IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. PLoS pathogens 2011 imex curation Decroly E., Debarnot C., Ferron F., Bouvet M., Coutard B., Imbert I., Gluais L., Papageorgiou N., Sharff A., Bricogne G., Ortiz-Lombardia M., Lescar J., Canard B. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus etienne.decroly@afmb.univ-mrs.fr,bruno.canard@afmb.univ-mrs.fr Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Decroly E., Debarnot C., Ferron F., Bouvet M., Coutard B., Imbert I., Gluais L., Papageorgiou N., Sharff A., Bricogne G., Ortiz-Lombardia M., Lescar J., Canard B. Virus - Publications including interactions involving viral proteins etienne.decroly@afmb.univ-mrs.fr,bruno.canard@afmb.univ-mrs.fr Accepted 2020-MAY-18 AT 08:37 BST AT 08:37 BST by MGT PLoS pathogens 2011 Only protein-protein interactions imex curation imex curation all changes done.. Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. PLoS pathogens 2011 imex curation Decroly E., Debarnot C., Ferron F., Bouvet M., Coutard B., Imbert I., Gluais L., Papageorgiou N., Sharff A., Bricogne G., Ortiz-Lombardia M., Lescar J., Canard B. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus etienne.decroly@afmb.univ-mrs.fr,bruno.canard@afmb.univ-mrs.fr Only protein-protein interactions imex curation in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as autoradiography autoradiography Decroly E., Debarnot C., Ferron F., Bouvet M., Coutard B., Imbert I., Gluais L., Papageorgiou N., Sharff A., Bricogne G., Ortiz-Lombardia M., Lescar J., Canard B. Virus - Publications including interactions involving viral proteins etienne.decroly@afmb.univ-mrs.fr,bruno.canard@afmb.univ-mrs.fr Accepted 2020-MAY-18 AT 08:37 BST AT 08:37 BST by MGT PLoS pathogens 2011 Only protein-protein interactions imex curation imex curation Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. PLoS pathogens 2011 imex curation Decroly E., Debarnot C., Ferron F., Bouvet M., Coutard B., Imbert I., Gluais L., Papageorgiou N., Sharff A., Bricogne G., Ortiz-Lombardia M., Lescar J., Canard B. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus etienne.decroly@afmb.univ-mrs.fr,bruno.canard@afmb.univ-mrs.fr Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Decroly E., Debarnot C., Ferron F., Bouvet M., Coutard B., Imbert I., Gluais L., Papageorgiou N., Sharff A., Bricogne G., Ortiz-Lombardia M., Lescar J., Canard B. Virus - Publications including interactions involving viral proteins etienne.decroly@afmb.univ-mrs.fr,bruno.canard@afmb.univ-mrs.fr Accepted 2020-MAY-18 AT 08:37 BST AT 08:37 BST by MGT PLoS pathogens 2011 Only protein-protein interactions imex curation imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method p0c6x7-pro_0000037322 2'-O-methyltransferase nsp16 ORF1ab polyprotein 1a-1b rep protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus ASQAWQPGVAMPNLYKMQRMLLEKCDLQNYGENAVIPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPRTKHVTKENDSKEGFFTYLCGFIKQKLALGGSIAVKITEHSWNADLYKLMGHFSWWTAFVTNVNASSSEAFLIGANYLGKPKEQIDGYTMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKENQINDMIYSLLEKGRLIIRENNRVVVSSDILVNN 6776 7073 14B6660399D3A801 s-adenosyl-l-homocysteine S-adenosyl-l-homocysteine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p0c6x7-pro_0000037317 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus AGNATEVPANSTVLSFCAFAVDPAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLRNTVCTVCGMWKGYGCSCDQLREPLMQ 4231 4369 1F48DD3A6874826C sinefungin sinefungin (2S,5S)-2,5-diamino-6-[(2R,3S,R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]hexanoic acid 6,9-Diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxy-beta-D-ribo-decofuranuronic acid 6,9-Diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxydecofuranuronic acid A 9145 ADENOSYL-ORNITHINE Antibiotic A 9145 sinefungin sinefungina sinefungine sinefunginum (5S)-5-amino-6-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]-L-norleucine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) LMXOHSDXUQEUSF-YECHIGJVSA-N LMXOHSDXUQEUSF-YECHIGJVSA-N s-adenosyl-l-methionine S adenosyl Methionine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 7meg5'-ppp5'accccc 7MeG5'-ppp5'ACCCCC small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rep-rep-65 3 5 unspecified role unspecified role neutral component neutral component purified purified n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component purified purified n-terminal region strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 cofactor cofactor coenzyme neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 2Å Rwork: 0.213 Rfree: 0.227 rep-rep-66 3 8 cofactor cofactor coenzyme neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component purified purified n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component purified purified n-terminal region strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 2.5Å Rwork: 0.201 Rfree: 0.235 rep-rep-67 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P0C6X7-PRO_0000037322:p.Arg86Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 19:51:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg86Ala' P0C6X7-PRO_0000037322:p.Val78Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V