DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Virology journal 2009 imex curation Wang Q., Li C., Zhang Q., Wang T., Li J., Guan W., Yu J., Liang M., Li D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus mifangl@sina.com, lidx@chinacdc.cn Only protein-protein interactions imex curation in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp Wang Q., Li C., Zhang Q., Wang T., Li J., Guan W., Yu J., Liang M., Li D. Virus - Publications including interactions involving viral proteins mifangl@sina.com, lidx@chinacdc.cn Accepted 2020-JUN-15 AT 19:11 BST AT 19:11 BST by KALPANAP Virology journal 2009 Only protein-protein interactions imex curation imex curation Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Virology journal 2009 imex curation Wang Q., Li C., Zhang Q., Wang T., Li J., Guan W., Yu J., Liang M., Li D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus mifangl@sina.com, lidx@chinacdc.cn Only protein-protein interactions imex curation human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast confocal microscopy confocal microscopy anti-tag immunocytochemistry anti-tag immunocytochemistry Wang Q., Li C., Zhang Q., Wang T., Li J., Guan W., Yu J., Liang M., Li D. Virus - Publications including interactions involving viral proteins mifangl@sina.com, lidx@chinacdc.cn Accepted 2020-JUN-15 AT 19:11 BST AT 19:11 BST by KALPANAP Virology journal 2009 Only protein-protein interactions imex curation imex curation Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Virology journal 2009 imex curation Wang Q., Li C., Zhang Q., Wang T., Li J., Guan W., Yu J., Liang M., Li D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus mifangl@sina.com, lidx@chinacdc.cn Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay experimental particp experimental participant identification experimental particp Wang Q., Li C., Zhang Q., Wang T., Li J., Guan W., Yu J., Liang M., Li D. Virus - Publications including interactions involving viral proteins mifangl@sina.com, lidx@chinacdc.cn Accepted 2020-JUN-15 AT 19:11 BST AT 19:11 BST by KALPANAP Virology journal 2009 Only protein-protein interactions imex curation imex curation k2c1_human Keratin, type II cytoskeletal 1 KRT1 KRTA Keratin-1 Cytokeratin-1 67 kDa cytokeratin Hair alpha protein Type-II keratin Kb1 protein protein human Homo sapiens Human MSRQFSSRSGYRSGGGFSSGSAGIINYQRRTTSSSTRRSGGGGGRFSSCGGGGGSFGAGGGFGSRSLVNLGGSKSISISVARGGGRGSGFGGGYGGGGFGGGGFGGGGFGGGGIGGGGFGGFGSGGGGFGGGGFGGGGYGGGYGPVCPPGGIQEVTINQSLLQPLNVEIDPEIQKVKSREREQIKSLNNQFASFIDKVRFLEQQNQVLQTKWELLQQVDTSTRTHNLEPYFESFINNLRRRVDQLKSDQSRLDSELKNMQDMVEDYRNKYEDEINKRTNAENEFVTIKKDVDGAYMTKVDLQAKLDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNRSLDLDSIIAEVKAQYEDIAQKSKAEAESLYQSKYEELQITAGRHGDSVRNSKIEISELNRVIQRLRSEIDNVKKQISNLQQSISDAEQRGENALKDAKNKLNDLEDALQQAKEDLARLLRDYQELMNTKLALDLEIATYRTLLEGEESRMSGECAPNVSVSVSTSHTTISGGGSRGGGGGGYGSGGSSYGSGGGSYGSGGGGGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR CE5DDE97388F5017 prs10_human 26S proteasome regulatory subunit 10B PSMC6 SUG2 Proteasome 26S subunit ATPase 6 Proteasome subunit p42 26S proteasome AAA-ATPase subunit RPT4 protein protein human Homo sapiens Human MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVSSSIVDKYIGESARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATNRPDTLDPALLRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEAGMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV DIP protein P62333 original sequence version: 71 B26421295742CACD ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid 43FC8750F1253034 k1c10_human Keratin, type I cytoskeletal 10 KRT10 Cytokeratin-10 KPP Keratin-10 protein protein human Homo sapiens Human MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGIFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY 4941ECD2AE46D417 krt1-1 3 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 fingerprinting peptide massfingerprinting fingerprinting experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast 4 fingerprinting peptide massfingerprinting fingerprinting experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast 5 fingerprinting peptide massfingerprinting fingerprinting experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast association association Fig2b n-55 1 6 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4 n-psmc6-1 2 6 anti-tag immunocytochemistry anti-tag immunocytochemistry immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast 5 anti-tag immunocytochemistry anti-tag immunocytochemistry immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast colocalization colocalization Fig 5