HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. J. Gen. Virol. (0022-1317) 2011 imex curation Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. J. Gen. Virol. (0022-1317) 2011 imex curation Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. J. Gen. Virol. (0022-1317) 2011 imex curation Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. J. Gen. Virol. (0022-1317) 2011 imex curation Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. J. Gen. Virol. (0022-1317) 2011 imex curation Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen tag visualisation tag visualisation Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. J. Gen. Virol. (0022-1317) 2011 imex curation Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. Dimerization-driven interaction of hepatitis C virus core protein with NS3 helicase. J. Gen. Virol. (0022-1317) 2011 imex curation Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa tag visualisation tag visualisation Mousseau G., Kota S., Takahashi V., Frick D.N., Strosberg A.D. Virus - Publications including interactions involving viral proteins strosber@scripps.edu Accepted 2013-JUL-26 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. J. Gen. Virol. (0022-1317) 2011 imex curation p26660-pro_0000037615 Serine protease/helicase NS3 protein protein hcvj6 Hepatitis C virus genotype 2a (isolate HC-J6) (HCV) APITAYAQQTRGLLGTIVVSMTGRDKTEQAGEIQVLSTVTQSFLGTTISGVLWTVYHGAGNKTLAGSRGPVTQMYSSAEGDLVGWPSPPGTKSLEPCTCGAVDLYLVTRNADVIPARRRGDKRGALLSPRPLSTLKGSSGGPVLCPRGHAVGVFRAAVCSRGVAKSIDFIPVETLDIVTRSPTFSDNSTPPAVPQTYQVGYLHAPTGSGKSTKVPVAYAAQGYKVLVLNPSVAATLGFGAYLSKAHGINPNIRTGVRTVTTGAPITYSTYGKFLADGGCAGGAYDIIICDECHAVDSTTILGIGTVLDQAETAGVRLTVLATATPPGSVTTPHPNIEEVALGQEGEIPFYGRAIPLSYIKGGRHLIFCHSKKKCDELAAALRGMGLNAVAYYRGLDVSVIPTQGDVVVVATDALMTGFTGDFDSVIDCNVAVTQVVDFSLDPTFTITTQTVPQDAVSRSQRRGRTGRGRLGIYRYVSTGERASGMFDSVVLCECYDAGAAWYELTPAETTVRLRAYFNTPGLPVCQDHLEFWEAVFTGLTHIDAHFLSQTKQSGENFAYLTAYQATVCARAKAPPPSWDVMWKCLTRLKPTLVGPTPLLYRLGSVTNEVTLTHPVTKYIATCMQADLEVMT 1031 1661 198ACA0FDC9C958B p26660-pro_0000037618 Non-structural protein 5A protein protein hcvj6 Hepatitis C virus genotype 2a (isolate HC-J6) (HCV) SGSWLRDVWDWVCTILTDFKNWLTSKLFPKMPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNIWQGTFPINCYTEGQCVPKPAPNFKIAIWRVAASEYAEVTQHGSYHYITGLTTDNLKVPCQLPSPEFFSWVDGVQIHRFAPIPKPFFRDEVSFCVGLNSFVVGSQLPCDPEPDTDVLTSMLTDPSHITAETAARRLARGSPPSEASSSASQLSAPSLRATCTTHGKAYDVDMVDANLFMGGDVTRIESESKVVVLDSLDPMVEERSDLEPSIPSEYMLPKKRFPPALPAWARPDYNPPLVESWKRPDYQPATVAGCALPPPKKTPTPPPRRRRTVGLSESSIADALQQLAIKSFGQPPPSGDSGLSTGADAADSGSRTPPDELALSETGSISSMPPLEGEPGDPDLEPEQVELQPPPQGGVVTPGSGSGSWSTCSEEDDSVVCC 1977 2442 FC4C64A76DC0F6D7 p27958-pro_0000037566 Core protein precursor protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) STNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARRPEGRTWAQPGYPWPLYGNEGCGWAGWLLSPRGSRPSWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGAARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTVPASA 2 191 7C9059C0D1D2732E ns3-core-1 4 Core 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) NS3 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 10 direct interaction direct interaction 2A p26660_pr-p27958_p-1 3 NS3 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) Core 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 19 16 direct interaction direct interaction 2B and 2C core-ns3-1 1 NS3 5 unspecified role unspecified role prey prey human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma Core 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 2E ns5a-core-1 1 NS5A 6 unspecified role unspecified role prey prey human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma Core 7 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 2E p26660_pro-p27958_pr-1 2 Core 7 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) NS3 5 unspecified role unspecified role unspecified role unspecified role gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 pr-p27958_p-1 2 Core 7 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 p27958-pro-2 2 Core 7 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 p27958-pro-1 2 Core 7 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 p26660-pro-1 2 NS3 5 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 3