UniProt UniProt http://www.uniprot.org Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. J. Immunol. (0022-1767) 2014 imex curation Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw Accepted 2014-DEC-03 by MGT J. Immunol. (0022-1767) 2014 Only protein-protein interactions imex curation Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. J. Immunol. (0022-1767) 2014 imex curation Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast lexa b52 complement lexa b52 complementation LexA B52 transcription complementation lexa b52 complement nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw Accepted 2014-DEC-03 by MGT J. Immunol. (0022-1767) 2014 Only protein-protein interactions imex curation Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. J. Immunol. (0022-1767) 2014 imex curation Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hmec-1 Human HMEC-1 immortalized microvascular endothelial cell line hmec-1 HMEC-1 - microvascular endothelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw Accepted 2014-DEC-03 by MGT mouse cDNA (not further specified) J. Immunol. (0022-1767) 2014 Only protein-protein interactions imex curation Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. Dengue viral protease interaction with NF-κB inhibitor α/β results in endothelial cell apoptosis and hemorrhage development. J. Immunol. (0022-1767) 2014 imex curation Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lin J.C., Lin S.C., Chen W.Y., Yen Y.T., Lai C.W., Tao M.H., Lin Y.L., Miaw S.C., Wu-Hsieh B.A. Virus - Publications including interactions involving viral proteins Origin of DENV as DEN-2 PL046 (based on the sequences of DEN-2 New Guinea-C strain, GenBank accession number M29095) has been confirmed by the authors. Coordinates of viral 'Pro' NS3-185 fragment according ref 24 (pmid 12867651) and NS2B-NS3 fragment following UniProt coordinates. d95449001@ntu.edu.tw Accepted 2014-DEC-03 by MGT J. Immunol. (0022-1767) 2014 Only protein-protein interactions imex curation polg_den2n Genome polyprotein protein protein den2n Dengue virus type 2 (strain Thailand/NGS-C/1944) (DENV-2) MNNQRKKARNTPFNMLKRERNRVSTVQQLTKRFSLGMLQGRGPLKLFMALVAFLRFLTIPPTAGILKRWGTIKKSKAINVLRGFRKEIGRMLNILNRRRRTAGMIIMLIPTVMAFHLTTRNGEPHMIVSRQEKGKSLLFKTEDGVNMCTLMAMDLGELCEDTITYKCPFLKQNEPEDIDCWCNSTSTWVTYGTCTTTGEHRREKRSVALVPHVGMGLETRTETWMSSEGAWKHAQRIETWILRHPGFTIMAAILAYTIGTTHFQRALIFILLTAVAPSMTMRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIETEAKQPATLRKYCIEAKLTNTTTDSRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFTCKKNMKGKVVQPENLEYTIVITPHSGEEHAVGNDTGKHGKEIKITPQSSITEAELTGYGTVTMECSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKVVKEIAETQHGTIVIRVQYEGDGSPCKIPFEIMDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIIGVEPGQLKLNWFKKGSSIGQMIETTMRGAKRMAILGDTAWDFGSLGGVFTSIGKALHQVFGAIYGAAFSGVSWIMKILIGVIITWIGMNSRSTSLSVSLVLVGVVTLYLGVMVQADSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWKQITPELNHILSENEVKLTIMTGDIKGIMQAGKRSLQPQPTELKYSWKTWGKAKMLSTESHNQTFLIDGPETAECPNTNRAWNSLEVEDYGFGVFTTNIWLKLREKQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKIEKASFIEVKSCHWPKSHTLWSNGVLESEMIIPKNFAGPVSQHNYRPGYHTQTAGPWHLGKLEMDFDFCEGTTVVVTEDCGNRGPSLRTTTASGKLITEWCCRSCTLPPLRYRGEDGCWYGMEIRPLKEKEENLVNSLVTAGHGQIDNFSLGVLGMALFLEEMLRTRVGTKHAILLVAVSFVTLITGNMSFRDLGRVMVMVGATMTDDIGMGVTYLALLAAFKVRPTFAAGLLLRKLTSKELMMTTIGIVLLSQSTIPETILELTDALALGMMVLKMVRKMEKYQLAVTIMAILCVPNAVILQNAWKVSCTILAVVSVSPLFLTSSQQKADWIPLALTIKGLNPTAIFLTTLSRTNKKRSWPLNEAIMAVGMVSILASSLLKNDIPMTGPLVAGGLLTVCYVLTGRSADLELERAADVKWEDQAEISGSSPILSITISEDGSMSIKNEEEEQTLTILIRTGLLVISGLFPVSIPITAAAWYLWEVKKQRAGVLWDVPSPPPVGKAELEDGAYRIKQKGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHKGKRIEPSWADVKKDLISYGGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTNAGTIGAVSLDFSPGTSGSPIIDKKGKVVGLYGNGVVTRSGAYVSAIAQTEKSIEDNPEIEDDIFRKRKLTIMDLHPGAGKTKRYLPAIVREAIKRGLRTLILAPTRVVAAEMEEALRGLPIRYQTPAIRAEHTGREIVDLMCHATFTMRLLSPVRVPNYNLIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIMDEEREIPERSWSSGHEWVTDFKGKTVWFVPSIKAGNDIAACLRKNGKKVIQLSRKTFDSEYVKTRTNDWDFVVTTDISEMGANFKAERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRIGRNPKNENDQYIYMGEPLENDEDCAHWKEAKMLLDNINTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYADRRWCFDGIKNNQILEENVEVEIWTKEGERKKLKPRWLDAKIYSDPLALKEFKEFAAGRKSLTLNLITEMGRLPTFMTQKARDALDNLAVLHTAEAGGRAYNHALSELPETLETLLLLTLLATVTGGIFLFLMSGRGIGKMTLGMCCIITASILLWYAQIQPHWIAASIILEFFLIVLLIPEPEKQRTPQDNQLTYVVIAILTVVAATMANEMGFLEKTKKDLGLGSITTQQPESNILDIDLRPASAWTLYAVATTFVTPMLRHSIENSSVNVSLTAIANQATVLMGLGKGWPLSKMDIGVPLLAIGCYSQVNPITLTAALFLLVAHYAIIGPGLQAKATREAQKRAAAGIMKNPTVDGITVIDLDPIPYDPKFEKQLGQVMLLVLCVTQVLMMRTTWALCEALTLATGPISTLWEGNPGRFWNTTIAVSMANIFRGSYLAGAGLLFSIMKNTTNTRRGTGNIGETLGEKWKSRLNALGKSEFQIYKKSGIQEVDRTLAKEGIKRGETDHHAVSRGSAKLRWFVERNMVTPEGKVVDLGCGRGGWSYYCGGLKNVREVKGLTKGGPGHEEPIPMSTYGWNLVRLQSGVDVFFTPPEKCDTLLCDIGESSPNPTVEAGRTLRVLNLVENWLNNNTQFCIKVLNPYMPSVIEKMEALQRKYGGALVRNPLSRNSTHEMYWLSNASGNIVSSVNMISRMLINRFTMRHKKATYEPDVDLGSGTRNIGIESEIPNLDIIGKRIEKIKQEHETSWHYDQDHPYKTWAYHGSYETKQTGSASSMGNGVVRLLTKPWDVVPMVTQMAMTDTTPFGQQRVFKEKVDTRTQEPKEGTKKLMKITAEWLWKELGKKKTPRMCTREEFTRKVRSNAALGAIFTDENKWKSAREAVEDSRFWELVDKERNLHLEGKCETCVYNMMGKREKKLGEFGKAKGSRAIWYMWLGARFLEFEALGFLNEDHWFSRENSLSGVEGEGLHKLGYILRDVSKKEGGAMYADDTAGWDTRITLEDLKNEEMVTNHMEGEHKKLAEAIFKLTYQNKVVRVQRPTPRGTVMDIISRRDQRGSGQVGTYGLNTFTNMEAQLIRQMEGEGVFKSIQHLTVTEEIAVQNWLARVGRERLSRMAISGDDCVVKPLDDRFASALTALNDMGKVRKDIQQWEPSRGWNDWTQVPFCSHHFHELIMKDGRVLVVPCRNQDELIGRARISQGAGWSLRETACLGKSYAQMWSLMYFHRRDLRLAANAICSAVPSHWVPTSRTTWSIHAKHEWMTTEDMLTVWNRVWIQENPWMEDKTPVESWEEIPYLGKREDQWCGSLIGLTSRATWAKNIQTAINQVRSLIGNEEYTDYMPSMKRFRKEEEEAGVLW FFF98B4FF5B4BC57 p14340-pro_0000037965 Serine protease NS3 protein protein den2n Dengue virus type 2 (strain Thailand/NGS-C/1944) (DENV-2) AGVLWDVPSPPPVGKAELEDGAYRIKQKGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHKGKRIEPSWADVKKDLISYGGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTNAGTIGAVSLDFSPGTSGSPIIDKKGKVVGLYGNGVVTRSGAYVSAIAQTEKSIEDNPEIEDDIFRKRKLTIMDLHPGAGKTKRYLPAIVREAIKRGLRTLILAPTRVVAAEMEEALRGLPIRYQTPAIRAEHTGREIVDLMCHATFTMRLLSPVRVPNYNLIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIMDEEREIPERSWSSGHEWVTDFKGKTVWFVPSIKAGNDIAACLRKNGKKVIQLSRKTFDSEYVKTRTNDWDFVVTTDISEMGANFKAERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRIGRNPKNENDQYIYMGEPLENDEDCAHWKEAKMLLDNINTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYADRRWCFDGIKNNQILEENVEVEIWTKEGERKKLKPRWLDAKIYSDPLALKEFKEFAAGRK 1476 2093 2E8AC52B66680775 ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL DIP protein P25963 original sequence version: 160 DIP protein P25963 original sequence version: 157 DIP protein P25963 original sequence version: 145 DIP protein P25963 original sequence version: 170 DIP protein P25963 original sequence version: 122 DIP protein P25963 original sequence version: 128 DIP protein P25963 original sequence version: 78 DIP protein P25963 original sequence version: 121 DIP protein P25963 original sequence version: 124 088B313226786395 ikkb_mouse Inhibitor of nuclear factor kappa-B kinase subunit beta I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Ikbkb Ikkb Serine/threonine protein kinase IKBKB protein protein mouse Mus musculus Mouse