HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain. Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain. Journal of virology 2010 imex curation Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Re-IP. Protein complexes were re-immunoprecipitated from the FLAG elutes using anti-myc antibody and detected for the presence of ERK2-EGFP by Western blotting Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca Accepted 2016-DEC-07 AT 09:50 GMT AT 09:50 GMT by MGT Journal of virology 2010 imex curation Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain. Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain. Journal of virology 2010 imex curation Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca Accepted 2016-DEC-07 AT 09:50 GMT AT 09:50 GMT by MGT Journal of virology 2010 imex curation Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain. Pseudorabies virus tegument protein Us2 recruits the mitogen-activated protein kinase extracellular-regulated kinase (ERK) to membranes through interaction with the ERK common docking domain. Journal of virology 2010 imex curation Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kang MH., Banfield BW. Virus - Publications including interactions involving viral proteins Interactions in Figure 1,5 & 6 have bnot been annotated since it is not clear if RAT or Mouse ERK2 was used. Us2 expression plasmids were derived from the Becker strain genomic DNA; as this strain has no entries in Uniprot or NCBI databases a Us2 identifier from the top level TaxID has been used. The US2 molecule is described in Ref 3 as having 263 amino acids, and has a C-terminal CAAX sequence. No current version of the US2 protein has 263 amino acids nor a C-terminal CAAX sequence. Regions CR1 and CR2 (sequence from Ref3) appear to be identical to US2 proteins in current Uniprot proteins, but the sequence surrounding CR3 (residues in region of 150-161) differs. bruce.banfield@queensu.ca Accepted 2016-DEC-07 AT 09:50 GMT AT 09:50 GMT by MGT Journal of virology 2010 imex curation g3g926_9alph US2 protein protein pseudorabies virus pseudorabies virus MGVTAITVVTLMDGSGRIPAFVGEAHPDLWKVLTEWCYASLVQQRRAADEDSPRQHVVLRSSEIAPGSLALLPRATRPVVRTRSDPTAPFYITTETHELTRRPPADGSKPGEPLRISPPPRLDTEWSSVLNGIQYLNSGARGTAPVHLWILGAADLCDQVLLAASRSTAAGAPGAPTGARLTRRRPGLTDADALDVIVAGIPATRAMFARVHNRSWRHAGEWTEALHAQIVTRGDVRRRRGGRGNGRERAPRCTIS F1AA7B6AF394C567 mk01_mouse Mitogen-activated protein kinase 1 Mapk1 Erk2 Mapk Prkm1 Extracellular signal-regulated kinase 2 Mitogen-activated protein kinase 2 MAP kinase isoform p42 ERT1 protein protein mouse Mus musculus Mouse MAAAAAAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNLNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS DIP protein P63085 original sequence version: 120 DIP protein P63085 original sequence version: 94 DIP protein P63085 original sequence version: 88 DIP protein P63085 original sequence version: 103 DIP protein P63085 original sequence version: 121 DIP protein P63085 original sequence version: 101 3BBCF22471EDBA0B mp2k1_human Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAPK/ERK kinase 1 MAP2K1 MEK1 PRKMK1 protein protein human Homo sapiens Human MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV DIP protein Q02750 original sequence version: 96 DIP protein Q02750 original sequence version: 93 DIP protein Q02750-1 original sequence version: 151 DIP protein Q02750 original sequence version: 151 DIP protein Q02750-1 original sequence version: 166 0344118FFC842D51 us2-erk2-1 3 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association 2B mapk1-us2-1 2 5 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2C us2-us2-1 2 4 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined self interaction self interaction 3B us2-mapk1-1 2 4 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 3C erk2-us2-2 3 5 unspecified role unspecified role prey prey P63085:p.[Asp316Ala;Asp319Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A Fri Jul 22 11:05:42 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly316ala_gly319ala' Wed May 02 19:31:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp316ala,asp319ala' Tue Jun 30 19:26:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp316Ala;Asp319Ala]' P63085:p.Tyr261Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y N Wed May 02 15:22:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr261asn' Tue Jun 30 17:36:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr261Asn' P63085:p.Asn236Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N K Wed May 02 16:01:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn236lys' Tue Jun 30 20:03:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn236Lys' P63085:p.His230Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H R Wed May 02 15:51:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his230arg' Tue Jun 30 16:49:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His230Arg' 4 unspecified role unspecified role bait bait physical association physical association 4B map2k1-mapk1-3 3 6 unspecified role unspecified role bait bait Q02750:p.Lys97Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 17:18:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys97met' Wed May 02 19:33:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys97met' Tue Jun 30 18:37:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys97Met' 5 unspecified role unspecified role prey prey P63085:p.His230Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H R Wed May 02 19:10:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his230arg' Tue Jun 30 15:41:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His230Arg' P63085:p.Tyr261Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y N Fri Jul 22 10:59:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr260asn' Wed May 02 18:48:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr261asn' Tue Jun 30 18:27:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr261Asn' P63085:p.Asn236Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N K Wed May 02 16:55:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn236lys' Tue Jun 30 18:24:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn236Lys' P63085:p.[Asp316Ala;Asp319Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A Tue Jan 24 14:07:53 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'asp316ala,asp219ala' Wed May 02 15:53:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp316ala,asp319ala' Tue Jun 30 20:02:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp316Ala;Asp319Ala]' amino acid number is different from the one in the publication physical association physical association 4B us2-erk2-2 2 5 unspecified role unspecified role prey prey P63085:p.[Asp316Pro;Asp319Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D P certain certain sequence position certain certain certain sequence position certain D A Fri Jul 22 11:00:44 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'asp316ala_asp319pro' Wed May 02 18:36:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp316pro,asp319ala' Tue Jun 30 20:21:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp316Pro;Asp319Ala]' 4 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4C mapk1-us2-2 3 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait G3G926:p.Arg184_Arg185delinsLysLys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RR KK Wed May 02 16:01:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg184-185lys_lys' Tue Jun 30 20:16:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg184_Arg185delinsLysLys' G3G926:p.Arg184_Arg185delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RR AA Wed May 02 17:50:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg184-185ala_ala' Tue Jun 30 16:59:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg184_Arg185delinsAlaAla' G3G926:p.Arg183_Arg185delinsAlaAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain RRR AAA Wed May 02 17:46:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg_arg183-185ala_ala_ala' Tue Jun 30 19:36:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg183_Arg185delinsAlaAlaAla' G3G926:p.Leu181_Arg185delinsAlaAlaAlaAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LTRRR AAAAA Wed May 02 19:26:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_thr_arg_arg_arg181-185ala_ala_ala_ala_ala' Tue Jun 30 19:42:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu181_Arg185delinsAlaAlaAlaAlaAla' physical association physical association 2E us2-mapk1-3 1 4 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 3C ERK2-EGFP was immunoprecipitated specifically in the complexes containing oligomeric Us2. us2-mapk1-2 2 4 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3C