A Tue Apr 28 10:56:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Vla78Ala' Tue Jun 30 17:15:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78Ala' P0C6X7-PRO_0000037322:p.Gln87Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q A Tue Jun 30 16:58:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln87Ala' P0C6X7-PRO_0000037322:p.Val104Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V G Tue Jun 30 17:48:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val104Gly' P0C6X7-PRO_0000037322:p.Asp106Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 17:43:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp106Ala' P0C6X7-PRO_0000037322:p.Leu244Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 20:01:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu244Ala' P0C6X7-PRO_0000037322:p.Met247Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 18:18:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met247Ala' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0C6X7-PRO_0000037322:p.Lys46Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 20:12:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys46Ala' P0C6X7-PRO_0000037322:p.Asp130Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 18:26:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp130Ala' P0C6X7-PRO_0000037322:p.Lys170Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:18:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys170Ala' P0C6X7-PRO_0000037322:p.Glu203Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 16:10:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu203Ala' P0C6X7-PRO_0000037322:p.Asn43Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 18:01:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn43Ala' P0C6X7-PRO_0000037322:p.Gly73Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 18:45:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly73Ala' P0C6X7-PRO_0000037322:p.Asp99Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 16:03:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp99Ala' P0C6X7-PRO_0000037322:p.Thr58Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 17:52:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Ala' P0C6X7-PRO_0000037322:p.Thr58Asn mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T N Tue Jun 30 18:12:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Asn' P0C6X7-PRO_0000037322:p.Thr58Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T E Tue Jun 30 16:04:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Glu' P0C6X7-PRO_0000037322:p.Ser188Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 18:55:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser188Ala' P0C6X7-PRO_0000037322:p.Tyr132Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 16:04:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132Ala' P0C6X7-PRO_0000037322:p.Tyr132Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y T Tue Jun 30 19:17:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132Thr' P0C6X7-PRO_0000037322:p.Tyr132Phe mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 18:58:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132Phe' P0C6X7-PRO_0000037322:p.Tyr132His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y H Tue Jun 30 19:05:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132His' P0C6X7-PRO_0000037322:p.Tyr30Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 17:52:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr30Ala' P0C6X7-PRO_0000037322:p.Tyr30Phe mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 17:09:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr30Phe' P0C6X7-PRO_0000037322:p.Ile40Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 15:39:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile40Ala' P0C6X7-PRO_0000037322:p.Met41Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 20:16:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met41Ala' P0C6X7-PRO_0000037322:p.Val44Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V A Tue Jun 30 18:54:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val44Ala' P0C6X7-PRO_0000037322:p.Thr48Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 15:37:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr48Ala' 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P0C6X7-PRO_0000037317:p.Asn40Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 19:20:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn40Ala' P0C6X7-PRO_0000037317:p.Val42Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V A Tue Jun 30 15:49:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Ala' P0C6X7-PRO_0000037317:p.Met44Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 17:15:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met44Ala' P0C6X7-PRO_0000037317:p.Leu45Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 20:11:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu45Ala' P0C6X7-PRO_0000037317:p.Thr47Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 16:52:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr47Ala' P0C6X7-PRO_0000037317:p.Thr58Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 