UniProt UniProt http://www.uniprot.org DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. J. Infect. Dis. (0022-1899) 2011 imex curation Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins chris.basler@mssm.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins chris.basler@mssm.educ chris.basler@mssm.edu Accepted 2014-OCT-20 by ORCHARD J. Infect. Dis. (0022-1899) 2011 imex curation DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. J. Infect. Dis. (0022-1899) 2011 imex curation Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins chris.basler@mssm.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Incubation with cell lysates in presene or absence of polyI:C (structurally similar to double-stranded RNA) chris.basler@mssm.educ chris.basler@mssm.edu Accepted 2014-OCT-20 by ORCHARD J. Infect. Dis. (0022-1899) 2011 imex curation DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. J. Infect. Dis. (0022-1899) 2011 imex curation Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins chris.basler@mssm.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins Incubation with cell lysates in presene of polyI:C (structurally similar to double-stranded RNA) chris.basler@mssm.educ chris.basler@mssm.edu Accepted 2014-OCT-20 by ORCHARD J. Infect. Dis. (0022-1899) 2011 imex curation DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. DRBP76 associates with Ebola virus VP35 and suppresses viral polymerase function. J. Infect. Dis. (0022-1899) 2011 imex curation Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins chris.basler@mssm.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shabman R.S., Leung D.W., Johnson J., Glennon N., Gulcicek E.E., Stone K.L., Leung L., Hensley L., Amarasinghe G.K., Basler C.F. Virus - Publications including interactions involving viral proteins chris.basler@mssm.educ chris.basler@mssm.edu Accepted 2014-OCT-20 by ORCHARD J. Infect. Dis. (0022-1899) 2011 imex curation ncap_ebozm Nucleoprotein NP Nucleocapsid protein Ebola NP protein protein ebozm Zaire ebolavirus (strain Mayinga-76) ZEBOV Zaire Ebola virus MDSRPQKIWMAPSLTESDMDYHKILTAGLSVQQGIVRQRVIPVYQVNNLEEICQLIIQAFEAGVDFQESADSFLLMLCLHHAYQGDYKLFLESGAVKYLEGHGFRFEVKKRDGVKRLEELLPAVSSGKNIKRTLAAMPEEETTEANAGQFLSFASLFLPKLVVGEKACLEKVQRQIQVHAEQGLIQYPTAWQSVGHMMVIFRLMRTNFLIKFLLIHQGMHMVAGHDANDAVISNSVAQARFSGLLIVKTVLDHILQKTERGVRLHPLARTAKVKNEVNSFKAALSSLAKHGEYAPFARLLNLSGVNNLEHGLFPQLSAIALGVATAHGSTLAGVNVGEQYQQLREAATEAEKQLQQYAESRELDHLGLDDQEKKILMNFHQKKNEISFQQTNAMVTLRKERLAKLTEAITAASLPKTSGHYDDDDDIPFPGPINDDDNPGHQDDDPTDSQDTTIPDVVVDPDDGSYGEYQSYSENGMNAPDDLVLFDLDEDDEDTKPVPNRSTKGGQQKNSQKGQHIEGRQTQSRPIQNVPGPHRTIHHASAPLTDNDRRNEPSGSTSPRMLTPINEEADPLDDADDETSSLPPLESDDEEQDRDGTSNRTPTVAPPAPVYRDHSEKKELPQDEQQDQDHTQEARNQDSDNTQSEHSFEEMYRHILRSQGPFDAVLYYHMMKDEPVVFSTSDGKEYTYPDSLEEEYPPWLTEKEAMNEENRFVTLDGQQFYWPVMNHKNKFMAILQHHQ 159C254EA0478886 ilf3_human Interleukin enhancer-binding factor 3 ILF3 MPHOSPH4 NF90 Nuclear factor of activated T-cells 90 kDa Double-stranded RNA-binding protein 76 Translational control protein 80 Nuclear factor associated with dsRNA M-phase phosphoprotein 4 DRBF protein protein human Homo sapiens Human MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVEAVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANKKKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEVPPPPNLRGRGRGGSIRGRGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSQFYSNGGHSGNASGGGGGGGGGSSGYGSYYQGDNYNSPVPPKHAGKKQPHGGQQKPSYGSGYQSHQGQQQSYNQSPYSNYGPPQGKQKGYNHGQGSYSYSNSYNSPGGGGGSDYNYESKFNYSGSGGRSGGNSYGSGGASYNPGSHGGYGGGSGGGSSYQGKQGGYSQSNYNSPGSGQNYSGPPSSYQSSQGGYGRNADHSMNYQYR DIP protein Q12906 original sequence version: 87 DIP protein Q12906 original sequence version: 149 20903ABD0331F370 vp35_ebozm Polymerase cofactor VP35 VP35 Ebola VP35 protein protein ebozm Zaire ebolavirus (strain Mayinga-76) ZEBOV Zaire Ebola virus MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIFCDIENNPGLCYASQMQQTKPNPKTRNSQTQTDPICNHSFEEVVQTLASLATVVQQQTIASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVAKYDLLVMTTGRATATAAATEAYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLNSTTSLTEENFGKPDISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCALIQITKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGKTLGLKI DIP protein Q05127 original sequence version: 43 B171C7FDBB5B1FBD nucl_human Nucleolin NCL Protein C23 protein protein human Homo sapiens Human MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE DIP protein P19338 original sequence version: 93 DIP protein P19338 original sequence version: 123 C97F6E34E5CA6727 dhx9_human ATP-dependent RNA helicase A DHX9 DDX9 NDH2 DExH-box helicase 9 RNA helicase A LKP Nuclear DNA helicase II DEAH box protein 