HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Interaction between Core protein of classical swine fever virus with cellular IQGAP1 protein appears essential for virulence in swine. Interaction between Core protein of classical swine fever virus with cellular IQGAP1 protein appears essential for virulence in swine. Virology 2011 imex curation Gladue DP., Holinka LG., Fernandez-Sainz IJ., Prarat MV., O'Donnell V., Vepkhvadze NG., Lu Z., Risatti GR., Borca MV. Virus - Publications including interactions involving viral proteins manuel.borca@ars.usda.gov 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Gladue DP., Holinka LG., Fernandez-Sainz IJ., Prarat MV., O'Donnell V., Vepkhvadze NG., Lu Z., Risatti GR., Borca MV. Virus - Publications including interactions involving viral proteins manuel.borca@ars.usda.gov Accepted 2016-OCT-20 AT 11:45 BST AT 11:45 BST by MGT Swine primary macrophage Virology 2011 imex curation Interaction between Core protein of classical swine fever virus with cellular IQGAP1 protein appears essential for virulence in swine. Interaction between Core protein of classical swine fever virus with cellular IQGAP1 protein appears essential for virulence in swine. Virology 2011 imex curation Gladue DP., Holinka LG., Fernandez-Sainz IJ., Prarat MV., O'Donnell V., Vepkhvadze NG., Lu Z., Risatti GR., Borca MV. Virus - Publications including interactions involving viral proteins manuel.borca@ars.usda.gov 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gladue DP., Holinka LG., Fernandez-Sainz IJ., Prarat MV., O'Donnell V., Vepkhvadze NG., Lu Z., Risatti GR., Borca MV. Virus - Publications including interactions involving viral proteins manuel.borca@ars.usda.gov Accepted 2016-OCT-20 AT 11:45 BST AT 11:45 BST by MGT Swine primary macrophage Virology 2011 imex curation k9iwf9_pig IQGAP1 protein protein pig Sus scrofa (Pig) Pig MSATDEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMGEIGLPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKIELEKYGIQMPAFSKIGGILANELSVDEAALHAAVIAINEAIDHRIPADTFTALKNPNAMLVNLEEPLASTYQDVLYQAKQDKMTNAKNRTESSERERDVYEELLTQAEIQGNINKVNMIAALANVDQALEQGSALALFKILQSPALGLRGLQQQNSDWYLKQLLSDRQQKRESGQAEPLQKEELQSGVDAGNIAAQQYQRRLAAVAAINAAIQKGVAEKTVLELMNPEAQLPQVYPFAAELYQKELATLQQQSPEHSLTHPELSVAVEMLSSVALINRALESGDMNTVWRQLSSSVTGLTNIEEENYQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQTPAAKLEGVLAEVAQHYQDTLIRAKREKAQETQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVDSGDVSRTLGALRSPDVGLYGVIPECGQTYQSDLAEAKKKKLAAGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPDFVQNFMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQACCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVIKIQSLARMHQARKRYRDRLQYFRDHINDIIKIQAFIRANKARDDYKTLINAEDPPMIVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDLNLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMLNKQKGGLKALSKEKREKLEAYQHLFYLLQTNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFKTALQEEIKSKVDQIQEIVTGNPTVIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQALAHEEVRTRLDNSIRNMRAVTDKFLAAIISSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNLLYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQKFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDDLGEVPTIESLIGEGSGNVNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQPGETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKKSKSVKEDSNLTLQEKKEKIQSGLKKLTELGTVDPKNRYQELINDIAKDIRNQRRYRQRRKAELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLASKGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGVQMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK 175CA3C7B7EE0D2D p21530-pro_0000038063 Capsid protein C protein protein csfvb Classical swine fever virus (strain Brescia) Hog cholera virus SDDGASASKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVIAIMLYQPVAA 169 267 1EE967C4F90C386E core-iqgap1-1 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait P21530-PRO_0000038063:p.Val42_Glu44delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain VVE AAA Mon Oct 03 15:07:46 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val42ala_val43ala_glu44ala' Wed May 02 15:21:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_val_glu42-44ala_ala_ala' Tue Jun 30 18:07:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42_Glu44delinsAlaAlaAla' P21530-PRO_0000038063:p.Gly64_Asn68delinsAlaAlaAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain GLYHN AAAAA Wed May 02 15:32:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_leu_tyr_his_asn64-68ala_ala_ala_ala_ala' Tue Jun 30 16:45:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly64_Asn68delinsAlaAlaAlaAlaAla' P21530-PRO_0000038063:p.Thr40_Ile41delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain TI AA Mon Oct 03 14:55:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ala39ala_thr40ala_ile41ala' Wed May 02 15:08:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr_ile40-41ala_ala' Tue Jun 30 17:14:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr40_Ile41delinsAlaAla' P21530-PRO_0000038063:p.Gly45_Lys47delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain GVK AAA Wed May 02 20:29:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_val_lys45-47ala_ala_ala' Tue Jun 30 18:34:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly45_Lys47delinsAlaAlaAla' physical association physical association 1 & table 1 I have changed the mutation but not sure if it is what you are asking for! Is this http://www.ebi.ac.uk/~intact/site/doc/IntActCurationManual2013.pdf 2013 version of the manual the latest? core-iqgap1-2 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association results The IQGAP1 recovered from the library contained amino acids (502–704) of homo sapiens IQGAP1 (NCBI Reference Sequence: AAI39732) amino terminal fused to the GAL4-AD.