HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. J. Virol. (0022-538X) 2013 imex curation Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw Accepted 2013-DEC-12 by ORCHARD J. Virol. (0022-538X) 2013 imex curation Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. J. Virol. (0022-538X) 2013 imex curation Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw Accepted 2013-DEC-12 by ORCHARD J. Virol. (0022-538X) 2013 imex curation Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. J. Virol. (0022-538X) 2013 imex curation Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw Accepted 2013-DEC-12 by ORCHARD J. Virol. (0022-538X) 2013 imex curation Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. J. Virol. (0022-538X) 2013 imex curation Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw Accepted 2013-DEC-12 by ORCHARD J. Virol. (0022-538X) 2013 imex curation Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. J. Virol. (0022-538X) 2013 imex curation Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells confocal microscopy confocal microscopy immunostaining immunostaining Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw Accepted 2013-DEC-12 by ORCHARD J. Virol. (0022-538X) 2013 imex curation Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. Hepatitis C virus replication is modulated by the interaction of nonstructural protein NS5B and fatty acid synthase. J. Virol. (0022-538X) 2013 imex curation Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang J.T., Tseng C.P., Liao M.H., Lu S.C., Yeh W.Z., Sakamoto N., Chen C.M., Cheng J.C. Virus - Publications including interactions involving viral proteins chchen1@dragon.nchu.edu.tw Accepted 2013-DEC-12 by ORCHARD J. Virol. (0022-538X) 2013 imex curation fas_human Fatty acid synthase FASN FAS Type I fatty acid synthase protein protein human Homo sapiens Human MEEVVIAGMSGKLPESENLQEFWDNLIGGVDMVTDDDRRWKAGLYGLPRRSGKLKDLSRFDASFFGVHPKQAHTMDPQLRLLLEVTYEAIVDGGINPDSLRGTHTGVWVGVSGSETSEALSRDPETLVGYSMVGCQRAMMANRLSFFFDFRGPSIALDTACSSSLMALQNAYQAIHSGQCPAAIVGGINVLLKPNTSVQFLRLGMLSPEGTCKAFDTAGNGYCRSEGVVAVLLTKKSLARRVYATILNAGTNTDGFKEQGVTFPSGDIQEQLIRSLYQSAGVAPESFEYIEAHGTGTKVGDPQELNGITRALCATRQEPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGLWAPNLHFHSPNPEIPALLDGRLQVVDQPLPVRGGNVGINSFGFGGSNVHIILRPNTQPPPAPAPHATLPRLLRASGRTPEAVQKLLEQGLRHSQDLAFLSMLNDIAAVPATAMPFRGYAVLGGERGGPEVQQVPAGERPLWFICSGMGTQWRGMGLSLMRLDRFRDSILRSDEAVKPFGLKVSQLLLSTDESTFDDIVHSFVSLTAIQIGLIDLLSCMGLRPDGIVGHSLGEVACGYADGCLSQEEAVLAAYWRGQCIKEAHLPPGAMAAVGLSWEECKQRCPPGVVPACHNSKDTVTISGPQAPVFEFVEQLRKEGVFAKEVRTGGMAFHSYFMEAIAPPLLQELKKVIREPKPRSARWLSTSIPEAQWHSSLARTSSAEYNVNNLVSPVLFQEALWHVPEHAVVLEIAPHALLQAVLKRGLKPSCTIIPLMKKDHRDNLEFFLAGIGRLHLSGIDANPNALFPPVEFPAPRGTPLISPLIKWDHSLAWDVPAAEDFPNGSGSPSAAIYNIDTSSESPDHYLVDHTLDGRVLFPATGYLSIVWKTLARALGLGVEQLPVVFEDVVLHQATILPKTGTVSLEVRLLEASRAFEVSENGNLVVSGKVYQWDDPDPRLFDHPESPTPNPTEPLFLAQAEVYKELRLRGYDYGPHFQGILEASLEGDSGRLLWKDNWVSFMDTMLQMSILGSAKHGLYLPTRVTAIHIDPATHRQKLYTLQDKAQVADVVVSRWLRVTVAGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSERAALQEELQLCKGLVQALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRLLSAACRLQLNGNLQLELAQVLAQERPKLPEDPLLSGLLDSPALKACLDTAVENMPSLKMKVVEVLAGHGHLYSRIPGLLSPHPLLQLSYTATDRHPQALEAAQAELQQHDVAQGQWDPADPAPSALGSADLLVCNCAVAALGDPASALSNMVAALREGGFLLLHTLLRGHPLGDIVAFLTSTEPQYGQGILSQDAWESLFSRVSLRLVGLKKSFYGSTLFLCRRPTPQDSPIFLPVDDTSFRWVESLKGILADEDSSRPVWLKAINCATSGVVGLVNCLRREPGGNRLRCVLLSNLSSTSHVPEVDPGSAELQKVLQGDLVMNVYRDGAWGAFRHFLLEEDKPEEPTAHAFVSTLTRGDLSSIRWVCSSLRHAQPTCPGAQLCTVYYASLNFRDIMLATGKLSPDAIPGKWTSQDSLLGMEFSGRDASGKRVMGLVPAKGLATSVLLSPDFLWDVPSNWTLEEAASVPVVYSTAYYALVVRGRVRPGETLLIHSGSGGVGQAAIAIALSLGCRVFTTVGSAEKRAYLQARFPQLDSTSFANSRDTSFEQHVLWHTGGKGVDLVLNSLAEEKLQASVRCLATHGRFLEIGKFDLSQNHPLGMAIFLKNVTFHGVLLDAFFNESSADWREVWALVQAGIRDGVVRPLKCTVFHGAQVEDAFRYMAQGKHIGKVVVQVLAEEPEAVLKGAKPKLMSAISKTFCPAHKSYIIAGGLGGFGLELAQWLIQRGVQKLVLTSRSGIRTGYQAKQVRRWRRQGVQVQVSTSNISSLEGARGLIAEAAQLGPVGGVFNLAVVLRDGLLENQTPEFFQDVCKPKYSGTLNLDRVTREACPELDYFVVFSSVSCGRGNAGQSNYGFANSAMERICEKRRHEGLPGLAVQWGAIGDVGILVETMSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG 7A07171FEFA3287B q99ib8-pro_0000045603 RNA-directed RNA polymerase protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SMSYSWTGALITPCSPEEEKLPINPLSNSLLRYHNKVYCTTSKSASQRAKKVTFDRTQVLDAHYDSVLKDIKLAASKVSARLLTLEEACQLTPPHSARSKYGFGAKEVRSLSGRAVNHIKSVWKDLLEDPQTPIPTTIMAKNEVFCVDPAKGGKKPARLIVYPDLGVRVCEKMALYDITQKLPQAVMGASYGFQYSPAQRVEYLLKAWAEKKDPMGFSYDTRCFDSTVTERDIRTEESIYQACSLPEEARTAIHSLTERLYVGGPMFNSKGQTCGYRRCRASGVLTTSMGNTITCYVKALAACKAAGIVAPTMLVCGDDLVVISESQGTEEDERNLRAFTEAMTRYSAPPGDPPRPEYDLELITSCSSNVSVALGPRGRRRYYLTRDPTTPLARAAWETVRHSPINSWLGNIIQYAPTIWVRMVLMTHFFSILMVQDTLDQNLNFEMYGSVYSVNPLDLPAIIERLHGLDAFSMHTYSHHELTRVASALRKLGAPPLRVWKSRARAVRASLISRGGKAAVCGRYLFNWAVKTKLKLTPLPEARLLDLSSWFTVGAGGGDIFHSVSRARPRSLLFGLLLLFVGVGLFLLPAR 2443 3033 92FA1DE7EA9B8DBC ns5b-fasn-3 3 NS5B 8 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) FASN 7 unspecified role unspecified role prey prey human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma physical association physical association Fig. 1A ns5b-fasn-1 4 NS5B 8 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) FASN 7 unspecified role unspecified role prey prey human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma physical association physical association Fig. 1B fasn-ns5b-1 1 FASN 7 unspecified role unspecified role bait bait NS5B 8 unspecified role unspecified role prey prey physical association physical association Fig. 3B fasn-ns5b-2 6 NS5B 8 unspecified role unspecified role prey prey FASN 7 unspecified role unspecified role bait bait physical association physical association Fig. 3C ns5b-fasn-4 5 FASN 7 unspecified role unspecified role neutral component neutral component NS5B 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3D ns5b-fasn-2 2 FASN 7 unspecified role unspecified role neutral component neutral component NS5B 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3D