HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A. The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A. PLoS Pathog. (1553-7366) 2013 imex curation Reiss S., Harak C., Romero-Brey I., Radujkovic D., Klein R., Ruggieri A., Rebhan I., Bartenschlager R., Lohmann V. Virus - Publications including interactions involving viral proteins volker_lohmann@med.uni-heidelberg.de 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Reiss S., Harak C., Romero-Brey I., Radujkovic D., Klein R., Ruggieri A., Rebhan I., Bartenschlager R., Lohmann V. Virus - Publications including interactions involving viral proteins volker_lohmann@med.uni-heidelberg.de Accepted 2013-NOV-28 by ORCHARD PLoS Pathog. (1553-7366) 2013 imex curation The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A. The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A. PLoS Pathog. (1553-7366) 2013 imex curation Reiss S., Harak C., Romero-Brey I., Radujkovic D., Klein R., Ruggieri A., Rebhan I., Bartenschlager R., Lohmann V. Virus - Publications including interactions involving viral proteins volker_lohmann@med.uni-heidelberg.de 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Reiss S., Harak C., Romero-Brey I., Radujkovic D., Klein R., Ruggieri A., Rebhan I., Bartenschlager R., Lohmann V. Virus - Publications including interactions involving viral proteins volker_lohmann@med.uni-heidelberg.de Accepted 2013-NOV-28 by ORCHARD PLoS Pathog. (1553-7366) 2013 imex curation The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A. The lipid kinase phosphatidylinositol-4 kinase III alpha regulates the phosphorylation status of hepatitis C virus NS5A. PLoS Pathog. (1553-7366) 2013 imex curation Reiss S., Harak C., Romero-Brey I., Radujkovic D., Klein R., Ruggieri A., Rebhan I., Bartenschlager R., Lohmann V. Virus - Publications including interactions involving viral proteins volker_lohmann@med.uni-heidelberg.de 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Reiss S., Harak C., Romero-Brey I., Radujkovic D., Klein R., Ruggieri A., Rebhan I., Bartenschlager R., Lohmann V. Virus - Publications including interactions involving viral proteins volker_lohmann@med.uni-heidelberg.de Accepted 2013-NOV-28 by ORCHARD PLoS Pathog. (1553-7366) 2013 imex curation q9wmx2-pro_0000037551 Non-structural protein 5A protein protein hcvco Hepatitis C virus genotype 1b (isolate Con1) (HCV) SGSWLRDVWDWICTVLTDFKTWLQSKLLPRLPGVPFFSCQRGYKGVWRGDGIMQTTCPCGAQITGHVKNGSMRIVGPRTCSNTWHGTFPINAYTTGPCTPSPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLREEVTFLVGLNQYLVGSQLPCEPEPDVAVLTSMLTDPSHITAETAKRRLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFEPLQAEEDEREVSVPAEILRRSRKFPRAMPIWARPDYNPPLLESWKDPDYVPPVVHGCPLPPAKAPPIPPPRRKRTVVLSESTVSSALAELATKTFGSSESSAVDSGTATASPDQPSDDGDAGSDVESYSSMPPLEGEPGDPDLSDGSWSTVSEEASEDVVCC 1973 2419 AC8324B2DE0F7FAE pi4ka_human Phosphatidylinositol 4-kinase alpha PIK4CA PI4KA PIK4 Phosphatidylinositol 4-Kinase III alpha protein protein human Homo sapiens Human MAAAPARGGGGGGGGGGGCSGSGSSASRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLKGLPKVYWVEESTARKGRGALPVAESFSFCLVTLLSDVAYRDPSLRDEILEVLLQVLHVLLGMCQALEIQDKEYLCKYAIPCLIGISRAFGRYSNMEESLLSKLFPKIPPHSLRVLEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGMPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSVSPLFNGVTYKEFNIPLEMLRELLNLVKKIVEEAVLKSLDAIVASVMEANPSADLYYTSFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNTSQGELQKILHDADRIHNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGNDIKISVTNEHSESTLNVMSGKKSQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLSERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSGTTGQMSDLNKMMVQDLHSALDRSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY ED4C3C9EA79E6B5D q99ib8-pro_0000045602 Non-structural protein 5A protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCC 1977 2442 0BDEA498491643D8 ns5a-pi4k3alpha-1 2 5 unspecified role unspecified role bait bait radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS5A 6 unspecified role unspecified role prey prey Q99IB8-PRO_0000045602:p.Arg200_Met202delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain RSM AAA Thu Jul 07 12:16:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg200_ser201_met202ala Fri Jul 22 11:10:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg200ala,ser201ala,met202ala' Wed May 02 18:14:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_ser_met200-202ala_ala_ala' Tue Jun 30 20:13:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg200_Met202delinsAlaAlaAla' Q99IB8-PRO_0000045602:p.Thr204_Pro206delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TDP AAA Wed May 02 17:45:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr_asp_pro204-206ala_ala_ala' Tue Jun 30 20:03:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr204_Pro206delinsAlaAlaAla' Q99IB8-PRO_0000045602:p.Pro206_His208delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain PPH AAA Wed May 02 18:30:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro_his206-208ala_ala_ala' Tue Jun 30 16:33:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro206_His208delinsAlaAlaAla' Q99IB8-PRO_0000045602:p.Thr210_Glu212delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TAE AAA Wed May 02 16:45:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr_ala_glu210-212ala_ala_ala' Tue Jun 30 18:49:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr210_Glu212delinsAlaAlaAla' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q99IB8-PRO_0000045602:p.Cys190_Pro192delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain CEP AAA Wed May 02 18:06:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys_glu_pro190-192ala_ala_ala' Tue Jun 30 16:35:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys190_Pro192delinsAlaAlaAla' Q99IB8-PRO_0000045602:p.Met202_Thr204delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain MLT AAA Wed May 02 15:34:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met_leu_thr202-204ala_ala_ala' Tue Jun 30 18:26:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met202_Thr204delinsAlaAlaAla' Q99IB8-PRO_0000045602:p.His208_Thr210delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain HIT AAA Wed May 02 15:40:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his_ile_thr208-210ala_ala_ala' Tue Jun 30 19:45:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His208_Thr210delinsAlaAlaAla' physical association physical association Fig. 1, 2 and table 1 q99ib8_pro-pi4ka-2 1 NS5A 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4A pi4ka-q99ib8_pro-2 3 5 unspecified role unspecified role prey prey radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS5A 6 unspecified role unspecified role bait bait radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6, 7, S4 and S3F All mutations in the PFIS caused an enhancement of p58/p56 levels and decreased binding to PI4KIIIa q99ib8_pro-pi4ka-1 3 NS5A 4 unspecified role unspecified role bait bait radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey P42356:p.Trp2015Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W A Fri Jul 22 11:10:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'trp1957ala' Wed May 02 16:33:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp2015ala' Tue Jun 30 15:38:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp2015Ala' radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 7 The HIT mutation in Con1 resulted in a significantly increased p58/p56 ratio and was associated with a strongly decreased binding to PI4KIIIa.