17:45:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Ala' P0C6X7-PRO_0000037317:p.Gly69Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 20:22:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly69Ala' P0C6X7-PRO_0000037317:p.Gly70Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 18:19:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly70Ala' P0C6X7-PRO_0000037317:p.Ser72Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A Tue Jun 30 19:39:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser72Ala' P0C6X7-PRO_0000037317:p.Arg78Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 19:20:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Ala' P0C6X7-PRO_0000037317:p.His80Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Tue Jun 30 17:20:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His80Ala' P0C6X7-PRO_0000037317:p.Lys93Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 18:41:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Ala' P0C6X7-PRO_0000037317:p.Gly94Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 17:45:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' P0C6X7-PRO_0000037317:p.Lys95Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:07:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys95Ala' P0C6X7-PRO_0000037317:p.Tyr96Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 17:20:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ala' n-terminus strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Tables 2, S1, S2 rep-rep-71 3 5 unspecified role unspecified role neutral component neutral component purified purified n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component purified purified P0C6X7-PRO_0000037317:p.Tyr96Phe mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 17:18:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Phe' n-terminal region strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 cofactor cofactor coenzyme neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 2.06Å Rwork: 0.20 Rfree: 0.234 7meg5'_ppp5'accccc-nsp10_nsp16_cvhsa-1 2 9 donor donor neutral component neutral component region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 10 acceptor acceptor neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 83 enzyme enzyme neutral component neutral component purified purified P0C6X7-PRO_0000037322:p.Arg86Ala P0C6X7-PRO_0000037322:p.Arg86Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain R A 85 Mon Jun 29 10:00:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Arg86Ala' Tue Jun 30 20:20:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg86Ala' P0C6X7-PRO_0000037322:p.Val78Ala P0C6X7-PRO_0000037322:p.Val78Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V A 85 Mon Jun 29 10:26:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Val78Ala' Tue Jun 30 15:57:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78Ala' P0C6X7-PRO_0000037322:p.Gln87Ala P0C6X7-PRO_0000037322:p.Gln87Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Q A 85 Mon Jun 29 10:24:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Gln87Ala' Tue Jun 30 16:18:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln87Ala' P0C6X7-PRO_0000037322:p.Val104Gly P0C6X7-PRO_0000037322:p.Val104Gly mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V G 85 Mon Jun 29 09:55:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Val104Gly' Tue Jun 30 17:45:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val104Gly' P0C6X7-PRO_0000037322:p.Asp106Ala P0C6X7-PRO_0000037322:p.Asp106Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D A 85 Mon Jun 29 10:07:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Asp106Ala' Tue Jun 30 16:54:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp106Ala' P0C6X7-PRO_0000037322:p.Met247Ala P0C6X7-PRO_0000037322:p.Met247Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain M A 85 Mon Jun 29 10:03:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Met247Ala' Tue Jun 30 19:47:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met247Ala' P0C6X7-PRO_0000037322:p.Lys46Ala P0C6X7-PRO_0000037322:p.Lys46Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A 85 Mon Jun 29 10:16:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Lys46Ala' Tue Jun 30 19:23:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys46Ala' P0C6X7-PRO_0000037322:p.Leu244Ala P0C6X7-PRO_0000037322:p.Leu244Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain L A 85 Mon Jun 29 10:31:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Leu244Ala' Tue Jun 30 20:10:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu244Ala' P0C6X7-PRO_0000037322:p.Tyr30Phe P0C6X7-PRO_0000037322:p.Tyr30Phe mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Y F 85 Mon Jun 29 09:53:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Tyr30Phe' Tue Jun 30 17:20:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr30Phe' P0C6X7-PRO_0000037322:p.Ile40Ala P0C6X7-PRO_0000037322:p.Ile40Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain I A 85 Mon Jun 29 10:34:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Ile40Ala' Tue Jun 30 18:51:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile40Ala' P0C6X7-PRO_0000037317:p.Arg78Ala