MSWSPSLPTQTCGAWEMKERLGTGGFGNVIRWHNQATGEQIAIKQCRQELSPKNRNRWCLEIQIMRRLNHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRRYLNQFENCCGLREGAVLTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEKRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGAVKFSSSLPFPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPQYGPNGCFRALDDILNLKLVHVLNMVTGTVHTYPVTEDESLQSLKTRIQENTGILETDQELLQKAGLVLLPDKPATQCISDSKTNEGLTLDMDLVFLLDNSKINYETQITPRPPPESVSCILQEPKRNLSFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMSLLRNNSCLSKMKNAMASTAQQLKAKLDFFKTSIQIDLEKYKEQTEFGITSDKLLLAWREMEQAVEQCGRENDVKHLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRKLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDERTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNVSRLSHPGQLMSQPSSACDSLPESDKKSEELVAEAHALCSRLESALQDTVKEQDRSFTTLDWSWLQMEDEERCSLEQACD DIP protein O88351 original sequence version: 126 DIP protein O88351 original sequence version: 73 DIP protein O88351 original sequence version: 99 DIP protein O88351 original sequence version: 114 DIP protein O88351 original sequence version: 149 FED962F095449C5E ikbb_human NF-kappa-B inhibitor beta NFKBIB IKBB TRIP9 I-kappa-B-beta Thyroid receptor-interacting protein 9 protein protein human Homo sapiens Human MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV E84575971B6F81CC polg-ikbkb 2 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait ns2b-ns3 protease complex sufficient to bind sufficient binding region sufficient to bind range range range range Uncleaved fragment including UniProt AC P14340 PRO_0000037964 and PRO_0000037965 physical association physical association no figure polg-nfkbia-2 4 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range pro ns3-185 fragment sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association 1B and S1D (right) A 2b-pro fragment (aa 1346-1660) with a NS3 his51ala mutation (corresponding to pos. 1526 in UniProt AC P14340) also interacts. p14340_pro-nfkbia 4 6 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role prey prey physical association physical association 1B polg-nfkbib-2 4 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range pro ns3-185 fragment sufficient to bind sufficient binding region sufficient to bind range range range range 9 unspecified role unspecified role prey prey physical association physical association 1D and S1F (right) A 2b-pro fragment (aa1346-1660) with a NS3 his51ala mutation (corresponding to pos. 1526 in UniProt AC P14340) also interacts. p14340_pro-nfkbib 4 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1D nfkbia-p14340_pro 3 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role bait bait physical association physical association 1F Bands corresponding to uncleaved NS2B-NS3 and cleaved NS3 detetcted (NS3 antibody). nfkbib-p14340_pro 3 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1F Bands corresponding to uncleaved NS2B-NS3 and cleaved NS3 detetcted (NS3 antibody). nfkbia-polg 1 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range pro ns3-185 fragment sufficient to bind sufficient binding region sufficient to bind range range range range 7 unspecified role unspecified role bait bait physical association physical association S1A and S1D (left) A 2B-Pro fragment with a NS3 his51ala mutation (corresponding pos. 1526 in UniProt AC P14340) also interacts. nfkbib-polg 1 9 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection ns2b-ns3 protease complex sufficient to bind sufficient binding region sufficient to bind range range range range Uncleaved fragment including UniProt AC P14340 PRO_0000037964 and PRO_0000037965 c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association S1B and S1F (left) A 2b-pro fragment (aa 1346-1660) with a NS3 his51ala mutation (corresponding to pos. 1526 in UniProt AC P14340) also interacts.