HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology 2015 imex curation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg Accepted 2015-NOV-26 AT 08:51 GMT AT 08:51 GMT by ORCHARD Virology 2015 imex curation Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology 2015 imex curation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg Accepted 2015-NOV-26 AT 08:51 GMT AT 08:51 GMT by ORCHARD Virology 2015 imex curation Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology 2015 imex curation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg Accepted 2015-NOV-26 AT 08:51 GMT AT 08:51 GMT by ORCHARD Virology 2015 imex curation Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology 2015 imex curation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg Accepted 2015-NOV-26 AT 08:51 GMT AT 08:51 GMT by ORCHARD Virology 2015 imex curation Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology 2015 imex curation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins An interaction between SH and P450 cytochrome oxido-reductase was observed but could not be mapped to a single gene product. jtorres@ntu.edu.sg Accepted 2015-NOV-26 AT 08:51 GMT AT 08:51 GMT by ORCHARD Human adult lung Virology 2015 imex curation Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology 2015 imex curation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-c2c12 Mouse C3H muscle myoblast c2c12 Mouse C3H muscle myoblast fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Li Y., Jain N., Limpanawat S., To J., Quistgaard EM., Nordlund P., Thanabalu T., Torres J. Virus - Publications including interactions involving viral proteins jtorres@ntu.edu.sg Accepted 2015-NOV-26 AT 08:51 GMT AT 08:51 GMT by ORCHARD Virology 2015 imex curation rl7_human 60S ribosomal protein L7 RPL7 Large ribosomal subunit protein uL30 protein protein human Homo sapiens Human MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN 070EB4C904E6795A bap31_human B-cell receptor-associated protein 31 BCAP31 BAP31 p28 6C6-AG tumor-associated antigen DXS1357E Protein CDM protein protein human Homo sapiens Human MSLQWTAVATFLYAEVFVVLLLCIPFISPKRWQKIFKSRLVELLVSYGNTFFVVLIVILVLLVIDAVREIRKYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQKLKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGPMDKKEE D34367F870D6EB00 q77yb1_hrsva Small hydrophobic protein SH (1A) Small protein 1A protein protein hrsva human respiratory sy MENTSITIEFSSKFWPYFTLIHMITTIISLLIIISIMIAILNKLCEYNVFHNKTFELPRARVNT rl3_human 60S ribosomal protein L3 RPL3 HIV-1 TAR RNA-binding protein B Large ribosomal subunit protein uL3 OK/SW-cl.32 protein protein human Homo sapiens Human MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA A607CEE75CF62148 rs13_human 40S ribosomal protein S13 RPS13 Small ribosomal subunit protein uS15 protein protein human Homo sapiens Human MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA 23F94D38F87B8D53 taldo_human Transaldolase TALDO1 TAL TALDO TALDOR protein protein human Homo sapiens Human MSSSPVKRQRMESALDQLKQFTTVVADTGDFHAIDEYKPQDATTNPSLILAAAQMPAYQELVEEAIAYGRKLGGSQEDQIKNAIDKLFVLFGAEILKKIPGRVSTEVDARLSFDKDAMVARARRLIELYKEAGISKDRILIKLSSTWEGIQAGKELEEQHGIHCNMTLLFSFAQAVACAEAGVTLISPFVGRILDWHVANTDKKSYEPLEDPGVKSVTKIYNYYKKFSYKTIVMGASFRNTGEIKALAGCDFLTISPKLLGELLQDNAKLVPVLSAKAAQASDLEKIHLDEKSFRWLHNEDQMAVEKLSDGIRKFAADAVKLERMLTERMFNAENGK 8CB4992AEF364E64 sh-l3-1 5 9 unspecified role unspecified role bait bait cub cub cub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey nubg nubg nubg undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association results sh_hrsv-rpl7-1 5 9 unspecified role unspecified role bait bait cub cub cub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey nubg nubg nubg undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association results sh_hrsv-rps13-1 5 9 unspecified role unspecified role bait bait cub cub cub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey nubg nubg nubg undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association results sh_hrsv-taldo1-1 5 9 unspecified role unspecified role bait bait cub cub cub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey nubg nubg nubg undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association results sh_hrsv-bcap31-1 5 9 unspecified role unspecified role bait bait cub cub cub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey nubg nubg nubg undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association results sh-bap31-1 6 8 unspecified role unspecified role neutral component neutral component c terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component n terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 1A bcap31-sh_hrsv-1 1 8 unspecified role unspecified role neutral component neutral component c terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component n terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 1B bcap31-sh_hrsv-2 4 8 unspecified role unspecified role bait bait his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey n terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2A bcap31-sh_hrsv-3 3 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey n terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2B bcap31-sh_hrsv-4 2 8 unspecified role unspecified role neutral component neutral component region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role neutral component neutral component purified purified 15n label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 3