P0C6X7-PRO_0000037317:p.Arg78Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain R A 96 Mon Jun 29 10:08:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Arg78Ala' Tue Jun 30 15:39:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg78Ala' P0C6X7-PRO_0000037317:p.Lys93Ala P0C6X7-PRO_0000037317:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A 96 Mon Jun 29 10:26:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Lys93Ala' Tue Jun 30 19:06:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys93Ala' P0C6X7-PRO_0000037317:p.His80Ala P0C6X7-PRO_0000037317:p.His80Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H A 96 P0C6X7-PRO_0000037317:p.His80Ala P0C6X7-PRO_0000037317:p.His80Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H A 96 Mon Jun 29 10:28:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.His80Ala' Tue Jun 30 18:22:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His80Ala' P0C6X7-PRO_0000037317:p.Gly94Ala P0C6X7-PRO_0000037317:p.Gly94Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G A 96 Mon Jun 29 10:22:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Gly94Ala' Tue Jun 30 19:40:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' P0C6X7-PRO_0000037317:p.Lys95Ala P0C6X7-PRO_0000037317:p.Lys95Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A 96 Mon Jun 29 10:37:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Lys95Ala' Tue Jun 30 18:34:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys95Ala' P0C6X7-PRO_0000037317:p.Tyr96Ala P0C6X7-PRO_0000037317:p.Tyr96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y A 96 Mon Jun 29 10:29:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Tyr96Ala' Tue Jun 30 18:20:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ala' P0C6X7-PRO_0000037322:p.Asp130Ala P0C6X7-PRO_0000037322:p.Asp130Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A 85 Mon Jun 29 10:13:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Asp130Ala' Tue Jun 30 15:42:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp130Ala' P0C6X7-PRO_0000037322:p.Lys170Ala P0C6X7-PRO_0000037322:p.Lys170Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K A 85 Mon Jun 29 10:22:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Lys170Ala' Tue Jun 30 16:16:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys170Ala' P0C6X7-PRO_0000037322:p.Glu203Ala P0C6X7-PRO_0000037322:p.Glu203Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain E A 85 Mon Jun 29 09:59:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Glu203Ala' Tue Jun 30 16:59:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu203Ala' P0C6X7-PRO_0000037322:p.Asn43Ala P0C6X7-PRO_0000037322:p.Asn43Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain N A 85 Mon Jun 29 10:12:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Asn43Ala' Tue Jun 30 16:00:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn43Ala' P0C6X7-PRO_0000037322:p.Gly73Ala P0C6X7-PRO_0000037322:p.Gly73Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G A 85 Mon Jun 29 10:38:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Gly73Ala' Tue Jun 30 17:37:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly73Ala' P0C6X7-PRO_0000037322:p.Asp99Ala P0C6X7-PRO_0000037322:p.Asp99Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D A 85 Mon Jun 29 10:06:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Asp99Ala' Tue Jun 30 19:34:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp99Ala' P0C6X7-PRO_0000037322:p.Thr58Ala P0C6X7-PRO_0000037322:p.Thr58Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T A 85 Mon Jun 29 09:52:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Thr58Ala' Tue Jun 30 16:44:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Ala' P0C6X7-PRO_0000037322:p.Thr58Asn P0C6X7-PRO_0000037322:p.Thr58Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T N 85 Mon Jun 29 10:30:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Thr58Asn' Tue Jun 30 18:36:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Asn' P0C6X7-PRO_0000037322:p.Thr58Glu P0C6X7-PRO_0000037322:p.Thr58Glu mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T E 85 Mon Jun 29 10:33:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Thr58Glu' Tue Jun 30 16:57:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Glu' P0C6X7-PRO_0000037322:p.Ser188Ala P0C6X7-PRO_0000037322:p.Ser188Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain S A 85 Mon Jun 29 10:32:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Ser188Ala' Tue Jun 30 15:40:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser188Ala' P0C6X7-PRO_0000037322:p.Tyr132Ala P0C6X7-PRO_0000037322:p.Tyr132Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Y A 85 Mon Jun 29 10:02:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Tyr132Ala' Tue Jun 30 19:58:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132Ala' P0C6X7-PRO_0000037322:p.Tyr132Thr P0C6X7-PRO_0000037322:p.Tyr132Thr mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Y T 85 Mon Jun 29 10:36:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Tyr132Thr' Tue Jun 30 17:32:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132Thr' P0C6X7-PRO_0000037322:p.Tyr132Phe