9 Leukophysin protein protein human Homo sapiens Human MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPPLTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGETVEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTIAAATCFPEPFINEGKRLGYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQVFTNTGPDNNLDVVISLLAFGVYPNVCYHKEKRKILTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGEKIRTRAISAKGMTLVTPLQLLLFASKKVQSDGQIVLVDDWIKLQISHEAAACITGLRAAMEALVVEVTKQPAIISQLDPVNERMLNMIRQISRPSAAGINLMIGSTRYGDGPRPPKMARYDNGSGYRRGGSSYSGGGYGGGYSSGGYGSGGYGGSANSFRAGYGAGVGGGYRGVSRGGFRGNSGGDYRGPSGGYRGSGGFQRGGGRGAYGTGYFGQGRGGGGY DIP protein Q08211 original sequence version: 114 DIP protein Q08211 original sequence version: 177 A607DA8F4C4B217A vp35-dhx9 3 6 unspecified role unspecified role prey prey cell lysate cell lysate human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role prey prey cell lysate cell lysate human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role prey prey cell lysate cell lysate human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait Q05127:p.Arg312Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:50:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg312ala' Tue Jun 30 18:26:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg312Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q05127:p.Lys339Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:50:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys339ala' Tue Jun 30 18:17:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys339Ala' ecoli Escherichia coli (strain K12) association association 1B Prey proteins specifically detected in presence of polyI:C . vp35-ilf3 2 6 unspecified role unspecified role prey prey cell lysate cell lysate human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait Q05127:p.Lys339Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:41:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys339ala' Tue Jun 30 16:14:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys339Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q05127:p.Arg312Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:21:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg312ala' Tue Jun 30 18:18:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg312Ala' region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) Association detected in absence of dsRNA (pI:C) physical association physical association 1D and 2B Interaction specifically detected in presence of polyI:C . VP35 mutant arg312ala does not interact neither in presence or absence of polyI:C. vp35-ilf3-1 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2D vp35-np-1 1 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 6B Presence of overexpressed host Flag-ILF3/DRBP76 (UniProt Ac Q12906) disrupts the association. vp35-ilf3-2 2 6 unspecified role unspecified role prey prey cell lysate cell lysate human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) Association detected in absence of dsRNA (pI:C) physical association physical association 2B Interaction specifically detected in absence of polyI:C . vp35-ilf3-3 2 7 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q05127:p.Arg312Ala mutation mutation certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:46:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg312ala' Tue Jun 30 19:51:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg312Ala' ecoli Escherichia coli (strain K12) Association detected in absence of dsRNA (pI:C) 6 unspecified role unspecified role prey prey cell lysate cell lysate human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2C Interaction specifically detected in absence of polyI:C . vp35-ilf3-4 2 7 unspecified role unspecified role bait bait Q05127:p.Lys339Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:23:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys339ala' Tue Jun 30 19:21:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys339Ala' region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q05127:p.Arg322Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:45:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg322ala' Tue Jun 30 18:22:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg322Ala' ecoli Escherichia coli (strain K12) Association detected in absence of dsRNA (pI:C) 6 unspecified role unspecified role prey prey cell lysate cell lysate human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 2C Interaction specifically detected in absence of polyI:C . Interaction is weaker compared to that full length VP35 carrying a arg312ala mutation. Mutations VP35 lys248ala and lys251ala do not change the association