UniProt UniProt http://www.uniprot.org Uncoating of human immunodeficiency virus type 1 requires prolyl isomerase Pin1. Uncoating of human immunodeficiency virus type 1 requires prolyl isomerase Pin1. J. Biol. Chem. (0021-9258) 2010 imex curation Misumi S., Inoue M., Dochi T., Kishimoto N., Hasegawa N., Takamune N., Shoji S. Virus - Publications including interactions involving viral proteins Origin of GST-Pin1 according Ref. 21 (PMID 9499405). HIV-1 capsid protein p24 has been mapped to the UniProt HIV-1 virus reference strain. misumi@gpo.kumamoto-u.ac.jp 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Misumi S., Inoue M., Dochi T., Kishimoto N., Hasegawa N., Takamune N., Shoji S. Virus - Publications including interactions involving viral proteins Purified HIV-1 CA cores have been used. Phosphorylation detected by using a anti-pS17/P17 antibody. Phosphorylation of other Ser-Pro motifs in HIV-1 CA cores has been excluded by MS analysis. Origin of GST-Pin1 according Ref. 21 (PMID 9499405). HIV-1 capsid protein p24 has been mapped to the UniProt HIV-1 virus reference strain. misumi@gpo.kumamoto-u.ac.jp Accepted 2014-OCT-27 by ORCHARD J. Biol. Chem. (0021-9258) 2010 imex curation pin1_human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 PIN1 protein protein human Homo sapiens Human MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE DIP protein Q13526 original sequence version: 65 DIP protein Q13526 original sequence version: 64 DIP protein Q13526 original sequence version: 163 DIP protein Q13526 original sequence version: 156 DIP protein Q13526 original sequence version: 117 35391AF40B7D1E13 p04591-pro_0000038594 Capsid protein p24 gag Pr55Gag protein protein hv1h2 Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) HIV-1 PIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVL 133 363 2D2EB56755AB9312 pin1-gag-1 1 3 unspecified role unspecified role prey prey phosphoser-16 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 3a and c ALP (alkaline phosphatase) - removes phosphate groups from HIV-1 CA core p24 phosphorylated on Ser-16 Q13526 increases activity of P04591-PRO_0000038594