IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. J. Virol. (0022-538X) 2012 imex curation Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu Accepted 2013-SEP-10 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. J. Virol. (0022-538X) 2012 imex curation Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed confocal microscopy confocal microscopy immunostaining immunostaining Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu Accepted 2013-SEP-10 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. J. Virol. (0022-538X) 2012 imex curation Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hep2 Homo sapiens hep2 hep2 confocal microscopy confocal microscopy immunostaining immunostaining Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu Accepted 2013-SEP-10 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. J. Virol. (0022-538X) 2012 imex curation Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Affinomics - Interactions curated for the Affinomics consortium. Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu Accepted 2013-SEP-10 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation phosp_hrsva Phosphoprotein P protein protein hrsva human respiratory sy MEKFAPEFHGEDANNRATKFLESIKGKFTSPKDPKKKDSIISVNSIDIEVTKESPITSNSTIINPTNETDDTAGNKPNYQRKPLVSFKEDPTPSDNPFSKLYKETIETFDNNEEESSYSYEEINDQTNDNITARLDRIDEKLSEILGMLHTLVVASAGPTSARDGIRDAMIGLREEMIEKIRTEALMTNDRLEAMARLRNEESEKMAKDTSDEVSLNPTSEKLNNLLEGNDSDNDLSLEDF 21A9E45CA2DFD50C mavs_human Mitochondrial antiviral-signaling protein Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter CARD adapter inducing interferon beta Putative NF-kappa-B-activating protein 031N MAVS IPS1 KIAA1271 VISA protein protein human Homo sapiens Human MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH 0E23E3E115941EE8 m1qcq2_chlae MAVS protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFSTLQRRPGWVESFIAALRDCELADLADEVACVYQSYQPRTSDRPPDPLEPPSLPAEGPGPPTPAAAHSIPYNSYREKEPSYPMPVQETRAPDSPGESSEQAPQILNPRVIPRSPDGGPLDPSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPAGSVVSTGTSSSSSSSPGLASAGAAEGEQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVAPKVPANPASASTVPSKLPTSSKPPGAVPSVFTNPAPSKLPINSTRAGMVPSKVPTSMVRTKVSASTVPTDRSSRTEETPAAPTPAGATGGRSAWLDSSSENGGFESELSKPGMLVSQADSQFSGCSEDLAISASTSLGMGSCHGPEENEYKSEGTFGIHVAENPSIQLMEGNPGPPAGPQGGPRPHVDQKFQKREVPCHRPSPGALWLQAAVAGVLVVTLLVAMYRRRLH A47A9CD4AD9BF810 ifih1_chlae_protein Similar to Interferon-induced helicase C domain-containing protein 1 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to human Q9BYX4. ddx58_human Antiviral innate immune response receptor RIG-I DEAD box protein 58 Retinoic acid-inducible gene 1 protein DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid-inducible gene I protein RIG-I-like receptor 1 protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK BF0D501C395BAE25 ncap_hrsva Nucleoprotein N Nucleocapsid protein protein protein hrsva human respiratory sy MALSKVKLNDTLNKDQLLSSSKYTIQRSTGDSIDTPNYDVQKHINKLCGMLLITEDANHKFTGLIGMLYAMSRLGREDTIKILRDAGYHVKANGVDVTTHRQDINGKEMKFEVLTLASLTTEIQINIEIESRKSYKKMLKEMGEVAPEYRHDSPDCGMIILCIAALVITKLAAGDRSGLTAVIRRANNVLKNEMKRYKGLLPKDIANSFYEVFEKHPHFIDVFVHFGIAQSSTRGGSRVEGIFAGLFMNAYGAGQVMLRWGVLAKSVKNIMLGHASVQAEMEQVVEVYEYAQKLGGEAGFYHILNNPKASLLSLTQFPHFSSVVLGNAAGLGIMGEYRGTPRNQDLYDAAKAYAEQLKENGVINYSVLDLTAEELEAIKHQLNPKDNDVEL D06E84F4F88D382B ifih1_human Interferon-induced helicase C domain-containing protein 1 Helicase with 2 CARD domains Interferon-induced with helicase C domain protein 1 Melanoma differentiation-associated protein 5 Murabutide down-regulated protein RNA helicase-DEAD box protein 116 RIG-I-like receptor 2 IFIH1 MDA5 RH116 Clinically amyopathic dermatomyositis autoantigen 140 kDa protein protein human Homo sapiens Human MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDEENVAARASPEPELQLRPYQMEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKKASEPGKVIVLVNKVLLVEQLFRKEFQPFLKKWYRVIGLSGDTQLKISFPEVVKSCDIIISTAQILENSLLNLENGEDAGVQLSDFSLIIIDECHHTNKEAVYNNIMRHYLMQKLKNNRLKKENKPVIPLPQILGLTASPGVGGATKQAKAEEHILKLCANLDAFTIKTVKENLDQLKNQIQEPCKKFAIADATREDPFKEKLLEIMTRIQTYCQMSPMSDFGTQPYEQWAIQMEKKAAKEGNRKERVCAEHLRKYNEALQINDTIRMIDAYTHLETFYNEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED DIP protein Q9BYX4 original sequence version: 130 DIP protein Q9BYX4 original sequence version: 120 DIP protein Q9BYX4 original sequence version: 123 DIP protein Q9BYX4 original sequence version: 66 DIP protein Q9BYX4 original sequence version: 82 DIP protein Q9BYX4-1 original sequence version: 136 DIP protein Q9BYX4-1 original sequence version: 107 DIP protein Q9BYX4 original sequence version: 122 789CFB4824B92DC9 ifih1-mavs 1 6 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 3 Interaction found in viral inclusion bodies upon RSV infection. ddx58-mavs-7 1 9 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 2 Interaction found in viral inclusion bodies upon RSV infection. ifih1-n-1 3 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 4 Interaction found in viral filaments upon RSV infection. ifih1_chlae_protei-n 2 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 The cell were co-transfected with RSV P. mavs-n-3 2 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 The cell were co-transfected with RSV P. ifih1_chlae_prot-n-1 4 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 7 Interaction found in viral inclusion bodies induced upon RSV infection. mavs-n-3 4 7 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 7 Interaction found in viral inclusion bodies induced upon RSV infection. ifih1_chlae_prot-n-2 4 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 9 If the cells are co-transfected with RSV P, the interaction is found in inclusion bodies. mavs-n-3 4 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 9 If the cells are co-transfected with RSV P, the interaction is found in inclusion bodies. mda5-n-3 4 5 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role ancillary ancillary 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 13 Interaction found in inclusion bodies. p-n 2 5 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 16 The cell were co-transfected with RSV P.