HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. J. Virol. (0022-538X) 2010 imex curation Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw. michlai@gate.sinica.edu.tw Accepted 2013-NOV-13 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. J. Virol. (0022-538X) 2010 imex curation Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. J. Virol. (0022-538X) 2010 imex curation Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw. michlai@gate.sinica.edu.tw Accepted 2013-NOV-13 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. J. Virol. (0022-538X) 2010 imex curation Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. J. Virol. (0022-538X) 2010 imex curation Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen Y.C., Su W.C., Huang J.Y., Chao T.C., Jeng K.S., Machida K., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@gate.sinica.edu.tw. michlai@gate.sinica.edu.tw Accepted 2013-NOV-13 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. J. Virol. (0022-538X) 2010 imex curation q99ib8-pro_0000045602 Non-structural protein 5A protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV SGSWLRDVWDWVCTILTDFKNWLTSKLFPKLPGLPFISCQKGYKGVWAGTGIMTTRCPCGANISGNVRLGSMRITGPKTCMNTWQGTFPINCYTEGQCAPKPPTNYKTAIWRVAASEYAEVTQHGSYSYVTGLTTDNLKIPCQLPSPEFFSWVDGVQIHRFAPTPKPFFRDEVSFCVGLNSYAVGSQLPCEPEPDADVLRSMLTDPPHITAETAARRLARGSPPSEASSSVSQLSAPSLRATCTTHSNTYDVDMVDANLLMEGGVAQTEPESRVPVLDFLEPMAEEESDLEPSIPSECMLPRSGFPRALPAWARPDYNPPLVESWRRPDYQPPTVAGCALPPPKKAPTPPPRRRRTVGLSESTISEALQQLAIKTFGQPPSSGDAGSSTGAGAAESGGPTSPGEPAPSETGSASSMPPLEGEPGDPDLESDQVELQPPPQGGGVAPGSGSGSWSTCSEEDDTTVCC 1977 2442 0BDEA498491643D8 plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS DIP protein P53350 original sequence version: 169 DIP protein P53350 original sequence version: 170 DIP protein P53350 original sequence version: 164 DIP protein P53350 original sequence version: 154 DIP protein P53350 original sequence version: 91 DIP protein P53350 original sequence version: 145 DIP protein P53350 original sequence version: 121 DIP protein P53350 original sequence version: 118 DIP protein P53350 original sequence version: 123 DIP protein P53350 original sequence version: 119 DIP protein P53350 original sequence version: 138 DIP protein P53350 original sequence version: 163 DIP protein P53350 original sequence version: 178 178C2F13C10E8206 ns5a-plk1-3 1 NS5A 4 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PLK1 5 unspecified role unspecified role prey prey physical association physical association Fig. 4A ns5a-plk1-1 3 NS5A 4 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PLK1 5 unspecified role unspecified role prey prey physical association physical association Fig. 4B ns5a-plk1-2 3 PLK1 5 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NS5A 4 unspecified role unspecified role prey prey physical association physical association Fig. 4C plk1-ns5a-1 2 PLK1 5 unspecified role unspecified role neutral component neutral component NS5A 4 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4D