1 2 9 unspecified role unspecified role bait bait c terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 14 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2 mlana-mlana-1 2 9 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait c terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2 mlana_hsc70-1 2 9 unspecified role unspecified role bait bait c terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3B mlana-hspa8-1 1 9 unspecified role unspecified role bait bait purified purified c terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 3A mlana-mlana-2 1 9 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role bait bait purified purified c terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association 2 and 3A