P0C6X7-PRO_0000037322:p.Tyr132Phe mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y F 85 Mon Jun 29 10:31:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Tyr132Phe' Tue Jun 30 17:16:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132Phe' P0C6X7-PRO_0000037322:p.Tyr132His P0C6X7-PRO_0000037322:p.Tyr132His mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Y H 85 Mon Jun 29 10:15:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Tyr132His' Tue Jun 30 18:31:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr132His' P0C6X7-PRO_0000037322:p.Tyr30Ala P0C6X7-PRO_0000037322:p.Tyr30Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Y A 85 Mon Jun 29 10:32:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Tyr30Ala' Tue Jun 30 17:13:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr30Ala' P0C6X7-PRO_0000037322:p.Met41Ala P0C6X7-PRO_0000037322:p.Met41Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain M A 85 Mon Jun 29 10:05:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Met41Ala' Tue Jun 30 17:57:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met41Ala' P0C6X7-PRO_0000037322:p.Val44Ala P0C6X7-PRO_0000037322:p.Val44Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V A 85 Mon Jun 29 10:26:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Val44Ala' Tue Jun 30 17:25:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val44Ala' P0C6X7-PRO_0000037322:p.Thr48Ala P0C6X7-PRO_0000037322:p.Thr48Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T A 85 Mon Jun 29 10:19:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037322:p.Thr48Ala' Tue Jun 30 18:50:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr48Ala' P0C6X7-PRO_0000037317:p.Asn40Ala P0C6X7-PRO_0000037317:p.Asn40Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain N A 96 Mon Jun 29 10:37:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Asn40Ala' Tue Jun 30 17:02:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn40Ala' P0C6X7-PRO_0000037317:p.Val42Ala P0C6X7-PRO_0000037317:p.Val42Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V A 96 Mon Jun 29 10:23:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Val42Ala' Tue Jun 30 18:35:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Ala' P0C6X7-PRO_0000037317:p.Met44Ala P0C6X7-PRO_0000037317:p.Met44Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain M A 96 Mon Jun 29 10:40:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Met44Ala' Tue Jun 30 15:45:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met44Ala' P0C6X7-PRO_0000037317:p.Leu45Ala P0C6X7-PRO_0000037317:p.Leu45Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain L A 96 Mon Jun 29 10:21:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Leu45Ala' Tue Jun 30 19:55:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu45Ala' P0C6X7-PRO_0000037317:p.Thr47Ala P0C6X7-PRO_0000037317:p.Thr47Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T A 96 Mon Jun 29 10:03:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Thr47Ala' Tue Jun 30 19:58:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr47Ala' P0C6X7-PRO_0000037317:p.Val57Ala P0C6X7-PRO_0000037317:p.Val57Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V A 96 Mon Jun 29 10:32:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Val57Ala' Tue Jun 30 18:03:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val57Ala' P0C6X7-PRO_0000037317:p.Thr58Ala P0C6X7-PRO_0000037317:p.Thr58Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain T A 96 Mon Jun 29 09:51:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Thr58Ala' Tue Jun 30 20:26:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Ala' P0C6X7-PRO_0000037317:p.Gly70Ala P0C6X7-PRO_0000037317:p.Gly70Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G A 96 Mon Jun 29 10:00:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Gly70Ala' Tue Jun 30 17:54:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly70Ala' P0C6X7-PRO_0000037317:p.Ser72Ala P0C6X7-PRO_0000037317:p.Ser72Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain S A 96 Mon Jun 29 10:21:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0C6X7-PRO_0000037317:p.Ser72Ala' Tue Jun 30 19:56:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser72Ala' ecoli Escherichia coli (strain K12) methylation methylation reaction methylation Tables 2, S1, S2 nsp10-nsp16_cvhsa SARS-CoV NSP16/NSP10 complex SARS-CoV NSP10-NSP16 2'-O-methyltransferase complex NSP10:NSP16 7 unspecified role unspecified role nsp16 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 enzyme enzyme nsp10 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 131 130 physical association physical association sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction 2'-O-methyltransferase complex of the SARS-CoV coronavirus which mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs using S-adenosyl-L-methionine (SAM, CHEBI:15414) as the methyl donor. The cap structure is essential for efficient splicing, nuclear export, translation and mRNA stability. Heterodimer Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death. NSP10 forms a homododecamer in isolation. Accepted 2020-APR-02 by LIVIAP coronavirus